FastQCFastQC Report
Mon 15 Aug 2016
SRR2921415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709343
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.055.9962461
GTATCAA49000.041.8103331
TCAACGC64250.032.0592274
ATCAACG65600.031.399473
CAACGCA66350.030.813035
TATCAAC67400.030.349662
AACGCAG69850.029.5391546
GTACATG87250.027.5668741
TACATGG90750.026.044692
ACATGGG93500.024.5172623
ACGCAGA84100.024.2513547
CGCAGAG85750.023.5629448
CATGGGG76050.021.7178334
CTAGCGA1754.0815994E-621.6964364
GTGGTAT14900.020.098181
GCAGAGT94000.019.977639
ATGGGGG47650.019.4571195
TAAGGTG14450.019.4129285
GAGTACA67500.019.365621
AGAGTAC81600.018.70206610-11
Produced by FastQC (version 0.11.5)