FastQCFastQC Report
Mon 15 Aug 2016
SRR2921412_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921412_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196389
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22050.045.6216471
GTATCAA40150.044.2007641
TCAACGC48150.035.2834664
CAACGCA49100.034.5975
TATCAAC51950.033.1593172
AACGCAG51800.032.6913346
ATCAACG52350.032.6329773
GTACATG88600.029.5094811
TACATGG91450.028.2292732
ACGCAGA63250.026.3282687
ACATGGG96500.026.308583
CGCAGAG65250.024.9407628
CATGGGG83700.022.3385624
GCAGAGT78050.019.6347669
GAGTACA64900.019.15581
GTATAGG9100.018.2503171
GTGTAGC18100.018.0889851
AGAGTAC65100.017.9653410-11
AGTACAT65600.017.5787092
TCTATAC8200.016.7824383
Produced by FastQC (version 0.11.5)