##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921414_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3047291
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.310267381749888	31.0	31.0	34.0	26.0	34.0
2	30.36785886218284	31.0	31.0	34.0	26.0	34.0
3	30.419830925238188	33.0	31.0	34.0	26.0	34.0
4	33.87054764379247	37.0	35.0	37.0	30.0	37.0
5	33.86216347569037	37.0	35.0	37.0	30.0	37.0
6	33.9065845697047	37.0	35.0	37.0	30.0	37.0
7	33.827471022623044	37.0	35.0	37.0	29.0	37.0
8	33.79285503091106	37.0	35.0	37.0	29.0	37.0
9	35.34463462793675	39.0	37.0	39.0	29.0	39.0
10-11	35.23501907103719	39.0	35.0	39.0	29.0	39.0
12-13	35.12431090434094	39.0	35.0	39.0	28.0	39.0
14-15	36.25609254252383	40.0	37.0	41.0	27.0	41.0
16-17	36.20680466683359	40.0	36.5	41.0	27.0	41.0
18-19	36.157310706460265	40.0	36.5	41.0	27.0	41.0
20-21	36.09956417027452	40.0	36.5	41.0	27.0	41.0
22-23	35.98979995674847	40.0	36.0	41.0	27.0	41.0
24-25	35.89772752257661	40.0	36.0	41.0	27.0	41.0
26-27	35.756120107990995	40.0	36.0	41.0	27.0	41.0
28-29	35.620065001996856	40.0	36.0	41.0	26.0	41.0
30-31	35.486394144832246	40.0	36.0	41.0	25.0	41.0
32-33	35.3152168598273	39.5	35.0	41.0	25.0	41.0
34-35	35.16172758033282	39.0	35.0	41.0	24.5	41.0
36-37	35.00165048234645	39.0	35.0	41.0	24.0	41.0
38-39	35.232228887887636	39.5	35.0	41.0	24.5	41.0
40-41	35.361394267892365	40.0	35.0	41.0	24.5	41.0
42-43	35.21356821517866	40.0	35.0	41.0	24.0	41.0
44-45	35.10854936400888	40.0	35.0	41.0	23.5	41.0
46-47	34.96951111659503	40.0	35.0	41.0	23.0	41.0
48-49	34.84138764561704	40.0	35.0	41.0	22.0	41.0
50-51	33.16478390150465	37.0	32.5	39.5	18.0	40.0
52-53	33.32872377465756	37.5	33.5	39.5	18.0	40.0
54-55	34.19332367666888	39.0	34.0	40.5	18.5	41.0
56-57	34.22316821727889	39.0	34.0	41.0	19.0	41.0
58-59	34.06205659387305	39.0	34.0	41.0	18.0	41.0
60-61	33.833302103409224	38.0	34.0	40.5	16.0	41.0
62-63	33.559651014622496	37.5	34.0	40.0	13.5	41.0
64-65	33.283313277268235	37.0	33.5	40.0	12.5	41.0
66-67	32.95917734801173	36.5	33.0	40.0	8.0	41.0
68-69	32.60369291938315	36.0	33.0	39.0	7.5	41.0
70-71	32.23099155938832	35.5	33.0	39.0	4.5	41.0
72-73	31.872581909637116	35.0	32.0	38.5	2.0	40.0
74-75	31.47388139170168	35.0	32.0	37.0	2.0	39.5
76-77	31.093287447769185	35.0	32.0	37.0	2.0	39.0
78-79	30.743753222124177	35.0	31.5	36.0	2.0	39.0
80-81	30.38871180993217	35.0	31.0	36.0	2.0	37.5
82-83	30.084912796316466	35.0	31.0	35.5	2.0	37.0
84-85	29.841489375317288	35.0	31.0	35.0	2.0	37.0
86-87	29.61176910902175	35.0	31.0	35.0	2.0	36.0
88-89	29.416665982999326	35.0	31.0	35.0	2.0	36.0
90-91	29.171390261054817	35.0	31.0	35.0	2.0	36.0
92-93	28.985367331180385	34.0	30.0	35.0	2.0	35.0
94-95	28.791881215151427	34.0	30.0	35.0	2.0	35.0
96-97	28.556010568075052	34.0	30.0	35.0	2.0	35.0
98-99	28.34960248299227	34.0	30.0	35.0	2.0	35.0
100-101	27.327858415884798	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111854.0
3	18474.0
4	12408.0
5	9152.0
6	8209.0
7	8572.0
8	9008.0
9	9517.0
10	10357.0
11	11438.0
12	11402.0
13	11351.0
14	11964.0
15	12869.0
16	12958.0
17	13679.0
18	12818.0
19	12075.0
20	12122.0
21	12922.0
22	13920.0
23	16076.0
24	18834.0
25	22232.0
26	26357.0
27	31400.0
28	36869.0
29	43960.0
30	52118.0
31	63975.0
32	79881.0
33	103254.0
34	145025.0
35	209605.0
36	343594.0
37	635300.0
38	727192.0
39	144528.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01659886182422	18.022812764405234	13.259291386590697	24.701296987179845
2	15.679602453341493	19.053324616587407	40.44789013485235	24.81918279521875
3	19.512215141726113	23.374261147184832	29.08992769970408	28.02359601138497
4	13.204325012969786	15.603636793475047	36.899104920638045	34.29293327291712
5	14.268979236578765	37.21094928112223	34.38767426108637	14.132397221212631
6	33.67532549373826	35.12778837557061	17.239778964905625	13.957107165785512
7	29.187951041670768	31.587587910868848	20.701114205031054	18.523346842429326
8	26.40343224236027	35.72268841916893	19.522962688708013	18.350916649762787
9	26.602108792807368	16.078265070870774	19.348521928189186	37.97110420813268
10-11	25.820583041029437	25.385659792239263	27.68694060108064	21.106816565650664
12-13	26.068842003919656	22.61773128926407	27.709954522320235	23.603472184496034
14-15	23.23032684665347	24.32832437349973	24.898367761211475	27.542981018635327
16-17	23.626328445604365	27.443641539989965	25.638001024497843	23.292028989907827
18-19	23.54702173263703	26.936308701260764	26.859271246024218	22.65739832007799
20-21	24.05223405372758	26.12825293453428	26.91845999317245	22.90105301856569
22-23	23.852864166523027	25.919972421265175	26.515377607052276	23.711785805159522
24-25	23.64375461572466	26.03010170640027	26.625592770258834	23.700550907616236
26-27	23.577895098621397	26.24489265339766	26.551046220276287	23.626166027704656
28-29	23.557298514132555	26.585767451463017	26.368957073317556	23.48797696108688
30-31	24.04160612030395	26.183548401124746	26.37753197299229	23.397313505579017
32-33	23.733737841892793	26.152518740218827	26.35941147811635	23.754331939772026
34-35	23.938208225611522	26.331802596481957	26.392534314405196	23.33745486350132
36-37	23.926797520318754	26.26246350598811	26.444299796554038	23.3664391771391
38-39	23.82308554543132	26.36352701931696	26.36223069989598	23.45115673535573
40-41	23.980192762528105	26.125938535079612	26.409824988374453	23.48404371401783
42-43	23.715112598353986	26.153074564035943	26.287156634628822	23.844656202981245
44-45	23.68864353307117	26.254418698011513	26.433081805444292	23.623855963473027
46-47	23.88644518518214	26.12834308120394	26.2035749955816	23.781636738032315
48-49	23.634929470766888	26.26873214410776	26.31122953754616	23.785108847579195
50-51	23.782037255534085	26.321936226419375	26.336633402933945	23.55939311511259
52-53	23.92481921942776	26.18469145642458	26.291308590924277	23.599180733223378
54-55	23.505686391590952	26.036235867866857	26.669222594578102	23.78885514596409
56-57	23.648477338892107	26.203181299057306	26.38539355512521	23.76294780692538
58-59	23.742792687700863	26.211590527163647	26.494038085946332	23.551578699189157
60-61	23.904269338877697	26.196185209940058	26.513062215393163	23.38648323578908
62-63	23.81852067119203	26.1432436454037	26.57684994200113	23.461385741403138
64-65	23.57224539189355	25.956679976553552	26.585426208227798	23.885648423325105
66-67	23.550851991278694	26.152941172602258	26.419752558702942	23.876454277416105
68-69	23.591348829333292	26.077471231676352	26.508303542291223	23.822876396699133
70-71	23.557685116315387	25.950367171158877	26.516505237377082	23.975442475148657
72-73	23.485231060374424	26.153542921357477	26.428547474902896	23.932678543365206
74-75	23.476014057711975	26.156605830902734	26.500158058024965	23.867222053360322
76-77	23.480582561777226	25.857281668786964	26.54003957411714	24.12209619531867
78-79	23.43207179097272	25.873956040920433	26.622715918164396	24.07125624994245
80-81	23.56091506285976	26.020184320218064	26.405144021958982	24.013756594963194
82-83	23.58675467242117	26.078981212638347	26.51104563866522	23.823218476275265
84-85	23.617815794276805	26.067249082062695	26.439258283633997	23.875676840026504
86-87	23.32888466772744	26.20038730526809	26.498258770901945	23.972469256102524
88-89	23.283762993198845	25.991709679249613	26.61000545177808	24.114521875773466
90-91	23.457978875963384	25.889607963351324	26.630827331787888	24.021585828897408
92-93	23.492029398689922	25.82182409828816	26.677095793094114	24.009050709927806
94-95	23.414446556576642	25.805867436354905	26.668493513897335	24.111192493171117
96-97	23.28966980795352	25.61296872167817	26.791255583769786	24.306105886598527
98-99	23.073328898277975	25.57840056773094	26.79650579037867	24.551764743612413
100-101	23.285800426927697	25.545378913956206	26.86637768396495	24.302442975151152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	17.0
2	33.0
3	56.0
4	59.5
5	76.5
6	124.0
7	243.5
8	370.5
9	434.0
10	423.5
11	408.0
12	448.0
13	503.5
14	554.5
15	658.5
16	754.0
17	844.0
18	974.5
19	1075.0
20	1162.0
21	1262.0
22	1428.0
23	1671.5
24	1973.5
25	2411.5
26	3196.5
27	4578.0
28	6765.5
29	10322.5
30	15280.5
31	21701.5
32	29727.5
33	37081.5
34	43761.0
35	50939.5
36	59612.5
37	68058.5
38	75428.5
39	82962.0
40	89367.0
41	95209.0
42	100834.5
43	107010.5
44	113483.0
45	120058.0
46	125414.5
47	128764.5
48	132332.5
49	134601.5
50	134141.0
51	132087.0
52	129266.0
53	125649.5
54	121333.5
55	114386.5
56	104847.5
57	94294.5
58	83687.5
59	72419.0
60	59788.0
61	48713.0
62	39354.5
63	29932.0
64	21461.5
65	15703.5
66	11776.5
67	8669.0
68	6393.0
69	4874.0
70	3647.0
71	2660.5
72	1923.5
73	1434.5
74	1115.5
75	822.5
76	596.0
77	415.5
78	271.0
79	197.0
80	150.5
81	116.5
82	94.5
83	93.5
84	87.0
85	64.0
86	53.0
87	46.5
88	40.5
89	35.5
90	26.5
91	26.5
92	28.5
93	22.0
94	15.0
95	20.0
96	16.5
97	7.5
98	7.5
99	7.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05053668980087559
2	0.0594626505968744
3	0.049946001218787436
4	0.05746087262424232
5	0.04928968057202282
6	0.017556577300953533
7	0.014931294713895062
8	0.02142886911686478
9	0.02031312401736493
10-11	0.021707805391739745
12-13	0.027942851536003618
14-15	0.045368164707604235
16-17	0.03020715776734155
18-19	0.023660359315864483
20-21	0.026613802226305265
22-23	0.04810830340784651
24-25	0.04323512260561922
26-27	0.03097833452728998
28-29	0.020329532033534044
30-31	0.012584948401711553
32-33	0.009894033749976619
34-35	0.009303345167888461
36-37	0.0014603134390512753
38-39	0.006645246548491759
40-41	0.0040035559452641705
42-43	0.2171108699497357
44-45	0.19074318796596715
46-47	0.197831450951025
48-49	0.19453343970103282
50-51	0.19330283848834917
52-53	0.19804475516122352
54-55	0.1967321138676943
56-57	0.20779111676567813
58-59	0.2033117283515096
60-61	0.20882482178433237
62-63	0.2061995391972739
64-65	0.207348100329112
66-67	0.1970274581587384
68-69	0.19586248901073117
70-71	0.17896223235654227
72-73	0.18721546448960733
74-75	0.18660836789135007
76-77	0.18562388692120313
78-79	0.21209001700198635
80-81	0.22029402508654408
82-83	0.21843991925943404
84-85	0.2135011063925303
86-87	0.2314678840977117
88-89	0.22966300231910902
90-91	0.22849803317110182
92-93	0.23258362919721157
94-95	0.24433176877429824
96-97	0.24461070504917318
98-99	0.23360092619969672
100-101	0.24388875233773208
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3047291.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.60103568009708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.45799843508384	51.976700276540456
2	11.359605491032031	14.67684559275974
3	3.494320688319124	6.7721020639141525
4	1.5457970257834792	3.9944035526730595
5	0.8500267470843604	2.7456304108716814
6	0.514444520045521	1.994018927693467
7	0.35215152530264465	1.592454727561373
8	0.24304715174311345	1.2560878177362274
9	0.19307752644001247	1.122570735711852
>10	0.9365951731736948	10.417457206339902
>50	0.04065639962081847	1.7621201822890922
>100	0.01131109724195752	1.2287617316487165
>500	8.663013264419886E-4	0.3858221440999221
>1k	1.0191780297126841E-4	0.07502463016036685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.281603233823091E-5
2	0.0	0.0	0.0	0.0	3.281603233823091E-5
3	0.0	0.0	0.0	0.0	3.281603233823091E-5
4	0.0	0.0	0.0	0.0	3.281603233823091E-5
5	0.0	3.281603233823091E-5	0.0	0.0	3.281603233823091E-5
6	0.0	3.281603233823091E-5	0.0	0.0	1.3126412935292363E-4
7	0.0	3.281603233823091E-5	0.0	0.0	1.3126412935292363E-4
8	0.0	3.281603233823091E-5	0.0	0.0	1.3126412935292363E-4
9	0.0	3.281603233823091E-5	0.0	0.0	1.6408016169115454E-4
10-11	0.0	3.281603233823091E-5	0.0	0.0	1.6408016169115454E-4
12-13	0.0	3.281603233823091E-5	0.0	1.6408016169115454E-5	1.6408016169115454E-4
14-15	0.0	3.281603233823091E-5	0.0	4.922404850734636E-5	1.6408016169115454E-4
16-17	0.0	3.281603233823091E-5	0.0	6.563206467646182E-5	2.133042101985009E-4
18-19	0.0	3.281603233823091E-5	0.0	6.563206467646182E-5	2.6252825870584726E-4
20-21	0.0	3.281603233823091E-5	0.0	6.563206467646182E-5	2.789362748749627E-4
22-23	0.0	3.281603233823091E-5	0.0	6.563206467646182E-5	3.117523072131936E-4
24-25	0.0	4.922404850734636E-5	0.0	1.6408016169115454E-4	3.281603233823091E-4
26-27	0.0	6.563206467646182E-5	0.0	2.789362748749627E-4	3.281603233823091E-4
28-29	0.0	6.563206467646182E-5	0.0	0.0010008889863160426	3.4456833955142453E-4
30-31	0.0	9.844809701469272E-5	0.0	0.0045942445273523275	3.937923880587709E-4
32-33	0.0	9.844809701469272E-5	0.0	0.012798252611910053	4.266084203970018E-4
34-35	0.0	9.844809701469272E-5	0.0	0.02425104789795264	5.414645335808099E-4
36-37	0.0	1.1485611318380818E-4	0.0	0.043366386734972145	6.070965982572717E-4
38-39	0.0	1.3126412935292363E-4	0.0	0.07862721348240126	6.399126305955028E-4
40-41	0.0	1.3126412935292363E-4	0.0	0.12204282426588076	6.563206467646182E-4
42-43	0.0	1.3126412935292363E-4	0.0	0.16567173925955875	6.563206467646182E-4
44-45	0.0	1.3126412935292363E-4	0.0	0.21706164590122834	6.891366791028491E-4
46-47	0.0	1.3126412935292363E-4	0.0	0.2798879398127714	7.875847761175418E-4
48-49	0.0	1.3126412935292363E-4	0.0	0.3511971780837472	8.532168407940036E-4
50-51	0.0	1.3126412935292363E-4	0.0	0.43016895990569987	8.532168407940036E-4
52-53	0.0	1.3126412935292363E-4	0.0	0.5122090407512772	9.844809701469272E-4
54-55	0.0	1.3126412935292363E-4	0.0	0.6069489261117498	0.0010665210509925045
56-57	1.6408016169115454E-5	1.3126412935292363E-4	0.0	0.715143384730897	0.0010829290671616199
58-59	3.281603233823091E-5	1.3126412935292363E-4	0.0	0.8372846570937925	0.0010829290671616199
60-61	3.281603233823091E-5	1.3126412935292363E-4	0.0	0.9716991255511863	0.0010829290671616199
62-63	3.281603233823091E-5	1.3126412935292363E-4	0.0	1.1218816975471	0.0011157450994998509
64-65	3.281603233823091E-5	1.3126412935292363E-4	0.0	1.2758052972295721	0.0011157450994998509
66-67	3.281603233823091E-5	1.3126412935292363E-4	0.0	1.455292586103526	0.0011321531156689663
68-69	3.281603233823091E-5	1.3126412935292363E-4	0.0	1.6541741500893745	0.0011485611318380817
70-71	3.281603233823091E-5	1.3126412935292363E-4	0.0	1.8730406777692055	0.001197785180345428
72-73	3.281603233823091E-5	1.3126412935292363E-4	0.0	2.1027036800883145	0.0013782733582056981
74-75	3.281603233823091E-5	1.3126412935292363E-4	0.0	2.3405870985081503	0.00144390542288216
76-77	3.281603233823091E-5	1.6408016169115454E-4	0.0	2.6078244578545338	0.00144390542288216
78-79	3.281603233823091E-5	1.6408016169115454E-4	0.0	2.9122095658077947	0.0014603134390512753
80-81	3.281603233823091E-5	1.6408016169115454E-4	0.0	3.233872314787134	0.0015095374875586215
82-83	3.281603233823091E-5	1.6408016169115454E-4	0.0	3.574355058312449	0.0015751695522350836
84-85	3.281603233823091E-5	1.6408016169115454E-4	0.0	3.9285877193874823	0.001591577568404199
86-87	3.281603233823091E-5	1.9689619402938545E-4	0.0	4.30703861232813	0.0016243936007424298
88-89	3.281603233823091E-5	1.9689619402938545E-4	0.0	4.726755665934103	0.0016408016169115454
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	45.789944	1
GTATCAA	4160	0.0	40.951244	1
TCAACGC	4690	0.0	35.517555	4
CAACGCA	4810	0.0	34.33377	5
ATCAACG	5065	0.0	32.88522	3
AACGCAG	5180	0.0	31.871943	6
TATCAAC	5270	0.0	31.517515	2
GTACATG	8240	0.0	29.197575	1
TACATGG	8345	0.0	27.979069	2
ACATGGG	8655	0.0	26.865946	3
ACGCAGA	6135	0.0	26.220253	7
CGCAGAG	6500	0.0	24.750729	8
CATGGGG	7855	0.0	24.046156	4
ATGGGGG	4650	0.0	22.656216	5
GCAGAGT	7895	0.0	18.99457	9
GTATATA	1375	0.0	18.291073	1
GTGTAGC	2130	0.0	17.600033	1
AGAGTAC	6530	0.0	17.296926	10-11
TAGCCCT	2080	0.0	17.112234	4
CAGAGTA	7135	0.0	17.060768	10-11
>>END_MODULE