FastQCFastQC Report
Mon 15 Aug 2016
SRR2921420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214210
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.047.713151
GTATCAA48300.042.2206341
TCAACGC58800.034.7058144
CAACGCA60650.033.555445
ATCAACG61450.033.2091453
AACGCAG64050.031.8444676
TATCAAC63800.031.6139972
GTACATG102700.025.7809731
ACGCAGA80500.025.0414267
TACATGG105600.024.897572
ACATGGG109400.023.382053
CGCAGAG85600.023.3273078
CATGGGG93200.021.4885794
GCAGAGT95900.020.226969
ATGGGGG55300.019.6046775
AGAGTAC80000.018.60130510-11
GAGTACA79350.017.672161
CAGAGTA87700.016.9139110-11
TAAGGTG15450.015.6960975
AGTACAT82400.015.4354292
Produced by FastQC (version 0.11.5)