FastQCFastQC Report
Mon 15 Aug 2016
SRR2921415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709343
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.060.887531
GTATCAA50050.041.622891
TCAACGC62050.033.5783044
CAACGCA64250.032.277825
ATCAACG64400.032.2769243
TATCAAC64900.031.8100622
AACGCAG68300.030.2848456
GTACATG83950.028.4892921
TACATGG87300.026.9098282
ACGCAGA79950.025.4607127
CGCAGAG81350.024.8507758
ACATGGG91200.024.7694283
CATGGGG72550.023.0926254
CCGTACG1302.9615837E-421.9026059
GCAGAGT91650.020.91889
GAGTACA66750.019.9768431
GTGTAAG12450.019.82011
GTATAGG7950.019.6978421
CAGAGTA82550.019.51706710-11
ATGGGGG45900.019.4375295
Produced by FastQC (version 0.11.5)