##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921417_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3064764
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.36964118607501	31.0	31.0	34.0	26.0	34.0
2	30.411406881573917	31.0	31.0	34.0	26.0	34.0
3	30.464042255782175	33.0	31.0	34.0	26.0	34.0
4	33.92231832532619	37.0	35.0	37.0	30.0	37.0
5	33.916071514805054	37.0	35.0	37.0	30.0	37.0
6	33.96175039905194	37.0	35.0	37.0	30.0	37.0
7	33.87670372009068	37.0	35.0	37.0	29.0	37.0
8	33.844244124506815	37.0	35.0	37.0	29.0	37.0
9	35.40012412048693	39.0	37.0	39.0	30.0	39.0
10-11	35.28989654668353	39.0	35.0	39.0	29.0	39.0
12-13	35.17167276175262	39.0	35.0	39.0	28.0	39.0
14-15	36.29690393779097	40.0	37.0	41.0	28.0	41.0
16-17	36.24233317801958	40.0	36.5	41.0	27.0	41.0
18-19	36.18974886810208	40.0	36.5	41.0	27.0	41.0
20-21	36.124440739972144	40.0	36.5	41.0	27.0	41.0
22-23	36.00754299515395	40.0	36.0	41.0	27.0	41.0
24-25	35.908096186199	40.0	36.0	41.0	27.0	41.0
26-27	35.75381791224382	40.0	36.0	41.0	27.0	41.0
28-29	35.61251437304797	40.0	36.0	41.0	26.0	41.0
30-31	35.46509927028639	40.0	35.5	41.0	25.0	41.0
32-33	35.27748906604228	39.0	35.0	41.0	25.0	41.0
34-35	35.11158053279143	39.0	35.0	41.0	24.0	41.0
36-37	34.94048367182596	39.0	35.0	41.0	24.0	41.0
38-39	35.17129752894513	39.0	35.0	41.0	24.5	41.0
40-41	35.28841812942204	40.0	35.0	41.0	24.5	41.0
42-43	35.12294160333389	40.0	35.0	41.0	24.0	41.0
44-45	35.00064605300767	40.0	35.0	41.0	23.0	41.0
46-47	34.8414848582142	39.5	35.0	41.0	22.5	41.0
48-49	34.68800844045414	39.0	35.0	41.0	21.5	41.0
50-51	32.96605464564318	37.0	32.5	39.5	17.0	40.0
52-53	33.119219457028336	37.5	32.5	39.5	16.5	40.0
54-55	33.97990970919784	38.5	34.0	40.5	17.5	41.0
56-57	33.99210493858581	38.5	34.0	41.0	17.0	41.0
58-59	33.81248050420848	38.0	34.0	41.0	15.5	41.0
60-61	33.5695802352155	38.0	34.0	40.0	13.5	41.0
62-63	33.277442406658395	37.0	33.0	40.0	11.5	41.0
64-65	32.98915756645536	37.0	33.0	40.0	8.0	41.0
66-67	32.64806131891395	36.0	33.0	39.0	7.0	41.0
68-69	32.28544139124578	36.0	33.0	39.0	3.5	41.0
70-71	31.90647925908814	35.0	32.0	39.0	2.0	40.5
72-73	31.547274765691583	35.0	32.0	37.5	2.0	40.0
74-75	31.149949066225002	35.0	32.0	37.0	2.0	39.0
76-77	30.776651318013393	35.0	31.5	36.5	2.0	39.0
78-79	30.429534378503533	35.0	31.0	36.0	2.0	38.5
80-81	30.077313620233078	35.0	31.0	36.0	2.0	37.0
82-83	29.777158208592894	35.0	31.0	35.0	2.0	37.0
84-85	29.540752077484598	35.0	31.0	35.0	2.0	36.5
86-87	29.31074366574392	35.0	30.5	35.0	2.0	36.0
88-89	29.107089648664626	35.0	30.0	35.0	2.0	36.0
90-91	28.850770728186575	34.0	30.0	35.0	2.0	35.5
92-93	28.647377253191436	34.0	30.0	35.0	2.0	35.0
94-95	28.433591297731244	34.0	30.0	35.0	2.0	35.0
96-97	28.167234736508263	34.0	29.0	35.0	2.0	35.0
98-99	27.930681122592148	34.0	29.0	35.0	2.0	35.0
100-101	26.871879531344014	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107905.0
3	18424.0
4	12857.0
5	9879.0
6	8672.0
7	9020.0
8	9621.0
9	10311.0
10	11202.0
11	11827.0
12	12000.0
13	11998.0
14	12952.0
15	13702.0
16	14082.0
17	14906.0
18	13909.0
19	12959.0
20	13046.0
21	13862.0
22	15239.0
23	16931.0
24	20256.0
25	23661.0
26	28232.0
27	33222.0
28	39100.0
29	46479.0
30	55320.0
31	67323.0
32	83992.0
33	108376.0
34	153797.0
35	221407.0
36	363869.0
37	645161.0
38	677526.0
39	121724.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32151384511724	17.880509759908282	12.594744268008512	25.20323212696597
2	16.18008055482733	19.54468272517823	39.03043643060561	25.244800289388824
3	19.55747985896131	23.390536853881454	29.121563765292873	27.93041952186436
4	13.604445220718464	15.192135981443425	36.17915388671151	35.0242649111266
5	14.436205340310545	37.13812369320064	33.967071512630966	14.458599453857843
6	33.965114480349484	34.523173141126186	17.03877104386994	14.472941334654399
7	29.50222154054006	31.646844400787778	20.524358625010812	18.326575433661358
8	27.067534212911305	34.47409550732494	19.798206402423403	18.660163877340352
9	27.381330802524456	15.456906516941645	19.039582834275315	38.12217984625858
10-11	26.117291360292317	24.95483120119632	27.4390335443604	21.488843894150968
12-13	26.45301654515018	22.376391151332655	27.294194135027723	23.87639816848944
14-15	23.676635942045355	23.9589860336349	24.60318950001722	27.761188524302526
16-17	23.905659287343198	27.04187992092253	25.47199894511153	23.58046184662274
18-19	23.92650130548303	26.564050261096604	26.649543080939946	22.859905352480418
20-21	24.4250410221638	25.67136412259895	26.67454879699598	23.229046058241273
22-23	24.29599499884928	25.443125784490228	26.308354239470898	23.952524977189597
24-25	24.003390961396967	25.586040912203874	26.427481150263198	23.98308697613596
26-27	23.792019035247193	25.88129408347178	26.407619321167573	23.919067560113454
28-29	23.854771530324605	26.203295380837183	26.212188584879105	23.729744503959108
30-31	24.35200189012876	25.79392668865399	26.071066165438804	23.783005255778445
32-33	23.986415102959523	25.826609005093115	26.050712489180295	24.13626340276707
34-35	24.2999100489795	25.928132838387892	26.13877453522995	23.633182577402664
36-37	24.285202469137413	25.81346905453494	26.179343325347816	23.72198515097983
38-39	24.140438580962737	25.963647102299937	26.14569711465904	23.75021720207829
40-41	24.315843966333983	25.755836709348767	26.116157119603372	23.812162204713875
42-43	24.090500356416477	25.72097494621054	25.972117767851888	24.216406929521096
44-45	23.94032176179506	25.813942008603547	26.18619136958147	24.059544860019923
46-47	24.06050569400643	25.711143945482956	26.00477346163258	24.223576898878036
48-49	23.896258652127468	25.89694027917286	26.042631930385774	24.164169138313895
50-51	24.049584507366866	25.925471877299643	26.06411653343245	23.960827081901037
52-53	24.202092053834154	25.732644667479303	26.08237737818652	23.982885900500023
54-55	23.639754434194828	25.60858973585673	26.427730883232993	24.32392494671545
56-57	23.824447157115127	25.726351906408613	26.174580139555953	24.274620796920306
58-59	23.9076026435676	25.665174913702177	26.306874988965518	24.120347453764705
60-61	24.05842657981717	25.696336502781946	26.368665911823257	23.876571005577624
62-63	23.962944247826528	25.628321350166495	26.403727415799732	24.00500698620725
64-65	23.731385446562918	25.461141986873276	26.386107018683052	24.421365547880754
66-67	23.7361048359519	25.626831193168037	26.205326405993468	24.4317375648866
68-69	23.79008905527783	25.61255028678444	26.305413130933538	24.291947527004194
70-71	23.750943639221987	25.395274310344572	26.336780691135342	24.517001359298103
72-73	23.742922708788264	25.67989395504531	26.118653320606196	24.458530015560235
74-75	23.677561649173413	25.727341910192724	26.21721077289803	24.377885667735832
76-77	23.67048896899826	25.352900200088197	26.309734302098853	24.666876528814694
78-79	23.67630984288	25.40061464253916	26.36462708972087	24.558448424859964
80-81	23.892780314684735	25.537130938352913	26.07792839714768	24.49216034981466
82-83	23.870075156106534	25.560891357178544	26.1905984236314	24.37843506308352
84-85	23.801492674691836	25.60654316702664	26.134542792966958	24.457421365314563
86-87	23.6516386627242	25.73028080358727	26.112302140074927	24.505778393613607
88-89	23.62218165996016	25.42140304112317	26.27744554549021	24.678969753426465
90-91	23.798809183940758	25.262214007442356	26.315730093466012	24.623246715150874
92-93	23.927196461695843	25.11497068419249	26.387677026401235	24.570155827710437
94-95	23.83489171719319	25.117342829458156	26.413482098687197	24.634283354661456
96-97	23.786587897711783	24.912780728470008	26.46340551985597	24.837225853962238
98-99	23.57141478886206	24.87501197798471	26.452982689890714	25.10059054326252
100-101	23.876166353545766	24.86477036637762	26.490535070412953	24.76852820966366
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	55.0
2	93.0
3	153.0
4	146.5
5	162.0
6	261.0
7	436.5
8	664.5
9	768.5
10	675.5
11	618.5
12	656.5
13	687.5
14	772.0
15	859.5
16	967.5
17	1132.0
18	1243.0
19	1363.0
20	1509.0
21	1639.5
22	1809.5
23	2038.5
24	2373.0
25	2827.5
26	3520.5
27	4642.5
28	6487.5
29	9253.5
30	12934.5
31	18093.0
32	25458.0
33	31663.0
34	37001.0
35	44127.5
36	52967.5
37	61915.0
38	69903.0
39	77304.0
40	83214.5
41	88917.0
42	94337.5
43	100310.5
44	107427.0
45	114994.5
46	121093.0
47	125364.5
48	130243.5
49	133979.5
50	134441.5
51	133418.5
52	131601.0
53	129926.5
54	127564.5
55	121750.0
56	112651.5
57	101029.5
58	90532.0
59	79543.5
60	66902.5
61	55969.5
62	46260.5
63	36291.0
64	27062.0
65	20395.5
66	15637.5
67	12025.5
68	9278.5
69	7352.0
70	5948.0
71	4606.0
72	3495.0
73	2816.5
74	2316.5
75	1883.0
76	1416.5
77	943.5
78	624.5
79	451.0
80	322.0
81	239.5
82	183.5
83	129.5
84	108.5
85	93.5
86	69.5
87	65.0
88	54.0
89	41.5
90	36.0
91	31.5
92	29.5
93	28.0
94	24.0
95	23.0
96	20.5
97	14.0
98	10.5
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049530730588064856
2	0.05732904719580366
3	0.04864974921396884
4	0.055436568688486294
5	0.047605623140966155
6	0.018598495675360323
7	0.014650393961818919
8	0.020980408279397695
9	0.021469842376117702
10-11	0.022905515726496396
12-13	0.027571454115227143
14-15	0.04465270409075544
16-17	0.03044280081598453
18-19	0.024928509992939097
20-21	0.02843612101943249
22-23	0.04793191253877949
24-25	0.04326597415004875
26-27	0.03178058734701922
28-29	0.020442030773005687
30-31	0.013785727057613571
32-33	0.010816493537512187
34-35	0.010392317320354847
36-37	0.0016314469890666948
38-39	0.007227310161565459
40-41	0.004258076641464074
42-43	0.21352378192904903
44-45	0.18901944815326727
46-47	0.19503948754292338
48-49	0.19272283281844865
50-51	0.19136873181752329
52-53	0.19575732421811273
54-55	0.19415850616882738
56-57	0.20482816947732355
58-59	0.20132055845083013
60-61	0.20533391804393422
62-63	0.2035230118860702
64-65	0.20458345242896353
66-67	0.19466425473543802
68-69	0.1936038141925447
70-71	0.17830084143509908
72-73	0.18546289371710187
74-75	0.18533237795797652
76-77	0.18314623899262716
78-79	0.20885784354031828
80-81	0.21820603478767045
82-83	0.21522048679767838
84-85	0.20975513938430498
86-87	0.22678744595016126
88-89	0.2255801751782519
90-91	0.22386715583973185
92-93	0.22931618878321464
94-95	0.2406873742970095
96-97	0.24091577687547885
98-99	0.230719233193812
100-101	0.2405405440679935
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3064764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.859980283222455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47462322651504	48.36824002582239
2	11.591500828528151	14.109170237543603
3	3.618035374463254	6.605806846615052
4	1.6171571218391596	3.936806022000161
5	0.9217515495338299	2.804889056532932
6	0.5837654827168137	2.131677346090268
7	0.39246719399144225	1.6719881981691573
8	0.28367671920086596	1.3811647629899149
9	0.22528599704769833	1.233981120456813
>10	1.216185012654925	13.084978957295698
>50	0.05880964841119647	2.413499515008445
>100	0.015612207720838437	1.6343362696368435
>500	8.606760966901986E-4	0.4079476748729435
>1k	2.689612800177389E-4	0.21551396696579397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.2628939781333896E-5
7	0.0	3.2628939781333896E-5	0.0	0.0	3.2628939781333896E-5
8	0.0	3.2628939781333896E-5	0.0	0.0	3.2628939781333896E-5
9	0.0	3.2628939781333896E-5	0.0	0.0	9.78868193440017E-5
10-11	0.0	3.2628939781333896E-5	0.0	0.0	1.1420128923466863E-4
12-13	0.0	3.2628939781333896E-5	0.0	0.0	1.957736386880034E-4
14-15	0.0	3.2628939781333896E-5	0.0	0.0	2.773459881413381E-4
16-17	0.0	3.2628939781333896E-5	0.0	1.6314469890666948E-5	2.9366045803200507E-4
18-19	0.0	6.525787956266779E-5	0.0	3.2628939781333896E-5	3.2628939781333897E-4
20-21	0.0	6.525787956266779E-5	0.0	9.78868193440017E-5	3.589183375946729E-4
22-23	0.0	6.525787956266779E-5	0.0	2.1208810857867034E-4	3.7523280748533985E-4
24-25	0.0	6.525787956266779E-5	0.0	4.568051569386746E-4	3.915472773760068E-4
26-27	0.0	6.525787956266779E-5	0.0	0.0010604405428933515	4.731196268293415E-4
28-29	0.0	6.525787956266779E-5	0.0	0.004209133231792073	4.894340967200085E-4
30-31	0.0	6.525787956266779E-5	0.0	0.01709756444541896	4.894340967200085E-4
32-33	0.0	6.525787956266779E-5	0.0	0.04528896841649145	4.894340967200085E-4
34-35	0.0	6.525787956266779E-5	0.0	0.0830243372736041	5.546919762826763E-4
36-37	0.0	6.525787956266779E-5	0.0	0.1406796738672211	5.710064461733433E-4
38-39	0.0	6.525787956266779E-5	0.0	0.23284011427959866	6.199498558453441E-4
40-41	0.0	6.525787956266779E-5	0.0	0.33726903604975783	6.199498558453441E-4
42-43	0.0	6.525787956266779E-5	0.0	0.44752222357088506	6.199498558453441E-4
44-45	0.0	6.525787956266779E-5	0.0	0.5848411166406288	6.36264325736011E-4
46-47	0.0	6.525787956266779E-5	0.0	0.7374140390581461	6.688932655173449E-4
48-49	0.0	6.525787956266779E-5	0.0	0.8929072515860927	7.830945547520136E-4
50-51	0.0	8.157234945333474E-5	0.0	1.0588906682537382	7.830945547520136E-4
52-53	0.0	9.78868193440017E-5	0.0	1.235152200952504	8.157234945333474E-4
54-55	0.0	1.6314469890666949E-4	0.0	1.441677075298457	8.809813740960152E-4
56-57	0.0	1.957736386880034E-4	0.0	1.6746313908672903	8.809813740960152E-4
58-59	0.0	1.957736386880034E-4	0.0	1.9131652551387317	8.809813740960152E-4
60-61	0.0	1.957736386880034E-4	0.0	2.171309112218755	9.136103138773492E-4
62-63	0.0	1.957736386880034E-4	0.0	2.44441333818852	9.136103138773492E-4
64-65	0.0	1.957736386880034E-4	0.0	2.7368012675690525	9.136103138773492E-4
66-67	0.0	1.957736386880034E-4	0.0	3.075571234848752	9.136103138773492E-4
68-69	0.0	1.957736386880034E-4	0.0	3.443788820281105	9.136103138773492E-4
70-71	0.0	2.4471704836000424E-4	0.0	3.8253679565539143	9.136103138773492E-4
72-73	0.0	2.9366045803200507E-4	0.0	4.222086920885262	9.78868193440017E-4
74-75	0.0	2.9366045803200507E-4	0.0	4.640047325014258	9.78868193440017E-4
76-77	0.0	2.9366045803200507E-4	0.0	5.112775404566225	9.78868193440017E-4
78-79	0.0	2.9366045803200507E-4	0.0	5.634316378031066	9.78868193440017E-4
80-81	0.0	3.2628939781333897E-4	0.0	6.175238941726018	9.78868193440017E-4
82-83	0.0	3.2628939781333897E-4	0.0	6.736440391495071	9.951826633306838E-4
84-85	0.0	3.2628939781333897E-4	0.0	7.323908137788097	0.0010114971332213507
86-87	0.0	3.2628939781333897E-4	0.0	7.957741607510398	0.0010441260730026847
88-89	0.0	3.2628939781333897E-4	0.0	8.642035732604533	0.0010767550127840186
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2260	0.0	52.90952	1
GTATCAA	4535	0.0	42.689846	1
TCAACGC	5490	0.0	34.489895	4
CAACGCA	5705	0.0	33.022118	5
ATCAACG	5775	0.0	32.785656	3
TATCAAC	5995	0.0	31.741856	2
GTACATG	10025	0.0	30.576647	1
AACGCAG	6160	0.0	30.5745	6
TACATGG	10120	0.0	29.35425	2
ACATGGG	10795	0.0	27.298067	3
CATGGGG	8785	0.0	24.956953	4
ACGCAGA	7395	0.0	24.895103	7
CGCAGAG	7625	0.0	23.460438	8
ATGGGGG	5575	0.0	21.875517	5
GAGTACA	7405	0.0	19.800453	1
GCAGAGT	8775	0.0	19.358763	9
AGTACAT	7540	0.0	18.251719	2
AGAGTAC	7515	0.0	18.216827	10-11
TATCTCG	370	2.7866918E-9	16.67292	5
GTGGTAT	1420	0.0	16.373798	1
>>END_MODULE