FastQCFastQC Report
Mon 15 Aug 2016
SRR2921416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773696
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.061.8784451
GTATCAA36700.044.029911
TATCAAC50350.032.0462382
ATCAACG51100.031.2972833
TCAACGC51050.031.1420124
CAACGCA52400.030.3936925
AACGCAG55500.028.6944056
ATAAGCG1501.0526728E-625.3552935
GTACATG67000.024.9690971
ACGCAGA67100.023.1670447
TACATGG70950.023.143092
TAGGTCG1050.00218565322.6386575
GTGGTAT9300.021.9745771
ACATGGG74700.021.9177593
CGCAGAG69800.021.7941518
GTATACC2257.115341E-821.1228491
CATGGGG60800.020.137884
GCAGAGT74450.019.4112839
GAGTACT30800.019.25405512-13
GTACGTA1507.694497E-419.0105631
Produced by FastQC (version 0.11.5)