FastQCFastQC Report
Mon 15 Aug 2016
SRR2921411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933059
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.047.2108351
GTATCAA40400.041.287181
TCAACGC50350.032.045084
CAACGCA51200.031.472985
TATCAAC53000.030.6218972
ATCAACG53650.030.1624433
AACGCAG55200.029.0191126
GTACATG83050.023.5175291
ACGCAGA66900.023.4466697
CGCAGAG69850.022.048148
TACATGG85750.021.5830252
ACATGGG89200.020.269453
GGACCGT4700.019.2153846
CATGGGG79200.018.3947984
GCAGAGT81200.017.7955289
AGAGTAC67100.017.78052310-11
TATACCG2657.89056E-616.1437635
CTATACT10350.016.0475794
TAAGTAC17850.015.6854023
CAGAGTA74550.015.68486410-11
Produced by FastQC (version 0.11.5)