FastQCFastQC Report
Mon 15 Aug 2016
SRR2921418_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921418_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581885
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.054.1297761
GTATCAA61050.045.6304931
TCAACGC75750.036.4652754
CAACGCA77750.035.401715
ATCAACG81350.033.89533
TATCAAC81700.033.8676952
AACGCAG83700.032.8756
GTACATG137050.029.8490811
TACATGG145650.027.8934612
ACGCAGA99150.027.1328897
GTATAGG12700.026.9049071
ACATGGG151500.026.3140453
CGCAGAG102950.025.6723048
CATGGGG121800.024.8216884
GCAGAGT115400.021.5457449
GAGTACA101900.021.3301541
GTATATG9800.020.8230881
GTATACG1856.6097364E-620.522061
AGTACAT101150.020.1293872
ATGGGGG72950.020.036515
Produced by FastQC (version 0.11.5)