FastQCFastQC Report
Mon 15 Aug 2016
SRR2921413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153833
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43600.046.2210961
GGTATCA23600.045.918081
TCAACGC55550.035.285214
CAACGCA57150.034.275195
ATCAACG59800.033.253673
AACGCAG60100.032.9066166
TATCAAC60250.032.7689862
GTACATG97750.030.2436711
TACATGG101850.028.5178742
ACATGGG108350.025.843413
ACGCAGA76550.025.4622127
CGCAGAG78300.024.5288438
CATGGGG90300.022.9678544
TATAGCG1302.9599416E-421.9045662
ATGGGGG55800.019.8527455
GCAGAGT91900.019.3469949
TCTATAC8950.018.5597343
AGAGTAC75550.018.46847710-11
TACCGTA2603.255809E-718.2845427
ATAGCAC8550.017.7627663
Produced by FastQC (version 0.11.5)