FastQCFastQC Report
Mon 15 Aug 2016
SRR2921417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064764
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.052.909521
GTATCAA45350.042.6898461
TCAACGC54900.034.4898954
CAACGCA57050.033.0221185
ATCAACG57750.032.7856563
TATCAAC59950.031.7418562
GTACATG100250.030.5766471
AACGCAG61600.030.57456
TACATGG101200.029.354252
ACATGGG107950.027.2980673
CATGGGG87850.024.9569534
ACGCAGA73950.024.8951037
CGCAGAG76250.023.4604388
ATGGGGG55750.021.8755175
GAGTACA74050.019.8004531
GCAGAGT87750.019.3587639
AGTACAT75400.018.2517192
AGAGTAC75150.018.21682710-11
TATCTCG3702.7866918E-916.672925
GTGGTAT14200.016.3737981
Produced by FastQC (version 0.11.5)