FastQCFastQC Report
Mon 15 Aug 2016
SRR2921418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581885
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.055.9258881
GTATCAA55300.044.61431
TCAACGC73400.033.3023874
CAACGCA76650.031.6372855
TATCAAC79500.030.9266512
ATCAACG79750.030.6507233
AACGCAG80250.030.2142376
GTACATG139300.028.1536481
TACATGG143600.026.7401812
ACATGGG150050.025.274063
CATGGGG124100.023.6746234
ACGCAGA102100.023.4681667
CGCAGAG106900.022.1920388
ATGGGGG71300.021.607115
GAGTACA105550.019.7263951
GTATAGG10750.019.4570061
GCAGAGT120150.018.557679
AGTACAT105650.018.5096422
TGGGGGG54650.018.182046
AGAGTAC103950.017.31074310-11
Produced by FastQC (version 0.11.5)