FastQCFastQC Report
Mon 15 Aug 2016
SRR2921416_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921416_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773696
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.055.9630051
GTATCAA40300.039.5655561
TCAACGC51650.030.7826864
TATCAAC52600.030.3152452
ATCAACG52950.030.0252323
CAACGCA53400.029.6808285
AACGCAG56950.027.6577666
GTGGTAT11350.027.1769391
ACGCAGA66400.023.295467
GTACATG67300.022.705121
CGCAGAG67850.022.1001788
TACATGG69950.021.5069622
TGGTATC13700.021.477252
ACATGGG70450.020.0070483
GAGTACT29450.019.57825712-13
GCAGAGT73800.019.4825069
CAGAGTA69650.019.00876210-11
CATGGGG57550.018.7202914
GTACTTT31350.018.08818614-15
TAAGGTA4850.017.6141954
Produced by FastQC (version 0.11.5)