##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921419_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2763971
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.346480480439194	33.0	31.0	34.0	28.0	34.0
2	31.473516545578807	34.0	31.0	34.0	28.0	34.0
3	31.54561824273844	34.0	31.0	34.0	28.0	34.0
4	35.035195376507204	37.0	35.0	37.0	32.0	37.0
5	34.77466876461439	37.0	35.0	37.0	32.0	37.0
6	34.72697434235019	37.0	35.0	37.0	32.0	37.0
7	34.59471282441096	37.0	35.0	37.0	32.0	37.0
8	34.553753639238614	37.0	35.0	37.0	32.0	37.0
9	36.168857053854765	39.0	37.0	39.0	32.0	39.0
10-11	36.01810547216306	39.0	37.0	39.0	31.0	39.0
12-13	35.94180999004693	39.0	37.0	39.0	31.0	39.0
14-15	37.17128381593005	40.0	38.0	41.0	31.0	41.0
16-17	37.14527467907587	40.0	38.0	41.0	31.0	41.0
18-19	37.11084649585686	40.0	38.0	41.0	31.0	41.0
20-21	37.06954414500007	40.0	38.0	41.0	31.0	41.0
22-23	36.96181544596524	40.0	37.5	41.0	30.5	41.0
24-25	36.88691614347618	40.0	37.0	41.0	30.0	41.0
26-27	36.74272667839134	40.0	37.0	41.0	30.0	41.0
28-29	36.58563982762482	40.0	37.0	41.0	30.0	41.0
30-31	36.43866433475605	40.0	37.0	41.0	30.0	41.0
32-33	36.37547969931667	40.0	36.5	41.0	30.0	41.0
34-35	36.495082437550906	40.0	37.0	41.0	30.0	41.0
36-37	36.464414243130626	40.0	37.0	41.0	30.0	41.0
38-39	36.368942908590576	40.0	37.0	41.0	29.5	41.0
40-41	36.232652947516456	40.0	36.0	41.0	29.0	41.0
42-43	36.112073172981916	40.0	36.0	41.0	28.5	41.0
44-45	35.95661929882766	40.0	36.0	41.0	28.0	41.0
46-47	35.78177538765783	40.0	35.0	41.0	27.0	41.0
48-49	35.60794632071031	40.0	35.0	41.0	26.5	41.0
50-51	35.44060429722309	39.5	35.0	41.0	26.0	41.0
52-53	35.25656347335048	39.0	35.0	41.0	26.0	41.0
54-55	35.03551846238618	39.0	35.0	41.0	26.0	41.0
56-57	34.764674991163076	39.0	35.0	41.0	25.0	41.0
58-59	34.52739916591021	38.0	34.5	40.0	24.0	41.0
60-61	34.242356196935496	38.0	34.0	40.0	23.5	41.0
62-63	33.93397940861174	37.0	34.0	40.0	23.0	41.0
64-65	33.61468210049961	37.0	34.0	40.0	22.5	41.0
66-67	33.27919594670132	36.0	34.0	39.0	22.0	41.0
68-69	32.9171263012528	35.5	33.0	39.0	21.5	40.5
70-71	32.54023341778911	35.0	33.0	38.5	20.0	40.0
72-73	32.1544811794335	35.0	33.0	37.0	18.5	39.5
74-75	31.761563706710383	35.0	32.5	37.0	17.5	39.0
76-77	29.04608206815484	33.0	28.5	34.5	9.0	36.5
78-79	30.699541710097538	34.5	31.5	35.5	11.5	37.0
80-81	30.906951990451418	35.0	32.0	35.0	11.5	37.0
82-83	30.753847996234406	35.0	32.0	35.0	8.5	37.0
84-85	30.53503256727368	35.0	32.0	35.0	7.0	36.0
86-87	30.304998858526375	35.0	32.0	35.0	2.0	36.0
88-89	30.033354004076017	35.0	31.0	35.0	2.0	36.0
90-91	29.85493425944049	34.0	31.0	35.0	2.0	35.0
92-93	29.71267969164655	34.0	31.0	35.0	2.0	35.0
94-95	29.619476289729526	34.0	31.0	35.0	2.0	35.0
96-97	29.430845692664647	34.0	31.0	35.0	2.0	35.0
98-99	29.16670145960287	34.0	31.0	35.0	2.0	35.0
100-101	28.09191214379601	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56209.0
3	18419.0
4	10045.0
5	7057.0
6	6296.0
7	6265.0
8	6570.0
9	7054.0
10	7675.0
11	8322.0
12	8849.0
13	9833.0
14	10516.0
15	11885.0
16	13064.0
17	14588.0
18	13244.0
19	11183.0
20	10580.0
21	10198.0
22	11052.0
23	12140.0
24	14027.0
25	17010.0
26	20524.0
27	24707.0
28	29491.0
29	35781.0
30	43453.0
31	53437.0
32	68420.0
33	91215.0
34	133622.0
35	199433.0
36	348706.0
37	666170.0
38	662790.0
39	84136.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.512686827562995	19.19497969259712	13.386218214086298	25.90611526575359
2	15.224034281347867	20.730569850706523	37.64866394206556	26.396731925880047
3	18.109210297320306	24.60073804529799	29.054301217857958	28.235750439523738
4	13.311228877720078	16.494436920712328	34.83072688177023	35.36360731979737
5	14.217144519816213	37.535791939177585	33.79204195727261	14.455021583733597
6	33.98421529829497	34.93977080561025	16.651290077722326	14.424723818372456
7	29.259073000068742	32.01333589004222	20.423050901420783	18.304540208468254
8	27.345315086335553	34.790741427498936	19.289126875729263	18.574816610436244
9	27.2975367686564	15.495459250476939	18.916117426702378	38.29088655416428
10-11	26.153819408667157	25.223719591619503	27.09286493089805	21.52959606881529
12-13	26.580178674315093	22.52381766428151	27.291782555330336	23.604221106073066
14-15	23.721427268885428	24.061969937304255	24.47959783256821	27.737004961242107
16-17	23.901990129041	27.257331085597457	25.306891382366608	23.533787402994935
18-19	23.918323783336422	26.524744818354083	26.72718967786285	22.82974172044664
20-21	24.35226365141948	25.52147694289247	26.90925404832391	23.217005357364137
22-23	24.28951730954722	25.228602667326587	26.631224841405434	23.85065518172076
24-25	24.043317368120565	25.56195426360908	26.485965977381447	23.908762390888903
26-27	23.663561748908695	25.881609105989916	26.564413626422134	23.890415518679255
28-29	23.817877559881325	26.227078659816193	26.291844561835156	23.663199218467327
30-31	24.287067866395255	25.744331741973557	26.177883349776437	23.790717041854748
32-33	23.98109766897907	25.810630399144298	26.063997912848013	24.144274019028618
34-35	24.228620597448376	25.95463323362231	26.23788152854211	23.5788646403872
36-37	24.258792435832383	25.788974304008484	26.319138986650458	23.633094273508682
38-39	24.207967825942866	25.863125667971314	26.194207888230174	23.734698617855646
40-41	24.25405772012925	25.699258982939117	26.2044806196025	23.84220267732914
42-43	24.04129582708797	25.730180454406064	25.95220025201563	24.276323466490336
44-45	23.928724042844888	25.757696915444562	26.23042641177507	24.083152629935483
46-47	24.171159719666782	25.77729726443901	25.886516354278722	24.16502666161549
48-49	23.930711447862617	26.00265589833771	25.930288096220227	24.13634455757944
50-51	24.108323125554147	25.93441657906158	26.00790173468023	23.94935856070404
52-53	24.385440296968447	25.72141841508287	25.884067252064995	24.00907403588369
54-55	23.604814499578048	25.684181201309364	26.30279030768549	24.4082139914271
56-57	23.828887225319605	25.879286620645537	25.92755344718853	24.364272706846325
58-59	23.93408717481676	25.807654997212083	26.0191098128567	24.23914801511446
60-61	23.993525477647086	25.85517679563608	26.185271058660746	23.96602666805608
62-63	23.950523645797126	25.809695557546213	26.221045598066052	24.01873519859061
64-65	23.60372254997605	25.64089339896493	26.24116516892789	24.514218882131132
66-67	23.648593747031	25.862441196882653	26.017077900162057	24.47188715592429
68-69	23.754328994341524	25.760264504969694	26.094623280005656	24.390783220683122
70-71	23.710571721128275	25.589874596112033	26.003218435569575	24.696335247190113
72-73	23.60767284742879	25.9937619979846	25.78331945160446	24.61524570298215
74-75	23.564211822646726	26.080764255466676	25.842386785629824	24.51263713625677
76-77	23.58209680531157	25.57408070929249	25.985184873571686	24.85863761182425
78-79	23.454372231586163	25.682770570732462	26.043514048413268	24.819343149268114
80-81	23.77884504872855	25.956928467808567	25.645272077169302	24.618954406293582
82-83	23.67062506966046	25.901967283828203	25.823495225453826	24.603912421057508
84-85	23.54207774234223	26.00532884365106	25.698840712307586	24.75375270169912
86-87	23.384015721588128	26.267942280553587	25.552342080322397	24.795699917535888
88-89	23.23647960289493	25.982410819401025	25.708555885432542	25.072553692271505
90-91	23.28268226255564	25.790598042432357	25.825186479949597	25.10153321506241
92-93	23.445733990019395	25.70351464114057	25.86233483604688	24.98841653279316
94-95	23.315194846568808	25.701323363587864	25.87009288022032	25.11338890962301
96-97	23.107749989687715	25.48813291196493	25.98567352246667	25.41844357588069
98-99	22.827780544559282	25.586395370524308	25.866520691555884	25.719303393360534
100-101	22.859975780853993	25.6582929590449	25.915589727521944	25.566141532579167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	71.5
2	81.0
3	118.5
4	117.0
5	134.0
6	233.0
7	457.5
8	699.5
9	782.5
10	661.5
11	521.5
12	487.5
13	503.5
14	602.5
15	692.5
16	715.0
17	806.5
18	926.5
19	1042.5
20	1204.5
21	1360.5
22	1560.0
23	1893.5
24	2372.5
25	2989.0
26	3995.5
27	5543.5
28	7699.5
29	10860.0
30	14925.5
31	20080.5
32	27599.0
33	32137.5
34	33930.5
35	38449.0
36	45469.0
37	52300.5
38	58497.0
39	64782.0
40	69910.0
41	74664.5
42	79813.0
43	85889.5
44	92223.5
45	99631.5
46	106000.5
47	111196.5
48	116533.0
49	119882.5
50	120908.5
51	121385.0
52	121472.0
53	121432.0
54	120371.0
55	114755.0
56	106380.5
57	96665.0
58	87143.0
59	76259.5
60	62915.5
61	52162.0
62	43506.5
63	33483.0
64	23821.5
65	17177.5
66	12834.5
67	9802.0
68	7531.0
69	5752.0
70	4300.5
71	3105.5
72	2197.0
73	1651.5
74	1263.5
75	925.0
76	665.0
77	408.0
78	230.5
79	145.0
80	90.5
81	51.0
82	24.5
83	13.5
84	13.5
85	9.5
86	8.5
87	6.0
88	3.0
89	3.5
90	2.5
91	1.0
92	1.5
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14978449484455517
2	6.874167637793595E-4
3	0.006657088659758008
4	0.004486298879402136
5	0.028184087314953737
6	0.0010492150605053382
7	3.617982967259787E-5
8	0.001664272164939502
9	0.0
10-11	0.10381802124551959
12-13	1.2662940385409254E-4
14-15	0.005318434961871887
16-17	1.628092335266904E-4
18-19	3.075285522170819E-4
20-21	6.693268489430605E-4
22-23	1.8089914836298934E-4
24-25	0.0016280923352669041
26-27	0.002749667055117438
28-29	0.006186750874014235
30-31	0.014526201613548043
32-33	0.014345302465185053
34-35	0.011505185835886122
36-37	6.693268489430605E-4
38-39	1.8089914836298934E-4
40-41	0.004866187090964413
42-43	0.00917158682200356
44-45	0.008466080143387901
46-47	0.009406755714875445
48-49	0.011251927028177936
50-51	0.005336524876708186
52-53	0.0014833730165765126
54-55	0.0010311251456690393
56-57	0.006078211384996442
58-59	0.009406755714875445
60-61	0.0077786633796085415
62-63	0.018433623218188613
64-65	0.03518488435660143
66-67	0.03775365226335588
68-69	0.027912738592409253
70-71	0.04117264616741637
72-73	0.02700824285059431
74-75	0.025561049663690393
76-77	0.2358563096356655
78-79	0.06865122680375445
80-81	0.05331097902257296
82-83	0.020513963424362992
84-85	0.03238094755697509
86-87	0.0782750614966655
88-89	0.2612002803213203
90-91	0.3153072155966904
92-93	0.051122099327380786
94-95	0.005155625728345196
96-97	0.01002181281930961
98-99	0.0010673049753416371
100-101	0.0015195528462491105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2763971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.75289987855756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56479861873069	41.921447529932834
2	12.306156574083232	13.475955175812643
3	4.258366406234843	6.99473728460368
4	2.0375666859884225	4.462507390152337
5	1.139482581475847	3.119498784845368
6	0.739613994930713	2.4297606607932782
7	0.5207696026954786	1.9959552141327213
8	0.372426260389351	1.6313134197794996
9	0.30960748331393234	1.5256716781985926
>10	1.6499441765530267	15.97756056468283
>50	0.07440728275652052	2.753071336441057
>100	0.02440400489173638	2.3060714183801907
>500	0.0013277448412244586	0.5218253851858954
>1k	0.0011285831149759605	0.8846241570591514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	3.617982967259787E-5
10-11	0.0	0.0	0.0	7.235965934519574E-5	3.617982967259787E-5
12-13	0.0	0.0	0.0	1.085394890177936E-4	3.617982967259787E-5
14-15	0.0	0.0	0.0	1.085394890177936E-4	3.617982967259787E-5
16-17	0.0	0.0	0.0	1.085394890177936E-4	3.617982967259787E-5
18-19	0.0	0.0	0.0	1.085394890177936E-4	7.235965934519574E-5
20-21	0.0	0.0	0.0	1.2662940385409254E-4	1.4471931869039148E-4
22-23	0.0	0.0	0.0	2.3516889287188614E-4	2.1707897803558722E-4
24-25	0.0	0.0	0.0	5.065176154163702E-4	2.8943863738078296E-4
26-27	0.0	0.0	0.0	0.0010673049753416371	2.8943863738078296E-4
28-29	0.0	0.0	0.0	0.003744612371113879	2.8943863738078296E-4
30-31	0.0	0.0	0.0	0.013657885701405695	3.075285522170819E-4
32-33	0.0	0.0	0.0	0.03590848095005338	3.256184670533808E-4
34-35	0.0	0.0	0.0	0.06427346741337012	3.9797812639857656E-4
36-37	0.0	0.0	0.0	0.1085394890177936	3.9797812639857656E-4
38-39	0.0	0.0	0.0	0.1764671192280961	4.1606804123487545E-4
40-41	0.0	0.0	0.0	0.25385577489778294	4.7033778574377227E-4
42-43	0.0	0.0	0.0	0.33544129080949114	4.884277005800712E-4
44-45	0.0	0.0	0.0	0.43650964499989325	5.246075302526691E-4
46-47	0.0	0.0	0.0	0.5550347670073239	6.150571044341638E-4
48-49	0.0	0.0	0.0	0.6738674175669715	6.874167637793595E-4
50-51	0.0	0.0	0.0	0.8006777205694271	7.235965934519574E-4
52-53	0.0	0.0	0.0	0.9321371316848115	8.14046167633452E-4
54-55	0.0	0.0	0.0	1.0887053445929786	9.044957418149467E-4
56-57	0.0	0.0	0.0	1.2633996521671176	9.044957418149467E-4
58-59	0.0	0.0	0.0	1.4493277968545981	9.044957418149467E-4
60-61	0.0	0.0	0.0	1.6521338320843455	9.044957418149467E-4
62-63	0.0	0.0	0.0	1.8659566254493987	9.044957418149467E-4
64-65	0.0	0.0	0.0	2.096675399271555	9.044957418149467E-4
66-67	3.617982967259787E-5	0.0	0.0	2.3706471594673024	9.225856566512456E-4
68-69	3.617982967259787E-5	0.0	0.0	2.6639389487082172	9.406755714875445E-4
70-71	3.617982967259787E-5	0.0	0.0	2.970327836290612	9.949453159964414E-4
72-73	3.617982967259787E-5	0.0	0.0	3.296669899937445	0.0011939343791957295
74-75	3.617982967259787E-5	0.0	0.0	3.64636604363794	0.0011939343791957295
76-77	3.617982967259787E-5	0.0	0.0	4.041196524855	0.0011939343791957295
78-79	3.617982967259787E-5	0.0	0.0	4.479207632786307	0.0011939343791957295
80-81	3.617982967259787E-5	0.0	0.0	4.933427304410936	0.0012301142088683274
82-83	3.617982967259787E-5	0.0	0.0	5.404090708621762	0.0012843839533772242
84-85	3.617982967259787E-5	0.0	0.0	5.904964270609207	0.0013024738682135232
86-87	3.617982967259787E-5	0.0	0.0	6.438526308705844	0.001338653697886121
88-89	3.617982967259787E-5	0.0	0.0	7.0141835786265485	0.001374833527558719
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2755	0.0	57.104088	1
GTATCAA	5130	0.0	45.120342	1
TCAACGC	6870	0.0	33.43706	4
ATCAACG	7035	0.0	32.78775	3
TATCAAC	7015	0.0	32.61119	2
CAACGCA	7100	0.0	32.275684	5
AACGCAG	7545	0.0	30.306322	6
GTACATG	9800	0.0	26.04408	1
TACATGG	10015	0.0	25.022476	2
ACGCAGA	9215	0.0	24.401316	7
ACATGGG	10535	0.0	23.291393	3
CGCAGAG	9520	0.0	23.070255	8
TATACCG	210	3.4176992E-8	22.639534	5
CATGGGG	8755	0.0	20.437351	4
GCAGAGT	10285	0.0	20.291195	9
GTATATA	750	0.0	20.279139	1
AGAGTAC	8855	0.0	18.924217	10-11
ATGGGGG	5685	0.0	18.565613	5
GTGGTAT	1425	0.0	18.011078	1
GAGTACA	7670	0.0	17.8467	1
>>END_MODULE