FastQCFastQC Report
Mon 15 Aug 2016
SRR2921417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064764
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.051.144021
GTATCAA46300.041.475621
TCAACGC57350.032.521514
CAACGCA59000.031.6714535
ATCAACG61400.030.4536573
AACGCAG63550.029.5515736
TATCAAC64200.029.4950732
GTACATG96850.029.2999741
TACATGG102150.027.2251832
ACATGGG105800.026.1513733
CATGGGG84700.024.0372894
ACGCAGA79350.023.3078257
TATACCG2354.603862E-922.2562645
CGCAGAG82700.022.0762218
ATGGGGG52500.020.9208895
GCAGAGT94100.018.744919
TATATAC10200.017.680523
AGAGTAC82400.017.65603810-11
GTATTAG9250.017.4714811
GACCGTA1650.001455436317.288867
Produced by FastQC (version 0.11.5)