FastQCFastQC Report
Mon 15 Aug 2016
SRR2921412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196389
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.050.5514871
GTATCAA41250.041.7115971
TCAACGC51300.033.766124
CAACGCA52600.032.8179175
TATCAAC54600.031.3776382
ATCAACG55800.030.872953
AACGCAG56300.030.6572976
GTACATG86850.026.5426431
TACATGG90250.024.188912
ACGCAGA70200.024.0447397
CGCAGAG72350.022.7387398
ACATGGG92050.022.4785543
CATGGGG78950.019.6562794
GTATATA9500.018.5118731
GCAGAGT85400.018.2061759
AGAGTAC72800.017.07926410-11
ATACCGT3101.1053635E-716.8720026
GAGTACA67450.016.4894491
TATACCG2606.5586137E-616.4611155
TATACTG14300.015.9622955
Produced by FastQC (version 0.11.5)