##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3064764
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33101537345127	33.0	31.0	34.0	28.0	34.0
2	31.4565924162513	34.0	31.0	34.0	28.0	34.0
3	31.534711645007576	34.0	31.0	34.0	28.0	34.0
4	35.02516474351695	37.0	35.0	37.0	32.0	37.0
5	34.75602982807159	37.0	35.0	37.0	32.0	37.0
6	34.70742543308392	37.0	35.0	37.0	32.0	37.0
7	34.570464479483576	37.0	35.0	37.0	32.0	37.0
8	34.530058758194755	37.0	35.0	37.0	31.0	37.0
9	36.1415035545967	39.0	37.0	39.0	32.0	39.0
10-11	35.98988095005031	39.0	37.0	39.0	31.0	39.0
12-13	35.9175425905551	39.0	37.0	39.0	31.0	39.0
14-15	37.14313255441529	40.0	38.0	41.0	31.0	41.0
16-17	37.115084880923945	40.0	38.0	41.0	31.0	41.0
18-19	37.07941622911258	40.0	38.0	41.0	31.0	41.0
20-21	37.03766015915092	40.0	38.0	41.0	31.0	41.0
22-23	36.92298069280375	40.0	37.0	41.0	30.0	41.0
24-25	36.851812243944394	40.0	37.0	41.0	30.0	41.0
26-27	36.711178250592866	40.0	37.0	41.0	30.0	41.0
28-29	36.55596727839403	40.0	37.0	41.0	30.0	41.0
30-31	36.41471659808063	40.0	36.5	41.0	30.0	41.0
32-33	36.35942457559538	40.0	36.5	41.0	29.5	41.0
34-35	36.48975924410493	40.0	37.0	41.0	30.0	41.0
36-37	36.45951580611101	40.0	37.0	41.0	30.0	41.0
38-39	36.36900867407735	40.0	37.0	41.0	29.5	41.0
40-41	36.23972449428406	40.0	36.0	41.0	29.0	41.0
42-43	36.13068820307208	40.0	36.0	41.0	28.5	41.0
44-45	35.98992255194854	40.0	36.0	41.0	28.0	41.0
46-47	35.827595697417486	40.0	35.5	41.0	27.5	41.0
48-49	35.66495495248574	40.0	35.0	41.0	27.0	41.0
50-51	35.50345165239477	39.5	35.0	41.0	26.5	41.0
52-53	35.32662547589308	39.0	35.0	41.0	26.0	41.0
54-55	35.11472596258635	39.0	35.0	41.0	26.0	41.0
56-57	34.847078763650316	39.0	35.0	41.0	25.0	41.0
58-59	34.62386271830392	38.0	34.5	40.0	25.0	41.0
60-61	34.34420301204269	38.0	34.0	40.0	24.0	41.0
62-63	34.04297035595563	37.0	34.0	40.0	23.0	41.0
64-65	33.73582386767791	37.0	34.0	40.0	23.0	41.0
66-67	33.40233424172301	36.0	34.0	39.0	22.0	41.0
68-69	33.037539758363124	36.0	33.0	39.0	22.0	41.0
70-71	32.66060567795759	35.0	33.0	38.5	20.5	40.0
72-73	32.26678155316364	35.0	33.0	37.5	20.0	40.0
74-75	31.865041810723437	35.0	32.5	37.0	18.5	39.0
76-77	29.1093767741986	33.0	28.5	34.5	11.0	36.5
78-79	30.772491291335974	34.5	31.5	36.0	12.5	37.0
80-81	30.970520894920455	35.0	32.0	35.5	13.5	37.0
82-83	30.813772936513217	35.0	32.0	35.0	11.0	37.0
84-85	30.583263344257503	35.0	32.0	35.0	7.5	36.0
86-87	30.343974772608917	35.0	32.0	35.0	4.0	36.0
88-89	30.06474413690581	35.0	31.0	35.0	2.0	36.0
90-91	29.881169316789155	34.0	31.0	35.0	2.0	35.0
92-93	29.735010101919755	34.0	31.0	35.0	2.0	35.0
94-95	29.631977535627538	34.0	31.0	35.0	2.0	35.0
96-97	29.4377912948599	34.0	31.0	35.0	2.0	35.0
98-99	29.16904025889106	34.0	31.0	35.0	2.0	35.0
100-101	28.078194601607173	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61862.0
3	20742.0
4	11276.0
5	7970.0
6	6953.0
7	7007.0
8	7155.0
9	7837.0
10	8424.0
11	9216.0
12	9868.0
13	10777.0
14	11400.0
15	12586.0
16	13768.0
17	14645.0
18	13647.0
19	12344.0
20	11512.0
21	11273.0
22	12210.0
23	13599.0
24	15718.0
25	19276.0
26	22861.0
27	27685.0
28	33267.0
29	39622.0
30	48793.0
31	60203.0
32	76695.0
33	101766.0
34	147968.0
35	220984.0
36	381101.0
37	725294.0
38	751695.0
39	95762.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.292742217198544	19.34917490066751	13.156820667562693	26.201262214571248
2	15.241559512808866	20.830984040470486	37.49409413877534	26.43336230794531
3	18.182364006872056	24.37452379359685	28.986404358175736	28.456707841355357
4	13.205093106372647	16.35240730232496	35.09702530140021	35.345474289902185
5	14.210596400850406	37.438551462721605	34.059053260077036	14.291798876350953
6	33.97906890086605	35.09796320840846	16.71789480416168	14.205073086563806
7	29.31192001983842	31.75485192967802	20.5684295018207	18.364798548662865
8	27.187279138107574	34.88624327747792	19.490794851564495	18.435682732850022
9	27.114699281706052	15.315871183471996	19.0438605020553	38.525569032766654
10-11	26.019090092386744	25.233089907191914	27.378361371950753	21.369458628470586
12-13	26.330468234436168	22.500670933997224	27.398921448108048	23.76993938345856
14-15	23.53857047528458	24.073357404020445	24.649112359422237	27.738959761272735
16-17	23.83124769150251	27.245164717413807	25.379327739427897	23.544259851655788
18-19	23.84475305701335	26.708994125470348	26.632446432929736	22.81380638458657
20-21	24.29155887474286	25.785911004834176	26.690131412474045	23.232398707948914
22-23	24.207273372141227	25.501145360909007	26.35778820130617	23.933793065643595
24-25	23.907272321481013	25.722236579180585	26.455975039775296	23.914516059563105
26-27	23.705235377784682	26.020477252192652	26.447358589133117	23.82692878088955
28-29	23.78192626857884	26.369621687289925	26.181600086016132	23.666851958115103
30-31	24.27284871647223	25.94912098952132	26.058674016759948	23.719356277246504
32-33	23.96679219372776	25.97721585123028	26.01815532819379	24.037836626848172
34-35	24.188470535753765	26.009701461974327	26.210345408791035	23.591482593480876
36-37	24.144814100236136	25.947412229997752	26.2544200280415	23.65335364172461
38-39	24.074832698780472	26.058628148761574	26.15930498883027	23.70723416362768
40-41	24.257040312915123	25.844767393863016	26.139226607313642	23.758965685908215
42-43	24.046626848323243	25.83270911523754	25.946088114523068	24.17457592191615
44-45	23.870055736689054	25.881510465741787	26.14997201010214	24.098461787467016
46-47	23.999032778654954	25.853363174483107	25.972666859958654	24.17493718690329
48-49	23.831351395802777	26.02392944616756	26.047262034924806	24.09745712310486
50-51	24.006448529804057	26.019354399770673	26.016825500571123	23.957371569854153
52-53	24.200568471590614	25.86106870179192	25.953344999476297	23.985017827141174
54-55	23.61659327804851	25.757920342215556	26.295797189642823	24.329689190093106
56-57	23.76140328665261	25.840776720571878	26.041179582043462	24.35664041073205
58-59	23.77877512115046	25.81628595702134	26.208653392286347	24.19628552954185
60-61	23.962265597811342	25.864224612986455	26.19737580822082	23.97613398098139
62-63	23.840872762304834	25.812821739558945	26.182477525959452	24.16382797217677
64-65	23.554492933381205	25.65174462251526	26.21485458759017	24.578907856513364
66-67	23.634113776019223	25.85011642255766	25.997311354573643	24.518458446849476
68-69	23.605099227158888	25.77608446861727	26.113361017599967	24.50545528662387
70-71	23.57096458299331	25.59875958870573	26.074049290027745	24.756226538273218
72-73	23.549606629698296	25.90803338348068	25.8733724753551	24.66898751146592
74-75	23.4589677349238	25.945187180392224	25.88317692879216	24.712668155891816
76-77	23.44509148829352	25.602870977712143	25.993779221225218	24.958258312769114
78-79	23.405515873171357	25.631740073635527	26.05942496894848	24.903319084244636
80-81	23.58433280641857	25.82781770446676	25.748290791658423	24.839558697456248
82-83	23.529274503388795	25.815264792678537	25.833198031749504	24.822262672183165
84-85	23.470368936652722	25.866218113714506	25.738189908015528	24.925223041617244
86-87	23.243601302679714	25.993574117257502	25.758979434758743	25.003845145304044
88-89	23.20744745149504	25.67875373745777	25.878526467964804	25.23527234308239
90-91	23.25959336913134	25.58751663199792	25.927734071333063	25.225155927537678
92-93	23.340061063743136	25.528105073383404	25.913226152127233	25.21860771074622
94-95	23.167743827000866	25.525983430553218	25.905933091465116	25.4003396509808
96-97	23.032539334202443	25.355406933392505	25.998639231573744	25.61341450083131
98-99	22.78499640342172	25.342980113834273	25.982027386854618	25.889996095889394
100-101	22.795126998426937	25.455873173529874	25.92309321266555	25.825906615377637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	52.0
2	73.5
3	124.5
4	110.0
5	107.5
6	194.5
7	387.0
8	573.5
9	617.0
10	538.0
11	449.0
12	420.5
13	462.0
14	529.5
15	562.5
16	640.0
17	731.5
18	844.0
19	942.5
20	1022.5
21	1183.0
22	1404.5
23	1692.5
24	2029.0
25	2537.0
26	3473.0
27	4874.0
28	6805.5
29	9741.0
30	13661.0
31	18847.0
32	26116.5
33	32145.0
34	37251.5
35	44343.0
36	53108.5
37	62011.0
38	69743.5
39	76838.5
40	82798.5
41	88360.5
42	94205.0
43	100733.5
44	107827.5
45	115165.0
46	121487.5
47	126585.0
48	131695.0
49	135084.0
50	135484.0
51	135273.0
52	133633.5
53	131115.0
54	128332.0
55	122283.0
56	113086.0
57	101280.5
58	90645.5
59	79731.5
60	66473.0
61	55122.0
62	45733.0
63	35618.0
64	26032.5
65	19450.5
66	14737.0
67	11235.5
68	8814.0
69	6980.0
70	5510.0
71	4209.5
72	3223.5
73	2618.5
74	2135.5
75	1626.0
76	1171.0
77	754.0
78	442.0
79	289.5
80	200.5
81	121.5
82	69.0
83	42.0
84	29.5
85	21.0
86	22.0
87	19.0
88	11.5
89	6.0
90	2.5
91	2.5
92	3.5
93	5.5
94	5.5
95	1.5
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1496689467769786
2	3.915472773760067E-4
3	0.005840580220858768
4	0.004926969906981419
5	0.02669047274113113
6	0.0013704154708160236
7	1.3051575912533558E-4
8	0.0021535100255680372
9	6.525787956266779E-5
10-11	0.10191649340699643
12-13	2.120881085786703E-4
14-15	0.005840580220858768
16-17	0.0
18-19	2.6103151825067117E-4
20-21	7.994090246426805E-4
22-23	3.09974927922672E-4
24-25	0.0015661891095040269
26-27	0.00309974927922672
28-29	0.007259939101346791
30-31	0.01613501072186961
32-33	0.015808721324056273
34-35	0.012415311586797548
36-37	6.852077354080118E-4
38-39	2.4471704836000424E-4
40-41	0.005318517184357425
42-43	0.008597725632381481
44-45	0.00866298351194415
46-47	0.010294430501010844
48-49	0.012415311586797548
50-51	0.006183184088562774
52-53	0.0017945916879733644
54-55	9.46239253658683E-4
56-57	0.006101611739109439
58-59	0.009446078066696163
60-61	0.007814631077629468
62-63	0.01833746415710965
64-65	0.03471719192733927
66-67	0.037180676880829974
68-69	0.02641312675298979
70-71	0.04124297988360605
72-73	0.02589106371648845
74-75	0.024618535065016423
76-77	0.2371797632705161
78-79	0.06891232081817719
80-81	0.05383775063920093
82-83	0.020278886074099018
84-85	0.03217213462439522
86-87	0.08029982080186272
88-89	0.2623529903118152
90-91	0.3151629293479041
92-93	0.05127637886636622
94-95	0.00481276861774675
96-97	0.010408631790245513
98-99	0.0014846167600506923
100-101	0.0013377865310346896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3064764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.19369667247847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87428196626787	47.47734603937068
2	11.87059411118103	14.2906988250108
3	3.8243606631756326	6.906072171760578
4	1.6809892465041838	4.04739827255084
5	0.9102344232974547	2.7395187388407685
6	0.5988954269087526	2.1629837799527976
7	0.3930296601878601	1.6560535704044737
8	0.29448599368422684	1.418096046249741
9	0.24406885678057896	1.3222266059023886
>10	1.2301142853508185	13.218147635615175
>50	0.06124706800404746	2.482776060064085
>100	0.01639417426604107	1.6193798080631971
>500	9.780932940746524E-4	0.4132374415474057
>1k	3.2603109748715193E-4	0.24606500466708195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.525787956266779E-5	0.0	0.0	3.2628939781333896E-5
6	0.0	6.525787956266779E-5	0.0	0.0	3.2628939781333896E-5
7	0.0	6.525787956266779E-5	0.0	0.0	3.2628939781333896E-5
8	0.0	6.525787956266779E-5	0.0	0.0	3.2628939781333896E-5
9	0.0	6.525787956266779E-5	0.0	6.525787956266779E-5	3.2628939781333896E-5
10-11	0.0	6.525787956266779E-5	0.0	6.525787956266779E-5	3.2628939781333896E-5
12-13	0.0	6.525787956266779E-5	0.0	6.525787956266779E-5	1.1420128923466863E-4
14-15	0.0	6.525787956266779E-5	0.0	6.525787956266779E-5	1.957736386880034E-4
16-17	0.0	6.525787956266779E-5	0.0	6.525787956266779E-5	2.284025784693373E-4
18-19	0.0	6.525787956266779E-5	0.0	6.525787956266779E-5	2.284025784693373E-4
20-21	0.0	6.525787956266779E-5	0.0	1.3051575912533558E-4	2.4471704836000424E-4
22-23	0.0	6.525787956266779E-5	0.0	2.284025784693373E-4	2.9366045803200507E-4
24-25	0.0	6.525787956266779E-5	0.0	4.731196268293415E-4	3.2628939781333897E-4
26-27	0.0	6.525787956266779E-5	0.0	0.0010604405428933515	3.2628939781333897E-4
28-29	0.0	6.525787956266779E-5	0.0	0.004143875352229405	3.7523280748533985E-4
30-31	0.0	6.525787956266779E-5	0.0	0.017619627481920305	3.915472773760068E-4
32-33	0.0	6.525787956266779E-5	0.0	0.04667569835719814	3.915472773760068E-4
34-35	0.0	8.157234945333474E-5	0.0	0.08584674056468948	3.915472773760068E-4
36-37	0.0	9.78868193440017E-5	0.0	0.14417097042382382	3.915472773760068E-4
38-39	0.0	9.78868193440017E-5	0.0	0.23760393948767344	3.915472773760068E-4
40-41	0.0	9.78868193440017E-5	0.0	0.3435664214275552	3.915472773760068E-4
42-43	0.0	1.3051575912533558E-4	0.0	0.4549616218410292	3.915472773760068E-4
44-45	0.0	1.4683022901600253E-4	0.0	0.5924762885494609	3.915472773760068E-4
46-47	0.0	1.6314469890666949E-4	0.0	0.7455386450636983	4.241762171573407E-4
48-49	0.0	1.6314469890666949E-4	0.0	0.9006403103142688	4.241762171573407E-4
50-51	0.0	1.6314469890666949E-4	0.0	1.0666400414518051	4.241762171573407E-4
52-53	0.0	1.6314469890666949E-4	0.0	1.2436520397655415	5.383775063920093E-4
54-55	0.0	1.957736386880034E-4	0.0	1.4514494427629665	6.036353859546771E-4
56-57	0.0	1.957736386880034E-4	0.0	1.684436387271581	6.852077354080118E-4
58-59	0.0	2.284025784693373E-4	0.0	1.9236065158687585	7.015222052986788E-4
60-61	0.0	2.284025784693373E-4	0.0	2.1815709137799844	7.178366751893457E-4
62-63	0.0	2.284025784693373E-4	0.0	2.4554582343045013	7.504656149706796E-4
64-65	0.0	2.284025784693373E-4	0.0	2.7504075354578688	7.504656149706796E-4
66-67	0.0	2.9366045803200507E-4	0.0	3.0906294905578373	7.667800848613466E-4
68-69	0.0	2.9366045803200507E-4	0.0	3.4597770007739586	7.830945547520136E-4
70-71	0.0	3.0997492792267205E-4	0.0	3.843477018132554	7.830945547520136E-4
72-73	0.0	3.2628939781333897E-4	0.0	4.2428878699958625	9.136103138773491E-4
74-75	0.0	3.2628939781333897E-4	0.0	4.664992149477088	9.78868193440017E-4
76-77	0.0	3.2628939781333897E-4	0.0	5.1418477899113935	0.0010114971332213507
78-79	0.0	3.2628939781333897E-4	0.0	5.669425117235781	0.0010930694826746857
80-81	0.0	3.2628939781333897E-4	0.0	6.216710324188094	0.0011420128923466865
82-83	0.0	3.2628939781333897E-4	0.0	6.785269599877838	0.0011746418321280203
84-85	0.0	3.2628939781333897E-4	0.0	7.379099989428223	0.0011746418321280203
86-87	0.0	3.2628939781333897E-4	0.0	8.022053247819407	0.0012398997116906882
88-89	0.0	3.2628939781333897E-4	0.0	8.716625488944663	0.0012398997116906882
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2435	0.0	51.14402	1
GTATCAA	4630	0.0	41.47562	1
TCAACGC	5735	0.0	32.52151	4
CAACGCA	5900	0.0	31.671453	5
ATCAACG	6140	0.0	30.453657	3
AACGCAG	6355	0.0	29.551573	6
TATCAAC	6420	0.0	29.495073	2
GTACATG	9685	0.0	29.299974	1
TACATGG	10215	0.0	27.225183	2
ACATGGG	10580	0.0	26.151373	3
CATGGGG	8470	0.0	24.037289	4
ACGCAGA	7935	0.0	23.307825	7
TATACCG	235	4.603862E-9	22.256264	5
CGCAGAG	8270	0.0	22.076221	8
ATGGGGG	5250	0.0	20.920889	5
GCAGAGT	9410	0.0	18.74491	9
TATATAC	1020	0.0	17.68052	3
AGAGTAC	8240	0.0	17.656038	10-11
GTATTAG	925	0.0	17.471481	1
GACCGTA	165	0.0014554363	17.28886	7
>>END_MODULE