##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3581885
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.416539056949066	33.0	31.0	34.0	29.0	34.0
2	31.542309984826424	34.0	31.0	34.0	29.0	34.0
3	31.623362838282077	34.0	31.0	34.0	28.0	34.0
4	35.110348880547534	37.0	35.0	37.0	33.0	37.0
5	34.846080485554396	37.0	35.0	37.0	32.0	37.0
6	34.80011362732193	37.0	35.0	37.0	32.0	37.0
7	34.66049803385648	37.0	35.0	37.0	32.0	37.0
8	34.62766197128048	37.0	35.0	37.0	32.0	37.0
9	36.248895762985136	39.0	37.0	39.0	32.0	39.0
10-11	36.0963903084549	39.0	37.0	39.0	32.0	39.0
12-13	36.023207752342685	39.0	37.0	39.0	31.0	39.0
14-15	37.26086892795274	40.0	38.0	41.0	31.0	41.0
16-17	37.23210879187914	40.0	38.0	41.0	31.0	41.0
18-19	37.189156407869035	40.0	38.0	41.0	31.0	41.0
20-21	37.14615614404147	40.0	38.0	41.0	31.0	41.0
22-23	37.03506128756227	40.0	38.0	41.0	31.0	41.0
24-25	36.962627080433904	40.0	37.0	41.0	30.5	41.0
26-27	36.8250953059632	40.0	37.0	41.0	30.0	41.0
28-29	36.66806625561681	40.0	37.0	41.0	30.0	41.0
30-31	36.53038079111976	40.0	37.0	41.0	30.0	41.0
32-33	36.48685552439567	40.0	36.5	41.0	30.0	41.0
34-35	36.62887180911727	40.0	37.0	41.0	30.0	41.0
36-37	36.612464247177115	40.0	37.0	41.0	30.0	41.0
38-39	36.52603377830388	40.0	37.0	41.0	30.0	41.0
40-41	36.402431959708366	40.0	36.5	41.0	30.0	41.0
42-43	36.30365980482344	40.0	36.0	41.0	29.5	41.0
44-45	36.16689969108444	40.0	36.0	41.0	29.0	41.0
46-47	36.00570174642681	40.0	36.0	41.0	28.0	41.0
48-49	35.84352373121973	40.0	35.0	41.0	28.0	41.0
50-51	35.680408639585025	39.5	35.0	41.0	27.5	41.0
52-53	35.50072419968815	39.0	35.0	41.0	27.0	41.0
54-55	35.282693330467055	39.0	35.0	41.0	26.5	41.0
56-57	35.004878576503714	39.0	35.0	41.0	26.0	41.0
58-59	34.77045103346423	38.0	35.0	40.0	26.0	41.0
60-61	34.48210648862261	38.0	34.0	40.0	26.0	41.0
62-63	34.16994180438512	37.0	34.0	40.0	25.5	41.0
64-65	33.84453856000402	37.0	34.0	39.5	25.0	41.0
66-67	33.50035023458319	36.0	34.0	39.0	23.5	41.0
68-69	33.13375122316881	35.5	33.5	39.0	23.0	40.5
70-71	32.757707045312735	35.0	33.0	38.5	22.5	40.0
72-73	32.36692858648449	35.0	33.0	37.0	21.5	39.5
74-75	31.97156776390085	35.0	33.0	37.0	20.5	39.0
76-77	29.249128461689864	33.0	28.5	34.5	14.0	36.5
78-79	30.91941072926685	34.5	31.5	35.5	18.0	37.0
80-81	31.126463161156764	35.0	32.0	35.0	19.0	37.0
82-83	30.983287431059345	35.0	32.0	35.0	18.0	37.0
84-85	30.768816279696303	35.0	32.0	35.0	17.0	36.0
86-87	30.541782190103817	35.0	32.0	35.0	12.0	36.0
88-89	30.282688444771395	35.0	32.0	35.0	7.0	35.5
90-91	30.112133834559177	35.0	32.0	35.0	3.5	35.0
92-93	29.979093689495894	34.0	31.0	35.0	2.0	35.0
94-95	29.883073158406816	34.0	31.0	35.0	2.0	35.0
96-97	29.702226481308024	34.0	31.0	35.0	2.0	35.0
98-99	29.443680631846078	34.0	31.0	35.0	2.0	35.0
100-101	28.34325222613233	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67382.0
3	23457.0
4	12895.0
5	9232.0
6	7948.0
7	8200.0
8	8136.0
9	8609.0
10	9630.0
11	10217.0
12	10917.0
13	11569.0
14	12124.0
15	13264.0
16	14491.0
17	15789.0
18	14879.0
19	12972.0
20	12797.0
21	12419.0
22	13257.0
23	15207.0
24	17524.0
25	21517.0
26	25809.0
27	31295.0
28	37674.0
29	45612.0
30	55444.0
31	70402.0
32	89156.0
33	118567.0
34	173688.0
35	261249.0
36	458208.0
37	882423.0
38	866183.0
39	101739.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81188838561762	18.985796566571604	13.089582284851534	26.112732762959233
2	15.44366058967506	20.386831351547833	37.43909258596973	26.730415472807366
3	18.37881408128405	24.341018220033046	28.477868654272793	28.802299044410105
4	13.41113707919627	15.94481687288354	34.516703720279516	36.12734232764067
5	14.611980661866388	37.3324724706387	33.50469602089305	14.550850846601865
6	34.58221624019603	34.3186925382785	16.60176987197366	14.49732134955181
7	30.054817505307955	31.29910647605939	20.201206906419387	18.44486911221326
8	27.707694287766703	34.568946287088835	19.149967377093162	18.573392048051293
9	27.607028143002914	15.179186378122134	18.69979075263444	38.51399472624051
10-11	26.55620908877249	24.946546249127035	26.783088856887588	21.714155805212883
12-13	26.73097647045686	22.112493997565526	27.058318536634392	24.098210995343226
14-15	24.07074622986117	23.640884304141725	24.125733601192607	28.162635864804496
16-17	24.37280178409004	26.86589061281192	24.76647751098445	23.994830092113588
18-19	24.48399574021926	26.305444258306647	25.999152119930503	23.21140788154359
20-21	24.94497272366871	25.35090707062811	26.02746896863641	23.676651237066775
22-23	24.851219744734358	24.956360164846554	25.76847638381725	24.423943706601833
24-25	24.564192197526094	25.33872741706708	25.705844687297024	24.391235698109803
26-27	24.286748880382312	25.52927952765688	25.839290993949138	24.344680598011674
28-29	24.33707315867848	25.78925745185875	25.68801985999003	24.185649529472744
30-31	24.859387519159835	25.477179153151763	25.48605839834891	24.1773749293395
32-33	24.574490675324025	25.493995408758817	25.485618760007476	24.445895155909678
34-35	24.650713845158993	25.539373098397633	25.760661886758108	24.049251169685267
36-37	24.60904755809235	25.561736543973012	25.717786687173692	24.11142921076094
38-39	24.616720183713557	25.695846078617084	25.587941864615527	24.099491873053832
40-41	24.69351231674256	25.44797038286182	25.626488465006936	24.232028835388682
42-43	24.428768887066603	25.412467582434534	25.4989516511477	24.659811879351164
44-45	24.32223216969328	25.41039367554899	25.633953509215107	24.633420645542632
46-47	24.484994112902168	25.35880304705127	25.520199153385914	24.636003686660647
48-49	24.2783408509288	25.56169065554205	25.578136340874103	24.58183215265505
50-51	24.352038448845715	25.502415895941976	25.67215401427369	24.473391640938615
52-53	24.55843800515379	25.404848918011186	25.563343318917987	24.473369757917045
54-55	24.109865664278793	25.246178856885848	25.83146348797143	24.81249199086393
56-57	24.1899867938699	25.359957785055578	25.583247479527703	24.86680794154682
58-59	24.316890571402762	25.389458265991593	25.702367874427395	24.591283288178246
60-61	24.56752322315518	25.466127858474763	25.661347408573548	24.305001509796504
62-63	24.39124607329843	25.353075043630017	25.767650959860383	24.48802792321117
64-65	24.142215986312152	25.14632287116368	25.796071125308234	24.915390017215934
66-67	24.197945512786053	25.428688448016345	25.486960780192934	24.88640525900467
68-69	24.037590740965697	25.43096984446799	25.582969252443842	24.94847016212246
70-71	24.089908967156788	25.14933810291733	25.69471404001602	25.06603888990986
72-73	24.075127733371275	25.462242402215363	25.429863128804026	25.03276673560934
74-75	23.852064289035372	25.522275109152016	25.539365235833973	25.08629536597864
76-77	23.81947448747446	25.098052987789828	25.708197778249136	25.374274746486574
78-79	23.729901192459522	25.10174158120281	25.71135995476507	25.4569972715726
80-81	23.912517917125985	25.414029987783604	25.37375093382741	25.299701161263005
82-83	23.933469173596205	25.308453920188583	25.542693205873952	25.21538370034126
84-85	23.86598732774491	25.2757533646714	25.410781470625032	25.447477836958658
86-87	23.48150186706602	25.461877068406004	25.551186107296576	25.505434957231405
88-89	23.487265035193552	25.137313982185955	25.68850120040837	25.686919782212126
90-91	23.616720386897676	24.97674301380297	25.77172308924466	25.634813510054695
92-93	23.71533028578196	24.877742279496175	25.681229962386965	25.725697472334897
94-95	23.58862186574465	24.844055021732625	25.69801815444681	25.86930495807592
96-97	23.314689131847523	24.833308553507322	25.778418097788876	26.073584216856283
98-99	23.079805123404583	24.790901031350643	25.765242130721877	26.3640517145229
100-101	23.083424906584117	24.862137587692555	25.80673800446252	26.247699501260808
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	75.0
1	59.0
2	61.5
3	101.0
4	102.0
5	105.0
6	191.0
7	409.0
8	597.0
9	649.0
10	573.0
11	451.0
12	437.5
13	493.0
14	542.5
15	600.5
16	673.5
17	736.0
18	847.5
19	955.0
20	1014.0
21	1127.0
22	1296.5
23	1495.0
24	1774.0
25	2216.5
26	2985.0
27	4042.5
28	5518.0
29	7876.0
30	11549.0
31	15903.0
32	21683.0
33	28817.0
34	36504.5
35	44806.5
36	56164.0
37	67469.5
38	75254.0
39	83367.0
40	89583.5
41	94043.0
42	100006.5
43	108827.5
44	118573.5
45	127460.0
46	134681.5
47	141478.5
48	149656.0
49	155270.5
50	157515.5
51	158764.5
52	159155.5
53	159248.0
54	158485.0
55	153602.5
56	144812.0
57	133449.0
58	121345.5
59	107016.5
60	90557.0
61	75824.5
62	62339.5
63	48537.0
64	35844.0
65	26587.5
66	20348.0
67	15803.5
68	12322.0
69	9583.5
70	7451.0
71	5831.0
72	4560.0
73	3787.0
74	3030.5
75	2321.5
76	1737.0
77	1116.0
78	662.0
79	402.0
80	262.0
81	174.5
82	108.0
83	60.5
84	47.0
85	32.5
86	20.0
87	18.0
88	16.0
89	9.5
90	9.0
91	8.5
92	5.5
93	4.0
94	2.5
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14754800893942716
2	3.908556528196745E-4
3	0.006225772184199101
4	0.0038527200063653635
5	0.02738781395829291
6	0.0012284034802904058
7	0.0
8	0.0012842400021217878
9	0.0
10-11	0.09933317233802871
12-13	1.395913045784552E-4
14-15	0.0042854530505585745
16-17	8.375478274707313E-5
18-19	2.931417396147559E-4
20-21	6.97956522892276E-4
22-23	2.3730521778337382E-4
24-25	0.001661136524483617
26-27	0.0022892973950866652
28-29	0.005904712183668655
30-31	0.013945171327387675
32-33	0.013973089588303365
34-35	0.01021808349514292
36-37	6.560791315187394E-4
38-39	1.2563217412060967E-4
40-41	0.004885695660245932
42-43	0.008389437405165158
44-45	0.00819400957875532
46-47	0.009045516536683897
48-49	0.01112542697490288
50-51	0.005332387834896989
52-53	0.001535504350363007
54-55	0.0010608939147962594
56-57	0.006002426096873573
58-59	0.009478249580877108
60-61	0.007719399143188573
62-63	0.01772809568146381
64-65	0.03323668962013018
66-67	0.03583308788528945
68-69	0.02652234786990649
70-71	0.03961601223936559
72-73	0.024861211345422872
74-75	0.024288886996651205
76-77	0.23191699342664548
78-79	0.0656218722823318
80-81	0.05194192443364318
82-83	0.020045311337466166
84-85	0.032078081792129
86-87	0.07649603490899344
88-89	0.2552427004217053
90-91	0.3047278178947677
92-93	0.048661528776049484
94-95	0.00482985913841455
96-97	0.00956200436362418
98-99	7.956704360971945E-4
100-101	0.001479667828531625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3581885.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.65929117082734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55101112954642	39.258034021503754
2	13.50971764437723	14.228243101418487
3	4.720335968081002	7.457086385019197
4	2.1848261750378977	4.602055908358625
5	1.2336440449160124	3.2481410481194755
6	0.7671947352712891	2.423995856962597
7	0.5451881290484247	2.0096454301307713
8	0.38107603878966584	1.6053755267880405
9	0.2947683602320484	1.3970063618466082
>10	1.6609475026290355	16.04902891603352
>50	0.11017181750842932	3.9585533377565154
>100	0.040319876885239525	3.3868844505642253
>500	5.323851222254353E-4	0.17482640384661355
>1k	2.661925550992837E-4	0.20112325165145042
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.791826091569104E-5	0.0
7	0.0	0.0	0.0	2.791826091569104E-5	0.0
8	0.0	0.0	0.0	2.791826091569104E-5	0.0
9	0.0	0.0	0.0	8.375478274707312E-5	2.791826091569104E-5
10-11	0.0	1.395913045784552E-5	0.0	9.771391320491864E-5	4.187739137353656E-5
12-13	0.0	2.791826091569104E-5	0.0	1.6750956549414623E-4	1.8146869595199176E-4
14-15	0.0	2.791826091569104E-5	0.0	1.6750956549414623E-4	2.9314173961475596E-4
16-17	0.0	2.791826091569104E-5	0.0	1.6750956549414623E-4	3.908556528196746E-4
18-19	0.0	5.583652183138208E-5	0.0	1.8146869595199176E-4	4.187739137353656E-4
20-21	0.0	8.375478274707312E-5	0.0	1.954278264098373E-4	4.746104355667477E-4
22-23	0.0	8.375478274707312E-5	0.0	2.791826091569104E-4	4.885695660245932E-4
24-25	0.0	8.375478274707312E-5	0.0	6.281608706030484E-4	5.304469573981298E-4
26-27	0.0	8.375478274707312E-5	0.0	0.0011027713061697961	5.304469573981298E-4
28-29	0.0	8.375478274707312E-5	0.0	0.0039643930500281276	5.304469573981298E-4
30-31	0.0	8.375478274707312E-5	0.0	0.014098721762423974	5.304469573981298E-4
32-33	0.0	8.375478274707312E-5	0.0	0.03620998440765128	5.304469573981298E-4
34-35	0.0	8.375478274707312E-5	0.0	0.0655939540214161	5.304469573981298E-4
36-37	0.0	8.375478274707312E-5	0.0	0.11210577670695737	5.304469573981298E-4
38-39	0.0	8.375478274707312E-5	0.0	0.1864521055254426	5.444060878559753E-4
40-41	0.0	8.375478274707312E-5	0.0	0.27002541957656373	5.583652183138208E-4
42-43	0.0	8.375478274707312E-5	0.0	0.3575491675472552	5.862834792295118E-4
44-45	0.0	8.375478274707312E-5	0.0	0.46199138163285536	6.002426096873574E-4
46-47	0.0	9.771391320491864E-5	0.0	0.583561448790232	6.281608706030484E-4
48-49	0.0	1.1167304366276416E-4	0.0	0.7130463429172069	6.421200010608939E-4
50-51	0.0	1.1167304366276416E-4	0.0	0.8484499083583086	6.700382619765849E-4
52-53	0.0	1.1167304366276416E-4	0.0	0.9929967042492989	6.839973924344304E-4
54-55	0.0	1.1167304366276416E-4	0.0	1.160143332351541	7.119156533501215E-4
56-57	0.0	1.1167304366276416E-4	0.0	1.3487730622284078	7.398339142658125E-4
58-59	0.0	1.1167304366276416E-4	0.0	1.5495053582122262	7.53793044723658E-4
60-61	0.0	1.1167304366276416E-4	0.0	1.7676446898769782	7.53793044723658E-4
62-63	0.0	1.1167304366276416E-4	0.0	1.9890923354602394	8.096295665550402E-4
64-65	0.0	1.1167304366276416E-4	0.0	2.234731154126947	8.235886970128857E-4
66-67	0.0	1.1167304366276416E-4	0.0	2.518464439813115	8.375478274707312E-4
68-69	0.0	1.1167304366276416E-4	0.0	2.826765795105091	8.375478274707312E-4
70-71	2.791826091569104E-5	1.1167304366276416E-4	0.0	3.1553637260827747	8.375478274707312E-4
72-73	2.791826091569104E-5	1.1167304366276416E-4	0.0	3.4963852831679407	8.794252188442678E-4
74-75	2.791826091569104E-5	1.1167304366276416E-4	0.0	3.8581082307220917	8.933843493021133E-4
76-77	2.791826091569104E-5	1.1167304366276416E-4	0.0	4.259796168777054	8.933843493021133E-4
78-79	2.791826091569104E-5	1.1167304366276416E-4	0.0	4.708903272997318	9.213026102178043E-4
80-81	2.791826091569104E-5	1.1167304366276416E-4	0.0	5.180666045950666	9.771391320491864E-4
82-83	2.791826091569104E-5	1.1167304366276416E-4	0.0	5.662479392833662	0.0010888121757119506
84-85	2.791826091569104E-5	1.1167304366276416E-4	0.0	6.173718586721796	0.0010888121757119506
86-87	2.791826091569104E-5	1.1167304366276416E-4	0.0	6.71716149457618	0.0011865260889168693
88-89	2.791826091569104E-5	1.1167304366276416E-4	0.0	7.315100847737993	0.0012284034802904058
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3060	0.0	55.925888	1
GTATCAA	5530	0.0	44.6143	1
TCAACGC	7340	0.0	33.302387	4
CAACGCA	7665	0.0	31.637285	5
TATCAAC	7950	0.0	30.926651	2
ATCAACG	7975	0.0	30.650723	3
AACGCAG	8025	0.0	30.214237	6
GTACATG	13930	0.0	28.153648	1
TACATGG	14360	0.0	26.740181	2
ACATGGG	15005	0.0	25.27406	3
CATGGGG	12410	0.0	23.674623	4
ACGCAGA	10210	0.0	23.468166	7
CGCAGAG	10690	0.0	22.192038	8
ATGGGGG	7130	0.0	21.60711	5
GAGTACA	10555	0.0	19.726395	1
GTATAGG	1075	0.0	19.457006	1
GCAGAGT	12015	0.0	18.55767	9
AGTACAT	10565	0.0	18.509642	2
TGGGGGG	5465	0.0	18.18204	6
AGAGTAC	10395	0.0	17.310743	10-11
>>END_MODULE