##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921415_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2709343
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.500496983955152	31.0	31.0	34.0	27.0	34.0
2	30.554952621355067	33.0	31.0	34.0	26.0	34.0
3	30.6025697004772	33.0	31.0	34.0	26.0	34.0
4	34.07503959446995	37.0	35.0	37.0	30.0	37.0
5	34.0738880237755	37.0	35.0	37.0	30.0	37.0
6	34.12154754861234	37.0	35.0	37.0	30.0	37.0
7	34.038881012850716	37.0	35.0	37.0	30.0	37.0
8	34.006583883989585	37.0	35.0	37.0	30.0	37.0
9	35.56999206080589	39.0	37.0	39.0	30.0	39.0
10-11	35.46970557068633	39.0	37.0	39.0	30.0	39.0
12-13	35.36219481992498	39.0	35.0	39.0	30.0	39.0
14-15	36.4949838392555	40.0	37.0	41.0	29.0	41.0
16-17	36.44313418419152	40.0	37.0	41.0	29.0	41.0
18-19	36.40091121722129	40.0	37.0	41.0	28.5	41.0
20-21	36.34099853728377	40.0	37.0	41.0	28.0	41.0
22-23	36.22844874200129	40.0	36.5	41.0	27.5	41.0
24-25	36.12795352969336	40.0	36.0	41.0	27.0	41.0
26-27	35.9770366838012	40.0	36.0	41.0	27.0	41.0
28-29	35.833123749927566	40.0	36.0	41.0	27.0	41.0
30-31	35.68056554670265	40.0	36.0	41.0	26.5	41.0
32-33	35.485748020830144	39.5	35.0	41.0	25.5	41.0
34-35	35.32003053876899	39.0	35.0	41.0	25.0	41.0
36-37	35.13959638923532	39.0	35.0	41.0	24.5	41.0
38-39	35.35842231862116	39.5	35.0	41.0	25.0	41.0
40-41	35.47216668395253	40.0	35.0	41.0	25.0	41.0
42-43	35.293916458713426	40.0	35.0	41.0	24.0	41.0
44-45	35.1618167208803	40.0	35.0	41.0	24.0	41.0
46-47	34.99406978001678	40.0	35.0	41.0	23.0	41.0
48-49	34.83505724450541	39.0	35.0	41.0	22.5	41.0
50-51	33.10547483282848	37.0	32.5	39.5	18.5	40.0
52-53	33.261483872658424	37.5	33.0	39.5	18.5	40.0
54-55	34.12357442376252	39.0	34.0	40.5	19.5	41.0
56-57	34.137737636024674	39.0	34.0	41.0	19.0	41.0
58-59	33.95802690910675	38.0	34.0	41.0	18.0	41.0
60-61	33.70823258627645	38.0	34.0	40.0	16.0	41.0
62-63	33.41607928564231	37.0	34.0	40.0	13.5	41.0
64-65	33.12713358921333	37.0	33.0	40.0	12.5	41.0
66-67	32.789384548209654	36.0	33.0	39.5	8.0	41.0
68-69	32.420112366725064	36.0	33.0	39.0	7.0	41.0
70-71	32.0330561689679	35.0	32.5	39.0	2.0	40.5
72-73	31.675756816320416	35.0	32.0	37.5	2.0	40.0
74-75	31.274093756309185	35.0	32.0	37.0	2.0	39.0
76-77	30.895343631278877	35.0	32.0	36.5	2.0	39.0
78-79	30.54500869768058	35.0	31.0	36.0	2.0	38.5
80-81	30.187520184782805	35.0	31.0	36.0	2.0	37.0
82-83	29.878386199163412	35.0	31.0	35.0	2.0	37.0
84-85	29.634719745709567	35.0	31.0	35.0	2.0	36.5
86-87	29.403709866192653	35.0	31.0	35.0	2.0	36.0
88-89	29.186789933943395	35.0	30.5	35.0	2.0	36.0
90-91	28.91720243616257	34.5	30.0	35.0	2.0	35.5
92-93	28.70891670046945	34.0	30.0	35.0	2.0	35.0
94-95	28.487237496322912	34.0	30.0	35.0	2.0	35.0
96-97	28.221681787798737	34.0	29.0	35.0	2.0	35.0
98-99	27.973077790445874	34.0	29.0	35.0	2.0	35.0
100-101	26.908510291978537	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85859.0
3	15147.0
4	10589.0
5	8040.0
6	7411.0
7	7816.0
8	8208.0
9	8647.0
10	9445.0
11	10450.0
12	10352.0
13	10965.0
14	11811.0
15	12952.0
16	13778.0
17	14676.0
18	12985.0
19	11881.0
20	11795.0
21	12394.0
22	13503.0
23	15616.0
24	17792.0
25	21031.0
26	24813.0
27	29665.0
28	34700.0
29	41012.0
30	48609.0
31	59094.0
32	73616.0
33	95201.0
34	134006.0
35	193419.0
36	316819.0
37	571151.0
38	607853.0
39	116226.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90462322829426	18.424004020808646	12.893213334401807	24.778159416495285
2	16.142306176693154	19.95471451716967	38.96390715257278	24.939072153564396
3	19.249781752274426	23.71318777576394	29.773642765032506	27.263387706929127
4	13.646566560060272	15.671989600336808	36.2837822865964	34.39766155300652
5	14.209795725739177	37.33842643419793	34.180903309001515	14.270874531061379
6	33.333899384419446	34.90159017286093	17.202255590523567	14.562254852196062
7	28.741186459448485	32.248661818450294	20.886226881760123	18.1239248403411
8	26.930277254062123	34.34895816989651	20.15333311428954	18.567431461751816
9	27.269747018722374	15.489390262181132	19.545891850743775	37.69497086835272
10-11	25.723115476967518	25.258593064707153	27.712855189360493	21.305436268964844
12-13	26.189939967657853	22.806087412406683	27.573360555871428	23.430612064064036
14-15	23.424063318848244	24.4770264601532	24.900888898079522	27.198021322919036
16-17	23.40752714056383	27.551105929055726	25.916174018605187	23.12519291177526
18-19	23.448026344935542	26.84021368341775	27.309335174328453	22.40242479731825
20-21	23.808948033854872	25.975134771722924	27.434285690024023	22.781631504398185
22-23	23.87732862429343	25.721911843612016	27.014792928906868	23.385966603187686
24-25	23.540078939701488	26.03963306221274	27.01845877414893	23.401829223936847
26-27	23.214538228736263	26.25126778077537	27.09777797304976	23.436416017438606
28-29	23.34884955503921	26.650928942866486	26.82486346241737	23.175358039676933
30-31	23.825915418401706	26.135057171602664	26.717782822291746	23.32124458770388
32-33	23.51331009290266	26.165140682861143	26.596247718776024	23.725301505460173
34-35	23.864830575887318	26.300446386220607	26.65800200731685	23.176721030575223
36-37	23.831966804685198	26.167553366222542	26.76660153294322	23.233878296149037
38-39	23.619279426328657	26.380997413577038	26.712503972652645	23.287219187441657
40-41	23.843816543181713	26.09950698296236	26.73557167571534	23.32110479814058
42-43	23.616833730787995	26.080590267335886	26.487958177930228	23.814617823945895
44-45	23.41126580090102	26.165044010935013	26.740121461120513	23.68356872704345
46-47	23.68482170527728	26.186924141440514	26.357707786151362	23.770546367130844
48-49	23.385951096156653	26.42295672639102	26.409569456907693	23.781522720544633
50-51	23.599540476168904	26.30953290064526	26.62483148296654	23.466095140219302
52-53	23.85987445587344	26.117242688997127	26.526434366701494	23.49644848842793
54-55	23.17013030022472	26.04236758949484	26.852216020971703	23.935286089308732
56-57	23.30930613941715	26.224136770446293	26.55795190517438	23.908605184962177
58-59	23.42465824354082	26.109372422633705	26.680858901423388	23.785110432402085
60-61	23.46292438410732	26.113220876225984	26.870702066336932	23.553152673329762
62-63	23.450174347988824	26.039153217633924	26.95453563666974	23.556136797707513
64-65	23.17394042886219	25.884185683709237	26.864727958372033	24.07714592905655
66-67	23.240976449336664	26.051623480064784	26.637750476466255	24.069649594132294
68-69	23.294704827724953	25.948744253060596	26.815999568055005	23.94055135115945
70-71	23.2625040234472	25.805692412846266	26.734047967810206	24.197755595896332
72-73	23.222737686468026	26.04869630235808	26.560723572021157	24.167842439152736
74-75	23.203219208754163	26.13636153530698	26.643707888311326	24.01671136762753
76-77	23.26478450911327	25.72062793476599	26.68158844044476	24.332999115675978
78-79	23.207656072952247	25.80969336982499	26.733950244328785	24.248700312893973
80-81	23.45686182070321	25.93008870458678	26.498370669799993	24.11467880491002
82-83	23.427830046477734	25.930470196360574	26.633295665345514	24.008404091816182
84-85	23.36743327578602	25.958763820833358	26.569326326554954	24.10447657682566
86-87	23.212533431442676	26.158958220847012	26.506077359512105	24.12243098819821
88-89	23.189210555862505	25.859158858261562	26.55698121081038	24.39464937506555
90-91	23.35308806392542	25.63069012078501	26.68331391154764	24.33290790374193
92-93	23.558549868010235	25.44506660160437	26.780076805816243	24.216306724569147
94-95	23.46190084260678	25.35425577750904	26.834751429427172	24.34909195045701
96-97	23.39563790888245	25.14654232268078	26.860261940065875	24.59755782837089
98-99	23.181801618058984	25.219848589752292	26.78851898417845	24.809830808010275
100-101	23.52789261453938	25.14043569335597	26.83280488998333	24.49886680212132
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	116.0
2	138.5
3	199.5
4	191.0
5	229.5
6	334.5
7	589.0
8	923.5
9	1054.0
10	930.0
11	774.5
12	781.0
13	833.5
14	912.5
15	1056.0
16	1181.5
17	1307.0
18	1436.5
19	1597.5
20	1778.5
21	1934.5
22	2155.0
23	2527.0
24	2961.0
25	3601.0
26	4663.0
27	6158.5
28	8291.5
29	11452.0
30	15747.0
31	21557.5
32	29821.5
33	35555.0
34	39205.5
35	45123.5
36	53180.5
37	60962.0
38	67441.0
39	73450.5
40	77551.5
41	81167.5
42	85124.0
43	90185.5
44	95451.5
45	101209.5
46	105624.5
47	108695.5
48	112409.0
49	114525.5
50	114286.5
51	113182.5
52	111986.0
53	110734.5
54	108283.5
55	102800.0
56	95504.5
57	86424.5
58	77368.5
59	66895.5
60	54632.5
61	44758.5
62	37134.5
63	28896.0
64	20682.0
65	15018.5
66	11222.5
67	8578.0
68	6598.0
69	5087.5
70	3871.0
71	2815.5
72	2046.5
73	1555.5
74	1221.5
75	961.0
76	709.0
77	480.0
78	326.0
79	225.0
80	162.0
81	122.0
82	92.5
83	81.5
84	70.0
85	53.5
86	46.5
87	43.5
88	34.5
89	27.5
90	26.0
91	24.5
92	22.0
93	17.5
94	11.5
95	7.5
96	13.0
97	13.5
98	6.5
99	4.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05278032349540091
2	0.060236005555590416
3	0.05207904646993755
4	0.05768926267364449
5	0.05041812719910325
6	0.01911902627315921
7	0.016350827488435388
8	0.022145590277790592
9	0.02233013686343885
10-11	0.02388032818288419
12-13	0.029638181655109744
14-15	0.04691174207178641
16-17	0.03140982887733299
18-19	0.024470877256958606
20-21	0.028844631336822246
22-23	0.05019667129632535
24-25	0.044291180555581185
26-27	0.03211110590279636
28-29	0.02085376417825281
30-31	0.01289980633681302
32-33	0.010002424942135418
34-35	0.009374966550931351
36-37	0.0016609192708342945
38-39	0.007234226157411594
40-41	0.003912387615743005
42-43	0.21739587789364434
44-45	0.1913932639758052
46-47	0.19698502552094732
48-49	0.1949365584202517
50-51	0.19338636710080637
52-53	0.19759402925358655
54-55	0.19630220315404878
56-57	0.20672908524317518
58-59	0.20362870260428448
60-61	0.20837154985544468
62-63	0.20503125665521124
64-65	0.20610162685197114
66-67	0.19641293110543773
68-69	0.19543483420150198
70-71	0.1802466502026506
72-73	0.18796069748274766
74-75	0.1873516937501084
76-77	0.18519249869802384
78-79	0.2108075647860016
80-81	0.21892761455452486
82-83	0.21769115243068154
84-85	0.21298521449665103
86-87	0.23018495627906838
88-89	0.22955749788786434
90-91	0.2285794009839286
92-93	0.23225187803832884
94-95	0.2435830383971317
96-97	0.24454268064250262
98-99	0.23214115008693992
100-101	0.2433615824943538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2709343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.758811655922486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39551090107433	46.06427059706044
2	11.956334414623033	14.05080003928118
3	3.8800556535962802	6.83962378092483
4	1.8009280131916519	4.232815597320118
5	0.9835459240035298	2.889599485173683
6	0.6501133613320215	2.2919933132104156
7	0.44286141676987784	1.8215407404359367
8	0.29861796022413667	1.4037149185508637
9	0.23069251655066705	1.2199696317388282
>10	1.2779799103850171	13.252543969618348
>50	0.05947247464925942	2.3623007975753096
>100	0.021804219867097026	2.279975558850179
>500	0.0010100527191303432	0.4012026029990723
>1k	0.001073181014011797	0.8896489672609093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.690931712965099E-5
5	0.0	0.0	0.0	0.0	3.690931712965099E-5
6	0.0	0.0	0.0	0.0	3.690931712965099E-5
7	0.0	0.0	0.0	0.0	7.381863425930198E-5
8	0.0	0.0	0.0	0.0	7.381863425930198E-5
9	0.0	0.0	0.0	0.0	7.381863425930198E-5
10-11	0.0	0.0	0.0	0.0	7.381863425930198E-5
12-13	0.0	1.8454658564825496E-5	0.0	0.0	1.1072795138895297E-4
14-15	0.0	3.690931712965099E-5	0.0	0.0	2.0300124421308044E-4
16-17	0.0	3.690931712965099E-5	0.0	3.690931712965099E-5	2.9527453703720794E-4
18-19	0.0	3.690931712965099E-5	0.0	7.381863425930198E-5	3.690931712965099E-4
20-21	0.0	3.690931712965099E-5	0.0	9.227329282412747E-5	4.060024884261609E-4
22-23	0.0	3.690931712965099E-5	0.0	2.768198784723824E-4	4.060024884261609E-4
24-25	0.0	3.690931712965099E-5	0.0	5.536397569447649E-4	4.060024884261609E-4
26-27	0.0	3.690931712965099E-5	0.0	0.0014394633680563885	4.429118055558119E-4
28-29	0.0	3.690931712965099E-5	0.0	0.0049827578125028835	4.429118055558119E-4
30-31	0.0	7.381863425930198E-5	0.0	0.020226305787048742	4.429118055558119E-4
32-33	0.0	7.381863425930198E-5	0.0	0.05337087256947533	4.798211226854629E-4
34-35	0.0	7.381863425930198E-5	0.0	0.0964255910012132	4.798211226854629E-4
36-37	0.0	9.227329282412747E-5	0.0	0.15992807112277774	5.167304398151139E-4
38-39	0.0	1.1072795138895297E-4	0.0	0.26264670069459645	6.090037326392413E-4
40-41	0.0	1.1072795138895297E-4	0.0	0.3723411912039192	6.274583912040668E-4
42-43	0.0	1.1072795138895297E-4	0.0	0.4921857439238959	6.274583912040668E-4
44-45	0.0	1.1072795138895297E-4	0.0	0.6441598572052338	6.459130497688923E-4
46-47	1.8454658564825496E-5	1.1072795138895297E-4	0.0	0.8196267508395947	6.643677083337178E-4
48-49	3.690931712965099E-5	1.1072795138895297E-4	0.0	0.9967176544276601	7.197316840281943E-4
50-51	3.690931712965099E-5	1.1072795138895297E-4	0.0	1.1739931046013738	7.935503182874963E-4
52-53	3.690931712965099E-5	1.1072795138895297E-4	0.0	1.363153354890835	8.489142939819728E-4
54-55	5.5363975694476485E-5	1.1072795138895297E-4	0.0	1.5955344155391178	8.858236111116238E-4
56-57	7.381863425930198E-5	1.1072795138895297E-4	0.0	1.8660981647580244	8.858236111116238E-4
58-59	7.381863425930198E-5	1.1072795138895297E-4	0.0	2.142272130180638	9.042782696764493E-4
60-61	7.381863425930198E-5	1.1072795138895297E-4	0.0	2.4183907316275572	9.596422453709258E-4
62-63	7.381863425930198E-5	1.1072795138895297E-4	0.0	2.711413800319856	9.596422453709258E-4
64-65	7.381863425930198E-5	1.1072795138895297E-4	0.0	3.0386887153084716	9.780969039357513E-4
66-67	7.381863425930198E-5	1.1072795138895297E-4	0.0	3.434190503011247	9.965515625005768E-4
68-69	7.381863425930198E-5	1.1072795138895297E-4	0.0	3.848479133133014	9.965515625005768E-4
70-71	7.381863425930198E-5	1.4763726851860397E-4	0.0	4.264908503648302	0.0011072795138895297
72-73	7.381863425930198E-5	1.4763726851860397E-4	0.0	4.701988637097628	0.0011995528067136572
74-75	7.381863425930198E-5	1.4763726851860397E-4	0.0	5.171475150986789	0.0012180074652784827
76-77	7.381863425930198E-5	1.4763726851860397E-4	0.0	5.7135069276942785	0.0012180074652784827
78-79	7.381863425930198E-5	1.4763726851860397E-4	0.0	6.307008747139067	0.0012180074652784827
80-81	7.381863425930198E-5	1.4763726851860397E-4	0.0	6.907283426277146	0.001347190075232261
82-83	7.381863425930198E-5	1.4763726851860397E-4	0.0	7.5126146818619866	0.0014763726851860395
84-85	7.381863425930198E-5	1.4763726851860397E-4	0.0	8.159247463314907	0.0014763726851860395
86-87	7.381863425930198E-5	1.8454658564825494E-4	0.0	8.879034511318796	0.0014763726851860395
88-89	7.381863425930198E-5	1.8454658564825494E-4	0.0	9.647006672835444	0.0014763726851860395
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2720	0.0	60.88753	1
GTATCAA	5005	0.0	41.62289	1
TCAACGC	6205	0.0	33.578304	4
CAACGCA	6425	0.0	32.27782	5
ATCAACG	6440	0.0	32.276924	3
TATCAAC	6490	0.0	31.810062	2
AACGCAG	6830	0.0	30.284845	6
GTACATG	8395	0.0	28.489292	1
TACATGG	8730	0.0	26.909828	2
ACGCAGA	7995	0.0	25.460712	7
CGCAGAG	8135	0.0	24.850775	8
ACATGGG	9120	0.0	24.769428	3
CATGGGG	7255	0.0	23.092625	4
CCGTACG	130	2.9615837E-4	21.902605	9
GCAGAGT	9165	0.0	20.9188	9
GAGTACA	6675	0.0	19.976843	1
GTGTAAG	1245	0.0	19.8201	1
GTATAGG	795	0.0	19.697842	1
CAGAGTA	8255	0.0	19.517067	10-11
ATGGGGG	4590	0.0	19.437529	5
>>END_MODULE