##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921420_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3214210 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.302841133591148 31.0 31.0 34.0 26.0 34.0 2 30.35339103543328 31.0 31.0 34.0 26.0 34.0 3 30.403500393564826 33.0 31.0 34.0 26.0 34.0 4 33.857850918266074 37.0 35.0 37.0 30.0 37.0 5 33.84512897414917 37.0 35.0 37.0 30.0 37.0 6 33.89265511587606 37.0 35.0 37.0 30.0 37.0 7 33.80456908540512 37.0 35.0 37.0 29.0 37.0 8 33.76993382510788 37.0 35.0 37.0 29.0 37.0 9 35.31701817865043 39.0 37.0 39.0 29.0 39.0 10-11 35.2036813089375 39.0 35.0 39.0 29.0 39.0 12-13 35.083191048500254 39.0 35.0 39.0 28.0 39.0 14-15 36.209324219637175 40.0 36.0 41.0 27.0 41.0 16-17 36.15129829724878 40.0 36.0 41.0 27.0 41.0 18-19 36.09132461786878 40.0 36.0 41.0 27.0 41.0 20-21 36.02729162064706 40.0 36.0 41.0 27.0 41.0 22-23 35.913752990626 40.0 36.0 41.0 27.0 41.0 24-25 35.80873573910853 40.0 36.0 41.0 27.0 41.0 26-27 35.66033426565159 40.0 36.0 41.0 26.5 41.0 28-29 35.520132474231616 40.0 36.0 41.0 25.5 41.0 30-31 35.38332669613995 40.0 35.5 41.0 25.0 41.0 32-33 35.20636875001944 39.0 35.0 41.0 24.5 41.0 34-35 35.04562271911294 39.0 35.0 41.0 24.0 41.0 36-37 34.87861045171286 39.0 35.0 41.0 24.0 41.0 38-39 35.12426086036693 39.0 35.0 41.0 24.0 41.0 40-41 35.252719019603575 40.0 35.0 41.0 24.0 41.0 42-43 35.098275003811196 40.0 35.0 41.0 23.5 41.0 44-45 34.98732425697139 40.0 35.0 41.0 23.0 41.0 46-47 34.84001309808631 39.5 35.0 41.0 22.5 41.0 48-49 34.699513877438 39.0 35.0 41.0 21.5 41.0 50-51 32.99192616537189 37.0 32.5 39.5 17.0 40.0 52-53 33.14827562604808 37.5 32.5 39.5 16.5 40.0 54-55 33.999499721549 38.5 34.0 40.5 18.0 41.0 56-57 34.00825552779688 38.5 34.0 41.0 18.0 41.0 58-59 33.82529004016539 38.0 34.0 40.5 16.5 41.0 60-61 33.57421357036411 38.0 34.0 40.0 13.5 41.0 62-63 33.27910217440677 37.0 33.5 40.0 12.5 41.0 64-65 32.988007317505705 37.0 33.0 40.0 9.5 41.0 66-67 32.64496485917224 36.0 33.0 39.0 7.5 41.0 68-69 32.27712283889354 35.5 33.0 39.0 6.0 41.0 70-71 31.89873250347675 35.0 32.0 38.5 2.0 40.5 72-73 31.54441044611273 35.0 32.0 37.5 2.0 40.0 74-75 31.151009268218317 35.0 32.0 37.0 2.0 39.0 76-77 30.78358010210907 35.0 31.5 36.5 2.0 39.0 78-79 30.45285917846065 35.0 31.0 36.0 2.0 38.0 80-81 30.117885887978694 35.0 31.0 35.5 2.0 37.0 82-83 29.830591031699857 35.0 31.0 35.0 2.0 37.0 84-85 29.60574464642945 35.0 31.0 35.0 2.0 36.0 86-87 29.397136154762755 35.0 31.0 35.0 2.0 36.0 88-89 29.209945367601993 35.0 30.5 35.0 2.0 36.0 90-91 28.968691840296685 34.0 30.0 35.0 2.0 35.0 92-93 28.785280053263477 34.0 30.0 35.0 2.0 35.0 94-95 28.586669197096644 34.0 30.0 35.0 2.0 35.0 96-97 28.34450829286201 34.0 30.0 35.0 2.0 35.0 98-99 28.119903179941574 34.0 29.5 35.0 2.0 35.0 100-101 27.079603230653877 33.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118763.0 3 20080.0 4 13815.0 5 10368.0 6 9454.0 7 9601.0 8 9990.0 9 10432.0 10 11448.0 11 12094.0 12 12168.0 13 12319.0 14 12789.0 15 13210.0 16 13442.0 17 13893.0 18 13513.0 19 12739.0 20 12932.0 21 13644.0 22 15225.0 23 17431.0 24 20632.0 25 24074.0 26 28672.0 27 34079.0 28 40788.0 29 48609.0 30 57537.0 31 69943.0 32 87933.0 33 114514.0 34 160913.0 35 233127.0 36 388063.0 37 696236.0 38 702983.0 39 116751.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.58622973762095 17.489457150647887 12.760583003694201 25.16373010803697 2 16.13602538436563 18.953072356835495 39.29328212712666 25.617620131672215 3 19.65031262441088 23.06376591720021 28.828617950153784 28.457303508235128 4 13.836073698009002 15.069572442097156 35.481618611838414 35.61273524805543 5 14.721016323137171 36.873680812804515 33.789556939862344 14.615745924195975 6 34.60239785139968 34.026133088874346 16.722787056494113 14.648682003231862 7 29.825610630395605 31.346025550388834 20.214031803562715 18.61433201565285 8 27.34729238102618 34.62343587747231 19.309964111262865 18.71930763023865 9 27.289997887072587 15.55382192471817 18.822946951829923 38.33323323637932 10-11 26.458543871728146 24.77809544925879 27.021343633669026 21.74201704534404 12-13 26.65104211564429 22.136473222857735 27.049010695794596 24.163473965703375 14-15 23.869802238833262 23.66532261139424 24.170864602240513 28.294010547531983 16-17 24.298603259548614 26.792560344347354 25.015895037667274 23.892941358436754 18-19 24.36925208351032 26.281046489953003 26.120191184108528 23.229510242428148 20-21 24.757472202625117 25.36660418760151 26.217151421432444 23.658772188340926 22-23 24.66051982347591 25.13837244989115 25.88299396209924 24.318113764533695 24-25 24.489455768603186 25.38336045601589 25.78524406757135 24.341939707809573 26-27 24.264054619455784 25.610292373928413 25.87445156158289 24.251201445032915 28-29 24.296532932034527 25.856980354064667 25.679996253382733 24.166490460518077 30-31 24.668660663931078 25.53251006398164 25.626339433305457 24.17248983878182 32-33 24.40841039302817 25.522631428531867 25.604852291037272 24.46410588740269 34-35 24.629925018112676 25.66150353642503 25.6718336343945 24.0367378110678 36-37 24.66678292311969 25.575387772569396 25.700381608436544 24.057447695874362 38-39 24.56110574221543 25.706536991760387 25.6100368825502 24.12232038347398 40-41 24.63788542417758 25.5179060210994 25.702484931500457 24.141723623222564 42-43 24.43290719758233 25.472755959324555 25.597249725984323 24.49708711710879 44-45 24.294992488803338 25.548074230166627 25.76065379749864 24.396279483531398 46-47 24.531319673936302 25.46915569664624 25.583681042236755 24.415843587180706 48-49 24.310028520125165 25.65271405640529 25.633902213641125 24.40335520982842 50-51 24.34489249695733 25.667521321297826 25.714449138957928 24.273137042786917 52-53 24.564530583935348 25.506043532134758 25.699498905072517 24.22992697885738 54-55 24.092100765838705 25.35032557924958 26.031268512989897 24.526305141921817 56-57 24.17758607279064 25.55303921976652 25.78148904832783 24.487885659115012 58-59 24.344900758787404 25.523818311869867 25.819642682068217 24.31163824727451 60-61 24.464459655547646 25.456830601263253 25.933469184734157 24.14524055845494 62-63 24.369391879695872 25.497773040620224 25.922994933811594 24.209840145872306 64-65 24.1582898456703 25.262052170855192 25.95776645055718 24.621891532917324 66-67 24.22601928080325 25.46030490246366 25.78098662114311 24.532689195589978 68-69 24.226005878306157 25.411112049717165 25.909745650574006 24.45313642140267 70-71 24.177423434146423 25.234314505113897 25.917829532019255 24.670432528720426 72-73 24.129838912276043 25.50065236756751 25.776311171537447 24.593197548618996 74-75 24.145240670379916 25.53384471048801 25.850535948608776 24.470378670523292 76-77 24.136520405267486 25.26152058058855 25.877403982889284 24.72455503125468 78-79 24.084953102516646 25.226015301559755 26.01502097452551 24.674010621398086 80-81 24.219541773240003 25.44222185742924 25.736472610828088 24.601763758502678 82-83 24.20205551845346 25.48697279948718 25.829652743091998 24.481318938967362 84-85 24.20849687476666 25.441895071286375 25.81277344914865 24.536834604798315 86-87 24.00013283593655 25.623911840593134 25.809180553504895 24.56677476996542 88-89 23.95947844012231 25.361552974111433 25.891762589944882 24.78720599582137 90-91 24.13468549743355 25.222988743728088 25.956963963684455 24.685361795153902 92-93 24.172029672675386 25.179131962455603 25.99854658210587 24.650291782763137 94-95 24.211667566388392 25.118499744429133 25.989124750081633 24.680707939100845 96-97 24.063664805472808 24.984079432560414 26.111648553707667 24.84060720825911 98-99 23.884418309066845 24.97517845939308 26.06520581298029 25.075197418559775 100-101 24.20356541355189 24.922888463059984 26.087814139560617 24.78573198382751 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 42.0 1 37.5 2 47.0 3 77.5 4 91.0 5 105.5 6 172.0 7 316.5 8 469.0 9 540.5 10 508.5 11 472.0 12 498.0 13 559.0 14 624.0 15 692.0 16 789.0 17 914.5 18 1002.5 19 1063.0 20 1177.0 21 1302.5 22 1422.0 23 1594.0 24 1719.5 25 1974.0 26 2501.0 27 3157.5 28 4216.0 29 5999.5 30 8804.5 31 13293.0 32 19754.5 33 25915.0 34 31666.0 35 38853.5 36 48418.5 37 57901.5 38 66505.5 39 74891.0 40 82088.0 41 88989.0 42 96485.5 43 104605.0 44 112570.0 45 121713.0 46 128409.5 47 134076.5 48 140873.0 49 144569.5 50 145389.5 51 145490.0 52 145121.0 53 143897.5 54 141677.0 55 135524.5 56 126174.5 57 114583.0 58 102913.5 59 90786.0 60 76026.5 61 63014.5 62 51892.0 63 40142.5 64 29462.0 65 21540.5 66 16143.0 67 12414.0 68 9348.5 69 7064.0 70 5428.0 71 4080.0 72 2992.5 73 2188.0 74 1652.0 75 1271.0 76 946.0 77 643.0 78 415.0 79 296.0 80 220.0 81 173.0 82 141.5 83 102.5 84 91.0 85 85.5 86 71.5 87 54.5 88 54.5 89 48.0 90 39.0 91 32.0 92 25.5 93 25.0 94 22.5 95 17.0 96 8.5 97 8.5 98 10.5 99 9.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04996562141241549 2 0.05721468105693156 3 0.04881448318560393 4 0.05609465467408788 5 0.047632233114824486 6 0.018138205033274117 7 0.014995908792518223 8 0.020751599926576048 9 0.02050270517483301 10-11 0.022478307266793396 12-13 0.0277206529753812 14-15 0.044194374356373724 16-17 0.029338468861710966 18-19 0.02373833694749254 20-21 0.026973968720152074 22-23 0.04679221332769172 24-25 0.04167431499497543 26-27 0.03098739659200861 28-29 0.020051583437298747 30-31 0.012366957977232352 32-33 0.00950466833218738 34-35 0.00934910911234798 36-37 0.0016022599643458267 38-39 0.006969053048805149 40-41 0.004277878545583518 42-43 0.21219833178292644 44-45 0.18665550788529686 46-47 0.19247342270729043 48-49 0.19054448838128188 50-51 0.1895177975303418 52-53 0.1939823471397326 54-55 0.1916022910761898 56-57 0.20233587724510843 58-59 0.1989446862526095 60-61 0.20342479178398426 62-63 0.20138696600408812 64-65 0.20221142986923693 66-67 0.19290898852284077 68-69 0.19112005749468766 70-71 0.17573525065257092 72-73 0.18343543203462126 74-75 0.18293764253113518 76-77 0.1807753693753675 78-79 0.20622485774109348 80-81 0.21502950958400355 82-83 0.21292946011617161 84-85 0.20812268022313415 86-87 0.2254519773132434 88-89 0.22327414823549177 90-91 0.22255857582423053 92-93 0.22669645107195857 94-95 0.23841006032586545 96-97 0.2385033958577691 98-99 0.22767647415694683 100-101 0.2381144978081706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3214210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.13171777172384 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36133717486298 44.19786081917441 2 12.190331268608183 13.92909131163886 3 4.098770769484882 7.02509444439605 4 1.9010856133941105 4.344491468972673 5 1.1028325575498055 3.1503359213701945 6 0.712170038358995 2.4412498582202304 7 0.4763460308418001 1.905012689701421 8 0.3492175394394106 1.5961118323350596 9 0.25844223695158125 1.3288724047629648 >10 1.4535505853639992 14.715813007676132 >50 0.0727067146978502 2.786342170410538 >100 0.022115663337218178 2.0430819300307594 >500 8.750456873850036E-4 0.3860698863870605 >1k 2.1876142173787253E-4 0.15057225492361853 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.1111843967880134E-5 2 0.0 0.0 0.0 0.0 3.1111843967880134E-5 3 0.0 0.0 0.0 0.0 3.1111843967880134E-5 4 0.0 0.0 0.0 0.0 3.1111843967880134E-5 5 0.0 0.0 0.0 0.0 3.1111843967880134E-5 6 0.0 0.0 0.0 0.0 6.222368793576027E-5 7 0.0 0.0 0.0 0.0 6.222368793576027E-5 8 0.0 0.0 0.0 0.0 6.222368793576027E-5 9 0.0 0.0 0.0 0.0 6.222368793576027E-5 10-11 0.0 0.0 0.0 0.0 6.222368793576027E-5 12-13 0.0 0.0 0.0 0.0 1.866710638072808E-4 14-15 0.0 0.0 0.0 0.0 2.3333882975910098E-4 16-17 0.0 0.0 0.0 0.0 3.1111843967880133E-4 18-19 0.0 0.0 0.0 0.0 3.4223028364668145E-4 20-21 0.0 0.0 0.0 0.0 4.044539715824417E-4 22-23 0.0 1.5555921983940067E-5 0.0 0.0 4.6667765951820196E-4 24-25 0.0 3.1111843967880134E-5 0.0 0.0 4.977895034860821E-4 26-27 0.0 3.1111843967880134E-5 0.0 7.777960991970033E-5 4.977895034860821E-4 28-29 0.0 6.222368793576027E-5 0.0 0.0012600296806991452 4.977895034860821E-4 30-31 0.0 6.222368793576027E-5 0.0 0.006440151701351188 4.977895034860821E-4 32-33 0.0 6.222368793576027E-5 0.0 0.01883822152255142 4.977895034860821E-4 34-35 0.0 6.222368793576027E-5 0.0 0.03521860737164031 5.289013474539622E-4 36-37 0.0 6.222368793576027E-5 0.0 0.062099240559888746 5.289013474539622E-4 38-39 0.0 6.222368793576027E-5 0.0 0.10876700651170894 5.289013474539622E-4 40-41 0.0 6.222368793576027E-5 0.0 0.1640683091646159 5.911250353897225E-4 42-43 0.0 6.222368793576027E-5 0.0 0.22059852965425408 6.377928013415427E-4 44-45 0.0 6.222368793576027E-5 0.0 0.2911757476953901 6.844605672933629E-4 46-47 0.0 6.222368793576027E-5 0.0 0.3734665749904331 7.311283332451831E-4 48-49 1.5555921983940067E-5 6.222368793576027E-5 0.0 0.46362869880934976 7.777960991970033E-4 50-51 3.1111843967880134E-5 6.222368793576027E-5 0.0 0.5618332342939634 8.089079431648834E-4 52-53 3.1111843967880134E-5 6.222368793576027E-5 0.0 0.662635608749895 8.089079431648834E-4 54-55 3.1111843967880134E-5 6.222368793576027E-5 0.0 0.7825095435581371 8.089079431648834E-4 56-57 3.1111843967880134E-5 6.222368793576027E-5 0.0 0.9197127754564886 8.244638651488236E-4 58-59 3.1111843967880134E-5 6.222368793576027E-5 0.0 1.068551836998827 8.866875530845837E-4 60-61 3.1111843967880134E-5 6.222368793576027E-5 0.0 1.23545132396452 9.022434750685239E-4 62-63 3.1111843967880134E-5 6.222368793576027E-5 0.0 1.415837795290289 9.022434750685239E-4 64-65 3.1111843967880134E-5 6.222368793576027E-5 0.0 1.6072845271466394 9.333553190364039E-4 66-67 3.1111843967880134E-5 6.222368793576027E-5 0.0 1.8304342280062598 9.333553190364039E-4 68-69 3.1111843967880134E-5 6.222368793576027E-5 0.0 2.078924525777718 9.333553190364039E-4 70-71 3.1111843967880134E-5 6.222368793576027E-5 0.0 2.3407151368454455 9.333553190364039E-4 72-73 3.1111843967880134E-5 6.222368793576027E-5 0.0 2.6150749328761966 0.0010266908509400444 74-75 3.1111843967880134E-5 6.222368793576027E-5 0.0 2.9001994269198343 0.0010266908509400444 76-77 3.1111843967880134E-5 6.222368793576027E-5 0.0 3.221506995498116 0.0010266908509400444 78-79 3.1111843967880134E-5 6.222368793576027E-5 0.0 3.587320056872451 0.0010266908509400444 80-81 3.1111843967880134E-5 6.222368793576027E-5 0.0 3.9660600894154396 0.0010578026949079247 82-83 3.1111843967880134E-5 6.222368793576027E-5 0.0 4.363902794154707 0.0011200263828436848 84-85 3.1111843967880134E-5 6.222368793576027E-5 0.0 4.782528210664518 0.0011200263828436848 86-87 4.6667765951820205E-5 6.222368793576027E-5 0.0 5.235127760787254 0.001166694148795505 88-89 6.222368793576027E-5 7.777960991970033E-5 0.0 5.734052846578164 0.0012289178367312654 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2635 0.0 48.08818 1 GTATCAA 4575 0.0 42.634228 1 TCAACGC 5350 0.0 35.306713 4 CAACGCA 5480 0.0 34.46646 5 ATCAACG 5695 0.0 33.251186 3 TATCAAC 5765 0.0 32.847954 2 AACGCAG 5970 0.0 31.941595 6 GTACATG 10255 0.0 28.599634 1 TACATGG 10460 0.0 27.677883 2 ACGCAGA 7290 0.0 25.773933 7 ACATGGG 11055 0.0 25.415049 3 CGCAGAG 7420 0.0 25.006145 8 CATGGGG 9660 0.0 23.828276 4 GAGTACA 7595 0.0 22.182386 1 GCAGAGT 8700 0.0 20.018 9 AGTACAT 7815 0.0 19.615902 2 ATGGGGG 6125 0.0 19.447412 5 AGAGTAC 6865 0.0 17.59224 10-11 TACACTG 3675 0.0 17.303806 5 CAGAGTA 7775 0.0 17.272688 10-11 >>END_MODULE