FastQCFastQC Report
Mon 15 Aug 2016
SRR2921414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047291
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.049.518251
GTATCAA40700.038.6504941
TCAACGC48050.032.0978974
CAACGCA49700.031.3758875
ATCAACG51250.030.1863293
AACGCAG52400.029.9372026
TATCAAC52950.028.9483052
GTATAGG9500.026.5139081
GTACATG80650.026.1637551
TACATGG81750.024.9032552
GTATAGC9450.023.6367361
ACGCAGA65750.023.2076247
ACATGGG87300.022.6133923
CGCAGAG67700.022.117878
CATGGGG74150.021.055784
ATGGGGG47900.018.1632735
GCAGAGT80200.017.7222259
AGAGTAC67150.017.66224710-11
TATAGGG7100.017.3780732
GAGTACA60750.016.0372181
Produced by FastQC (version 0.11.5)