##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3196389
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43615905323163	33.0	31.0	34.0	29.0	34.0
2	31.5667026134804	34.0	31.0	34.0	29.0	34.0
3	31.645152389149132	34.0	31.0	34.0	28.0	34.0
4	35.12731741975085	37.0	35.0	37.0	33.0	37.0
5	34.87730091675325	37.0	35.0	37.0	32.0	37.0
6	34.83325527650108	37.0	35.0	37.0	32.0	37.0
7	34.70066972449223	37.0	35.0	37.0	32.0	37.0
8	34.66343145343073	37.0	35.0	37.0	32.0	37.0
9	36.29246659277078	39.0	37.0	39.0	32.0	39.0
10-11	36.139821999137155	39.0	37.0	39.0	32.0	39.0
12-13	36.07624807243424	39.0	37.0	39.0	32.0	39.0
14-15	37.33451200714306	40.0	38.0	41.0	32.0	41.0
16-17	37.31305732812871	40.0	38.0	41.0	32.0	41.0
18-19	37.270735195246885	40.0	38.0	41.0	31.0	41.0
20-21	37.23198349762811	40.0	38.0	41.0	31.0	41.0
22-23	37.12298784659815	40.0	38.0	41.0	31.0	41.0
24-25	37.06095769319691	40.0	38.0	41.0	31.0	41.0
26-27	36.92876946454265	40.0	37.0	41.0	30.0	41.0
28-29	36.778553861873505	40.0	37.0	41.0	30.0	41.0
30-31	36.65022264186243	40.0	37.0	41.0	30.0	41.0
32-33	36.612990315008595	40.0	37.0	41.0	30.0	41.0
34-35	36.75259613269849	40.0	37.0	41.0	30.0	41.0
36-37	36.736940653969214	40.0	37.0	41.0	30.0	41.0
38-39	36.65333631169423	40.0	37.0	41.0	30.0	41.0
40-41	36.53942073383433	40.0	37.0	41.0	30.0	41.0
42-43	36.44554511356409	40.0	37.0	41.0	30.0	41.0
44-45	36.32374016429164	40.0	36.5	41.0	29.5	41.0
46-47	36.1800049993915	40.0	36.0	41.0	29.0	41.0
48-49	36.029123801890194	40.0	36.0	41.0	28.0	41.0
50-51	35.87880480129296	40.0	35.5	41.0	28.0	41.0
52-53	35.70940301696696	39.5	35.0	41.0	28.0	41.0
54-55	35.50418362721183	39.0	35.0	41.0	27.0	41.0
56-57	35.238539645831594	39.0	35.0	41.0	26.5	41.0
58-59	35.01529491560633	39.0	35.0	40.5	26.0	41.0
60-61	34.742966203425176	38.0	35.0	40.0	26.0	41.0
62-63	34.44882490835752	37.5	34.0	40.0	26.0	41.0
64-65	34.131201177328535	37.0	34.0	40.0	26.0	41.0
66-67	33.787355199883365	36.5	34.0	39.5	25.0	41.0
68-69	33.417856681398916	36.0	34.0	39.0	24.5	41.0
70-71	33.03750138046402	35.5	33.5	39.0	23.5	40.0
72-73	32.639558264028565	35.0	33.0	37.5	22.5	40.0
74-75	32.23253099043953	35.0	33.0	37.0	21.5	39.0
76-77	29.481608934331835	33.0	29.0	34.5	16.5	36.5
78-79	31.133510814860145	34.5	31.5	36.0	19.5	37.0
80-81	31.32684585637105	35.0	32.0	36.0	20.0	37.0
82-83	31.167506520639385	35.0	32.0	35.0	20.0	37.0
84-85	30.941046286919395	35.0	32.0	35.0	18.5	36.0
86-87	30.698932608014857	35.0	32.0	35.0	17.5	36.0
88-89	30.42659967231773	35.0	32.0	35.0	11.0	36.0
90-91	30.252041287840747	35.0	32.0	35.0	7.5	35.0
92-93	30.11333195052292	34.0	32.0	35.0	4.5	35.0
94-95	30.022568122966263	34.0	32.0	35.0	2.0	35.0
96-97	29.838476011524257	34.0	31.5	35.0	2.0	35.0
98-99	29.57554806376821	34.0	31.0	35.0	2.0	35.0
100-101	28.49679841846534	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58581.0
3	19637.0
4	10674.0
5	7495.0
6	6520.0
7	6520.0
8	6915.0
9	7391.0
10	8207.0
11	8850.0
12	9541.0
13	10010.0
14	10920.0
15	11687.0
16	12474.0
17	13272.0
18	12602.0
19	11609.0
20	11139.0
21	11046.0
22	11755.0
23	13066.0
24	15306.0
25	18678.0
26	22850.0
27	27596.0
28	33152.0
29	39771.0
30	48308.0
31	60980.0
32	78102.0
33	103421.0
34	147967.0
35	221651.0
36	393104.0
37	777116.0
38	824631.0
39	103842.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22175127522322	19.22016316088651	13.397066814238881	26.161018749651387
2	15.081952339685332	20.458363706329365	37.880595800861606	26.5790881531237
3	18.005094172738197	24.118539478587238	28.876938590532948	28.999427758141614
4	13.021158584889015	16.445834548301068	35.27937791983341	35.25362894697651
5	14.295849203510356	37.17847733299035	34.26600059647143	14.259672867027867
6	34.18910218105299	35.34407726823066	16.57990092436801	13.886919626348337
7	29.51473945887637	31.473827003371934	20.461139549478695	18.550293988273005
8	26.948832471301408	35.33465318284379	19.20452995378441	18.511984392070392
9	26.663337910373237	15.551392524501868	18.909275435499246	38.87599412962565
10-11	25.835230430561857	25.281095860034547	27.559554393432883	21.32411931597071
12-13	26.30275417644074	22.46973296683563	27.471153322725506	23.756359533998125
14-15	23.381278251299783	24.130292120101902	24.55554677777769	27.932882850820622
16-17	23.831625414335257	27.36639985483601	25.235946233010104	23.566028497818632
18-19	23.859056785966747	26.789825801348055	26.43473655115108	22.91638086153412
20-21	24.393397253819728	25.808722840650027	26.44566471872598	23.35221518680427
22-23	24.1263754029361	25.62395946791708	26.15160299232116	24.098062136825657
24-25	23.959253332332185	25.833102600321933	26.150513009431116	24.05713105791477
26-27	23.88495814711629	26.074901879195263	26.145686517129885	23.89445345655857
28-29	24.019923706097178	26.157905069860465	26.00342438698545	23.818746837056903
30-31	24.29348672958466	25.89814906946575	25.885992892156402	23.922371308793185
32-33	24.00880691987474	25.95068800736945	25.89028241219364	24.15022266056217
34-35	24.214266706028624	26.022350894303298	26.00848972904864	23.754892670619444
36-37	24.30302903264863	25.867195475363125	26.001332137392236	23.828443354596015
38-39	24.212985181370687	25.98808590462592	25.960554777245022	23.83837413675837
40-41	24.290974269879133	25.84015659677036	25.961643302454814	23.907225830895698
42-43	24.088472103609913	25.852260416472745	25.874334169716345	24.184933310201
44-45	24.065881839082188	25.843429963117735	25.95867922190559	24.132008975894493
46-47	24.194330086248918	25.83939854159666	25.822096193546006	24.144175178608414
48-49	24.05018793645986	25.914533888480918	25.864205777986232	24.17107239707299
50-51	24.061733317960908	25.975787806737344	25.874479987873094	24.087998887428647
52-53	24.231556247109005	25.833713585182334	25.827863155637225	24.10686701207144
54-55	23.85234491355699	25.708209148891335	26.080336208014476	24.359109729537195
56-57	23.951733850990607	25.86942065008872	25.91736829532746	24.26147720359321
58-59	24.033013473654833	25.777301570763516	25.967659223507226	24.22202573207442
60-61	24.109597827379275	25.848820765047837	25.91884272904191	24.12273867853098
62-63	24.03111969671344	25.825308581807406	25.944825214551837	24.198746506927318
64-65	23.82866082904859	25.647468291243293	25.98991378773449	24.533957091973623
66-67	23.868091422437164	25.87773567426434	25.857345506925693	24.396827396372803
68-69	23.897206952864952	25.762117435252858	25.88015885521103	24.460516756671165
70-71	23.840840927781084	25.59667046682478	25.87851335310876	24.683975252285375
72-73	23.904238343904915	25.80294518852505	25.708860256415473	24.583956211154565
74-75	23.751256426079586	25.86583872934173	25.763509631452525	24.619395213126165
76-77	23.81963783168209	25.579623304051207	25.88131000841486	24.71942885585184
78-79	23.755468864591	25.588189115290373	25.86047200396856	24.79587001615007
80-81	23.803691121281624	25.759718077692035	25.681884557222272	24.754706243804073
82-83	23.806275398374975	25.816882459140817	25.71255481777632	24.664287324707892
84-85	23.809491768576255	25.75754635848223	25.690635918325448	24.74232595461607
86-87	23.68079760832727	25.828947681532792	25.73083411715639	24.759420592983542
88-89	23.592324970822524	25.662141680901847	25.776138133334754	24.969395214940874
90-91	23.60024682786644	25.588946857307505	25.78037655687681	25.030429757949246
92-93	23.59285285223245	25.57372851618929	25.846166415154226	24.98725221642403
94-95	23.551463624562725	25.499630969063663	25.83789107658479	25.111014329788823
96-97	23.41140017183862	25.46970890688487	25.8392307687494	25.27966015252711
98-99	23.299373174378786	25.436690393257173	25.831923258560913	25.43201317380313
100-101	23.321942692221956	25.450832398018044	25.84520564552398	25.38201926423602
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	35.5
2	43.0
3	59.0
4	57.0
5	63.0
6	115.0
7	251.0
8	385.0
9	412.5
10	336.0
11	266.0
12	275.0
13	289.5
14	309.5
15	359.5
16	422.5
17	482.5
18	555.5
19	628.0
20	691.0
21	762.0
22	920.5
23	1196.0
24	1504.0
25	1921.0
26	2570.0
27	3573.0
28	5119.5
29	7604.5
30	11364.5
31	16350.5
32	22740.0
33	28997.0
34	34968.0
35	42244.5
36	51448.0
37	60798.0
38	69542.5
39	78115.0
40	85898.0
41	93628.0
42	101715.5
43	110154.0
44	118537.0
45	126762.5
46	132796.0
47	137658.0
48	142930.5
49	145720.5
50	145115.5
51	144034.5
52	142436.5
53	140049.0
54	136907.0
55	130091.5
56	119729.5
57	107143.0
58	96055.5
59	84814.0
60	70290.5
61	57084.5
62	46297.5
63	35101.0
64	25031.5
65	18192.0
66	13546.5
67	10279.5
68	7824.0
69	6004.5
70	4583.5
71	3306.5
72	2363.0
73	1894.5
74	1487.5
75	1065.0
76	783.5
77	512.5
78	291.0
79	161.5
80	99.5
81	68.0
82	44.0
83	26.0
84	17.5
85	16.5
86	14.0
87	11.5
88	6.5
89	3.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.5
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16215172809066733
2	5.944207666839049E-4
3	0.00588163705981969
4	0.004536369008903485
5	0.02909533226400166
6	0.0013452680509162059
7	9.385591052903761E-5
8	0.0020335447281291485
9	0.0
10-11	0.11176674678832896
12-13	2.189971245677544E-4
14-15	0.005834709104555171
16-17	1.2514121403871682E-4
18-19	2.9721038334195243E-4
20-21	6.882766772129424E-4
22-23	2.3463977632259402E-4
24-25	0.001689406389522677
26-27	0.002315112459716261
28-29	0.006147562139651964
30-31	0.015110801595175055
32-33	0.015423654630271849
34-35	0.012811331787213633
36-37	6.882766772129424E-4
38-39	2.189971245677544E-4
40-41	0.004833579392245437
42-43	0.008931954152013413
44-45	0.008540887858142422
46-47	0.009104023321316648
48-49	0.011325279870503871
50-51	0.005928565015084209
52-53	0.0013296253991613661
54-55	0.0010793429710839325
56-57	0.006084991532632605
58-59	0.009385591052903762
60-61	0.007790040573910122
62-63	0.018755539454052682
64-65	0.035540104786995576
66-67	0.038214998237073146
68-69	0.02817241581046612
70-71	0.04212566117578305
72-73	0.027280784660440264
74-75	0.025560092967407907
76-77	0.2497192926142594
78-79	0.07147127586786214
80-81	0.054749281141938604
82-83	0.021508646162904453
84-85	0.03356913066588579
86-87	0.08262448656906278
88-89	0.2681306937297056
90-91	0.3242096002708056
92-93	0.05368558082260951
94-95	0.005631354631742257
96-97	0.011481706388052269
98-99	0.0014234813096904036
100-101	0.001611193130748479
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3196389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.520892212443776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18858587157715	46.53854391905547
2	12.1079709638888	14.413544693060484
3	3.9295209047250723	7.016657706500566
4	1.8150499582907134	4.321335717104885
5	1.0129292510763173	3.0145226386072435
6	0.612773555009465	2.188369723101261
7	0.43542706570719564	1.8141905211037064
8	0.31040767548295245	1.4780593435468832
9	0.23811349761409073	1.2755455043234654
>10	1.2671741945178745	13.348759209387975
>50	0.06382790221927562	2.556499647643341
>100	0.01742827180488145	1.6892301139497548
>500	7.381622138784887E-4	0.30595198635047394
>1k	5.2725872265177894E-5	0.038789276264397765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1285303509679204E-5
2	0.0	0.0	0.0	0.0	3.1285303509679204E-5
3	0.0	0.0	0.0	0.0	3.1285303509679204E-5
4	0.0	0.0	0.0	0.0	3.1285303509679204E-5
5	0.0	0.0	0.0	0.0	3.1285303509679204E-5
6	0.0	0.0	0.0	0.0	3.1285303509679204E-5
7	0.0	0.0	0.0	0.0	3.1285303509679204E-5
8	0.0	0.0	0.0	0.0	3.1285303509679204E-5
9	0.0	0.0	0.0	0.0	6.257060701935841E-5
10-11	0.0	0.0	0.0	0.0	6.257060701935841E-5
12-13	0.0	0.0	0.0	0.0	6.257060701935841E-5
14-15	0.0	0.0	0.0	0.0	7.821325877419801E-5
16-17	0.0	0.0	0.0	4.692795526451881E-5	9.385591052903761E-5
18-19	0.0	0.0	0.0	6.257060701935841E-5	1.0949856228387722E-4
20-21	0.0	0.0	0.0	6.257060701935841E-5	1.4078386579355643E-4
22-23	0.0	1.5642651754839602E-5	0.0	1.4078386579355643E-4	2.0335447281291484E-4
24-25	0.0	4.692795526451881E-5	0.0	3.9106629387099007E-4	2.6592507983227325E-4
26-27	0.0	6.257060701935841E-5	0.0	7.977752394968196E-4	2.815677315871128E-4
28-29	0.0	6.257060701935841E-5	0.0	0.00312853035096792	2.815677315871128E-4
30-31	0.0	6.257060701935841E-5	0.0	0.011012426835407079	2.815677315871128E-4
32-33	0.0	7.821325877419801E-5	0.0	0.029486398557872648	3.1285303509679204E-4
34-35	0.0	9.385591052903761E-5	0.0	0.052903448234867534	3.441383386064712E-4
36-37	0.0	9.385591052903761E-5	0.0	0.09047709774999226	3.441383386064712E-4
38-39	0.0	9.385591052903761E-5	0.0	0.1513739410315828	3.441383386064712E-4
40-41	0.0	9.385591052903761E-5	0.0	0.22145302089326424	3.441383386064712E-4
42-43	0.0	9.385591052903761E-5	0.0	0.2957399740769975	3.5978099036131084E-4
44-45	0.0	9.385591052903761E-5	0.0	0.3865768528173511	3.9106629387099007E-4
46-47	0.0	9.385591052903761E-5	0.0	0.49047534577299573	4.0670894562582963E-4
48-49	0.0	1.2514121403871682E-4	0.0	0.5968297350541502	4.3799424913550886E-4
50-51	0.0	1.2514121403871682E-4	0.0	0.713680343662802	4.3799424913550886E-4
52-53	0.0	1.2514121403871682E-4	0.0	0.8367097997146155	4.8492220440002766E-4
54-55	0.0	1.5642651754839602E-4	0.0	0.9730042244545329	5.318501596645465E-4
56-57	0.0	1.5642651754839602E-4	0.0	1.1327626268267097	5.318501596645465E-4
58-59	0.0	1.5642651754839602E-4	0.0	1.3065681304747327	5.318501596645465E-4
60-61	0.0	1.5642651754839602E-4	0.0	1.4930598246959303	5.47492811419386E-4
62-63	0.0	1.5642651754839602E-4	0.0	1.686684568117335	5.631354631742256E-4
64-65	0.0	1.5642651754839602E-4	0.0	1.8948413350189854	5.787781149290652E-4
66-67	0.0	1.5642651754839602E-4	0.0	2.136520304631257	5.944207666839049E-4
68-69	0.0	1.5642651754839602E-4	0.0	2.40108447376086	5.944207666839049E-4
70-71	0.0	1.5642651754839602E-4	0.0	2.684544966210308	5.944207666839049E-4
72-73	0.0	1.5642651754839602E-4	0.0	2.979909516645189	6.726340254581029E-4
74-75	0.0	1.5642651754839602E-4	0.0	3.2885390357681743	6.882766772129424E-4
76-77	0.0	1.5642651754839602E-4	0.0	3.6337723599974847	6.882766772129424E-4
78-79	0.0	1.5642651754839602E-4	0.0	4.016438549876126	6.882766772129424E-4
80-81	0.0	1.5642651754839602E-4	0.0	4.428340855884562	7.664899359871404E-4
82-83	0.0	1.5642651754839602E-4	0.0	4.850410885533645	9.07273801780697E-4
84-85	0.0	1.5642651754839602E-4	0.0	5.300496904475644	9.229164535355366E-4
86-87	0.0	1.5642651754839602E-4	0.0	5.783557633316846	9.542017570452157E-4
88-89	0.0	1.720691693032356E-4	0.0	6.3063350549635855	9.698444088000553E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2360	0.0	50.551487	1
GTATCAA	4125	0.0	41.711597	1
TCAACGC	5130	0.0	33.76612	4
CAACGCA	5260	0.0	32.817917	5
TATCAAC	5460	0.0	31.377638	2
ATCAACG	5580	0.0	30.87295	3
AACGCAG	5630	0.0	30.657297	6
GTACATG	8685	0.0	26.542643	1
TACATGG	9025	0.0	24.18891	2
ACGCAGA	7020	0.0	24.044739	7
CGCAGAG	7235	0.0	22.738739	8
ACATGGG	9205	0.0	22.478554	3
CATGGGG	7895	0.0	19.656279	4
GTATATA	950	0.0	18.511873	1
GCAGAGT	8540	0.0	18.206175	9
AGAGTAC	7280	0.0	17.079264	10-11
ATACCGT	310	1.1053635E-7	16.872002	6
GAGTACA	6745	0.0	16.489449	1
TATACCG	260	6.5586137E-6	16.461115	5
TATACTG	1430	0.0	15.962295	5
>>END_MODULE