FastQCFastQC Report
Mon 15 Aug 2016
SRR2921411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933059
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.047.9889031
GTATCAA39850.046.674541
TCAACGC48800.036.9513744
CAACGCA49400.036.3098345
ATCAACG52600.034.2812963
AACGCAG52850.034.110996
TATCAAC53250.033.952532
ACGCAGA61750.028.3543177
GTACATG86350.026.4854341
CGCAGAG65000.026.1362888
TACATGG89000.025.2729032
ACATGGG92350.023.7391153
CATGGGG83150.021.5722964
GCAGAGT76700.021.0357429
ATGGGGG49050.018.6714345
AGAGTAC64150.018.45676210-11
GAGTACA65600.017.7208541
CAGAGTA72100.017.07983610-11
GTATAGG7350.016.78451
GTATATA7750.016.530441
Produced by FastQC (version 0.11.5)