##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921416_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1773696
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.633327808147506	31.0	31.0	34.0	27.0	34.0
2	30.70108970195569	33.0	31.0	34.0	27.0	34.0
3	30.73247839539583	33.0	31.0	34.0	27.0	34.0
4	34.20260574529119	37.0	35.0	37.0	30.0	37.0
5	34.19542187612759	37.0	35.0	37.0	30.0	37.0
6	34.24265093905607	37.0	35.0	37.0	30.0	37.0
7	34.16917329688966	37.0	35.0	37.0	30.0	37.0
8	34.134471747131414	37.0	35.0	37.0	30.0	37.0
9	35.72007097044815	39.0	37.0	39.0	30.0	39.0
10-11	35.62305772804359	39.0	37.0	39.0	30.0	39.0
12-13	35.51450981453417	39.0	37.0	39.0	30.0	39.0
14-15	36.66854974020351	40.0	37.0	41.0	30.0	41.0
16-17	36.611946466587284	40.0	37.0	41.0	30.0	41.0
18-19	36.57150802617811	40.0	37.0	41.0	30.0	41.0
20-21	36.51089645576243	40.0	37.0	41.0	29.0	41.0
22-23	36.39404525916504	40.0	37.0	41.0	29.0	41.0
24-25	36.2926231439886	40.0	37.0	41.0	28.5	41.0
26-27	36.14132297755647	40.0	36.0	41.0	27.5	41.0
28-29	35.99807858843906	40.0	36.0	41.0	27.0	41.0
30-31	35.83962781671718	40.0	36.0	41.0	27.0	41.0
32-33	35.645269256963985	40.0	35.5	41.0	26.5	41.0
34-35	35.470019665151185	39.5	35.0	41.0	25.5	41.0
36-37	35.29012356119651	39.0	35.0	41.0	25.0	41.0
38-39	35.51604305360107	39.5	35.0	41.0	26.0	41.0
40-41	35.62504031130476	40.0	35.0	41.0	26.0	41.0
42-43	35.44479493667461	40.0	35.0	41.0	25.0	41.0
44-45	35.300833119091436	40.0	35.0	41.0	24.0	41.0
46-47	35.1128902585336	40.0	35.0	41.0	23.5	41.0
48-49	34.93962916982392	39.5	35.0	41.0	23.0	41.0
50-51	33.20684604351591	37.0	32.5	39.5	18.5	40.0
52-53	33.36207219275457	37.5	33.5	39.5	19.5	40.0
54-55	34.21121460498304	39.0	34.0	40.5	20.5	41.0
56-57	34.205376795121595	39.0	34.0	41.0	20.0	41.0
58-59	34.016482813289315	38.0	34.0	41.0	19.5	41.0
60-61	33.764181404254174	38.0	34.0	40.0	18.0	41.0
62-63	33.46740873295086	37.0	34.0	40.0	17.0	41.0
64-65	33.16878653388179	37.0	34.0	40.0	13.5	41.0
66-67	32.81852160685935	36.0	33.0	39.0	11.5	41.0
68-69	32.444430443548384	35.5	33.0	39.0	7.5	41.0
70-71	32.06622386248827	35.0	33.0	38.5	6.5	40.5
72-73	31.714047672205382	35.0	32.5	37.0	2.0	40.0
74-75	31.320667972414665	35.0	32.0	37.0	2.0	39.0
76-77	30.949124314425923	35.0	32.0	36.5	2.0	39.0
78-79	30.617407379844124	35.0	32.0	36.0	2.0	38.0
80-81	30.282568997167495	35.0	31.5	35.5	2.0	37.0
82-83	29.995537566753264	35.0	31.0	35.0	2.0	37.0
84-85	29.776549081691563	35.0	31.0	35.0	2.0	36.0
86-87	29.56332370372375	35.0	31.0	35.0	2.0	36.0
88-89	29.369935998051524	35.0	31.0	35.0	2.0	36.0
90-91	29.11757398111063	35.0	31.0	35.0	2.0	35.5
92-93	28.931367043732408	35.0	30.5	35.0	2.0	35.0
94-95	28.734706229252364	34.0	30.0	35.0	2.0	35.0
96-97	28.485644947048424	34.0	30.0	35.0	2.0	35.0
98-99	28.258420552338166	34.0	30.0	35.0	2.0	35.0
100-101	27.22773547439922	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55558.0
3	9582.0
4	6712.0
5	5128.0
6	4828.0
7	4779.0
8	5145.0
9	5548.0
10	6123.0
11	6408.0
12	6567.0
13	6701.0
14	7326.0
15	8233.0
16	8729.0
17	9745.0
18	8565.0
19	7316.0
20	7119.0
21	7654.0
22	8318.0
23	9270.0
24	11062.0
25	12889.0
26	15386.0
27	17888.0
28	21489.0
29	25537.0
30	30523.0
31	37264.0
32	46407.0
33	60957.0
34	87278.0
35	128184.0
36	214406.0
37	388527.0
38	400622.0
39	69914.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66463025954319	18.40855629452878	13.810732598872924	24.11608084705511
2	17.101305213180307	19.84283960590866	37.80040889458553	25.2554462863255
3	19.59881992587871	23.521607427923534	29.17987104926189	27.699701596935867
4	14.416006201015112	16.255268352426615	34.81831623710308	34.5104092094552
5	15.381547603266533	36.33754464066626	33.089796910705125	15.19111084536208
6	33.93857678409506	33.54660368931776	17.072681093712642	15.442138432874536
7	29.218168489906393	31.57730912371715	20.43024698319612	18.774275403180333
8	27.3743665742962	33.168498850162464	19.77534464110294	19.681789934438395
9	27.292649187638553	15.53380812017457	19.379209344003538	37.79433334818334
10-11	26.214429920034288	25.030452387128793	26.841467128339897	21.91365056449703
12-13	26.678122693027184	22.83571054339802	26.660498760121072	23.825668003453725
14-15	24.378381381632924	24.332833291451653	24.26596361849822	27.022821708417204
16-17	24.044672289475987	26.97563919272926	25.388647790495366	23.591040727299387
18-19	24.01391457150124	26.259792054020615	26.807318092382964	22.918975282095182
20-21	24.750907616932782	25.22031652039054	26.808163265306124	23.220612597370554
22-23	24.81914913719846	24.97483501386648	26.343883589834277	23.862132259100786
24-25	24.42998469731078	25.407004717154074	26.21887075968009	23.944139825855057
26-27	24.00799050028326	25.631638952109732	26.42321621584277	23.93715433176424
28-29	24.115743840975576	25.848997286152326	26.31826031790787	23.716998554964228
30-31	24.6271282489364	25.360353883499254	26.13629251211611	23.876225355448234
32-33	24.272562884792457	25.466346324627676	25.96901869219479	24.292072098385077
34-35	24.636395340018883	25.64884144668828	25.987545153681097	23.72721805961174
36-37	24.536481845762133	25.546933098339426	26.10118156605849	23.815403489839955
38-39	24.411850441848674	25.564553096549282	26.140284206270852	23.883312255331195
40-41	24.541940940164917	25.41692860666714	26.166466981464513	23.874663471703432
42-43	24.383180202550196	25.454102154303055	25.882939918922286	24.27977772422446
44-45	24.291207067042397	25.499655273802418	26.00334247425004	24.205795184905142
46-47	24.462885646001805	25.488177284104196	25.777110783639962	24.271826286254036
48-49	24.211342165901872	25.693046682533126	25.823936513065537	24.27167463849946
50-51	24.271354896183837	25.62050885751238	26.00554563682886	24.102590609474923
52-53	24.522345078687614	25.462783153238526	25.909275153131194	24.105596614942666
54-55	24.098798379007885	25.363238888444794	26.06416799023586	24.47379474231146
56-57	24.146331886105454	25.522342480841814	25.868569786865116	24.462755846187616
58-59	24.1853236219992	25.47035979091856	26.041085225770438	24.3032313613118
60-61	24.282231940646863	25.34895789340763	26.25469046133263	24.114119704612875
62-63	24.24555562366752	25.372585787882958	26.255175606927043	24.126682981522478
64-65	24.080795320104365	25.264102908889157	26.142204825662134	24.51289694534434
66-67	24.069221467512964	25.459446954754263	25.907094025374768	24.564237552358016
68-69	24.09726457162242	25.344749617478406	26.100273901562087	24.457711909337085
70-71	23.983126333346704	25.25531630076178	26.0816311775926	24.679926188298914
72-73	23.916621760089292	25.523542908658325	25.951172146398832	24.608663184853548
74-75	24.01098094151406	25.596552074742707	25.947727555158895	24.444739428584338
76-77	24.02010319905782	25.200933140905974	25.934341223420027	24.844622436616177
78-79	23.909133849903778	25.284906643282756	26.05298218295272	24.75297732386074
80-81	24.073391820465677	25.455350917143484	25.84953444980997	24.621722812580874
82-83	23.99564799869813	25.39555493525819	26.049343964903727	24.559453101139955
84-85	23.947229172781856	25.483892201646306	25.962805143850453	24.606073481721385
86-87	23.787268887546418	25.72805685548609	25.835655501235067	24.649018755732424
88-89	23.766074911567326	25.416931208858212	25.975231786994158	24.841762092580304
90-91	23.901448441631867	25.223957054495344	26.016258554587317	24.858335949285472
92-93	23.95865062980819	25.0983183451662	26.141433743975018	24.801597281050597
94-95	23.808892678188865	25.035303320306596	26.23596945588095	24.91983454562359
96-97	23.806232206875855	24.917269214313286	26.29329907482819	24.983199503982664
98-99	23.616270362678197	24.924681049463825	26.146399355741114	25.31264923211687
100-101	23.856790416521832	24.818783752850713	26.26257916288691	25.06184666774054
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	58.5
2	64.0
3	79.5
4	76.5
5	84.0
6	143.0
7	293.5
8	459.5
9	557.0
10	509.0
11	450.0
12	449.0
13	470.0
14	564.0
15	625.5
16	692.0
17	801.5
18	893.5
19	1019.5
20	1152.5
21	1255.5
22	1346.5
23	1516.0
24	1736.0
25	1977.5
26	2469.5
27	3210.5
28	4102.0
29	5491.5
30	7762.5
31	10568.5
32	14633.0
33	18187.5
34	20819.5
35	24406.5
36	28970.0
37	33508.0
38	37422.5
39	40922.5
40	43969.0
41	47367.0
42	50393.5
43	54164.0
44	58306.0
45	62446.0
46	66098.5
47	69551.0
48	73626.5
49	76139.0
50	76818.0
51	76921.0
52	77228.5
53	77127.0
54	76673.5
55	74144.5
56	69070.0
57	62720.0
58	56503.0
59	49824.0
60	41910.0
61	35202.5
62	29573.0
63	23279.0
64	17153.5
65	12870.5
66	10030.0
67	7941.0
68	6163.5
69	4758.0
70	3746.0
71	2870.0
72	2168.5
73	1694.5
74	1371.5
75	1072.5
76	841.0
77	603.5
78	408.5
79	281.0
80	190.0
81	146.0
82	103.0
83	74.5
84	67.5
85	60.0
86	45.5
87	32.5
88	23.5
89	23.0
90	23.0
91	19.5
92	18.0
93	16.0
94	11.5
95	8.0
96	6.5
97	5.5
98	3.5
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05446254600562893
2	0.06269394529840513
3	0.05220736811719709
4	0.06105894132929205
5	0.05237650645882947
6	0.020296600995886555
7	0.016688316374395613
8	0.022664537778739988
9	0.02305919390921556
10-11	0.02458143898390705
12-13	0.03038852204661904
14-15	0.04761244316951721
16-17	0.03162886988525655
18-19	0.025201612903225805
20-21	0.02866894890668976
22-23	0.05048779497726781
24-25	0.045385455004690774
26-27	0.03329206357797503
28-29	0.021621518005340262
30-31	0.013023652305693873
32-33	0.009866403261889298
34-35	0.00955631630222992
36-37	0.0018605217579562676
38-39	0.007047430901349499
40-41	0.004002940751966515
42-43	0.2205845872122393
44-45	0.19453728260085157
46-47	0.20102091903009311
48-49	0.19760996247383994
50-51	0.19594676878112147
52-53	0.2009081601356715
54-55	0.19989333008587715
56-57	0.2120149112361983
58-59	0.2067998123691997
60-61	0.21128197842245794
62-63	0.2105490456087176
64-65	0.21097189146279857
66-67	0.20023160676914195
68-69	0.1992731561665584
70-71	0.182951306199033
72-73	0.18909666594500973
74-75	0.18920942483943134
76-77	0.18779993865916145
78-79	0.21525672945081906
80-81	0.2233753698491737
82-83	0.22188131449808762
84-85	0.21731457927401313
86-87	0.23436936205527892
88-89	0.23281892725698206
90-91	0.23140944107671213
92-93	0.2361735043660244
94-95	0.2473084451901566
96-97	0.24823870606913473
98-99	0.23797764667676988
100-101	0.24812594717471315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1773696.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.71135880220922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41062464953531	53.49602766657593
2	10.820657176681829	14.220801724252796
3	3.172019414482019	6.253131176178048
4	1.4851623006320132	3.9036813126537897
5	0.803516588444896	2.6400083423414817
6	0.5299369143039392	2.089372483101705
7	0.36324981763452924	1.6708747370993735
8	0.26200698664785815	1.3773468086642437
9	0.20793261573656988	1.2297181247412874
>10	0.9066459332195776	9.989758595717376
>50	0.02653895710269389	1.1723144542362915
>100	0.010503657593302736	1.3178575773972763
>500	0.0010328468446604373	0.45161675852811245
>1k	1.7214114077673955E-4	0.18749023851229715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.6379447210795987E-5
2	0.0	0.0	0.0	0.0	5.6379447210795987E-5
3	0.0	0.0	0.0	0.0	5.6379447210795987E-5
4	0.0	0.0	0.0	5.6379447210795987E-5	5.6379447210795987E-5
5	0.0	0.0	0.0	5.6379447210795987E-5	5.6379447210795987E-5
6	0.0	0.0	0.0	5.6379447210795987E-5	1.6913834163238795E-4
7	0.0	0.0	0.0	5.6379447210795987E-5	1.6913834163238795E-4
8	0.0	0.0	0.0	5.6379447210795987E-5	1.6913834163238795E-4
9	0.0	0.0	0.0	5.6379447210795987E-5	1.6913834163238795E-4
10-11	0.0	0.0	0.0	5.6379447210795987E-5	1.6913834163238795E-4
12-13	0.0	0.0	0.0	5.6379447210795987E-5	1.9732806523778596E-4
14-15	0.0	0.0	0.0	8.456917081619398E-5	2.8189723605397994E-4
16-17	0.0	0.0	0.0	1.4094861802698997E-4	3.382766832647759E-4
18-19	0.0	0.0	0.0	2.2551778884318395E-4	3.946561304755719E-4
20-21	0.0	0.0	0.0	2.8189723605397994E-4	5.074150248971639E-4
22-23	0.0	0.0	0.0	3.100869596593779E-4	5.356047485025619E-4
24-25	0.0	0.0	0.0	4.2284585408096994E-4	5.637944721079599E-4
26-27	0.0	0.0	0.0	7.611225373457458E-4	6.201739193187559E-4
28-29	0.0	0.0	0.0	0.0024525059536696257	6.201739193187559E-4
30-31	0.0	0.0	0.0	0.008964332106516561	7.047430901349499E-4
32-33	0.0	0.0	0.0	0.025229802626831204	8.456917081619398E-4
34-35	0.0	0.0	0.0	0.05463168434726132	9.020711553727358E-4
36-37	0.0	0.0	0.0	0.09313884679223497	9.020711553727358E-4
38-39	0.0	0.0	0.0	0.14591000938154003	0.0010430197733997258
40-41	0.0	0.0	0.0	0.20355799415457893	0.0010712094970051237
42-43	0.0	0.0	0.0	0.27476523598181424	0.0010712094970051237
44-45	0.0	0.0	0.0	0.37275271523417763	0.0010712094970051237
46-47	0.0	0.0	0.0	0.48587807606263983	0.0011839683914267158
48-49	0.0	0.0	0.0	0.5973684329219888	0.0011839683914267158
50-51	0.0	0.0	0.0	0.7030517067186259	0.0012403478386375118
52-53	0.0	0.0	0.0	0.820349146640687	0.0012967272858483078
54-55	0.0	2.8189723605397993E-5	0.0	0.9718407212960958	0.0013531067330591036
56-57	0.0	8.456917081619398E-5	0.0	1.159612470231652	0.0013812964566645015
58-59	0.0	1.6913834163238795E-4	0.0	1.344847144042722	0.0014094861802698996
60-61	0.0	1.6913834163238795E-4	0.0	1.526811809915566	0.0014094861802698996
62-63	0.0	1.6913834163238795E-4	0.0	1.7207007288734935	0.0014094861802698996
64-65	0.0	1.6913834163238795E-4	0.0	1.9501932687450387	0.0014094861802698996
66-67	0.0	1.6913834163238795E-4	0.0	2.2339228368333695	0.0014094861802698996
68-69	0.0	1.6913834163238795E-4	0.0	2.5394712509922783	0.0014376759038752978
70-71	0.0	1.6913834163238795E-4	0.0	2.8400864635202425	0.0015504347982968898
72-73	0.0	1.6913834163238795E-4	0.0	3.1449301345890164	0.0018323320343508697
74-75	0.0	1.6913834163238795E-4	0.0	3.479598533232301	0.0018605217579562676
76-77	0.0	1.6913834163238795E-4	0.0	3.894382126362127	0.0018605217579562676
78-79	0.0	1.6913834163238795E-4	0.0	4.3729308742873645	0.0018605217579562676
80-81	0.0	1.6913834163238795E-4	0.0	4.834396649707729	0.0019169012051670636
82-83	0.0	1.6913834163238795E-4	0.0	5.29135206935123	0.0021424189940102475
84-85	0.0	1.6913834163238795E-4	0.0	5.785066888576171	0.0021424189940102475
86-87	0.0	1.6913834163238795E-4	0.0	6.35125748719059	0.0021987984412210433
88-89	0.0	1.6913834163238795E-4	0.0	6.988570758461428	0.0022551778884318395
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2510	0.0	55.963005	1
GTATCAA	4030	0.0	39.565556	1
TCAACGC	5165	0.0	30.782686	4
TATCAAC	5260	0.0	30.315245	2
ATCAACG	5295	0.0	30.025232	3
CAACGCA	5340	0.0	29.680828	5
AACGCAG	5695	0.0	27.657766	6
GTGGTAT	1135	0.0	27.176939	1
ACGCAGA	6640	0.0	23.29546	7
GTACATG	6730	0.0	22.70512	1
CGCAGAG	6785	0.0	22.100178	8
TACATGG	6995	0.0	21.506962	2
TGGTATC	1370	0.0	21.47725	2
ACATGGG	7045	0.0	20.007048	3
GAGTACT	2945	0.0	19.578257	12-13
GCAGAGT	7380	0.0	19.482506	9
CAGAGTA	6965	0.0	19.008762	10-11
CATGGGG	5755	0.0	18.720291	4
GTACTTT	3135	0.0	18.088186	14-15
TAAGGTA	485	0.0	17.614195	4
>>END_MODULE