FastQCFastQC Report
Mon 15 Aug 2016
SRR2921414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047291
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.045.7899441
GTATCAA41600.040.9512441
TCAACGC46900.035.5175554
CAACGCA48100.034.333775
ATCAACG50650.032.885223
AACGCAG51800.031.8719436
TATCAAC52700.031.5175152
GTACATG82400.029.1975751
TACATGG83450.027.9790692
ACATGGG86550.026.8659463
ACGCAGA61350.026.2202537
CGCAGAG65000.024.7507298
CATGGGG78550.024.0461564
ATGGGGG46500.022.6562165
GCAGAGT78950.018.994579
GTATATA13750.018.2910731
GTGTAGC21300.017.6000331
AGAGTAC65300.017.29692610-11
TAGCCCT20800.017.1122344
CAGAGTA71350.017.06076810-11
Produced by FastQC (version 0.11.5)