##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2933059
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234224064364202	33.0	31.0	34.0	28.0	34.0
2	31.37399145397348	34.0	31.0	34.0	28.0	34.0
3	31.4472978552426	34.0	31.0	34.0	28.0	34.0
4	34.926850772521114	37.0	35.0	37.0	32.0	37.0
5	34.64637567808899	37.0	35.0	37.0	32.0	37.0
6	34.5956975294394	37.0	35.0	37.0	32.0	37.0
7	34.455618178836495	37.0	35.0	37.0	31.0	37.0
8	34.41333331514981	37.0	35.0	37.0	31.0	37.0
9	36.021760557833986	39.0	37.0	39.0	31.0	39.0
10-11	35.861991524889206	39.0	37.0	39.0	30.0	39.0
12-13	35.79145867846504	39.0	37.0	39.0	30.0	39.0
14-15	37.017494363393304	40.0	38.0	41.0	31.0	41.0
16-17	36.99099063469231	40.0	38.0	41.0	31.0	41.0
18-19	36.94363222833226	40.0	38.0	41.0	30.5	41.0
20-21	36.901088079032846	40.0	37.5	41.0	30.0	41.0
22-23	36.78459962789702	40.0	37.0	41.0	30.0	41.0
24-25	36.71379368775057	40.0	37.0	41.0	30.0	41.0
26-27	36.57752861432381	40.0	37.0	41.0	30.0	41.0
28-29	36.42066525085244	40.0	37.0	41.0	29.0	41.0
30-31	36.28332229252804	40.0	36.5	41.0	29.0	41.0
32-33	36.24926211849131	40.0	36.5	41.0	29.0	41.0
34-35	36.3901997880029	40.0	37.0	41.0	30.0	41.0
36-37	36.369300617546386	40.0	37.0	41.0	29.5	41.0
38-39	36.28376636815011	40.0	37.0	41.0	29.0	41.0
40-41	36.164434298798625	40.0	36.5	41.0	28.0	41.0
42-43	36.06886513363693	40.0	36.0	41.0	28.0	41.0
44-45	35.938221495033005	40.0	36.0	41.0	28.0	41.0
46-47	35.79096823487015	40.0	36.0	41.0	27.0	41.0
48-49	35.637681171773224	40.0	35.0	41.0	27.0	41.0
50-51	35.48310790884193	39.5	35.0	41.0	26.0	41.0
52-53	35.3087464316265	39.0	35.0	41.0	26.0	41.0
54-55	35.09739115374086	39.0	35.0	41.0	26.0	41.0
56-57	34.82331347579438	39.0	35.0	41.0	25.0	41.0
58-59	34.594570889982094	38.0	34.5	40.0	24.5	41.0
60-61	34.315749870698134	38.0	34.0	40.0	23.5	41.0
62-63	34.015285918217124	37.0	34.0	40.0	23.0	41.0
64-65	33.69853163540181	37.0	34.0	40.0	23.0	41.0
66-67	33.357850455786945	36.0	34.0	39.0	22.0	41.0
68-69	32.99644569031854	36.0	33.0	39.0	22.0	40.5
70-71	32.621497726435095	35.0	33.0	38.5	20.5	40.0
72-73	32.225581722017864	35.0	33.0	37.0	20.0	39.5
74-75	31.823818750321763	35.0	32.5	37.0	18.0	39.0
76-77	29.112541888860743	33.0	28.5	34.5	11.0	36.5
78-79	30.75234149739231	34.5	31.5	36.0	12.5	37.0
80-81	30.95489981619872	35.0	32.0	35.5	14.0	37.0
82-83	30.801828909680985	35.0	32.0	35.0	12.0	37.0
84-85	30.582020511691034	35.0	32.0	35.0	7.5	36.0
86-87	30.34620050261519	35.0	32.0	35.0	6.0	36.0
88-89	30.079917928688104	35.0	31.0	35.0	2.0	36.0
90-91	29.90215982699291	34.0	31.0	35.0	2.0	35.0
92-93	29.763991961975535	34.0	31.0	35.0	2.0	35.0
94-95	29.67196807155942	34.0	31.0	35.0	2.0	35.0
96-97	29.48388985697185	34.0	31.0	35.0	2.0	35.0
98-99	29.219261187722445	34.0	31.0	35.0	2.0	35.0
100-101	28.1406354253358	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66509.0
3	21534.0
4	11549.0
5	8011.0
6	7137.0
7	6864.0
8	7012.0
9	7510.0
10	8282.0
11	8989.0
12	9305.0
13	9726.0
14	10462.0
15	11297.0
16	11749.0
17	12449.0
18	11956.0
19	10883.0
20	10151.0
21	10200.0
22	10891.0
23	12524.0
24	14799.0
25	17755.0
26	21246.0
27	25608.0
28	31007.0
29	37534.0
30	45788.0
31	56724.0
32	72536.0
33	95781.0
34	138996.0
35	209345.0
36	370600.0
37	712956.0
38	716206.0
39	81188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.97568949475463	19.79394115287108	14.2159776698449	26.014391682529386
2	15.100240500830878	20.86878282849979	37.080973158961704	26.950003511707628
3	17.69544715280832	24.67075137492371	28.59036649857308	29.043434973694886
4	13.324702574668734	17.194053300446893	34.72637005906398	34.75487406582039
5	14.844650002966972	36.822618015593314	33.739797195494845	14.592934785944871
6	34.08403826634722	34.97816756170515	16.800902550646583	14.13689162130104
7	29.492627993678944	31.18945945439141	20.736638078726788	18.581274473202853
8	27.111403265323382	34.57270059822666	19.394190661990685	18.921705474459273
9	26.783777619202343	15.599924856608748	18.933713914380856	38.68258360980805
10-11	25.906297742260858	25.299087426920693	27.27253801591504	21.52207681490341
12-13	26.454429084814286	22.476811250543165	27.224480868802246	23.844278795840303
14-15	23.730677853758593	24.0726767736005	24.277576081590123	27.91906929105078
16-17	24.159476204308685	27.030189031766227	25.059221588979668	23.751113174945417
18-19	24.196356760490534	26.496727466878323	26.211955545236375	23.094960227394772
20-21	24.720349589861975	25.57524845407678	26.04259680324524	23.661805152816008
22-23	24.53988587645725	25.328996322769186	25.793325342787433	24.337792457986136
24-25	24.343775789415005	25.68047403853481	25.689509123816638	24.28624104823355
26-27	24.187206453217566	25.821922467082615	25.77974702577049	24.211124053929332
28-29	24.320672349019215	25.96925583253193	25.648888303192614	24.061183515256243
30-31	24.616555526306097	25.659851846193927	25.55104159136447	24.172551036135506
32-33	24.34606242808311	25.656912708470546	25.479359286867442	24.517665576578906
34-35	24.568580813769817	25.74437806912902	25.62656988778558	24.060471229315585
36-37	24.529319248738254	25.757891388419996	25.627514669937906	24.085274692903845
38-39	24.55988691646554	25.759490196733577	25.56394309956213	24.116679787238755
40-41	24.591324585852227	25.60072801155026	25.642461157743625	24.16548624485389
42-43	24.448362186188547	25.5944977039101	25.507908451001978	24.449231658899375
44-45	24.45581728547193	25.59032438283281	25.517714973887717	24.43614335780754
46-47	24.54776308846633	25.613372283829435	25.375918602896736	24.4629460248075
48-49	24.30526122328036	25.719630336469102	25.546463506075074	24.428644934175463
50-51	24.448879961785146	25.668921893108198	25.548238921937433	24.33395922316922
52-53	24.666605523605085	25.5614535501389	25.444969198898608	24.326971727357403
54-55	24.253599481627592	25.48434185644078	25.666252930207072	24.595805731724553
56-57	24.295103889386503	25.59965821979018	25.53054524240516	24.574692648418157
58-59	24.40016202990188	25.574520797929072	25.606896090824034	24.418421081345013
60-61	24.423221571431398	25.556201118232757	25.684182782434238	24.336394527901607
62-63	24.376701074484878	25.58748503634159	25.64944536638521	24.386368522788324
64-65	24.22346796660944	25.41029216482184	25.623881639541796	24.74235822902692
66-67	24.2478488528352	25.64198458077358	25.46894121402874	24.641225352362476
68-69	24.238638750883283	25.57247886263372	25.50773331587233	24.681149070610672
70-71	24.18155557522495	25.349150256506547	25.62067319474595	24.848620973522547
72-73	24.193268409665706	25.57431731931368	25.4169645025141	24.81544976850652
74-75	24.10943291601829	25.65443981670467	25.44288356272531	24.793243704551724
76-77	24.171806900586752	25.35597736098471	25.534508572676458	24.937707165752084
78-79	24.02970786154982	25.38726392628509	25.598159174310048	24.98486903785504
80-81	24.16959117691958	25.545451366709283	25.420596455109802	24.864361001261344
82-83	24.1402128693854	25.574590986973206	25.48741148842668	24.797784655214716
84-85	24.134084195003034	25.545701405614686	25.46195591006849	24.858258489313794
86-87	23.994429906529298	25.683167323574178	25.40727299156399	24.915129778332528
88-89	23.952936808895906	25.500999726182023	25.47207962531035	25.073983839611717
90-91	23.94450113492111	25.410039738057506	25.50801251055358	25.137446616467805
92-93	23.971490259706478	25.425630965361613	25.56697211842366	25.03590665650825
94-95	23.856727548343727	25.317349087320373	25.610741751549092	25.215181612786807
96-97	23.775796604887283	25.314714786288544	25.614090002487405	25.295398606336768
98-99	23.60768389551034	25.32032284157868	25.536260864451034	25.53573239845995
100-101	23.6627658997761	25.31226421430795	25.53316333749629	25.49180654841966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	15.5
2	18.5
3	28.5
4	27.0
5	30.0
6	46.0
7	124.5
8	216.0
9	236.5
10	210.5
11	186.5
12	181.0
13	197.5
14	251.5
15	282.0
16	317.0
17	367.0
18	412.0
19	469.0
20	518.0
21	587.0
22	730.5
23	918.0
24	1138.0
25	1495.5
26	1931.5
27	2641.0
28	3846.0
29	5680.5
30	8520.0
31	12538.0
32	17954.0
33	23317.5
34	28744.5
35	35241.0
36	43537.0
37	52259.0
38	60510.0
39	68758.5
40	76303.5
41	83453.0
42	90881.5
43	98918.0
44	106477.5
45	114184.5
46	120181.0
47	125294.0
48	130845.0
49	132745.5
50	131577.0
51	130516.0
52	129878.5
53	128914.5
54	127289.5
55	122405.0
56	114228.5
57	103593.0
58	92862.0
59	82162.0
60	68475.5
61	56183.0
62	46179.0
63	35422.0
64	25832.0
65	19307.5
66	15040.5
67	11853.5
68	9134.5
69	7116.0
70	5526.0
71	4166.0
72	3054.0
73	2355.5
74	1897.5
75	1446.0
76	1021.5
77	666.0
78	444.5
79	274.0
80	157.5
81	105.5
82	71.5
83	37.5
84	23.0
85	16.5
86	13.0
87	8.0
88	8.0
89	7.5
90	3.5
91	3.0
92	1.5
93	1.5
94	1.5
95	1.5
96	1.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14605911439217553
2	4.432232696307848E-4
3	0.005761902505200202
4	0.0047049854776191
5	0.02649111388485537
6	0.0012955757112284479
7	1.3637639065562608E-4
8	0.0019433635668426718
9	0.0
10-11	0.10015141188772542
12-13	3.2389392780711197E-4
14-15	0.005284585137905511
16-17	2.3865868364734565E-4
18-19	3.409409766390652E-4
20-21	7.500701486059436E-4
22-23	3.2389392780711197E-4
24-25	0.0016535637366994662
26-27	0.002471822080633223
28-29	0.0060517023353434075
30-31	0.014694556093143712
32-33	0.014336568067672693
34-35	0.011097628789601573
36-37	6.818819532781304E-4
38-39	2.0456458598343914E-4
40-41	0.004722032526451054
42-43	0.008421242122984911
44-45	0.008301912781161238
46-47	0.009427018004070153
48-49	0.011523805010400404
50-51	0.005727808407536296
52-53	0.0016024225902036065
54-55	0.001108058174076962
56-57	0.006136937579503175
58-59	0.009103124076263042
60-61	0.007398419193067715
62-63	0.018325577494349755
64-65	0.03520215583798348
66-67	0.0386968008485339
68-69	0.027650313205428188
70-71	0.04304379830068199
72-73	0.027104807642805682
74-75	0.025297820466618638
76-77	0.2372949197407894
78-79	0.06760859566752664
80-81	0.05647687278026116
82-83	0.020575787940167588
84-85	0.03276442785501417
86-87	0.0820985871746869
88-89	0.2645020096765868
90-91	0.3166489320535318
92-93	0.05112409944702783
94-95	0.004415185647475894
96-97	0.009699770785381406
98-99	0.0014149050530521208
100-101	0.0015683284925397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2933059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.81353820540867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09883990708676	48.893791626002105
2	11.729331559785571	14.500629689894224
3	3.7137614666458063	6.886822089128552
4	1.7988114235136399	4.447635946067438
5	0.9617394897857026	2.972426034775938
6	0.6037101137504264	2.2390474908782156
7	0.3871141329070126	1.675022597101089
8	0.30568927218246406	1.5116588404027451
9	0.20684855825623505	1.150743713265494
>10	1.1327414605028583	11.987882572806496
>50	0.0466549066410671	1.939402543434416
>100	0.014094294043958257	1.5044975391838735
>500	6.634148985621754E-4	0.2904393170594255
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.409409766390652E-5
2	0.0	0.0	0.0	0.0	3.409409766390652E-5
3	0.0	0.0	0.0	0.0	3.409409766390652E-5
4	0.0	0.0	0.0	0.0	3.409409766390652E-5
5	0.0	0.0	0.0	0.0	3.409409766390652E-5
6	0.0	0.0	0.0	0.0	3.409409766390652E-5
7	0.0	0.0	0.0	0.0	6.818819532781304E-5
8	0.0	0.0	0.0	0.0	6.818819532781304E-5
9	0.0	0.0	0.0	0.0	6.818819532781304E-5
10-11	0.0	0.0	0.0	0.0	6.818819532781304E-5
12-13	0.0	0.0	0.0	3.409409766390652E-5	1.704704883195326E-4
14-15	0.0	0.0	0.0	3.409409766390652E-5	2.3865868364734565E-4
16-17	0.0	0.0	0.0	3.409409766390652E-5	3.750350743029717E-4
18-19	0.0	0.0	0.0	3.409409766390652E-5	4.091291719668783E-4
20-21	0.0	0.0	0.0	3.409409766390652E-5	4.943644161266446E-4
22-23	0.0	0.0	0.0	5.114114649585978E-5	5.455055626225043E-4
24-25	0.0	0.0	0.0	2.3865868364734565E-4	5.795996602864109E-4
26-27	0.0	0.0	0.0	4.091291719668783E-4	6.818819532781304E-4
28-29	0.0	0.0	0.0	0.0017217519320272792	6.818819532781304E-4
30-31	0.0	0.0	0.0	0.0059494200423516885	6.818819532781304E-4
32-33	0.0	0.0	0.0	0.01735389571092842	7.500701486059434E-4
34-35	0.0	0.0	0.0	0.03315650997814909	8.523524415976631E-4
36-37	0.0	0.0	0.0	0.05659620212208483	8.523524415976631E-4
38-39	1.704704883195326E-5	0.0	0.0	0.09662267277951109	8.523524415976631E-4
40-41	3.409409766390652E-5	3.409409766390652E-5	0.0	0.14510447965758616	8.693994904296163E-4
42-43	3.409409766390652E-5	3.409409766390652E-5	0.0	0.19725140203453118	9.034935880935229E-4
44-45	3.409409766390652E-5	3.409409766390652E-5	0.0	0.26063232959173344	9.205406369254761E-4
46-47	3.409409766390652E-5	3.409409766390652E-5	0.0	0.3367985437729006	9.205406369254761E-4
48-49	3.409409766390652E-5	3.409409766390652E-5	0.0	0.41284542861224405	9.546347345893826E-4
50-51	3.409409766390652E-5	3.409409766390652E-5	0.0	0.4955577095448813	9.887288322532892E-4
52-53	3.409409766390652E-5	3.409409766390652E-5	0.0	0.586520762112184	0.001039869978749149
54-55	3.409409766390652E-5	3.409409766390652E-5	0.0	0.6918374297959912	0.0010910111252450086
56-57	3.409409766390652E-5	5.114114649585978E-5	0.0	0.8154285338276523	0.0011421522717408685
58-59	3.409409766390652E-5	1.0228229299171957E-4	0.0	0.9451395283899846	0.0011762463694047751
60-61	3.409409766390652E-5	1.0228229299171957E-4	0.0	1.0768279806168235	0.0011932934182367282
62-63	3.409409766390652E-5	1.0228229299171957E-4	0.0	1.21966520277976	0.0012273875159006349
64-65	3.409409766390652E-5	1.0228229299171957E-4	0.0	1.379072156407355	0.0012614816135645412
66-67	3.409409766390652E-5	1.0228229299171957E-4	0.0	1.5748745592911702	0.0012955757112284479
68-69	3.409409766390652E-5	1.0228229299171957E-4	0.0	1.7827292256991762	0.0013126227600604012
70-71	3.409409766390652E-5	1.0228229299171957E-4	0.0	1.997641370323611	0.0013978580042201675
72-73	3.409409766390652E-5	1.0228229299171957E-4	0.0	2.2239750376654546	0.0015853755413716532
74-75	3.409409766390652E-5	1.0228229299171957E-4	0.0	2.4775328419919274	0.0016194696390355598
76-77	3.409409766390652E-5	1.0228229299171957E-4	0.0	2.772617257273038	0.001704704883195326
78-79	3.409409766390652E-5	1.0228229299171957E-4	0.0	3.097397631619412	0.0017387989808592325
80-81	6.818819532781304E-5	1.0228229299171957E-4	0.0	3.429150248938054	0.0017899401273550925
82-83	6.818819532781304E-5	1.0228229299171957E-4	0.0	3.7724266712670973	0.0019774576645065784
84-85	6.818819532781304E-5	1.0228229299171957E-4	0.0	4.142296489773987	0.0019774576645065784
86-87	6.818819532781304E-5	1.0228229299171957E-4	0.0	4.558636563396782	0.0019774576645065784
88-89	6.818819532781304E-5	1.0228229299171957E-4	0.0	5.007860394216413	0.0019774576645065784
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2295	0.0	47.210835	1
GTATCAA	4040	0.0	41.28718	1
TCAACGC	5035	0.0	32.04508	4
CAACGCA	5120	0.0	31.47298	5
TATCAAC	5300	0.0	30.621897	2
ATCAACG	5365	0.0	30.162443	3
AACGCAG	5520	0.0	29.019112	6
GTACATG	8305	0.0	23.517529	1
ACGCAGA	6690	0.0	23.446669	7
CGCAGAG	6985	0.0	22.04814	8
TACATGG	8575	0.0	21.583025	2
ACATGGG	8920	0.0	20.26945	3
GGACCGT	470	0.0	19.215384	6
CATGGGG	7920	0.0	18.394798	4
GCAGAGT	8120	0.0	17.795528	9
AGAGTAC	6710	0.0	17.780523	10-11
TATACCG	265	7.89056E-6	16.143763	5
CTATACT	1035	0.0	16.047579	4
TAAGTAC	1785	0.0	15.685402	3
CAGAGTA	7455	0.0	15.684864	10-11
>>END_MODULE