##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3047291
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.487707934686906	33.0	31.0	34.0	30.0	34.0
2	31.618398439794557	34.0	31.0	34.0	30.0	34.0
3	31.69147580588792	34.0	31.0	34.0	29.0	34.0
4	35.182633690054544	37.0	35.0	37.0	33.0	37.0
5	34.94074638752912	37.0	35.0	37.0	32.0	37.0
6	34.90542058503766	37.0	35.0	37.0	32.0	37.0
7	34.77537524312578	37.0	35.0	37.0	32.0	37.0
8	34.743956189284184	37.0	35.0	37.0	32.0	37.0
9	36.379756314707066	39.0	37.0	39.0	32.0	39.0
10-11	36.228045336004996	39.0	37.0	39.0	32.0	39.0
12-13	36.16354591668469	39.0	37.0	39.0	32.0	39.0
14-15	37.42706735260926	40.0	38.0	41.0	32.0	41.0
16-17	37.409080721204504	40.0	38.0	41.0	32.0	41.0
18-19	37.375306460722	40.0	38.0	41.0	32.0	41.0
20-21	37.34272210957208	40.0	38.0	41.0	32.0	41.0
22-23	37.23910401074266	40.0	38.0	41.0	31.0	41.0
24-25	37.18136502224435	40.0	38.0	41.0	31.0	41.0
26-27	37.0535073611283	40.0	37.5	41.0	31.0	41.0
28-29	36.90678097365824	40.0	37.0	41.0	30.0	41.0
30-31	36.77871099281296	40.0	37.0	41.0	30.0	41.0
32-33	36.73876699009054	40.0	37.0	41.0	30.0	41.0
34-35	36.87304740505583	40.0	37.0	41.0	30.0	41.0
36-37	36.85839849230021	40.0	37.0	41.0	30.0	41.0
38-39	36.78291866447937	40.0	37.0	41.0	30.0	41.0
40-41	36.6737948558244	40.0	37.0	41.0	30.0	41.0
42-43	36.58675115044805	40.0	37.0	41.0	30.0	41.0
44-45	36.467375449210465	40.0	37.0	41.0	30.0	41.0
46-47	36.32889507434636	40.0	36.5	41.0	29.5	41.0
48-49	36.18705515817163	40.0	36.0	41.0	29.0	41.0
50-51	36.049823433338005	40.0	36.0	41.0	28.0	41.0
52-53	35.8932911231648	40.0	35.5	41.0	28.0	41.0
54-55	35.698728149034665	39.0	35.0	41.0	28.0	41.0
56-57	35.45120961536	39.0	35.0	41.0	27.0	41.0
58-59	35.239889790636994	39.0	35.0	41.0	27.0	41.0
60-61	34.98161875580639	38.5	35.0	40.0	26.5	41.0
62-63	34.697300651627955	38.0	34.5	40.0	26.0	41.0
64-65	34.39659832290385	37.0	34.0	40.0	26.0	41.0
66-67	34.070962865049644	37.0	34.0	40.0	26.0	41.0
68-69	33.71397414949868	36.0	34.0	39.0	25.5	41.0
70-71	33.33433909003111	36.0	34.0	39.0	25.0	40.5
72-73	32.934808818718	35.0	33.5	38.5	24.0	40.0
74-75	32.523293147914	35.0	33.0	37.0	22.5	39.5
76-77	29.753617721445046	33.0	29.0	34.5	18.5	36.5
78-79	31.40270784772442	34.5	31.5	36.0	20.5	38.5
80-81	31.588798706785795	35.0	33.0	36.0	21.0	37.0
82-83	31.411956390118306	35.0	33.0	35.5	20.5	37.0
84-85	31.16883011829195	35.0	32.5	35.0	20.0	37.0
86-87	30.910851966549963	35.0	32.0	35.0	18.5	36.0
88-89	30.629010652412255	35.0	32.0	35.0	17.5	36.0
90-91	30.4418562257428	35.0	32.0	35.0	12.5	35.5
92-93	30.293321838971075	34.5	32.0	35.0	8.5	35.0
94-95	30.195895633203392	34.0	32.0	35.0	4.5	35.0
96-97	30.01155173562354	34.0	32.0	35.0	2.0	35.0
98-99	29.757967322451318	34.0	31.5	35.0	2.0	35.0
100-101	28.682931003307527	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52163.0
3	17201.0
4	9506.0
5	6640.0
6	5689.0
7	6081.0
8	5959.0
9	6440.0
10	7289.0
11	7940.0
12	8409.0
13	9288.0
14	10198.0
15	11218.0
16	12016.0
17	12829.0
18	12232.0
19	10676.0
20	10260.0
21	10176.0
22	10997.0
23	12404.0
24	14573.0
25	17350.0
26	21408.0
27	25704.0
28	30829.0
29	37229.0
30	45550.0
31	56948.0
32	72059.0
33	95810.0
34	138870.0
35	205098.0
36	359668.0
37	721295.0
38	829287.0
39	119996.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52860468005471	19.687479253037026	13.917769391713836	25.866146675194422
2	14.610373841589878	20.508289359691265	38.92691186894542	25.954424929773438
3	17.882541897516244	24.61384316384418	29.07739808432866	28.426216854310915
4	12.808245027353248	16.83043610957052	35.88227765434813	34.4790412087281
5	13.924489407377743	37.51068453221116	34.54127085207092	14.023555208340172
6	33.46384432142654	35.928884145819204	16.888784751380996	13.718486781373256
7	28.820896786053936	31.822949106777777	20.82048855340595	18.535665553762335
8	26.231298589210528	36.37926904106352	19.210955523541053	18.178476846184896
9	26.175937906816248	16.10292551646692	19.316960539705594	38.40417603701123
10-11	25.65685131846588	25.748617673802553	27.665396249323294	20.929134758408274
12-13	26.003104081231825	22.798277216867444	27.741097507169105	23.457521194731626
14-15	23.02923216803619	24.588203460628456	24.944771357604782	27.43779301373057
16-17	23.487283590004605	27.751951492526295	25.607549413690815	23.153215503778284
18-19	23.394703373943873	27.19102399369667	26.890132657670556	22.524139974688897
20-21	23.90008962117252	26.250422098986277	26.954511101900685	22.894977177940522
22-23	23.62284697548603	26.08430198311086	26.64841105995078	23.64443998145233
24-25	23.47560130183272	26.273212584820538	26.66194108279863	23.589245030548113
26-27	23.365163178893358	26.44611392289788	26.653878939384366	23.534843958824396
28-29	23.413619342154988	26.76961312703565	26.40029300129583	23.416474529513533
30-31	23.853383847318494	26.37501874909947	26.429994226754317	23.34160317682772
32-33	23.601944452923227	26.377804931763304	26.35816150669829	23.662089108615174
34-35	23.789991998482403	26.492653897933714	26.477638744936694	23.239715358647185
36-37	23.751526570573052	26.449842096445952	26.533621963123405	23.26500936985759
38-39	23.725624002083165	26.518929716299965	26.39334253387154	23.36210374774533
40-41	23.938937625742643	26.28670790853016	26.34584520304488	23.428509262682315
42-43	23.643444752814094	26.318296661813307	26.289990395560793	23.74826818981181
44-45	23.602486408555436	26.420166682775164	26.394108653776254	23.58323825489314
46-47	23.748903021523425	26.316111619077876	26.222675636345972	23.712309723052726
48-49	23.510399393488367	26.486080672939096	26.313858282059087	23.68966165151345
50-51	23.70486509370339	26.4471311387985	26.294265231210506	23.553738536287604
52-53	23.90123449497545	26.3268573048774	26.210407588773833	23.561500611373315
54-55	23.414547063139835	26.190574639316267	26.59903887679062	23.79583942075328
56-57	23.54433138115794	26.305616774695135	26.32629207937282	23.823759764774106
58-59	23.6359790568562	26.357908396473068	26.42348084344283	23.5826317032279
60-61	23.776064502916913	26.361396049170082	26.452762929201167	23.40977651871183
62-63	23.695158415744093	26.29734174016076	26.465472818398744	23.542027025696402
64-65	23.45788518832144	26.151933818186475	26.44608578942816	23.944095204063927
66-67	23.475174580913887	26.336916214292653	26.308519732436203	23.879389472357257
68-69	23.460886186204768	26.2547405796904	26.43001093244517	23.85436230165966
70-71	23.466886978035397	26.1044513577186	26.369570241812845	24.059091422433152
72-73	23.443942949990973	26.314546439298198	26.243266753106077	23.998243857604752
74-75	23.375863938825777	26.36485594017027	26.260063968236462	23.999216152767495
76-77	23.35258259723773	26.093616590125222	26.391393804279907	24.162407008357135
78-79	23.302115611203572	26.08887117049546	26.45052561891414	24.15848759938683
80-81	23.485633392067925	26.260633502882747	26.13213756207066	24.121595542978667
82-83	23.48953768893703	26.337912132998536	26.256741010618217	23.915809167446213
84-85	23.492608155509483	26.343775138106224	26.159272414881478	24.004344291502814
86-87	23.259715441660546	26.44732980847218	26.171314032482435	24.12164071738484
88-89	23.207346518142877	26.262390783751005	26.26686571328732	24.2633969848188
90-91	23.340172991498576	26.15241655575885	26.28723436652502	24.220176086217553
92-93	23.35390495552665	26.117554894186085	26.359354769124543	24.169185381162723
94-95	23.30873431849724	26.07972522439887	26.299226061412977	24.312314395690912
96-97	23.04256124618906	25.9903763900419	26.436866863987373	24.53019549978167
98-99	22.935250386742652	25.915203432870204	26.359358581486365	24.79018759890078
100-101	22.976758182679465	26.02574227306014	26.33260967408424	24.664889870176154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	21.0
2	27.0
3	40.5
4	38.0
5	39.0
6	75.0
7	186.5
8	295.5
9	347.5
10	314.5
11	247.0
12	271.0
13	306.5
14	324.0
15	369.0
16	435.5
17	493.0
18	554.5
19	624.0
20	696.0
21	847.5
22	1082.0
23	1369.5
24	1790.5
25	2385.5
26	3409.5
27	5065.0
28	7516.0
29	11442.5
30	16778.0
31	23205.0
32	31320.5
33	38548.5
34	44889.5
35	51882.0
36	60334.0
37	68954.5
38	76118.5
39	83424.5
40	89806.5
41	95438.5
42	101157.5
43	107304.0
44	113674.0
45	120412.5
46	125510.0
47	129311.0
48	133293.5
49	135232.5
50	134544.0
51	132112.0
52	129591.5
53	126344.5
54	121581.0
55	114305.5
56	104533.5
57	93682.5
58	83156.5
59	71601.0
60	58584.0
61	47535.5
62	38552.0
63	29092.5
64	20483.5
65	14656.5
66	10638.5
67	7959.5
68	5897.0
69	4374.0
70	3254.0
71	2356.5
72	1622.0
73	1137.5
74	841.5
75	591.5
76	415.0
77	262.0
78	142.0
79	78.5
80	51.5
81	32.5
82	14.5
83	13.5
84	8.5
85	3.5
86	4.5
87	4.0
88	3.0
89	2.0
90	2.0
91	1.5
92	0.5
93	0.5
94	0.5
95	1.5
96	1.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15348058324590597
2	3.6097635572054E-4
3	0.006989814888043183
4	0.00397073991292594
5	0.027270122873069885
6	0.0012470092288527745
7	2.9534429104407817E-4
8	0.0020017779726320853
9	0.0
10-11	0.10327205376841266
12-13	4.1020040422788635E-4
14-15	0.006087373998741833
16-17	2.6252825870584726E-4
18-19	3.7738437188965544E-4
20-21	6.563206467646182E-4
22-23	2.6252825870584726E-4
24-25	0.0015915775684041992
26-27	0.003232379185315744
28-29	0.006743694645506452
30-31	0.015686063457674374
32-33	0.015423535198968528
34-35	0.012109115932807205
36-37	6.399126305955026E-4
38-39	1.8048817786027E-4
40-41	0.004741916672874366
42-43	0.008614208488785613
44-45	0.008056335939035687
46-47	0.009352569216395809
48-49	0.01176454759325578
50-51	0.00577562169152864
52-53	0.001739249713926238
54-55	7.547687437793109E-4
56-57	0.005824845740035986
58-59	0.009172081038535538
60-61	0.007711767599484264
62-63	0.01840979414174754
64-65	0.03532645881210557
66-67	0.037377460833245
68-69	0.028041299633018307
70-71	0.04262802600736195
72-73	0.027598283196452193
74-75	0.025563689191481876
76-77	0.24397079241857766
78-79	0.06953717252471128
80-81	0.054786365988676505
82-83	0.021035076728806012
84-85	0.032635544160370636
86-87	0.08327068205826092
88-89	0.2670240551361849
90-91	0.3235168548064494
92-93	0.05219389943395626
94-95	0.004922404850734636
96-97	0.010025297879329542
98-99	0.001476721455220391
100-101	0.0015259455037277374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3047291.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.87873270168352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84499352726947	51.00396999096096
2	11.774353829611064	15.042616020335378
3	3.5968089375635506	6.8927879010494495
4	1.5959130767794556	4.077796193868647
5	0.8390882262610911	2.679994625923099
6	0.517589730255234	1.9837785616866344
7	0.3655605637535129	1.6346081876801168
8	0.2483851540989485	1.269322309260256
9	0.19450627981930424	1.1182333191638574
>10	0.9682030813052696	10.768347583927133
>50	0.04181518436691293	1.7828376493237164
>100	0.01185463463825001	1.301944169254812
>500	8.246882472435542E-4	0.36662015396525593
>1k	1.0308603072844664E-4	0.0771433336005384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.563206467646182E-5
2	0.0	0.0	0.0	3.281603233823091E-5	6.563206467646182E-5
3	0.0	0.0	0.0	3.281603233823091E-5	6.563206467646182E-5
4	0.0	0.0	0.0	3.281603233823091E-5	6.563206467646182E-5
5	0.0	0.0	0.0	3.281603233823091E-5	6.563206467646182E-5
6	0.0	0.0	0.0	3.281603233823091E-5	1.6408016169115454E-4
7	0.0	0.0	0.0	3.281603233823091E-5	1.6408016169115454E-4
8	0.0	0.0	0.0	3.281603233823091E-5	1.6408016169115454E-4
9	0.0	0.0	0.0	3.281603233823091E-5	1.6408016169115454E-4
10-11	0.0	0.0	0.0	6.563206467646182E-5	1.6408016169115454E-4
12-13	0.0	0.0	0.0	8.204008084557727E-5	1.6408016169115454E-4
14-15	0.0	0.0	0.0	1.3126412935292363E-4	2.133042101985009E-4
16-17	0.0	0.0	0.0	1.6408016169115454E-4	2.6252825870584726E-4
18-19	0.0	0.0	0.0	1.6408016169115454E-4	2.6252825870584726E-4
20-21	0.0	0.0	0.0	1.6408016169115454E-4	2.9534429104407817E-4
22-23	0.0	0.0	0.0	1.9689619402938545E-4	3.117523072131936E-4
24-25	0.0	0.0	0.0	2.9534429104407817E-4	3.6097635572054E-4
26-27	0.0	0.0	0.0	4.1020040422788635E-4	3.6097635572054E-4
28-29	0.0	0.0	0.0	0.0011649691480071973	3.6097635572054E-4
30-31	0.0	3.281603233823091E-5	0.0	0.004725508656705251	3.7738437188965544E-4
32-33	0.0	3.281603233823091E-5	0.0	0.013126412935292363	4.266084203970018E-4
34-35	0.0	4.922404850734636E-5	0.0	0.024841736480040795	4.594244527352327E-4
36-37	0.0	6.563206467646182E-5	0.0	0.04423601159193526	4.922404850734636E-4
38-39	0.0	6.563206467646182E-5	0.0	0.07944761429085703	4.922404850734636E-4
40-41	0.0	6.563206467646182E-5	0.0	0.12274836896115271	5.250565174116945E-4
42-43	0.0	6.563206467646182E-5	0.0	0.1662624278416469	5.414645335808099E-4
44-45	0.0	6.563206467646182E-5	0.0	0.21852195934027963	5.742805659190409E-4
46-47	0.0	6.563206467646182E-5	0.0	0.2820537979470946	6.563206467646182E-4
48-49	0.0	6.563206467646182E-5	0.0	0.35449518933373936	7.2195271144108E-4
50-51	0.0	6.563206467646182E-5	0.0	0.4338607635437508	7.2195271144108E-4
52-53	0.0	6.563206467646182E-5	0.0	0.5155890920821149	8.368088246248882E-4
54-55	0.0	6.563206467646182E-5	0.0	0.6111986022995506	9.352569216395808E-4
56-57	0.0	6.563206467646182E-5	0.0	0.720246277759492	9.680729539778118E-4
58-59	0.0	6.563206467646182E-5	0.0	0.8429126066397991	0.001017297002485158
60-61	0.0	9.844809701469272E-5	0.0	0.9786069003583839	0.001050113034823389
62-63	0.0	9.844809701469272E-5	0.0	1.1296426891950917	0.0010829290671616199
64-65	0.0	9.844809701469272E-5	0.0	1.2838944492009459	0.0010829290671616199
66-67	0.0	9.844809701469272E-5	0.0	1.4636442663336058	0.0010829290671616199
68-69	0.0	9.844809701469272E-5	0.0	1.6635923513704467	0.0010829290671616199
70-71	0.0	9.844809701469272E-5	0.0	1.8820979026945572	0.0010829290671616199
72-73	0.0	9.844809701469272E-5	0.0	2.1129094661455046	0.0011649691480071973
74-75	0.0	9.844809701469272E-5	0.0	2.3521711579235456	0.0011813771641763127
76-77	0.0	9.844809701469272E-5	0.0	2.622624488439076	0.001197785180345428
78-79	0.0	9.844809701469272E-5	0.0	2.92935594270452	0.0012141931965145435
80-81	0.0	9.844809701469272E-5	0.0	3.255301183903999	0.0012470092288527743
82-83	0.0	9.844809701469272E-5	0.0	3.6005914761668643	0.0013126412935292363
84-85	0.0	9.844809701469272E-5	0.0	3.958712837073978	0.0013126412935292363
86-87	0.0	9.844809701469272E-5	0.0	4.341561078347949	0.0013126412935292363
88-89	0.0	9.844809701469272E-5	0.0	4.76669277728973	0.0013126412935292363
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2265	0.0	49.51825	1
GTATCAA	4070	0.0	38.650494	1
TCAACGC	4805	0.0	32.097897	4
CAACGCA	4970	0.0	31.375887	5
ATCAACG	5125	0.0	30.186329	3
AACGCAG	5240	0.0	29.937202	6
TATCAAC	5295	0.0	28.948305	2
GTATAGG	950	0.0	26.513908	1
GTACATG	8065	0.0	26.163755	1
TACATGG	8175	0.0	24.903255	2
GTATAGC	945	0.0	23.636736	1
ACGCAGA	6575	0.0	23.207624	7
ACATGGG	8730	0.0	22.613392	3
CGCAGAG	6770	0.0	22.11787	8
CATGGGG	7415	0.0	21.05578	4
ATGGGGG	4790	0.0	18.163273	5
GCAGAGT	8020	0.0	17.722225	9
AGAGTAC	6715	0.0	17.662247	10-11
TATAGGG	710	0.0	17.378073	2
GAGTACA	6075	0.0	16.037218	1
>>END_MODULE