FastQCFastQC Report
Mon 15 Aug 2016
SRR2921419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763971
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28450.058.5503921
GTATCAA49100.041.5615651
TCAACGC62400.032.704874
CAACGCA63600.032.0114365
TATCAAC65050.031.2260592
ATCAACG66350.030.6852193
AACGCAG66950.030.6117956
GTACATG98350.029.483011
TACATGG102500.027.6421072
ACATGGG104050.026.5456713
ACGCAGA78000.025.9144137
CGCAGAG82050.024.2896168
CATGGGG89350.024.1682624
ATGGGGG58500.022.3902115
GCAGAGT90250.021.136299
GAGTACA71200.020.4627191
CAGAGTA82050.019.95218510-11
GTATAGG8100.019.9204391
GTGGTAT14050.019.5910021
AGAGTAC77000.018.5184110-11
Produced by FastQC (version 0.11.5)