##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921412_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3196389
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.36418940247886	31.0	31.0	34.0	26.0	34.0
2	30.412546157554665	31.0	31.0	34.0	26.0	34.0
3	30.46654740708969	33.0	31.0	34.0	26.0	34.0
4	33.92144291574023	37.0	35.0	37.0	30.0	37.0
5	33.91159524075449	37.0	35.0	37.0	30.0	37.0
6	33.95576351939642	37.0	35.0	37.0	30.0	37.0
7	33.8716435953196	37.0	35.0	37.0	29.0	37.0
8	33.83508046110783	37.0	35.0	37.0	29.0	37.0
9	35.395286681314445	39.0	37.0	39.0	30.0	39.0
10-11	35.28387862053086	39.0	35.0	39.0	29.0	39.0
12-13	35.174304504239004	39.0	35.0	39.0	28.0	39.0
14-15	36.31725597228623	40.0	37.0	41.0	28.0	41.0
16-17	36.26840021661944	40.0	37.0	41.0	27.5	41.0
18-19	36.210584506454	40.0	36.5	41.0	27.0	41.0
20-21	36.14587116899726	40.0	37.0	41.0	27.0	41.0
22-23	36.02775084634567	40.0	36.0	41.0	27.0	41.0
24-25	35.93548860917742	40.0	36.0	41.0	27.0	41.0
26-27	35.79167742098975	40.0	36.0	41.0	27.0	41.0
28-29	35.651716202251976	40.0	36.0	41.0	26.0	41.0
30-31	35.516475935813816	40.0	36.0	41.0	25.5	41.0
32-33	35.348997728374115	39.5	35.0	41.0	25.0	41.0
34-35	35.186566466096586	39.0	35.0	41.0	24.5	41.0
36-37	35.02193584698233	39.0	35.0	41.0	24.0	41.0
38-39	35.25970133797857	39.5	35.0	41.0	24.5	41.0
40-41	35.39385037303032	40.0	35.0	41.0	25.0	41.0
42-43	35.241075163254536	40.0	35.0	41.0	24.0	41.0
44-45	35.13264468123248	40.0	35.0	41.0	23.5	41.0
46-47	34.994027791986525	40.0	35.0	41.0	23.0	41.0
48-49	34.85475657061765	39.5	35.0	41.0	22.5	41.0
50-51	33.14677468856263	37.0	32.5	39.5	18.5	40.0
52-53	33.306895374749445	37.5	33.5	39.5	19.0	40.0
54-55	34.170369282337035	39.0	34.0	40.5	19.5	41.0
56-57	34.18633761410141	39.0	34.0	41.0	19.0	41.0
58-59	34.010597740137385	38.5	34.0	41.0	18.5	41.0
60-61	33.76754034005248	38.0	34.0	40.0	18.0	41.0
62-63	33.47989872321548	37.0	34.0	40.0	14.5	41.0
64-65	33.18727241897028	37.0	33.0	40.0	13.0	41.0
66-67	32.848169293537175	36.0	33.0	39.5	10.0	41.0
68-69	32.47805163889627	36.0	33.0	39.0	7.5	41.0
70-71	32.09828356310825	35.0	32.5	39.0	6.5	40.5
72-73	31.74011986651187	35.0	32.0	37.5	2.0	40.0
74-75	31.33729671200846	35.0	32.0	37.0	2.0	39.0
76-77	30.96604950148433	35.0	32.0	36.5	2.0	39.0
78-79	30.62342130447827	35.0	31.5	36.0	2.0	38.5
80-81	30.278642242855923	35.0	31.0	36.0	2.0	37.0
82-83	29.986561554303933	35.0	31.0	35.0	2.0	37.0
84-85	29.75927804782209	35.0	31.0	35.0	2.0	36.5
86-87	29.542156633626256	35.0	31.0	35.0	2.0	36.0
88-89	29.350636452571948	35.0	31.0	35.0	2.0	36.0
90-91	29.10929161000116	34.5	30.0	35.0	2.0	35.5
92-93	28.928979388929193	34.0	30.0	35.0	2.0	35.0
94-95	28.7294991316764	34.0	30.0	35.0	2.0	35.0
96-97	28.492452733381327	34.0	30.0	35.0	2.0	35.0
98-99	28.27428373079747	34.0	30.0	35.0	2.0	35.0
100-101	27.241444799115502	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114116.0
3	19268.0
4	12933.0
5	9855.0
6	8978.0
7	8958.0
8	9694.0
9	10141.0
10	11028.0
11	11888.0
12	11877.0
13	11883.0
14	12545.0
15	13274.0
16	13446.0
17	13743.0
18	13227.0
19	12666.0
20	12662.0
21	13196.0
22	14915.0
23	17190.0
24	19769.0
25	23692.0
26	28267.0
27	33683.0
28	39797.0
29	47020.0
30	56160.0
31	68463.0
32	85527.0
33	111322.0
34	154126.0
35	225198.0
36	375979.0
37	689285.0
38	734635.0
39	125972.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.500968635855834	17.69349788509027	12.776039307615521	25.029494171438373
2	16.025112461378086	19.16557883566082	39.43270004351324	25.376608659447854
3	19.48355044047458	23.045973125791928	29.017076841404112	28.453399592329383
4	13.421228180068297	15.291255373690834	36.28459423527758	35.002922210963284
5	14.584053267625244	36.8247955386611	34.26990524412658	14.321245949587071
6	34.26256572779649	34.686377808568615	16.890651226188734	14.160405237446163
7	29.703845508775178	31.334375524120166	20.48455613436501	18.477222832739642
8	26.99566789082234	34.864372814231245	19.465608485476608	18.674350809469807
9	26.89423240607226	15.62744081951454	18.989043849635504	38.4892829247777
10-11	26.003690692782527	24.963152528816128	27.603825377721787	21.42933140067956
12-13	26.436207333598293	22.293071667975827	27.44578976851316	23.824931229912718
14-15	23.564789245761293	23.974790214501652	24.57080811256669	27.88961242717037
16-17	23.962999879514115	27.11880690806614	25.302568227089296	23.615624985330452
18-19	24.02750098612324	26.59081119334759	26.420936698946	22.96075112158317
20-21	24.497152987602703	25.694196914054494	26.413014734990792	23.39563536335201
22-23	24.335942060649387	25.513672420932963	26.10438435840364	24.04600116001401
24-25	24.08893928797176	25.741722819198525	26.122058183501522	24.047279709328194
26-27	24.031358189862573	25.87556087580733	26.115332104981086	23.977748829349014
28-29	24.12840423762585	26.019599121257926	25.961364700647383	23.89063194046884
30-31	24.437856658767167	25.8028831201177	25.817385704925748	23.94187451618939
32-33	24.128786231413603	25.84114575103869	25.8624999882669	24.167568029280805
34-35	24.41078115300667	25.88110619956347	25.91057965718176	23.7975329902481
36-37	24.462272449042963	25.738918317471725	25.92118903328474	23.87762020020057
38-39	24.29383312555936	25.883245366384855	25.894915542186826	23.92800596586896
40-41	24.40909506164033	25.723308692218836	25.91576655891654	23.951829687224286
42-43	24.200354445074577	25.717729325069534	25.87597997258327	24.205936257272622
44-45	24.188397850504	25.783371282766065	25.95042089228349	24.07780997444644
46-47	24.343666475941152	25.700493012391934	25.802371789344974	24.15346872232194
48-49	24.126214223828672	25.796308392748337	25.87155198211209	24.2059254013109
50-51	24.196028146891788	25.84112274057551	25.908072715416363	24.05477639711634
52-53	24.314552519398955	25.741685988937906	25.90197553534749	24.04178595631565
54-55	23.965677190142085	25.567978921488248	26.179105900146066	24.2872379882236
56-57	24.051766467685464	25.76778335694393	25.96875562437015	24.211694551000456
58-59	24.115116945532442	25.660887429029383	26.04065156891001	24.18334405652817
60-61	24.25155830957863	25.624962176951975	26.10356287625114	24.019916637218255
62-63	24.139751787470725	25.653305147269776	26.08993933746237	24.117003727797126
64-65	24.002537270073617	25.487680584914123	26.10911107909388	24.400671065918385
66-67	23.97389054079983	25.67455758954493	25.999444439567444	24.3521074300878
68-69	24.045320780525948	25.533461133361246	26.07076677038335	24.35045131572945
70-71	23.960163020470972	25.397837688899727	26.09249121867723	24.54950807195207
72-73	23.9482708922199	25.557822588646662	25.992174425390186	24.501732093743257
74-75	23.833221261381574	25.634664246539486	26.10166728580542	24.43044720627352
76-77	23.918862301682033	25.37277218745107	26.09639639314862	24.61196911771828
78-79	23.853095220102556	25.335601247614157	26.195084109224876	24.61621942305841
80-81	23.928154183168797	25.488070723784528	26.015250294266927	24.56852479877975
82-83	23.896772615485204	25.520633011721188	26.100052291045532	24.482542081748075
84-85	23.947611689064267	25.47826425071934	26.0770823246678	24.497041735548592
86-87	23.7539815259704	25.54692273048315	26.16160510538577	24.537490638160676
88-89	23.726082912830208	25.35438224655379	26.203014763960265	24.716520076655737
90-91	23.757024162255696	25.31458289393525	26.26738350299	24.661009440819058
92-93	23.762780076519725	25.20930957556613	26.37625260353042	24.65165774438373
94-95	23.723096577939135	25.125721689153725	26.39345426976969	24.757727463137453
96-97	23.630235974575893	25.031786407885757	26.443907478380773	24.894070139157577
98-99	23.515611817522046	24.994809338047897	26.460630318860325	25.02894852556973
100-101	23.696748021797166	24.907853246473394	26.554152116560193	24.84124661516925
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	38.5
2	53.5
3	82.5
4	91.0
5	102.0
6	159.5
7	312.5
8	477.0
9	543.0
10	477.0
11	434.0
12	474.5
13	522.5
14	588.0
15	675.0
16	761.5
17	843.0
18	992.0
19	1096.0
20	1157.0
21	1272.5
22	1408.5
23	1601.0
24	1826.5
25	2152.5
26	2669.5
27	3488.5
28	4846.0
29	7102.0
30	10557.5
31	15394.0
32	21962.5
33	28221.5
34	34182.5
35	41484.5
36	51220.5
37	60926.0
38	69519.0
39	78297.5
40	86125.0
41	93750.5
42	101453.0
43	109957.0
44	118621.5
45	126954.0
46	133141.0
47	137687.0
48	142280.0
49	144335.0
50	143878.5
51	142759.0
52	140981.5
53	138768.0
54	135297.5
55	129100.5
56	119457.5
57	106671.0
58	95269.0
59	84140.0
60	70320.5
61	57678.5
62	47100.5
63	36244.5
64	26231.5
65	19230.0
66	14564.5
67	11102.5
68	8451.5
69	6538.5
70	4999.0
71	3676.0
72	2761.5
73	2122.5
74	1649.5
75	1286.5
76	1000.0
77	713.0
78	449.0
79	309.5
80	247.0
81	186.5
82	140.0
83	119.0
84	97.0
85	77.5
86	64.5
87	55.5
88	49.5
89	46.0
90	36.5
91	31.5
92	28.5
93	20.0
94	19.0
95	21.5
96	17.5
97	11.0
98	10.5
99	11.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05287216293135786
2	0.06128790957546156
3	0.051714606701499716
4	0.05912922363329369
5	0.051714606701499716
6	0.019803597121626937
7	0.017425914054891314
8	0.022431562616439988
9	0.02318240990067229
10-11	0.024496392648078815
12-13	0.030002606065782356
14-15	0.04750673337944787
16-17	0.031097591688621126
18-19	0.024856173638440126
20-21	0.029220473478040375
22-23	0.05005648561548673
24-25	0.04522290622324129
26-27	0.03289649664042768
28-29	0.021414790252375415
30-31	0.012733118528439436
32-33	0.00944816165992312
34-35	0.009166593928336008
36-37	0.0013921960061807246
38-39	0.006491700478258435
40-41	0.0032849568685163165
42-43	0.23340400683396167
44-45	0.20704613862705698
46-47	0.2131780581149541
48-49	0.2111288707350701
50-51	0.2094238216937926
52-53	0.21425740108603802
54-55	0.21242721083072177
56-57	0.22347092296963858
58-59	0.22027982201165128
60-61	0.22512904405565154
62-63	0.22231336673978042
64-65	0.22320499788980627
66-67	0.21327191402548312
68-69	0.21181714741228308
70-71	0.19514208064162403
72-73	0.20221255923481155
74-75	0.20254105492166313
76-77	0.20036672632774044
78-79	0.22719387408729036
80-81	0.23565654868665858
82-83	0.23484313079540695
84-85	0.23016597792070992
86-87	0.2475606066720915
88-89	0.24551141929220754
90-91	0.24532370747114948
92-93	0.24809245683175607
94-95	0.25952723526454385
96-97	0.26005908542420836
98-99	0.2493751542756529
100-101	0.2586668894180277
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3196389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.33056648911052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.71459056523636	47.48895839759103
2	11.896305011463873	14.354216409377196
3	3.838443511518973	6.947264144591707
4	1.7274753830958216	4.1687827383265645
5	0.9742922687932146	2.938980225112768
6	0.6186783669706377	2.239512981233782
7	0.40973838189030143	1.730382408424137
8	0.31152504147603166	1.5035585782234109
9	0.22666170430339755	1.2307166119810107
>10	1.2014718647401046	12.833013323121836
>50	0.06287720996832019	2.541654115413432
>100	0.017159877339980836	1.6768499490123716
>500	7.287589895026367E-4	0.30597503636849427
>1k	5.205421351546223E-5	0.040135081222231084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.1285303509679204E-5	0.0	0.0	0.0	0.0
9	3.1285303509679204E-5	0.0	0.0	0.0	3.1285303509679204E-5
10-11	3.1285303509679204E-5	0.0	0.0	3.1285303509679204E-5	3.1285303509679204E-5
12-13	3.1285303509679204E-5	0.0	0.0	3.1285303509679204E-5	3.1285303509679204E-5
14-15	3.1285303509679204E-5	0.0	0.0	3.1285303509679204E-5	9.385591052903761E-5
16-17	3.1285303509679204E-5	0.0	0.0	4.692795526451881E-5	1.5642651754839602E-4
18-19	3.1285303509679204E-5	0.0	0.0	6.257060701935841E-5	2.5028242807743364E-4
20-21	3.1285303509679204E-5	0.0	0.0	6.257060701935841E-5	2.5028242807743364E-4
22-23	3.1285303509679204E-5	0.0	0.0	1.4078386579355643E-4	2.8156773158711287E-4
24-25	3.1285303509679204E-5	0.0	0.0	3.7542364211615045E-4	3.1285303509679204E-4
26-27	4.692795526451881E-5	0.0	0.0	7.664899359871404E-4	3.441383386064712E-4
28-29	6.257060701935841E-5	0.0	0.0	0.0030659597439485617	4.0670894562582963E-4
30-31	6.257060701935841E-5	1.5642651754839602E-5	0.0	0.010949856228387721	4.0670894562582963E-4
32-33	6.257060701935841E-5	3.1285303509679204E-5	0.0	0.028845049835924227	4.0670894562582963E-4
34-35	6.257060701935841E-5	3.1285303509679204E-5	0.0	0.05187103321904812	4.0670894562582963E-4
36-37	7.821325877419801E-5	3.1285303509679204E-5	0.0	0.08892847522626313	4.2235159738066925E-4
38-39	9.385591052903761E-5	3.1285303509679204E-5	0.0	0.14974710524907953	4.536369008903484E-4
40-41	9.385591052903761E-5	4.692795526451881E-5	0.0	0.21909098047828346	4.6927955264518804E-4
42-43	9.385591052903761E-5	6.257060701935841E-5	0.0	0.2919388097005715	4.8492220440002766E-4
44-45	9.385591052903761E-5	6.257060701935841E-5	0.0	0.3815555616040476	5.162075079097069E-4
46-47	9.385591052903761E-5	6.257060701935841E-5	0.0	0.4848439911412534	5.787781149290652E-4
48-49	9.385591052903761E-5	6.257060701935841E-5	0.0	0.5909950259495951	6.413487219484237E-4
50-51	9.385591052903761E-5	6.257060701935841E-5	0.0	0.7079082051652662	6.569913737032633E-4
52-53	9.385591052903761E-5	6.257060701935841E-5	0.0	0.8312035862969119	6.882766772129424E-4
54-55	9.385591052903761E-5	6.257060701935841E-5	0.0	0.96695051822541	6.882766772129424E-4
56-57	9.385591052903761E-5	6.257060701935841E-5	0.0	1.1262396410449416	6.882766772129424E-4
58-59	9.385591052903761E-5	6.257060701935841E-5	0.0	1.2993412253639967	6.882766772129424E-4
60-61	9.385591052903761E-5	6.257060701935841E-5	0.0	1.4853479973807944	6.882766772129424E-4
62-63	9.385591052903761E-5	6.257060701935841E-5	0.0	1.6786911730706118	7.195619807226217E-4
64-65	9.385591052903761E-5	6.257060701935841E-5	0.0	1.886707156106469	7.195619807226217E-4
66-67	9.385591052903761E-5	7.821325877419801E-5	0.0	2.128229699201192	7.195619807226217E-4
68-69	9.385591052903761E-5	9.385591052903761E-5	0.0	2.392465372643943	7.195619807226217E-4
70-71	9.385591052903761E-5	9.385591052903761E-5	0.0	2.6740331042310554	7.508472842323009E-4
72-73	9.385591052903761E-5	9.385591052903761E-5	0.0	2.96669147591235	8.603458465161781E-4
74-75	9.385591052903761E-5	9.385591052903761E-5	0.0	3.272677386888767	8.759884982710177E-4
76-77	9.385591052903761E-5	9.385591052903761E-5	0.0	3.615016820543432	8.759884982710177E-4
78-79	9.385591052903761E-5	9.385591052903761E-5	0.0	3.9949924743202407	8.759884982710177E-4
80-81	9.385591052903761E-5	1.0949856228387722E-4	0.0	4.401670134642561	9.072738017806968E-4
82-83	9.385591052903761E-5	1.2514121403871682E-4	0.0	4.818859656944133	9.385591052903761E-4
84-85	9.385591052903761E-5	1.2514121403871682E-4	0.0	5.264018240583358	9.385591052903761E-4
86-87	9.385591052903761E-5	1.2514121403871682E-4	0.0	5.741478900096327	9.385591052903761E-4
88-89	9.385591052903761E-5	1.2514121403871682E-4	0.0	6.2571858431498795	9.385591052903761E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2205	0.0	45.621647	1
GTATCAA	4015	0.0	44.200764	1
TCAACGC	4815	0.0	35.283466	4
CAACGCA	4910	0.0	34.597	5
TATCAAC	5195	0.0	33.159317	2
AACGCAG	5180	0.0	32.691334	6
ATCAACG	5235	0.0	32.632977	3
GTACATG	8860	0.0	29.509481	1
TACATGG	9145	0.0	28.229273	2
ACGCAGA	6325	0.0	26.328268	7
ACATGGG	9650	0.0	26.30858	3
CGCAGAG	6525	0.0	24.940762	8
CATGGGG	8370	0.0	22.338562	4
GCAGAGT	7805	0.0	19.634766	9
GAGTACA	6490	0.0	19.1558	1
GTATAGG	910	0.0	18.250317	1
GTGTAGC	1810	0.0	18.088985	1
AGAGTAC	6510	0.0	17.96534	10-11
AGTACAT	6560	0.0	17.578709	2
TCTATAC	820	0.0	16.782438	3
>>END_MODULE