##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921413_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3153833
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.552639597594418	31.0	31.0	34.0	27.0	34.0
2	30.60508213339134	33.0	31.0	34.0	27.0	34.0
3	30.659273335018057	33.0	31.0	34.0	27.0	34.0
4	34.118232005309096	37.0	35.0	37.0	30.0	37.0
5	34.11148148934963	37.0	35.0	37.0	30.0	37.0
6	34.16196006573588	37.0	35.0	37.0	30.0	37.0
7	34.08096877672344	37.0	35.0	37.0	30.0	37.0
8	34.04524209113165	37.0	35.0	37.0	30.0	37.0
9	35.61932004643239	39.0	37.0	39.0	30.0	39.0
10-11	35.513215347800596	39.0	37.0	39.0	30.0	39.0
12-13	35.40238021480529	39.0	37.0	39.0	30.0	39.0
14-15	36.5615465371819	40.0	37.0	41.0	29.5	41.0
16-17	36.51039164090172	40.0	37.0	41.0	29.0	41.0
18-19	36.45084505108545	40.0	37.0	41.0	29.0	41.0
20-21	36.38644944104523	40.0	37.0	41.0	28.5	41.0
22-23	36.272151854584564	40.0	37.0	41.0	28.0	41.0
24-25	36.176789798318424	40.0	36.5	41.0	27.0	41.0
26-27	36.036893995338374	40.0	36.0	41.0	27.0	41.0
28-29	35.89685741128335	40.0	36.0	41.0	27.0	41.0
30-31	35.756536569945204	40.0	36.0	41.0	27.0	41.0
32-33	35.58402585044928	40.0	35.5	41.0	26.0	41.0
34-35	35.42085424307501	39.0	35.0	41.0	25.0	41.0
36-37	35.257502378851385	39.0	35.0	41.0	25.0	41.0
38-39	35.498988215292314	39.5	35.0	41.0	25.5	41.0
40-41	35.62270402396069	40.0	35.5	41.0	26.0	41.0
42-43	35.463359981330655	40.0	35.0	41.0	25.0	41.0
44-45	35.34989994714368	40.0	35.0	41.0	24.5	41.0
46-47	35.19981067482013	40.0	35.0	41.0	24.0	41.0
48-49	35.055303498948746	39.5	35.0	41.0	24.0	41.0
50-51	33.32881972507739	37.0	32.5	39.5	20.5	40.0
52-53	33.48617095451789	37.5	33.5	39.5	21.0	40.0
54-55	34.345469306713454	39.0	34.0	40.5	22.0	41.0
56-57	34.34977549540511	39.0	34.0	41.0	22.0	41.0
58-59	34.16170165002396	38.0	34.0	41.0	21.5	41.0
60-61	33.906025461715956	38.0	34.0	40.0	20.0	41.0
62-63	33.60780088863298	37.0	34.0	40.0	19.5	41.0
64-65	33.3082939077624	37.0	34.0	40.0	18.0	41.0
66-67	32.95871373024507	36.0	33.0	39.0	17.0	41.0
68-69	32.58721118080761	36.0	33.0	39.0	13.5	41.0
70-71	32.207339925734814	35.0	33.0	38.5	11.0	40.5
72-73	31.85054566934901	35.0	32.5	37.5	7.5	40.0
74-75	31.45412296719579	35.0	32.0	37.0	6.0	39.0
76-77	31.092854948248686	35.0	32.0	36.5	2.0	39.0
78-79	30.762170983688737	35.0	32.0	36.0	2.0	38.0
80-81	30.428322140075267	35.0	31.5	35.5	2.0	37.0
82-83	30.14558491207366	35.0	31.0	35.0	2.0	37.0
84-85	29.925331334918496	35.0	31.0	35.0	2.0	36.0
86-87	29.715530594042235	35.0	31.0	35.0	2.0	36.0
88-89	29.53114828844774	35.0	31.0	35.0	2.0	36.0
90-91	29.29232730458461	35.0	31.0	35.0	2.0	35.0
92-93	29.114928564702062	34.5	31.0	35.0	2.0	35.0
94-95	28.916712298970808	34.0	30.0	35.0	2.0	35.0
96-97	28.674832338934877	34.0	30.0	35.0	2.0	35.0
98-99	28.462832527911274	34.0	30.0	35.0	2.0	35.0
100-101	27.421481733496986	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100207.0
3	17589.0
4	12196.0
5	9316.0
6	8623.0
7	8534.0
8	9154.0
9	9835.0
10	10611.0
11	11228.0
12	11573.0
13	11399.0
14	12401.0
15	12939.0
16	13207.0
17	13530.0
18	13057.0
19	12099.0
20	12414.0
21	13266.0
22	14291.0
23	16461.0
24	19465.0
25	23034.0
26	27536.0
27	32379.0
28	38551.0
29	45845.0
30	54757.0
31	66897.0
32	84036.0
33	109509.0
34	154591.0
35	226751.0
36	383760.0
37	699968.0
38	713237.0
39	119567.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05982082779349	17.36540908576111	12.392823594465135	25.181946491980266
2	16.317070230208888	18.96342546765146	39.072438386761085	25.647065915378565
3	19.9287614982165	22.799873613203108	28.674131439730882	28.59723344884951
4	13.6753096654189	14.770635187280837	35.58638858564526	35.96766656165501
5	14.715547714950365	36.82676284344109	33.86364220361894	14.594047237989615
6	34.87232049360572	33.97576369539585	16.634330029055405	14.517585781943026
7	30.177661908022234	31.171018494939823	20.02759605739802	18.62372353963992
8	27.571080303911096	34.47552121539066	19.195987262297905	18.757411218400343
9	27.35612994972025	15.457455668253859	18.67429415208499	38.512120229940905
10-11	26.478563018580697	24.77158068748984	27.042921534503332	21.706934759426126
12-13	26.780898450545955	21.94274381469604	27.142848987144287	24.133508747613718
14-15	23.851011158397476	23.613892039903263	24.19783950382309	28.33725729787617
16-17	24.303937425692453	26.80427085317313	25.01976380488886	23.872027916245553
18-19	24.374129623362993	26.144694932493184	26.23782582730776	23.243349616836063
20-21	24.75656378628232	25.349716554043123	26.274602899731114	23.619116759943445
22-23	24.658398156763308	25.050606211819314	25.997412667208287	24.293582964209094
24-25	24.374459743024044	25.33376580326002	25.9419464636067	24.349827990109233
26-27	24.180448757391645	25.491962279614473	26.006443624313487	24.321145338680395
28-29	24.3058163135862	25.7996638335659	25.756009767854877	24.138510084993023
30-31	24.650956297691014	25.483254385095627	25.64552175205629	24.220267565157073
32-33	24.359458351732073	25.540106341400765	25.63149620781206	24.468939099055103
34-35	24.626443023464144	25.595736213487402	25.745520503181353	24.0323002598671
36-37	24.640761876375823	25.47731025424152	25.744147720561834	24.137780148820827
38-39	24.545362444046773	25.597004856453903	25.682097044413936	24.175535655085387
40-41	24.612264498989052	25.406752673858897	25.771844342339552	24.209138484812502
42-43	24.448223036732905	25.368226043081354	25.6704180874126	24.513132832773138
44-45	24.34445725697854	25.455790588511512	25.829893991327474	24.369858163182474
46-47	24.52891577270904	25.455126041075054	25.59845581314068	24.41750237307523
48-49	24.35543518719009	25.574149001286944	25.614420649839843	24.455995161683123
50-51	24.3533085543434	25.579374501278746	25.77092794441932	24.296388999958538
52-53	24.602344200044673	25.460234102267464	25.673562724620204	24.26385897306766
54-55	24.087059563281926	25.27673972414277	26.027808134326495	24.608392578248804
56-57	24.23689222035058	25.470063715317004	25.802510798492644	24.490533265839776
58-59	24.332588989591102	25.385744029958047	25.88568564226856	24.395981338182292
60-61	24.35988184560708	25.38414398044806	25.974042069026993	24.28193210491787
62-63	24.348766392256216	25.374349894454667	26.003459179552262	24.27342453373685
64-65	24.15050140229948	25.19768196368956	25.96935977227648	24.68245686173448
66-67	24.178595440143464	25.37603755863771	25.806709386990466	24.63865761422836
68-69	24.198845244162356	25.304241814972173	25.902748740838273	24.594164200027198
70-71	24.162421906283253	25.14113772020431	25.926693045094655	24.769747328417782
72-73	24.128817410498986	25.395823127202853	25.80198740762062	24.673372054677536
74-75	24.012077616221163	25.47676180352207	25.917425144735372	24.593735435521396
76-77	24.05441605026637	25.094123952294222	25.96343819365882	24.88802180378059
78-79	23.94280709693329	25.072231557913632	26.092975012266333	24.891986332886745
80-81	24.08973782847714	25.292970177647632	25.824327218942813	24.79296477493242
82-83	24.081482378387722	25.30541511380946	25.91564151872216	24.69746098908066
84-85	24.023773038791035	25.31209899204197	25.949659935717968	24.71446803344903
86-87	23.84384913165025	25.39828267290991	25.964278159884547	24.79359003555529
88-89	23.778823608684498	25.14214545638314	26.08887599169678	24.99015494323558
90-91	23.880968219818456	25.073269323125263	26.108209437908837	24.93755301914744
92-93	23.932294261584747	25.000099327007874	26.184267968692765	24.883338442714617
94-95	23.89370839838625	24.84803326555592	26.230956376785635	25.0273019592722
96-97	23.76962909747476	24.736604531699005	26.338529613712076	25.155236757114157
98-99	23.57327662928777	24.72221692475907	26.310756604877056	25.393749841076108
100-101	23.767625305261586	24.660145412990218	26.427384497280958	25.144844784467242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	33.0
2	55.0
3	91.0
4	88.5
5	95.0
6	175.0
7	332.5
8	503.5
9	620.0
10	589.5
11	524.5
12	555.5
13	602.5
14	697.0
15	782.5
16	864.5
17	942.0
18	1018.5
19	1149.5
20	1306.0
21	1442.5
22	1587.0
23	1773.0
24	1973.0
25	2323.5
26	2819.0
27	3565.5
28	4760.5
29	6588.0
30	9519.0
31	13961.5
32	20318.5
33	25891.0
34	31008.5
35	37572.5
36	46016.0
37	54891.0
38	63052.5
39	71493.0
40	79119.5
41	85848.0
42	92836.5
43	100524.5
44	108557.0
45	117554.5
46	124489.0
47	130528.5
48	137739.5
49	141638.5
50	142458.0
51	142661.0
52	142348.0
53	141517.5
54	139750.5
55	134818.0
56	126030.5
57	114557.5
58	103241.5
59	90502.5
60	75129.5
61	62346.5
62	51693.0
63	40097.0
64	29235.0
65	21228.0
66	15660.0
67	12005.5
68	9246.0
69	7039.5
70	5459.0
71	4047.5
72	2929.0
73	2288.5
74	1833.5
75	1406.5
76	1047.0
77	740.0
78	480.5
79	325.5
80	254.0
81	187.0
82	131.0
83	106.0
84	88.5
85	78.0
86	63.0
87	51.0
88	51.0
89	45.0
90	38.5
91	32.0
92	25.5
93	27.0
94	25.5
95	19.0
96	13.0
97	11.0
98	12.5
99	9.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05295144035844637
2	0.060656350542340066
3	0.05089045615287809
4	0.057136823668215785
5	0.050161184818600095
6	0.01734397477608992
7	0.015346405469154518
8	0.021370820839277157
9	0.021402528288593594
10-11	0.02205253099958051
12-13	0.029044023573854418
14-15	0.04467579608685685
16-17	0.03086720190954943
18-19	0.023590342291427605
20-21	0.026983039368286145
22-23	0.049209961339107045
24-25	0.044327014144376065
26-27	0.03009036940129677
28-29	0.020070815417303326
30-31	0.012540296204650024
32-33	0.009845163012753053
34-35	0.009448819896297617
36-37	0.0012841516973156155
38-39	0.006167098892046598
40-41	0.003313428453567453
42-43	0.22697777593169963
44-45	0.19999473656341346
46-47	0.207541109500725
48-49	0.20475085396087872
50-51	0.20319718894437339
52-53	0.20850818670487625
54-55	0.20662159347054837
56-57	0.21770334700664237
58-59	0.21451674835034068
60-61	0.21984359983550175
62-63	0.2170057831216808
64-65	0.21748139486142734
66-67	0.207398425978801
68-69	0.20613012800614364
70-71	0.18810444306975035
72-73	0.1970617975016432
74-75	0.19633252616736524
76-77	0.1941130047152148
78-79	0.2222375122588926
80-81	0.23041803418253284
82-83	0.22873753936876176
84-85	0.22433020391377728
86-87	0.24118271322546248
88-89	0.2404692956158427
90-91	0.239835146629514
92-93	0.2428632080392335
94-95	0.2535486184588721
96-97	0.2534376423862646
98-99	0.24329125860500542
100-101	0.2525656875300626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3153833.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.979503899148895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79286812780435	44.32599032814205
2	11.953731886457996	13.622354252676278
3	3.9605703731275588	6.770140050554262
4	1.866268439865346	4.253561993846641
5	1.0690603896065491	3.045726531900601
6	0.6867548461300982	2.3478570259697595
7	0.47405936801854104	1.8908167325908367
8	0.3431428333104467	1.5641686726862079
9	0.28409731887896456	1.4568951859921537
>10	1.4689270354591921	15.014528252587994
>50	0.07675169215742059	2.9670614209584993
>100	0.0223690869867022	2.0384016370539246
>500	9.510494943168512E-4	0.39466497369217995
>1k	4.475527025463965E-4	0.30783294134869127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.1707449316434956E-5	0.0
5	0.0	0.0	0.0	3.1707449316434956E-5	0.0
6	0.0	0.0	0.0	3.1707449316434956E-5	0.0
7	0.0	0.0	0.0	3.1707449316434956E-5	0.0
8	0.0	0.0	0.0	3.1707449316434956E-5	0.0
9	0.0	0.0	0.0	3.1707449316434956E-5	0.0
10-11	0.0	0.0	0.0	3.1707449316434956E-5	0.0
12-13	0.0	1.5853724658217478E-5	0.0	3.1707449316434956E-5	4.7561173974652434E-5
14-15	0.0	4.7561173974652434E-5	0.0	3.1707449316434956E-5	7.926862329108739E-5
16-17	0.0	6.341489863286991E-5	0.0	6.341489863286991E-5	9.512234794930487E-5
18-19	0.0	6.341489863286991E-5	0.0	6.341489863286991E-5	9.512234794930487E-5
20-21	0.0	6.341489863286991E-5	0.0	7.926862329108739E-5	1.2682979726573982E-4
22-23	0.0	6.341489863286991E-5	0.0	1.9024469589860974E-4	1.2682979726573982E-4
24-25	0.0	6.341489863286991E-5	0.0	3.32928217822567E-4	1.2682979726573982E-4
26-27	0.0	6.341489863286991E-5	0.0	7.292713342780039E-4	1.5853724658217478E-4
28-29	0.0	6.341489863286991E-5	0.0	0.0032183061056181477	1.5853724658217478E-4
30-31	0.0	6.341489863286991E-5	0.0	0.012778102074523286	1.5853724658217478E-4
32-33	0.0	6.341489863286991E-5	0.0	0.03316599198499096	1.5853724658217478E-4
34-35	0.0	6.341489863286991E-5	0.0	0.05999049410669493	1.5853724658217478E-4
36-37	0.0	9.512234794930487E-5	0.0	0.10300164910443894	1.5853724658217478E-4
38-39	0.0	1.2682979726573982E-4	0.0	0.17391535950064574	1.9024469589860974E-4
40-41	0.0	1.2682979726573982E-4	0.0	0.25524496699730137	1.9024469589860974E-4
42-43	0.0	1.5853724658217478E-4	0.0	0.339412391207778	1.9024469589860974E-4
44-45	0.0	1.5853724658217478E-4	0.0	0.44146281683272387	2.219521452150447E-4
46-47	0.0	1.5853724658217478E-4	0.0	0.5577974483747237	2.5365959453147965E-4
48-49	0.0	1.5853724658217478E-4	0.0	0.682772359855452	2.5365959453147965E-4
50-51	0.0	1.5853724658217478E-4	0.0	0.8170217002612377	3.1707449316434956E-4
52-53	0.0	1.5853724658217478E-4	0.0	0.9539344664096039	3.4878194248078454E-4
54-55	0.0	1.5853724658217478E-4	0.0	1.1123448831945129	3.804893917972195E-4
56-57	0.0	1.5853724658217478E-4	0.0	1.2894468413514604	3.804893917972195E-4
58-59	0.0	1.5853724658217478E-4	0.0	1.478153725958223	3.804893917972195E-4
60-61	0.0	1.5853724658217478E-4	0.0	1.6888655803905914	3.804893917972195E-4
62-63	0.0	1.5853724658217478E-4	0.0	1.9057445337150063	3.804893917972195E-4
64-65	0.0	1.5853724658217478E-4	0.0	2.1358613471290333	3.804893917972195E-4
66-67	0.0	1.9024469589860974E-4	0.0	2.4040746608967565	3.804893917972195E-4
68-69	0.0	2.2195214521504467E-4	0.0	2.700951508846537	3.804893917972195E-4
70-71	0.0	2.3780586987326216E-4	0.0	3.0135076904832943	3.9634311645543694E-4
72-73	0.0	2.5365959453147965E-4	0.0	3.3431858947509268	4.2805056577187187E-4
74-75	0.0	2.5365959453147965E-4	0.0	3.6853568340492346	4.4390429043008933E-4
76-77	0.0	2.853670438479146E-4	0.0	4.069714534663059	4.914654644047418E-4
78-79	0.0	2.853670438479146E-4	0.0	4.495307773112907	5.707340876958292E-4
80-81	0.0	2.853670438479146E-4	0.0	4.9421608563294255	5.707340876958292E-4
82-83	0.0	2.853670438479146E-4	0.0	5.400460328749176	6.341489863286991E-4
84-85	0.0	2.853670438479146E-4	0.0	5.879464765572559	6.341489863286991E-4
86-87	0.0	2.853670438479146E-4	0.0	6.3960266761112585	6.500027109869166E-4
88-89	0.0	2.853670438479146E-4	0.0	6.959024146173878	6.65856435645134E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2510	0.0	46.506298	1
GTATCAA	4610	0.0	43.643074	1
TCAACGC	5670	0.0	34.481426	4
CAACGCA	5825	0.0	33.724686	5
ATCAACG	5970	0.0	32.82662	3
TATCAAC	6005	0.0	32.478794	2
AACGCAG	6140	0.0	32.139385	6
GTACATG	10170	0.0	28.601572	1
TACATGG	10465	0.0	27.161808	2
ACGCAGA	7340	0.0	26.244127	7
ACATGGG	10945	0.0	24.928864	3
CGCAGAG	7685	0.0	24.69905	8
CATGGGG	9255	0.0	23.073164	4
ATGGGGG	5405	0.0	21.508686	5
GAGTACA	7645	0.0	20.606821	1
GCAGAGT	8825	0.0	20.271729	9
GTATATA	910	0.0	19.814932	1
AGTACAT	7805	0.0	18.604567	2
GTATAGC	785	0.0	18.13435	1
AGAGTAC	7175	0.0	17.989458	10-11
>>END_MODULE