##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2709343
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.384106405132165	33.0	31.0	34.0	29.0	34.0
2	31.51503261122715	34.0	31.0	34.0	28.0	34.0
3	31.587602234194783	34.0	31.0	34.0	28.0	34.0
4	35.08265693933917	37.0	35.0	37.0	32.0	37.0
5	34.820616658725015	37.0	35.0	37.0	32.0	37.0
6	34.77442944654848	37.0	35.0	37.0	32.0	37.0
7	34.640736148948285	37.0	35.0	37.0	32.0	37.0
8	34.60431698755012	37.0	35.0	37.0	32.0	37.0
9	36.21640818456726	39.0	37.0	39.0	32.0	39.0
10-11	36.07048074016468	39.0	37.0	39.0	32.0	39.0
12-13	36.0025138935897	39.0	37.0	39.0	31.0	39.0
14-15	37.22886231089973	40.0	38.0	41.0	31.0	41.0
16-17	37.206221397586056	40.0	38.0	41.0	31.0	41.0
18-19	37.173927221470294	40.0	38.0	41.0	31.0	41.0
20-21	37.135150661987055	40.0	38.0	41.0	31.0	41.0
22-23	37.02508283373497	40.0	37.5	41.0	31.0	41.0
24-25	36.95293859064725	40.0	37.0	41.0	30.5	41.0
26-27	36.81002442289515	40.0	37.0	41.0	30.0	41.0
28-29	36.656052777370746	40.0	37.0	41.0	30.0	41.0
30-31	36.50261059600058	40.0	37.0	41.0	30.0	41.0
32-33	36.434983684236364	40.0	36.5	41.0	30.0	41.0
34-35	36.553993901842624	40.0	37.0	41.0	30.0	41.0
36-37	36.51993546036807	40.0	37.0	41.0	30.0	41.0
38-39	36.426231377865406	40.0	37.0	41.0	30.0	41.0
40-41	36.285860446610116	40.0	36.0	41.0	29.0	41.0
42-43	36.17245011059877	40.0	36.0	41.0	29.0	41.0
44-45	36.01940470438774	40.0	36.0	41.0	28.0	41.0
46-47	35.843085205527686	40.0	35.5	41.0	27.5	41.0
48-49	35.66910225098853	40.0	35.0	41.0	27.0	41.0
50-51	35.50700631112413	39.5	35.0	41.0	26.5	41.0
52-53	35.32808285994058	39.0	35.0	41.0	26.0	41.0
54-55	35.11751649754203	39.0	35.0	41.0	26.0	41.0
56-57	34.853068253078334	39.0	35.0	41.0	25.0	41.0
58-59	34.62935885194307	38.5	35.0	40.5	25.0	41.0
60-61	34.35617989305894	38.0	34.0	40.0	23.5	41.0
62-63	34.05276629795489	37.0	34.0	40.0	23.0	41.0
64-65	33.734963974661014	37.0	34.0	40.0	22.5	41.0
66-67	33.40044671346522	36.0	34.0	39.0	22.0	41.0
68-69	33.03733358973005	36.0	33.5	39.0	21.5	41.0
70-71	32.65761791696363	35.0	33.0	38.5	20.5	40.0
72-73	32.258777127886724	35.0	33.0	37.5	19.5	40.0
74-75	31.854779369020463	35.0	33.0	37.0	17.5	39.0
76-77	29.09330656915717	33.0	28.5	34.5	9.0	36.5
78-79	30.754925640644245	34.5	31.5	36.0	11.5	37.5
80-81	30.955988776614845	35.0	32.0	36.0	11.5	37.0
82-83	30.791836249599996	35.0	32.0	35.0	8.5	37.0
84-85	30.561308590311377	35.0	32.0	35.0	6.5	36.5
86-87	30.31799056081124	35.0	32.0	35.0	2.0	36.0
88-89	30.03918754472948	35.0	31.0	35.0	2.0	36.0
90-91	29.85025151854158	34.0	31.0	35.0	2.0	35.0
92-93	29.69921600919485	34.0	31.0	35.0	2.0	35.0
94-95	29.60007998249022	34.0	31.0	35.0	2.0	35.0
96-97	29.40764329211916	34.0	31.0	35.0	2.0	35.0
98-99	29.142511487102226	34.0	31.0	35.0	2.0	35.0
100-101	28.052308991515655	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51035.0
3	17603.0
4	9596.0
5	6692.0
6	6170.0
7	6206.0
8	6171.0
9	6819.0
10	7374.0
11	8337.0
12	8927.0
13	9821.0
14	10649.0
15	11974.0
16	13121.0
17	14640.0
18	13119.0
19	11412.0
20	10876.0
21	10612.0
22	10858.0
23	12517.0
24	14213.0
25	17250.0
26	20329.0
27	24442.0
28	29182.0
29	35386.0
30	42655.0
31	52723.0
32	67305.0
33	88894.0
34	129304.0
35	191516.0
36	328122.0
37	634933.0
38	677456.0
39	91098.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06084577850066	19.729336093449653	13.513677362117404	25.69614076593228
2	15.261175657709703	21.210367449321343	37.44850195214603	26.07995494082292
3	17.910992074272688	24.778676131867094	29.586084501718616	27.7242472921416
4	13.27828183821106	16.79014004394621	35.32642778126996	34.60515033657276
5	13.995558500966384	37.663764124280746	34.14693128598565	14.193746088767217
6	33.395169130625106	35.47886079416062	16.858473288190496	14.267496787023784
7	28.63011974507435	32.4238125978329	20.901260418131507	18.044807238961237
8	26.989654116023015	34.692373278608045	19.87723720974868	18.440735395620255
9	27.00245040956424	15.454706177844594	19.445156999316808	38.09768641327436
10-11	25.686035043045933	25.49767964100894	27.70749344515442	21.108791870790707
12-13	26.09689237510593	22.96697419589154	27.612835764925432	23.323297664077103
14-15	23.306518499762106	24.664171708443497	24.910942245986543	27.118367545807853
16-17	23.336920920282488	27.746700121284064	25.88982860044985	23.0265503579836
18-19	23.402276348585392	26.97792708848911	27.3480358124251	22.2717607505004
20-21	23.76409526927496	26.05886840699789	27.457793086189913	22.71924323753724
22-23	23.745088739487734	25.780920410359	27.114310338145707	23.359680512007557
24-25	23.504365148058483	26.12002136344393	27.018481888870966	23.35713159962662
26-27	23.141290662871285	26.41687265780053	27.133821461883116	23.308015217445067
28-29	23.311134537841305	26.74733385600534	26.852274287637922	23.089257318515436
30-31	23.798470430695488	26.231421417846327	26.697822850883142	23.272285300575042
32-33	23.446426541096145	26.303344215915146	26.609389143111116	23.64084009987759
34-35	23.82749378879523	26.398464682095764	26.710515314511856	23.06352621459715
36-37	23.74257585440767	26.31464333189324	26.820711555322863	23.12206925837623
38-39	23.610144546406637	26.449104255191536	26.73293748010353	23.207813718298294
40-41	23.783371376431784	26.226520813462827	26.742317311922893	23.247790498182493
42-43	23.574310100633895	26.247403683917366	26.4432047667016	23.73508144874714
44-45	23.40042430556645	26.28441023095031	26.678927664113882	23.636237799369354
46-47	23.625671674626915	26.28300604594832	26.39260016381903	23.698722115605737
48-49	23.330786905318465	26.508574030457837	26.456876435364997	23.7037626288587
50-51	23.541397173642984	26.43229409379566	26.51927624501394	23.507032487547416
52-53	23.888875047636976	26.192280826082825	26.406432675689317	23.512411450590882
54-55	23.11392075679576	26.169450424322733	26.769214017921488	23.947414800960022
56-57	23.26550966306435	26.27985126137705	26.492055535582328	23.962583539976272
58-59	23.325141813571097	26.187934934171004	26.631384379144013	23.855538873113883
60-61	23.40651755443138	26.28641139547717	26.730001531848607	23.57706951824284
62-63	23.42151859738861	26.170680929977408	26.774107867825652	23.633692604808328
64-65	23.097250034198964	25.971125922148946	26.71288803361227	24.21873601003982
66-67	23.128390546668967	26.18939707922606	26.4924405289922	24.189771845112766
68-69	23.156080074915867	26.103132625486936	26.575344033543054	24.165443266054147
70-71	23.094622559701385	25.992841501493864	26.47667801655645	24.4358579222483
72-73	23.056931501812166	26.226343764271455	26.30343039184595	24.413294342070433
74-75	22.959766927021082	26.296023579017007	26.325096786090786	24.419112707871125
76-77	22.949983286386612	25.8350655863648	26.52854642620029	24.686404701048307
78-79	22.872907403891823	25.855228507838536	26.56510270276659	24.706761385503054
80-81	23.047714852737087	26.06681320207188	26.274706615290643	24.610765329900385
82-83	22.98336075930091	26.039761038759675	26.409240966735364	24.56763723520405
84-85	22.83150171046027	26.082530008050636	26.304720929421155	24.78124735206794
86-87	22.65583371580496	26.259409032856233	26.250118897114977	24.834638354223827
88-89	22.56172498000754	25.996301626522005	26.288500515677747	25.153472877792698
90-91	22.614489437369368	25.729812888427293	26.426718799464606	25.228978874738733
92-93	22.709212338953986	25.587743986664503	26.496786049870373	25.206257624511142
94-95	22.535371129716843	25.48031689047804	26.597269822357216	25.387042157447897
96-97	22.37845396099844	25.264931873606404	26.639807267910413	25.71680689748474
98-99	22.09493012581119	25.335142165978052	26.541893878045165	26.028033830165597
100-101	22.079364915321552	25.372918103072816	26.54489633515274	26.002820646452896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	121.0
2	126.0
3	156.0
4	143.5
5	163.0
6	274.0
7	563.5
8	781.0
9	818.5
10	723.0
11	592.5
12	586.0
13	605.0
14	663.0
15	750.0
16	840.5
17	916.0
18	1019.0
19	1124.0
20	1301.0
21	1529.0
22	1788.5
23	2188.5
24	2644.5
25	3364.5
26	4577.0
27	6370.5
28	8714.0
29	11938.5
30	16303.5
31	21931.5
32	30426.0
33	36018.5
34	39429.0
35	45466.0
36	53296.0
37	60634.0
38	66756.5
39	72997.5
40	77107.0
41	80841.0
42	85491.0
43	90905.0
44	96185.0
45	102028.0
46	106318.0
47	109617.5
48	113563.0
49	115292.0
50	115039.5
51	114345.5
52	113216.0
53	111630.5
54	109135.5
55	103951.5
56	96211.5
57	86516.5
58	77145.0
59	66642.0
60	54488.0
61	44533.5
62	36555.0
63	27811.5
64	19691.0
65	14330.0
66	10639.5
67	8099.5
68	6198.0
69	4636.5
70	3476.0
71	2499.0
72	1763.5
73	1381.0
74	1099.5
75	844.0
76	592.0
77	345.0
78	182.0
79	108.5
80	75.5
81	42.0
82	21.0
83	13.0
84	5.5
85	4.0
86	4.5
87	3.5
88	3.5
89	2.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15291530086814403
2	6.274583912040668E-4
3	0.007049679571763339
4	0.004502936689817421
5	0.029453635069461487
6	6.274583912040668E-4
7	1.4763726851860397E-4
8	0.0019561938078715025
9	0.0
10-11	0.1017774419850126
12-13	2.5836521990755694E-4
14-15	0.005388760300929044
16-17	3.690931712965099E-5
18-19	1.4763726851860397E-4
20-21	4.429118055558119E-4
22-23	2.9527453703720794E-4
24-25	0.0015501913194453415
26-27	0.003100382638890683
28-29	0.0069020423032447355
30-31	0.01515127468172173
32-33	0.01576027841436097
34-35	0.012087801359960699
36-37	9.042782696764493E-4
38-39	2.0300124421308044E-4
40-41	0.0049827578125028835
42-43	0.007972412500004614
44-45	0.007769411255791533
46-47	0.009448785185190654
48-49	0.01179252682292349
50-51	0.00614540130208689
52-53	0.001863920515047375
54-55	9.411875868061003E-4
56-57	0.0059424000578738095
58-59	0.008876690769681063
60-61	0.007308044791670896
62-63	0.017771836197926952
64-65	0.03423339163775129
66-67	0.03637413203127105
68-69	0.02600261391783912
70-71	0.040360338281273354
72-73	0.025910340625014995
74-75	0.02448933191552343
76-77	0.2383050060475916
78-79	0.06721186649309445
80-81	0.0540721495949387
82-83	0.019764939322928107
84-85	0.031797376707194326
86-87	0.08011167282990747
88-89	0.2610965093751511
90-91	0.3155008428242566
92-93	0.05102713093174249
94-95	0.00524112303241044
96-97	0.010980521846071168
98-99	0.0016609192708342945
100-101	0.0012918260995377845
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2709343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.14816207643685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99610695362449	45.3533026847046
2	12.116980410973193	14.091602816285592
3	3.941568846631365	6.875849523880646
4	1.8600576010126058	4.326357234207572
5	1.0180337240535022	2.9598394992770816
6	0.6416620547947972	2.238688149630442
7	0.4447800785274879	1.8104200866210673
8	0.33505365254857694	1.5586203274157449
9	0.2399566327789871	1.2557733456733724
>10	1.3168876697262004	13.452611142344159
>50	0.06424962419623155	2.492420247045052
>100	0.022492617656335444	2.3026917297400527
>500	0.0012765491036209225	0.5085427705796045
>1k	8.935843724683402E-4	0.7732804425948469
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.690931712965099E-5
2	0.0	0.0	0.0	0.0	3.690931712965099E-5
3	0.0	0.0	0.0	0.0	3.690931712965099E-5
4	0.0	0.0	0.0	0.0	7.381863425930198E-5
5	0.0	0.0	0.0	0.0	7.381863425930198E-5
6	0.0	0.0	0.0	0.0	1.4763726851860397E-4
7	0.0	0.0	0.0	0.0	1.4763726851860397E-4
8	0.0	3.690931712965099E-5	0.0	0.0	1.4763726851860397E-4
9	0.0	3.690931712965099E-5	0.0	0.0	1.8454658564825494E-4
10-11	0.0	3.690931712965099E-5	0.0	0.0	1.8454658564825494E-4
12-13	0.0	3.690931712965099E-5	0.0	0.0	2.2145590277790594E-4
14-15	1.8454658564825496E-5	5.5363975694476485E-5	0.0	0.0	3.137291956020334E-4
16-17	3.690931712965099E-5	7.381863425930198E-5	0.0	1.8454658564825496E-5	3.690931712965099E-4
18-19	3.690931712965099E-5	7.381863425930198E-5	0.0	7.381863425930198E-5	4.429118055558119E-4
20-21	3.690931712965099E-5	7.381863425930198E-5	0.0	1.2918260995377847E-4	4.429118055558119E-4
22-23	3.690931712965099E-5	7.381863425930198E-5	0.0	3.137291956020334E-4	5.167304398151139E-4
24-25	3.690931712965099E-5	7.381863425930198E-5	0.0	6.274583912040668E-4	5.905490740744158E-4
26-27	3.690931712965099E-5	7.381863425930198E-5	0.0	0.0013656447337970865	6.274583912040668E-4
28-29	3.690931712965099E-5	7.381863425930198E-5	0.0	0.004853575202549105	6.274583912040668E-4
30-31	3.690931712965099E-5	7.381863425930198E-5	0.0	0.020134032494224616	6.459130497688923E-4
32-33	3.690931712965099E-5	7.381863425930198E-5	0.0	0.05386914835072562	7.381863425930198E-4
34-35	3.690931712965099E-5	7.381863425930198E-5	0.0	0.09764359846649169	7.750956597226708E-4
36-37	3.690931712965099E-5	7.381863425930198E-5	0.0	0.16205035685773267	7.750956597226708E-4
38-39	3.690931712965099E-5	7.381863425930198E-5	0.0	0.26552562743070923	7.750956597226708E-4
40-41	3.690931712965099E-5	7.381863425930198E-5	0.0	0.3759213949654953	7.750956597226708E-4
42-43	3.690931712965099E-5	1.4763726851860397E-4	0.0	0.4970762284435747	7.935503182874963E-4
44-45	3.690931712965099E-5	1.4763726851860397E-4	0.0	0.650268349190191	8.120049768523218E-4
46-47	3.690931712965099E-5	1.4763726851860397E-4	0.0	0.8262519732643671	8.120049768523218E-4
48-49	3.690931712965099E-5	1.4763726851860397E-4	0.0	1.0044686110248868	8.489142939819728E-4
50-51	3.690931712965099E-5	1.4763726851860397E-4	0.0	1.1817256065400357	8.858236111116238E-4
52-53	3.690931712965099E-5	1.4763726851860397E-4	0.0	1.3712549500007936	9.780969039357513E-4
54-55	3.690931712965099E-5	1.4763726851860397E-4	0.0	1.6047986541386603	0.0010703701967598787
56-57	3.690931712965099E-5	1.4763726851860397E-4	0.0	1.8757314965288634	0.0010703701967598787
58-59	5.5363975694476485E-5	1.6609192708342944E-4	0.0	2.151056547657495	0.0011072795138895297
60-61	7.381863425930198E-5	1.8454658564825494E-4	0.0	2.4283562472525624	0.0011441888310191807
62-63	7.381863425930198E-5	1.8454658564825494E-4	0.0	2.7220436836531956	0.0011810981481488317
64-65	7.381863425930198E-5	1.8454658564825494E-4	0.0	3.049632327837413	0.0011810981481488317
66-67	7.381863425930198E-5	1.8454658564825494E-4	0.0	3.445244843491577	0.0011995528067136572
68-69	7.381863425930198E-5	1.8454658564825494E-4	0.0	3.8599948400774653	0.0012180074652784827
70-71	7.381863425930198E-5	1.8454658564825494E-4	0.0	4.279100136084653	0.0012180074652784827
72-73	7.381863425930198E-5	1.8454658564825494E-4	0.0	4.7188931043430085	0.0013287354166674355
74-75	7.381863425930198E-5	1.8454658564825494E-4	0.0	5.192255096530783	0.0013287354166674355
76-77	7.381863425930198E-5	2.2145590277790594E-4	0.0	5.7395464509292475	0.0013287354166674355
78-79	7.381863425930198E-5	2.2145590277790594E-4	0.0	6.3384554853335295	0.0013287354166674355
80-81	7.381863425930198E-5	2.2145590277790594E-4	0.0	6.946794850264437	0.001347190075232261
82-83	7.381863425930198E-5	2.2145590277790594E-4	0.0	7.559950881080764	0.001421008709491563
84-85	7.381863425930198E-5	2.2145590277790594E-4	0.0	8.213577978129752	0.0014394633680563885
86-87	7.381863425930198E-5	2.2145590277790594E-4	0.0	8.942333990196147	0.0014394633680563885
88-89	7.381863425930198E-5	2.2145590277790594E-4	0.0	9.719865664849376	0.001457918026621214
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2640	0.0	55.996246	1
GTATCAA	4900	0.0	41.810333	1
TCAACGC	6425	0.0	32.059227	4
ATCAACG	6560	0.0	31.39947	3
CAACGCA	6635	0.0	30.81303	5
TATCAAC	6740	0.0	30.34966	2
AACGCAG	6985	0.0	29.539154	6
GTACATG	8725	0.0	27.566874	1
TACATGG	9075	0.0	26.04469	2
ACATGGG	9350	0.0	24.517262	3
ACGCAGA	8410	0.0	24.251354	7
CGCAGAG	8575	0.0	23.562944	8
CATGGGG	7605	0.0	21.717833	4
CTAGCGA	175	4.0815994E-6	21.696436	4
GTGGTAT	1490	0.0	20.09818	1
GCAGAGT	9400	0.0	19.97763	9
ATGGGGG	4765	0.0	19.457119	5
TAAGGTG	1445	0.0	19.412928	5
GAGTACA	6750	0.0	19.36562	1
AGAGTAC	8160	0.0	18.702066	10-11
>>END_MODULE