FastQCFastQC Report
Mon 15 Aug 2016
SRR2921413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153833
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.046.5062981
GTATCAA46100.043.6430741
TCAACGC56700.034.4814264
CAACGCA58250.033.7246865
ATCAACG59700.032.826623
TATCAAC60050.032.4787942
AACGCAG61400.032.1393856
GTACATG101700.028.6015721
TACATGG104650.027.1618082
ACGCAGA73400.026.2441277
ACATGGG109450.024.9288643
CGCAGAG76850.024.699058
CATGGGG92550.023.0731644
ATGGGGG54050.021.5086865
GAGTACA76450.020.6068211
GCAGAGT88250.020.2717299
GTATATA9100.019.8149321
AGTACAT78050.018.6045672
GTATAGC7850.018.134351
AGAGTAC71750.017.98945810-11
Produced by FastQC (version 0.11.5)