##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921411_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2933059
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.348104487499228	31.0	31.0	34.0	26.0	34.0
2	30.417034911333186	33.0	31.0	34.0	26.0	34.0
3	30.465932325261782	33.0	31.0	34.0	26.0	34.0
4	33.909830658026316	37.0	35.0	37.0	30.0	37.0
5	33.892719171349775	37.0	35.0	37.0	30.0	37.0
6	33.93110298838175	37.0	35.0	37.0	30.0	37.0
7	33.84558237662454	37.0	35.0	37.0	29.0	37.0
8	33.81124348333941	37.0	35.0	37.0	29.0	37.0
9	35.37052715270985	39.0	37.0	39.0	30.0	39.0
10-11	35.264697368856204	39.0	37.0	39.0	29.0	39.0
12-13	35.15332405519289	39.0	35.0	39.0	28.0	39.0
14-15	36.29791678244454	40.0	37.0	41.0	27.5	41.0
16-17	36.24494341914023	40.0	37.0	41.0	27.0	41.0
18-19	36.187931269026635	40.0	37.0	41.0	27.0	41.0
20-21	36.122138865941665	40.0	37.0	41.0	27.0	41.0
22-23	36.00119210012482	40.0	36.5	41.0	27.0	41.0
24-25	35.90468125598564	40.0	36.0	41.0	27.0	41.0
26-27	35.76248295721293	40.0	36.0	41.0	27.0	41.0
28-29	35.62584591718067	40.0	36.0	41.0	26.0	41.0
30-31	35.48626434040365	40.0	36.0	41.0	25.0	41.0
32-33	35.3221033057978	39.5	35.0	41.0	25.0	41.0
34-35	35.158297872630584	39.0	35.0	41.0	24.0	41.0
36-37	34.99626004795675	39.0	35.0	41.0	24.0	41.0
38-39	35.23634744476671	39.5	35.0	41.0	24.5	41.0
40-41	35.365157843739254	40.0	35.0	41.0	24.5	41.0
42-43	35.215382472701705	40.0	35.0	41.0	24.0	41.0
44-45	35.10293076272929	40.0	35.0	41.0	23.0	41.0
46-47	34.96040328544363	40.0	35.0	41.0	23.0	41.0
48-49	34.82136397528996	39.5	35.0	41.0	22.0	41.0
50-51	33.11852710770564	37.0	32.5	39.5	18.0	40.0
52-53	33.27891528946401	37.5	33.5	39.5	18.0	40.0
54-55	34.13265553130708	39.0	34.0	40.5	18.5	41.0
56-57	34.138747464677664	39.0	34.0	41.0	18.0	41.0
58-59	33.962466148822784	38.0	34.0	41.0	18.0	41.0
60-61	33.712177116109835	38.0	34.0	40.0	16.0	41.0
62-63	33.42358864925663	37.0	34.0	40.0	13.5	41.0
64-65	33.12815545135642	37.0	33.5	40.0	12.0	41.0
66-67	32.782966691089406	36.0	33.0	39.5	8.0	41.0
68-69	32.41621699393023	36.0	33.0	39.0	7.0	41.0
70-71	32.034825927470266	35.0	32.5	39.0	3.5	40.5
72-73	31.680961412641203	35.0	32.0	37.5	2.0	40.0
74-75	31.285762918509313	35.0	32.0	37.0	2.0	39.0
76-77	30.913521855509895	35.0	32.0	36.5	2.0	39.0
78-79	30.579608354281316	35.0	31.5	36.0	2.0	38.5
80-81	30.24016888170337	35.0	31.0	36.0	2.0	37.0
82-83	29.955197116730346	35.0	31.0	35.0	2.0	37.0
84-85	29.732361503808825	35.0	31.0	35.0	2.0	36.0
86-87	29.518152720419195	35.0	31.0	35.0	2.0	36.0
88-89	29.329361768719963	35.0	31.0	35.0	2.0	36.0
90-91	29.094977973508204	35.0	30.5	35.0	2.0	35.5
92-93	28.915954469378214	34.0	30.0	35.0	2.0	35.0
94-95	28.7255812106064	34.0	30.0	35.0	2.0	35.0
96-97	28.489026303255407	34.0	30.0	35.0	2.0	35.0
98-99	28.273954086842437	34.0	30.0	35.0	2.0	35.0
100-101	27.247861362488784	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110444.0
3	18221.0
4	12254.0
5	9152.0
6	8225.0
7	8352.0
8	8698.0
9	9316.0
10	10037.0
11	10623.0
12	10683.0
13	10783.0
14	11251.0
15	11861.0
16	11975.0
17	12126.0
18	11564.0
19	11058.0
20	11090.0
21	11850.0
22	13342.0
23	15097.0
24	17926.0
25	21004.0
26	25084.0
27	30077.0
28	35587.0
29	42169.0
30	50600.0
31	61577.0
32	77434.0
33	100289.0
34	142507.0
35	208597.0
36	351775.0
37	637807.0
38	669451.0
39	113158.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58847360983052	17.97815052038083	13.59331396509009	24.840061904698555
2	16.18115569498178	19.34362632740427	38.92127220669862	25.553945770915327
3	19.303940279263006	23.376466113734782	28.821395496385133	28.49819811061708
4	13.71929244052076	15.792064431508262	35.81212252493191	34.67652060303907
5	15.19450936731244	36.442945419765564	33.62060849668654	14.741936716235445
6	34.12098443714176	34.28971079524742	17.162450951540958	14.426853816069857
7	29.798102933438997	30.899161265661267	20.672174117934293	18.630561682965443
8	27.180809598474458	34.03504081547749	19.669554566020544	19.114595020027515
9	27.043497772527754	15.688572644512888	19.028382879700132	38.23954670325923
10-11	26.06371043205212	24.909511721360893	27.37733356431403	21.64944428227296
12-13	26.605678389432036	22.255412425409432	27.2115085390693	23.927400646089232
14-15	23.898527777531434	23.85427105511437	24.354448764188586	27.89275240316561
16-17	24.228694184356964	26.76081483902883	25.185829755371973	23.824661221242238
18-19	24.298865269970058	26.304562706328312	26.203773976114555	23.192798047587083
20-21	24.865561580614198	25.377993756075668	26.096715178799645	23.65972948451049
22-23	24.67767229227585	25.215861540258878	25.773357108077015	24.333109059388253
24-25	24.443895677230284	25.46832620239141	25.796517799158476	24.291260321219827
26-27	24.288798061092372	25.644740842649966	25.8380450086992	24.228416087558465
28-29	24.413535299903785	25.795512342770955	25.67326026512503	24.117692092200233
30-31	24.70334662211801	25.47545780326381	25.58733524205814	24.233860332560035
32-33	24.470695020835315	25.492275026843302	25.54463183327929	24.492398119042093
34-35	24.693946685083766	25.596349404140746	25.59815656229419	24.111547348481295
36-37	24.69261657556326	25.580881155872483	25.58062544695787	24.14587682160638
38-39	24.604030529874372	25.58034549834377	25.601076080897005	24.21454789088485
40-41	24.68439819322952	25.415261946260582	25.675510268097028	24.22482959241287
42-43	24.54221621505272	25.377629982578675	25.569507115614513	24.51064668675409
44-45	24.51230394680999	25.4602932247809	25.591679924033794	24.435722904375314
46-47	24.649800619278384	25.47217457440196	25.410841112173284	24.467183694146367
48-49	24.430686377036746	25.57967231022979	25.523617550775928	24.466023761957537
50-51	24.495979294515333	25.529340654855016	25.668314299392303	24.306365751237347
52-53	24.65753775616135	25.428391649526898	25.603273618288974	24.310796976022775
54-55	24.356275331303127	25.271609640455615	25.777663533683693	24.59445149455756
56-57	24.35606678515517	25.441457938146268	25.665481496918076	24.536993779780484
58-59	24.486344160131047	25.377135873450218	25.763115439438856	24.37340452697988
60-61	24.550929400796214	25.330010819578824	25.839849735284158	24.2792100443408
62-63	24.441442315510276	25.358057681415097	25.870581330439997	24.329918672634637
64-65	24.35081905607844	25.19872572297286	25.81838990143997	24.632065319508737
66-67	24.363720582757185	25.39158514033733	25.644213001273986	24.6004812756315
68-69	24.377392108426935	25.317762179524294	25.73435134738611	24.57049436466266
70-71	24.310166758084314	25.142061658072496	25.81591104231829	24.7318605415249
72-73	24.315394733561295	25.33469002554557	25.660200518562466	24.68971472233067
74-75	24.241617431950207	25.452249227429807	25.699710782509644	24.606422558110346
76-77	24.33415647980217	25.13254024366502	25.74853387708818	24.78476939944463
78-79	24.243407763349957	25.122099334733505	25.821444830027783	24.813048071888755
80-81	24.313581050053575	25.32190622881634	25.6611181419606	24.70339457916949
82-83	24.283759707820256	25.34237998570808	25.780157600624705	24.593702705846958
84-85	24.350289840069507	25.333409179971433	25.724395182850184	24.591905797108875
86-87	24.18023767981635	25.43342497995871	25.69572158561647	24.690615754608473
88-89	24.163111092962826	25.24205892448288	25.783010710544662	24.811819272009632
90-91	24.195144186799844	25.152044218868504	25.811373763532863	24.841437830798792
92-93	24.21659586929727	25.15806818348792	25.927948997190086	24.697386950024722
94-95	24.145234555235238	25.084955796670666	25.95663379732718	24.81317585076692
96-97	24.136051380844826	25.010534604252598	25.94952412296216	24.90388989194042
98-99	23.994069125983746	24.937520962321557	25.92956523269437	25.13884467900033
100-101	24.22076061289622	24.843352416351667	25.979430803966203	24.956456166785912
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	18.0
2	26.0
3	41.0
4	47.0
5	62.5
6	100.5
7	192.0
8	297.5
9	334.0
10	317.5
11	312.5
12	332.5
13	380.0
14	455.0
15	516.0
16	593.0
17	703.5
18	791.0
19	859.0
20	925.0
21	1008.0
22	1095.5
23	1233.5
24	1390.0
25	1611.5
26	2027.0
27	2658.0
28	3617.0
29	5216.5
30	7681.5
31	11554.5
32	16997.5
33	22399.0
34	28090.0
35	34811.5
36	43043.5
37	52057.0
38	60803.5
39	68769.5
40	76086.5
41	83049.5
42	90077.5
43	98057.0
44	106033.5
45	114112.5
46	119915.0
47	124477.0
48	129635.5
49	131989.5
50	131099.0
51	129660.5
52	128950.0
53	127736.0
54	126182.0
55	122199.0
56	114033.5
57	103516.0
58	93600.5
59	82796.0
60	69149.0
61	57137.0
62	46923.5
63	36331.5
64	26920.5
65	20080.0
66	15440.5
67	12227.5
68	9730.5
69	7693.0
70	5911.5
71	4470.0
72	3427.5
73	2650.0
74	2114.0
75	1681.5
76	1279.0
77	892.5
78	596.5
79	421.5
80	298.5
81	236.5
82	182.0
83	129.5
84	98.0
85	75.5
86	58.5
87	54.0
88	46.0
89	41.5
90	35.0
91	23.0
92	18.5
93	17.5
94	19.0
95	16.5
96	12.0
97	11.0
98	9.5
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0496410061986479
2	0.05727808407536295
3	0.04820905409676382
4	0.053664109722988865
5	0.04687938428787147
6	0.017047048831953262
7	0.014524085604824178
8	0.02021779991469657
9	0.02001323532871313
10-11	0.02164975201658064
12-13	0.02732641927762108
14-15	0.04435642106074238
16-17	0.029747100211758443
18-19	0.023439692143935736
20-21	0.026201314054712165
22-23	0.04693052543436733
24-25	0.042106210614924554
26-27	0.03042898216503657
28-29	0.02023484696352852
30-31	0.013143274649435963
32-33	0.010160041103844143
34-35	0.009461112101734061
36-37	0.001244434564732588
38-39	0.006614254946797865
40-41	0.0031025628874154936
42-43	0.21090608814892572
44-45	0.18552303243814733
46-47	0.1915917818223227
48-49	0.18971660645080785
50-51	0.18838693664191547
52-53	0.19283621638705528
54-55	0.19106332330853215
56-57	0.20154725834018342
58-59	0.19881973052707091
60-61	0.20313263388155506
62-63	0.20037101197077864
64-65	0.20146202309602365
66-67	0.19196681689662567
68-69	0.19073942938072505
70-71	0.1740503685742428
72-73	0.1824034225018999
74-75	0.18177268169511762
76-77	0.17964180059112347
78-79	0.20580902054817174
80-81	0.21394046284101345
82-83	0.2124232754949696
84-85	0.20797399574982978
86-87	0.22505513867944696
88-89	0.22333338674741968
90-91	0.222481034305822
92-93	0.22611205570702808
94-95	0.23792566054757167
96-97	0.23824955447537877
98-99	0.2276633371507358
100-101	0.23813022513355508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2933059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.34804334383281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64648078902127	49.65802236417645
2	11.358424743660674	14.163511164708375
3	3.6986276691862265	6.918065946933664
4	1.710420092281117	4.265653841988224
5	0.9415398164028042	2.935158264151321
6	0.5863884830307906	2.1936104733796857
7	0.40284208550711587	1.7581491065542365
8	0.298373300078347	1.4882393156741769
9	0.2148635319796726	1.205668872438987
>10	1.0810261745723013	11.649282863183402
>50	0.04649518942131577	1.9641446261489361
>100	0.013531608659645377	1.4072561395367738
>500	9.865161987377088E-4	0.3932370211257562
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	6.818819532781304E-5
14-15	0.0	0.0	0.0	0.0	1.8751753715148586E-4
16-17	0.0	0.0	0.0	0.0	2.3865868364734565E-4
18-19	0.0	6.818819532781304E-5	0.0	0.0	2.8979983014320546E-4
20-21	0.0	6.818819532781304E-5	0.0	0.0	3.750350743029717E-4
22-23	0.0	6.818819532781304E-5	0.0	1.704704883195326E-5	3.92082123134925E-4
24-25	0.0	6.818819532781304E-5	0.0	2.0456458598343914E-4	4.091291719668783E-4
26-27	0.0	1.0228229299171957E-4	0.0	3.5798802547101847E-4	4.432232696307848E-4
28-29	0.0	1.0228229299171957E-4	0.0	0.0017047048831953261	4.432232696307848E-4
30-31	0.0	1.0228229299171957E-4	0.0	0.0059494200423516885	4.432232696307848E-4
32-33	0.0	1.0228229299171957E-4	0.0	0.01755846029691186	4.432232696307848E-4
34-35	0.0	1.0228229299171957E-4	0.0	0.03337812161296448	4.432232696307848E-4
36-37	0.0	1.0228229299171957E-4	0.0	0.05659620212208483	4.432232696307848E-4
38-39	0.0	1.0228229299171957E-4	0.0	0.09657153163301523	5.625526114544576E-4
40-41	0.0	1.3637639065562608E-4	0.0	0.14520676195057788	6.136937579503174E-4
42-43	0.0	1.3637639065562608E-4	0.0	0.19801851923196906	6.136937579503174E-4
44-45	0.0	1.3637639065562608E-4	0.0	0.261893811205298	6.477878556142239E-4
46-47	0.0	1.3637639065562608E-4	0.0	0.3386396250467515	7.159760509420369E-4
48-49	0.0	1.3637639065562608E-4	0.0	0.41504449791156606	7.500701486059434E-4
50-51	0.0	1.3637639065562608E-4	0.0	0.4979272493325228	7.500701486059434E-4
52-53	0.0	1.3637639065562608E-4	0.0	0.5899983600739024	8.012112951018033E-4
54-55	0.0	1.3637639065562608E-4	0.0	0.6968492621525855	8.523524415976631E-4
56-57	0.0	1.3637639065562608E-4	0.0	0.8218552712372986	8.864465392615696E-4
58-59	0.0	1.3637639065562608E-4	0.0	0.9526061357783802	9.034935880935229E-4
60-61	0.0	1.3637639065562608E-4	0.0	1.0859992928884146	9.205406369254761E-4
62-63	0.0	1.3637639065562608E-4	0.0	1.2302002789579072	9.546347345893826E-4
64-65	0.0	1.3637639065562608E-4	0.0	1.3906300555154192	9.716817834213359E-4
66-67	0.0	1.3637639065562608E-4	0.0	1.5878132693546227	9.887288322532892E-4
68-69	0.0	1.3637639065562608E-4	0.0	1.7983443224292455	9.887288322532892E-4
70-71	0.0	1.3637639065562608E-4	0.0	2.014074725397614	0.0010228229299171958
72-73	0.0	1.3637639065562608E-4	0.0	2.2418914859878374	0.0012955757112284479
74-75	0.0	1.3637639065562608E-4	0.0	2.495108349337671	0.0013467168577243076
76-77	0.0	1.3637639065562608E-4	0.0	2.790670081986077	0.0013637639065562609
78-79	0.0	1.3637639065562608E-4	0.0	3.116013008943905	0.0013978580042201675
80-81	0.0	1.3637639065562608E-4	0.0	3.4479360967508668	0.0014489991507160272
82-83	0.0	1.3637639065562608E-4	0.0	3.7905817782731273	0.001500140297211887
84-85	0.0	1.3637639065562608E-4	0.0	4.16169603134475	0.001500140297211887
86-87	0.0	1.3637639065562608E-4	0.0	4.577950869723384	0.0015171873460438402
88-89	0.0	1.3637639065562608E-4	0.0	5.026117783515436	0.0015342343948757935
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2195	0.0	47.988903	1
GTATCAA	3985	0.0	46.67454	1
TCAACGC	4880	0.0	36.951374	4
CAACGCA	4940	0.0	36.309834	5
ATCAACG	5260	0.0	34.281296	3
AACGCAG	5285	0.0	34.11099	6
TATCAAC	5325	0.0	33.95253	2
ACGCAGA	6175	0.0	28.354317	7
GTACATG	8635	0.0	26.485434	1
CGCAGAG	6500	0.0	26.136288	8
TACATGG	8900	0.0	25.272903	2
ACATGGG	9235	0.0	23.739115	3
CATGGGG	8315	0.0	21.572296	4
GCAGAGT	7670	0.0	21.035742	9
ATGGGGG	4905	0.0	18.671434	5
AGAGTAC	6415	0.0	18.456762	10-11
GAGTACA	6560	0.0	17.720854	1
CAGAGTA	7210	0.0	17.079836	10-11
GTATAGG	735	0.0	16.7845	1
GTATATA	775	0.0	16.53044	1
>>END_MODULE