##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921419_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2763971
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.261741892371518	31.0	31.0	34.0	26.0	34.0
2	30.303247392971922	31.0	31.0	34.0	26.0	34.0
3	30.352995744166634	33.0	31.0	34.0	26.0	34.0
4	33.811432536737904	37.0	35.0	37.0	29.0	37.0
5	33.80290495088407	37.0	35.0	37.0	29.0	37.0
6	33.85010153869197	37.0	35.0	37.0	30.0	37.0
7	33.766885397856925	37.0	35.0	37.0	29.0	37.0
8	33.73072076371279	37.0	35.0	37.0	29.0	37.0
9	35.27162875442615	39.0	35.0	39.0	29.0	39.0
10-11	35.162498629688955	39.0	35.0	39.0	28.0	39.0
12-13	35.039289124234664	39.0	35.0	39.0	27.0	39.0
14-15	36.14987874330085	40.0	36.0	41.0	27.0	41.0
16-17	36.09637402129039	40.0	36.0	41.0	27.0	41.0
18-19	36.04716583495268	40.0	36.0	41.0	27.0	41.0
20-21	35.978455996824856	40.0	36.0	41.0	27.0	41.0
22-23	35.86587467813519	40.0	36.0	41.0	27.0	41.0
24-25	35.76182836216444	40.0	36.0	41.0	27.0	41.0
26-27	35.60457779766864	40.0	36.0	41.0	26.0	41.0
28-29	35.461501405043684	40.0	36.0	41.0	25.0	41.0
30-31	35.30524198698177	39.5	35.0	41.0	25.0	41.0
32-33	35.114471895689206	39.0	35.0	41.0	24.0	41.0
34-35	34.94287005905633	39.0	35.0	41.0	24.0	41.0
36-37	34.767012027260776	39.0	35.0	41.0	23.0	41.0
38-39	34.99049573964416	39.0	35.0	41.0	24.0	41.0
40-41	35.099316526837654	40.0	35.0	41.0	24.0	41.0
42-43	34.924793349857865	40.0	35.0	41.0	22.5	41.0
44-45	34.78864557551436	40.0	35.0	41.0	21.5	41.0
46-47	34.619356534493306	39.0	35.0	41.0	19.5	41.0
48-49	34.458044603217616	39.0	35.0	41.0	18.0	41.0
50-51	32.74241517005786	37.0	32.0	39.5	15.0	40.0
52-53	32.891239633122055	37.5	32.5	39.5	13.0	40.0
54-55	33.72940200892123	38.0	34.0	40.0	13.0	41.0
56-57	33.731913974495384	38.0	34.0	41.0	12.5	41.0
58-59	33.543944925616074	38.0	34.0	40.5	9.0	41.0
60-61	33.29202133452196	37.5	33.0	40.0	8.0	41.0
62-63	32.99061694207356	37.0	33.0	40.0	6.5	41.0
64-65	32.697093240124445	36.5	33.0	40.0	2.0	41.0
66-67	32.358064719202915	36.0	33.0	39.0	2.0	41.0
68-69	31.99474867862217	35.5	32.0	39.0	2.0	41.0
70-71	31.61123958970626	35.0	32.0	38.5	2.0	40.0
72-73	31.262441791176535	35.0	32.0	37.0	2.0	40.0
74-75	30.881587759061148	35.0	31.5	37.0	2.0	39.0
76-77	30.519062971355346	35.0	31.0	36.0	2.0	39.0
78-79	30.185493444033966	35.0	31.0	36.0	2.0	38.0
80-81	29.855190412634578	35.0	31.0	35.5	2.0	37.0
82-83	29.565533972679162	35.0	31.0	35.0	2.0	37.0
84-85	29.34293250544235	35.0	30.0	35.0	2.0	36.0
86-87	29.12375310739512	35.0	30.0	35.0	2.0	36.0
88-89	28.925519659938544	34.5	30.0	35.0	2.0	36.0
90-91	28.6760575635562	34.0	30.0	35.0	2.0	35.0
92-93	28.485604950269014	34.0	30.0	35.0	2.0	35.0
94-95	28.28004400190885	34.0	29.0	35.0	2.0	35.0
96-97	28.0251010231294	34.0	29.0	35.0	2.0	35.0
98-99	27.796094097948206	34.0	29.0	35.0	2.0	35.0
100-101	26.750630885779916	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104712.0
3	17355.0
4	11834.0
5	9109.0
6	8202.0
7	8446.0
8	9029.0
9	9617.0
10	10431.0
11	10900.0
12	11101.0
13	11143.0
14	11975.0
15	13039.0
16	13515.0
17	14489.0
18	13115.0
19	12037.0
20	11914.0
21	12427.0
22	13593.0
23	15669.0
24	18391.0
25	21200.0
26	25476.0
27	29897.0
28	35653.0
29	42035.0
30	50239.0
31	61358.0
32	76533.0
33	98768.0
34	140411.0
35	203071.0
36	334257.0
37	587468.0
38	583636.0
39	101915.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70315768592983	17.71244146283105	12.751465035523196	24.832935815715924
2	16.309287725524864	19.45003754071738	39.022869790546686	25.21780494321107
3	19.670974591532634	23.445956193895352	29.069787224492437	27.813281990079574
4	13.7413992268309	15.292009077623527	35.800610846304856	35.16598084924071
5	14.539140973093737	37.21448451833804	33.683632502965516	14.562742005602711
6	33.93141207037554	34.380921019301894	16.947992733746826	14.739674176575743
7	29.465568031854666	31.814538613544673	20.37983015330638	18.340063201294278
8	27.43322929388881	34.18989460351925	19.634649658479216	18.742226444112724
9	27.630283800254183	15.445987337118108	18.932079590294684	37.99164927233302
10-11	26.32850298363223	24.87073098311039	27.125712433908916	21.675053599348466
12-13	26.72491477211039	22.358243038817594	27.152791349097775	23.76405083997423
14-15	23.86389775953075	23.8780867646806	24.44821691803587	27.809798557752778
16-17	24.017237775809388	27.02601318981187	25.27576188851904	23.680987145859703
18-19	24.109772660406303	26.337534935445195	26.64835771290311	22.904334691245392
20-21	24.506528204139943	25.417518260062373	26.821306075106694	23.25464746069099
22-23	24.457910829620946	25.187332653056053	26.424157107129915	23.930599410193082
24-25	24.2169386619371	25.42652508132711	26.354418810882223	24.00211744585356
26-27	23.797125823095367	25.7499715448633	26.383783702598674	24.069118929442663
28-29	23.957236977211714	26.16964405099414	26.095225274664628	23.777893697129514
30-31	24.41513135707907	25.64046617253073	26.021724467834122	23.922678002556076
32-33	24.096108063768824	25.746865209574914	25.95716288463135	24.199863842024907
34-35	24.422940748869905	25.872549626209107	26.040982577411313	23.66352704750967
36-37	24.44048912925499	25.664542398293445	26.1371842722614	23.757784200190162
38-39	24.277665793587992	25.78686532191565	26.043741494900285	23.89172738959607
40-41	24.424154876898267	25.578585842870826	26.09908742172441	23.898171858506498
42-43	24.183908721126596	25.648207520677502	25.880675900499977	24.287207857695922
44-45	24.004531861958366	25.73535368877143	26.144787225963633	24.115327223306572
46-47	24.277024564135722	25.66802736328824	25.838626492130633	24.21632158044541
48-49	24.066891455539672	25.905657911645758	25.815684361766895	24.211766271047676
50-51	24.203493443924682	25.885835495189724	25.96689038323266	23.94378067765294
52-53	24.436257583419884	25.616543138715635	25.992456003929693	23.95474327393479
54-55	23.735657945082895	25.571810735354706	26.32349473335437	24.36903658620803
56-57	23.932815688714236	25.777962435054235	25.971145608667484	24.31807626756404
58-59	24.032968403437142	25.68956378804918	26.09846944065593	24.178998367857744
60-61	24.100029004423174	25.676890725835687	26.308135740700457	23.914944529040678
62-63	24.075380582756583	25.65526580066491	26.339444651901374	23.929908964677136
64-65	23.79414308948513	25.521363939257046	26.284602606148983	24.399890365108842
66-67	23.83992764189991	25.647629465624917	26.08730642803478	24.425136464440396
68-69	23.95550501385144	25.568902303304842	26.274107704097577	24.201484978746144
70-71	23.86109427736346	25.411488477017823	26.199525336642736	24.52789190897598
72-73	23.80711161294006	25.764631148172345	25.995637592103083	24.432619646784516
74-75	23.80531821902969	25.84553534970555	26.11344173434858	24.235704696916176
76-77	23.854534026836234	25.358352020202663	26.130269393581262	24.65684455937984
78-79	23.773031825795645	25.42974613326372	26.240654934122272	24.556567106818363
80-81	24.045321647763913	25.6644699115986	25.907514381702374	24.38269405893511
82-83	23.976752479131854	25.609927441237158	26.11916040466698	24.294159674964007
84-85	23.88094524606016	25.753337789338982	26.01223207994125	24.35348488465961
86-87	23.782334239004797	26.000168623515364	25.889747418292387	24.32774971918745
88-89	23.64574248772961	25.68352458882638	26.02535487579121	24.64537804765281
90-91	23.842605435509036	25.416590969006204	26.14223690122244	24.598566694262317
92-93	24.038643902336425	25.29300051603049	26.22073122571928	24.447624355913806
94-95	23.949192012571796	25.255949425575828	26.244440632318117	24.550417929534255
96-97	23.87085133487168	24.94045325342388	26.37905912967001	24.80963628203443
98-99	23.608231645955673	25.104848392759816	26.273058033698714	25.013861927585797
100-101	23.921994803981498	24.99679487586332	26.38938731264077	24.691823007514408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	90.5
2	96.0
3	154.0
4	168.0
5	168.5
6	252.5
7	477.5
8	780.5
9	907.0
10	784.5
11	663.0
12	653.5
13	710.5
14	791.5
15	888.5
16	1021.0
17	1122.0
18	1270.5
19	1393.0
20	1477.5
21	1651.5
22	1882.5
23	2134.0
24	2471.0
25	3025.0
26	3897.0
27	5220.5
28	7087.5
29	9833.0
30	13724.0
31	18979.5
32	26409.0
33	31005.0
34	33122.5
35	37867.0
36	45033.0
37	52378.5
38	58792.5
39	64532.5
40	69249.5
41	74290.0
42	79320.0
43	85431.5
44	91562.5
45	98875.5
46	105331.0
47	110058.0
48	115210.5
49	118202.5
50	119413.5
51	120080.5
52	120875.5
53	121166.0
54	119141.0
55	114299.0
56	107003.0
57	97392.0
58	88447.0
59	77533.0
60	64101.5
61	53374.5
62	44785.5
63	34853.0
64	25113.0
65	18421.5
66	13889.5
67	10692.0
68	8119.5
69	6230.0
70	4837.0
71	3471.5
72	2474.0
73	1931.0
74	1497.0
75	1144.0
76	876.5
77	597.5
78	379.5
79	266.0
80	202.5
81	144.5
82	106.5
83	96.0
84	75.5
85	67.0
86	61.0
87	46.5
88	45.5
89	38.5
90	28.0
91	28.5
92	24.0
93	18.0
94	16.5
95	15.5
96	16.0
97	13.5
98	11.0
99	6.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.051013559838363
2	0.059226381174042705
3	0.05021760358556584
4	0.05694705190466905
5	0.049928164948185055
6	0.01848789296269751
7	0.015195528462491104
8	0.02156317848486833
9	0.02239531456733808
10-11	0.023317900223989326
12-13	0.02861824527102491
14-15	0.045894113939690395
16-17	0.03087948462556228
18-19	0.023878687583914593
20-21	0.027804199103391464
22-23	0.04963872631080428
24-25	0.043723324159334526
26-27	0.030734765306871886
28-29	0.020097895383128114
30-31	0.012988558852462636
32-33	0.009750464096765126
34-35	0.009406755714875445
36-37	0.0014110133572313168
38-39	0.006801807978448399
40-41	0.0035637132227508903
42-43	0.21568605459319218
44-45	0.19108377041582564
46-47	0.19745142043820288
48-49	0.194737933212758
50-51	0.1934354593445445
52-53	0.1984825455838719
54-55	0.19651074486671533
56-57	0.20651446777118862
58-59	0.2038009805457438
60-61	0.20879379704056228
62-63	0.20626120896348044
64-65	0.20712952487562278
66-67	0.19784939856460146
68-69	0.19584141801777225
70-71	0.1795604946651032
72-73	0.18717634881118506
74-75	0.18710398915183987
76-77	0.18547589681657295
78-79	0.21096458682091815
80-81	0.2185080813076548
82-83	0.2170608881207509
84-85	0.21329818583480073
86-87	0.22968764867648755
88-89	0.22851180421212813
90-91	0.22757112864064058
92-93	0.23089967297051958
94-95	0.24197070085033454
96-97	0.24180789161680785
98-99	0.2322021468387331
100-101	0.24122901434204627
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2763971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.75184112489768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25126010972978	43.068999803358004
2	12.058674598705213	13.445866208077048
3	4.098159016890939	6.854397312428115
4	1.934589652575968	4.314277398091455
5	1.114595589825824	3.107037812124048
6	0.7275144255518737	2.4336161201663558
7	0.5106870191911499	1.9930219090944796
8	0.35926809571175755	1.6023886234693152
9	0.28257112452637484	1.4178474396970315
>10	1.5668550869720794	15.458121012752413
>50	0.07042004221404492	2.664850494214478
>100	0.022927313661077874	2.204136605951296
>500	0.001304170760041463	0.5143499812405813
>1k	0.0011737536839552835	0.9210892793355137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.617982967259787E-5
2	0.0	0.0	0.0	0.0	3.617982967259787E-5
3	0.0	0.0	0.0	0.0	3.617982967259787E-5
4	0.0	0.0	0.0	3.617982967259787E-5	3.617982967259787E-5
5	0.0	0.0	0.0	3.617982967259787E-5	3.617982967259787E-5
6	0.0	0.0	0.0	3.617982967259787E-5	7.235965934519574E-5
7	0.0	0.0	0.0	3.617982967259787E-5	7.235965934519574E-5
8	0.0	0.0	0.0	3.617982967259787E-5	7.235965934519574E-5
9	0.0	0.0	0.0	7.235965934519574E-5	7.235965934519574E-5
10-11	0.0	0.0	0.0	1.2662940385409254E-4	7.235965934519574E-5
12-13	0.0	0.0	0.0	1.4471931869039148E-4	7.235965934519574E-5
14-15	0.0	0.0	0.0	1.4471931869039148E-4	9.044957418149467E-5
16-17	0.0	0.0	0.0	1.8089914836298934E-4	1.4471931869039148E-4
18-19	0.0	0.0	0.0	1.8089914836298934E-4	1.4471931869039148E-4
20-21	0.0	0.0	0.0	1.9898906319928825E-4	1.4471931869039148E-4
22-23	0.0	0.0	0.0	2.71348722544484E-4	2.3516889287188614E-4
24-25	0.0	0.0	0.0	5.065176154163702E-4	3.256184670533808E-4
26-27	0.0	0.0	0.0	0.0010311251456690393	3.256184670533808E-4
28-29	0.0	0.0	0.0	0.0035998930524234877	3.256184670533808E-4
30-31	0.0	0.0	0.0	0.013277997489843417	3.4370838188967973E-4
32-33	0.0	0.0	0.0	0.03498589529340214	4.341579560711744E-4
34-35	0.0	0.0	0.0	0.06313380277868327	4.341579560711744E-4
36-37	0.0	0.0	0.0	0.10658577821547331	4.7033778574377227E-4
38-39	0.0	0.0	0.0	0.17346419336527047	5.246075302526691E-4
40-41	0.0	0.0	0.0	0.25002071295248757	5.42697445088968E-4
42-43	0.0	0.0	0.0	0.3306112835481993	5.42697445088968E-4
44-45	0.0	0.0	0.0	0.4310826705490036	5.60787359925267E-4
46-47	0.0	0.0	0.0	0.5487575665591282	5.788772747615659E-4
48-49	0.0	0.0	0.0	0.6676263969484484	5.788772747615659E-4
50-51	0.0	0.0	0.0	0.7949974873108292	5.788772747615659E-4
52-53	0.0	0.0	0.0	0.925878021151452	6.150571044341638E-4
54-55	0.0	0.0	0.0	1.0825005038041282	6.512369341067616E-4
56-57	0.0	0.0	0.0	1.257918407971719	6.512369341067616E-4
58-59	0.0	0.0	0.0	1.4427611577690214	6.512369341067616E-4
60-61	0.0	0.0	0.0	1.6451692148723702	6.693268489430605E-4
62-63	0.0	0.0	0.0	1.8585759401961888	7.597764231245552E-4
64-65	0.0	0.0	0.0	2.0873048233863525	7.597764231245552E-4
66-67	0.0	0.0	0.0	2.359286692950107	7.597764231245552E-4
68-69	0.0	0.0	0.0	2.651981515001424	7.778663379608542E-4
70-71	0.0	0.0	0.0	2.957411637097495	8.683159121423488E-4
72-73	0.0	0.0	0.0	3.280895494200192	0.0012843839533772242
74-75	0.0	0.0	0.0	3.6277153414417156	0.0013024738682135232
76-77	0.0	0.0	0.0	4.018240422927737	0.0013024738682135232
78-79	0.0	0.0	0.0	4.451602422746114	0.0013024738682135232
80-81	0.0	0.0	0.0	4.898495678862043	0.001338653697886121
82-83	0.0	0.0	0.0	5.362809522965328	0.001374833527558719
84-85	0.0	0.0	0.0	5.856646108081453	0.001374833527558719
86-87	0.0	0.0	0.0	6.38360532726284	0.001374833527558719
88-89	0.0	0.0	0.0	6.949548312916452	0.0014291032720676158
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2845	0.0	58.550392	1
GTATCAA	4910	0.0	41.561565	1
TCAACGC	6240	0.0	32.70487	4
CAACGCA	6360	0.0	32.011436	5
TATCAAC	6505	0.0	31.226059	2
ATCAACG	6635	0.0	30.685219	3
AACGCAG	6695	0.0	30.611795	6
GTACATG	9835	0.0	29.48301	1
TACATGG	10250	0.0	27.642107	2
ACATGGG	10405	0.0	26.545671	3
ACGCAGA	7800	0.0	25.914413	7
CGCAGAG	8205	0.0	24.289616	8
CATGGGG	8935	0.0	24.168262	4
ATGGGGG	5850	0.0	22.390211	5
GCAGAGT	9025	0.0	21.13629	9
GAGTACA	7120	0.0	20.462719	1
CAGAGTA	8205	0.0	19.952185	10-11
GTATAGG	810	0.0	19.920439	1
GTGGTAT	1405	0.0	19.591002	1
AGAGTAC	7700	0.0	18.51841	10-11
>>END_MODULE