##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921418_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3581885
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.460094335803635	31.0	31.0	34.0	26.0	34.0
2	30.503023687248476	33.0	31.0	34.0	26.0	34.0
3	30.557976875304483	33.0	31.0	34.0	26.0	34.0
4	34.01992889218945	37.0	35.0	37.0	30.0	37.0
5	34.011103650731386	37.0	35.0	37.0	30.0	37.0
6	34.05493392445598	37.0	35.0	37.0	30.0	37.0
7	33.97185923054481	37.0	35.0	37.0	30.0	37.0
8	33.939363770751996	37.0	35.0	37.0	30.0	37.0
9	35.49753188614375	39.0	37.0	39.0	30.0	39.0
10-11	35.38468306492252	39.0	37.0	39.0	30.0	39.0
12-13	35.26942838756688	39.0	35.0	39.0	29.0	39.0
14-15	36.40700776267245	40.0	37.0	41.0	29.0	41.0
16-17	36.348989707933114	40.0	37.0	41.0	28.0	41.0
18-19	36.2919906697172	40.0	37.0	41.0	27.5	41.0
20-21	36.228521714125385	40.0	37.0	41.0	27.0	41.0
22-23	36.10864447071863	40.0	36.0	41.0	27.0	41.0
24-25	36.0080888973264	40.0	36.0	41.0	27.0	41.0
26-27	35.85358952060159	40.0	36.0	41.0	27.0	41.0
28-29	35.71158593868871	40.0	36.0	41.0	26.5	41.0
30-31	35.56513832800327	40.0	36.0	41.0	26.0	41.0
32-33	35.383465409972686	39.5	35.0	41.0	25.0	41.0
34-35	35.22089053668669	39.0	35.0	41.0	25.0	41.0
36-37	35.05290803585263	39.0	35.0	41.0	24.0	41.0
38-39	35.29584715868879	39.0	35.0	41.0	25.0	41.0
40-41	35.42288264419433	40.0	35.0	41.0	25.0	41.0
42-43	35.25066480358805	40.0	35.0	41.0	24.0	41.0
44-45	35.128002713654965	40.0	35.0	41.0	24.0	41.0
46-47	34.9681179043995	40.0	35.0	41.0	23.0	41.0
48-49	34.81583705227834	39.0	35.0	41.0	22.5	41.0
50-51	33.08573083725469	37.0	32.5	39.5	18.5	40.0
52-53	33.241125971380995	37.5	33.0	39.5	19.0	40.0
54-55	34.096575825298686	38.5	34.0	40.5	20.0	41.0
56-57	34.09928180273794	38.5	34.0	41.0	20.0	41.0
58-59	33.90027359895697	38.0	34.0	40.5	18.5	41.0
60-61	33.64150147199031	37.5	34.0	40.0	18.0	41.0
62-63	33.338762411411864	37.0	33.5	40.0	15.0	41.0
64-65	33.0392421867257	36.5	33.0	40.0	13.0	41.0
66-67	32.68962417833068	36.0	33.0	39.0	10.0	41.0
68-69	32.31814561327346	35.5	33.0	39.0	7.5	41.0
70-71	31.941038308041712	35.0	32.0	38.5	6.0	40.0
72-73	31.584479819983052	35.0	32.0	37.0	2.0	39.5
74-75	31.19528125554003	35.0	32.0	37.0	2.0	39.0
76-77	30.836642019495322	35.0	32.0	36.0	2.0	39.0
78-79	30.50978102312051	35.0	31.0	36.0	2.0	37.5
80-81	30.17861544968641	35.0	31.0	35.5	2.0	37.0
82-83	29.902277990499414	35.0	31.0	35.0	2.0	37.0
84-85	29.678667238060406	35.0	31.0	35.0	2.0	36.0
86-87	29.46560205031708	35.0	31.0	35.0	2.0	36.0
88-89	29.2809932479686	35.0	31.0	35.0	2.0	36.0
90-91	29.036524344025562	34.5	30.0	35.0	2.0	35.0
92-93	28.846680448981473	34.0	30.0	35.0	2.0	35.0
94-95	28.64524656710084	34.0	30.0	35.0	2.0	35.0
96-97	28.39532034110531	34.0	30.0	35.0	2.0	35.0
98-99	28.17164635380533	34.0	30.0	35.0	2.0	35.0
100-101	27.105687787296354	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121039.0
3	21176.0
4	14814.0
5	11151.0
6	10361.0
7	10645.0
8	11007.0
9	11644.0
10	12729.0
11	13217.0
12	13259.0
13	13581.0
14	14189.0
15	15330.0
16	15516.0
17	16671.0
18	15602.0
19	14263.0
20	14397.0
21	15308.0
22	16970.0
23	19402.0
24	22734.0
25	27003.0
26	32013.0
27	37765.0
28	45284.0
29	53506.0
30	63924.0
31	78544.0
32	98060.0
33	127933.0
34	181610.0
35	263393.0
36	437661.0
37	785313.0
38	776928.0
39	127935.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.76776762964382	17.654391887551053	12.551598037808114	25.02624244499701
2	16.361360458171614	19.26473981463738	38.908020964334824	25.465878762856185
3	19.772359522127616	23.465784237898237	28.641059137978374	28.12079710199577
4	13.822276004165493	14.960831176841285	35.507730968119674	35.70916185087354
5	14.804760449655127	37.05283498027745	33.47339201590094	14.669012554166482
6	34.47109735557055	33.91185346849604	16.9052044194452	14.711844756488212
7	30.177898103968975	31.24916233313676	20.226778757661858	18.346160805232405
8	27.69498448568193	34.27541768029112	19.371982861648917	18.65761497237804
9	27.916068169481	15.308645836358398	18.755542847186078	38.01974314697453
10-11	26.635108661915556	24.790732982224466	26.816112956509127	21.75804539935085
12-13	26.83732822630438	22.086001272847657	26.99093284541771	24.085737655430254
14-15	24.19177076705737	23.58253001515491	24.147752822483113	28.077946395304604
16-17	24.450734039493195	26.7133545758433	24.85513297047924	23.98077841418426
18-19	24.49039914417047	26.219106098232185	26.069612383055755	23.220882374541585
20-21	24.991011442102586	25.318174005355853	26.098876510548973	23.591938041992588
22-23	24.987675569025612	24.879779190886094	25.79568341867565	24.336861821412644
24-25	24.687116537001742	25.220904711186158	25.718705753598048	24.37327299821405
26-27	24.38556923408607	25.46042860885044	25.89399166776977	24.260010489293716
28-29	24.428708615494337	25.68483061683413	25.71403918471794	24.17242158295359
30-31	24.91676847668617	25.417351275351113	25.538476229646545	24.12740401831617
32-33	24.62638976529649	25.366099499098155	25.563305170401556	24.444205565203795
34-35	24.76218038091624	25.47431648629078	25.71375202653266	24.049751106260324
36-37	24.760758236625506	25.448687363495324	25.679630312602164	24.110924087277002
38-39	24.712212680343463	25.59211734965957	25.651294184392725	24.04437578560424
40-41	24.73141380456476	25.39078155473371	25.676045982364275	24.20175865833725
42-43	24.56498311298521	25.32197516523312	25.519479938136904	24.593561783644773
44-45	24.426184251487946	25.35378813073162	25.677878896284522	24.542148721495913
46-47	24.594179740630278	25.23463903972364	25.55651204016769	24.614669179478394
48-49	24.364467315032357	25.513710628105603	25.583373180798308	24.538448876063732
50-51	24.452846601811398	25.463380455031132	25.693818983312543	24.389953959844927
52-53	24.644192755561566	25.31247163465109	25.66854111652167	24.37479449326567
54-55	24.234740937008528	25.204336445879388	25.863502512903676	24.697420104208405
56-57	24.29897051560382	25.30638284242095	25.64642978683109	24.748216855144143
58-59	24.442857712322358	25.28553835093464	25.791639071747724	24.47996486499528
60-61	24.682877593065864	25.30823331413774	25.775249884217992	24.2336392085784
62-63	24.536150016462916	25.270393244078313	25.85062359061639	24.34283314884238
64-65	24.331856729829116	25.014078321064048	25.918154110408164	24.73591083869867
66-67	24.39928050862854	25.22864522225086	25.637397551430052	24.734676717690547
68-69	24.24294044402823	25.284361922879455	25.7590804979657	24.713617135126615
70-71	24.299588825863193	25.05741671220663	25.82939601422301	24.813598447707164
72-73	24.28904681866168	25.372728357170338	25.586077591939382	24.752147232228598
74-75	24.133753019970545	25.40237838587714	25.770061023123226	24.69380757102909
76-77	24.16123694345774	25.010806306380008	25.814787110520232	25.01316963964202
78-79	24.095211571556323	24.96930010391595	25.88582785929369	25.049660465234037
80-81	24.27466027755267	25.25196865484334	25.602718031382928	24.870653036221064
82-83	24.35832459851179	25.15975525806414	25.745129354861678	24.73679078856239
84-85	24.331020486896534	25.20832893289621	25.60374580128358	24.85690477892367
86-87	24.005975041317296	25.35622133619149	25.74942292892026	24.888380693570955
88-89	24.018199015957087	25.06193812720704	25.866360822170662	25.053502034665204
90-91	24.230871358752662	24.91379978494365	25.910484962423897	24.944843893879792
92-93	24.4389999100402	24.808366737146837	25.853621273716055	24.89901207909691
94-95	24.353635212068323	24.75030352842967	25.887137656596813	25.0089236029052
96-97	24.177022131614333	24.69904670488429	25.980587088748937	25.143344074752445
98-99	23.997586857664196	24.66247416763868	25.94291703969779	25.397021934999337
100-101	24.272715163143065	24.643101805884992	25.998634924224024	25.08554810674792
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	72.0
2	82.0
3	123.0
4	128.0
5	151.5
6	264.5
7	502.5
8	738.5
9	822.5
10	754.5
11	665.0
12	719.0
13	816.5
14	901.5
15	981.0
16	1096.0
17	1252.5
18	1376.0
19	1480.0
20	1605.0
21	1749.0
22	1832.5
23	1960.0
24	2222.5
25	2600.0
26	3239.5
27	4186.0
28	5519.0
29	7819.0
30	11305.5
31	15554.0
32	21592.5
33	28783.0
34	36470.0
35	44776.5
36	56121.0
37	67507.0
38	75738.5
39	83794.5
40	89856.0
41	94916.0
42	100448.5
43	108794.0
44	118321.5
45	126650.5
46	133797.0
47	140063.5
48	147510.0
49	153323.5
50	156076.5
51	157144.0
52	157366.5
53	157470.5
54	157105.0
55	152869.5
56	144111.0
57	131890.0
58	119927.0
59	106786.0
60	90462.0
61	75908.0
62	62770.5
63	49264.5
64	36881.0
65	27772.0
66	21375.5
67	16682.0
68	13091.5
69	10161.5
70	7975.0
71	6182.5
72	4862.0
73	4073.0
74	3264.0
75	2550.5
76	2017.5
77	1378.0
78	866.5
79	614.0
80	437.0
81	305.0
82	219.0
83	170.0
84	136.5
85	110.5
86	95.0
87	83.5
88	73.5
89	60.0
90	52.5
91	49.5
92	37.0
93	28.5
94	23.5
95	18.5
96	15.0
97	15.5
98	15.5
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05044829747465371
2	0.05837708357470996
3	0.049303648777110375
4	0.05575276704863501
5	0.0479914905140729
6	0.017337240028644136
7	0.014210394806086738
8	0.018900662639922832
9	0.01934735481457389
10-11	0.02106432786088889
12-13	0.026187328738918193
14-15	0.04263118441826022
16-17	0.028337034829426407
18-19	0.02227877221072145
20-21	0.026340879173954494
22-23	0.04461338094327428
24-25	0.04016041832722156
26-27	0.030458822659018926
28-29	0.020771186121274134
30-31	0.012828440890760032
32-33	0.009631800015913409
34-35	0.009394494798130034
36-37	0.00134007652395317
38-39	0.006895810446175687
40-41	0.0033641504403407706
42-43	0.21053160556522613
44-45	0.1857122716111768
46-47	0.19186824814308667
48-49	0.18966270553074707
50-51	0.18830866987633607
52-53	0.1920915942304122
54-55	0.19089110901103748
56-57	0.20189090381181976
58-59	0.1982894481536956
60-61	0.20323098033577291
62-63	0.20056478641832443
64-65	0.20212820902960313
66-67	0.19150531075118268
68-69	0.19038858031455505
70-71	0.17507541420229852
72-73	0.18220852986625757
74-75	0.18199914290938987
76-77	0.1794167037746885
78-79	0.2055202777308596
80-81	0.21442620296296505
82-83	0.21188564121963713
84-85	0.20741871947312657
86-87	0.2238625751524686
88-89	0.22210372471478007
90-91	0.2216151551487555
92-93	0.22520265167642176
94-95	0.23720750387016895
96-97	0.23702603517421691
98-99	0.2267381560267848
100-101	0.23610473256399914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3581885.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.50988368592563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40213541260783	40.34759495599059
2	13.036212642391648	13.951324443987403
3	4.561292549849847	7.322227013998332
4	2.1464393406957183	4.594228778380911
5	1.1784989169100595	3.15306699839233
6	0.7463802332252578	2.3963231679214556
7	0.5075719703664758	1.9012081967582344
8	0.37429455590210475	1.602276652047745
9	0.29746041132623646	1.4325364810111059
>10	1.605694608602309	15.79174049971156
>50	0.10575680759094938	3.858740233891074
>100	0.03747660174881156	3.2626862796592895
>500	5.239658569242356E-4	0.1726540138122198
>1k	2.619829259005707E-4	0.21339228443773578
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.791826091569104E-5
2	0.0	0.0	0.0	0.0	2.791826091569104E-5
3	0.0	0.0	0.0	0.0	2.791826091569104E-5
4	0.0	0.0	0.0	0.0	2.791826091569104E-5
5	0.0	0.0	0.0	0.0	2.791826091569104E-5
6	0.0	0.0	0.0	0.0	2.791826091569104E-5
7	0.0	0.0	0.0	0.0	2.791826091569104E-5
8	0.0	0.0	0.0	0.0	5.583652183138208E-5
9	0.0	0.0	0.0	0.0	5.583652183138208E-5
10-11	0.0	0.0	0.0	2.791826091569104E-5	5.583652183138208E-5
12-13	0.0	0.0	0.0	5.583652183138208E-5	8.375478274707312E-5
14-15	0.0	0.0	0.0	5.583652183138208E-5	8.375478274707312E-5
16-17	0.0	0.0	0.0	5.583652183138208E-5	1.1167304366276416E-4
18-19	0.0	0.0	0.0	1.5355043503630073E-4	1.1167304366276416E-4
20-21	0.0	0.0	0.0	1.954278264098373E-4	1.5355043503630073E-4
22-23	0.0	0.0	0.0	2.791826091569104E-4	1.8146869595199176E-4
24-25	0.0	1.395913045784552E-5	0.0	6.281608706030484E-4	1.954278264098373E-4
26-27	0.0	2.791826091569104E-5	0.0	0.0010748530452541051	2.5126434824121935E-4
28-29	0.0	2.791826091569104E-5	0.0	0.0038806382672810546	2.5126434824121935E-4
30-31	0.0	2.791826091569104E-5	0.0	0.013847457414182755	2.791826091569104E-4
32-33	0.0	2.791826091569104E-5	0.0	0.03542827310201193	3.629373919039835E-4
34-35	0.0	2.791826091569104E-5	0.0	0.06450514184570415	3.908556528196746E-4
36-37	0.0	2.791826091569104E-5	0.0	0.110332967138811	4.187739137353656E-4
38-39	0.0	2.791826091569104E-5	0.0	0.1839394620430304	4.187739137353656E-4
40-41	0.0	2.791826091569104E-5	0.0	0.2672615117459103	4.187739137353656E-4
42-43	0.0	2.791826091569104E-5	0.0	0.3549806875430116	4.187739137353656E-4
44-45	0.0	2.791826091569104E-5	0.0	0.4592833103240333	4.327330441932111E-4
46-47	0.0	2.791826091569104E-5	0.0	0.5808115000900365	5.304469573981297E-4
48-49	0.0	4.187739137353656E-5	0.0	0.7093611324763358	5.862834792295118E-4
50-51	0.0	5.583652183138208E-5	0.0	0.8450857579179678	5.862834792295118E-4
52-53	0.0	5.583652183138208E-5	0.0	0.9893394120693433	6.560791315187394E-4
54-55	0.0	8.375478274707312E-5	0.0	1.155997470605561	7.817113056393492E-4
56-57	0.0	8.375478274707312E-5	0.0	1.344068835264114	8.235886970128857E-4
58-59	0.0	8.375478274707312E-5	0.0	1.5447452947261011	8.515069579285767E-4
60-61	0.0	8.375478274707312E-5	0.0	1.7617539368237674	8.933843493021133E-4
62-63	0.0	8.375478274707312E-5	0.0	1.9816939963175813	0.0010050573929648774
64-65	0.0	8.375478274707312E-5	0.0	2.226718613244144	0.001019016523422723
66-67	0.0	8.375478274707312E-5	0.0	2.509837697190167	0.001046934784338414
68-69	0.0	8.375478274707312E-5	0.0	2.8181669707430586	0.0010888121757119506
70-71	0.0	8.375478274707312E-5	0.0	3.1448385417175593	0.0011167304366276416
72-73	0.0	8.375478274707312E-5	0.0	3.4842827170609887	0.0011586078280011781
74-75	0.0	8.375478274707312E-5	0.0	3.843102165479908	0.0012004852193747148
76-77	0.0	8.375478274707312E-5	0.0	4.24071403744118	0.0012284034802904058
78-79	0.0	1.1167304366276416E-4	0.0	4.68563340252409	0.0012284034802904058
80-81	0.0	1.1167304366276416E-4	0.0	5.1526361119913116	0.0012423626107482513
82-83	0.0	1.1167304366276416E-4	0.0	5.629158948430785	0.0012842400021217878
84-85	0.0	1.1167304366276416E-4	0.0	6.133460454481369	0.0012842400021217878
86-87	0.0	1.1167304366276416E-4	0.0	6.671249914500326	0.0013121582630374789
88-89	0.0	1.1167304366276416E-4	0.0	7.262209702433216	0.0013400765239531699
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3165	0.0	54.129776	1
GTATCAA	6105	0.0	45.630493	1
TCAACGC	7575	0.0	36.465275	4
CAACGCA	7775	0.0	35.40171	5
ATCAACG	8135	0.0	33.8953	3
TATCAAC	8170	0.0	33.867695	2
AACGCAG	8370	0.0	32.875	6
GTACATG	13705	0.0	29.849081	1
TACATGG	14565	0.0	27.893461	2
ACGCAGA	9915	0.0	27.132889	7
GTATAGG	1270	0.0	26.904907	1
ACATGGG	15150	0.0	26.314045	3
CGCAGAG	10295	0.0	25.672304	8
CATGGGG	12180	0.0	24.821688	4
GCAGAGT	11540	0.0	21.545744	9
GAGTACA	10190	0.0	21.330154	1
GTATATG	980	0.0	20.823088	1
GTATACG	185	6.6097364E-6	20.52206	1
AGTACAT	10115	0.0	20.129387	2
ATGGGGG	7295	0.0	20.03651	5
>>END_MODULE