FastQCFastQC Report
Mon 15 Aug 2016
SRR2921419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763971
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.057.1040881
GTATCAA51300.045.1203421
TCAACGC68700.033.437064
ATCAACG70350.032.787753
TATCAAC70150.032.611192
CAACGCA71000.032.2756845
AACGCAG75450.030.3063226
GTACATG98000.026.044081
TACATGG100150.025.0224762
ACGCAGA92150.024.4013167
ACATGGG105350.023.2913933
CGCAGAG95200.023.0702558
TATACCG2103.4176992E-822.6395345
CATGGGG87550.020.4373514
GCAGAGT102850.020.2911959
GTATATA7500.020.2791391
AGAGTAC88550.018.92421710-11
ATGGGGG56850.018.5656135
GTGGTAT14250.018.0110781
GAGTACA76700.017.84671
Produced by FastQC (version 0.11.5)