FastQCFastQC Report
Mon 15 Aug 2016
SRR2921410_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921410_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515499
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.054.4492951
GTATCAA44200.045.6378361
TCAACGC54150.036.7238354
CAACGCA55550.035.710735
ATCAACG57250.034.4865953
TATCAAC57350.034.42922
AACGCAG58950.033.5592276
GTACATG92950.030.535861
TACATGG95750.028.9990712
ACATGGG98850.027.1751673
ACGCAGA71800.026.8300677
GTATACG1109.530898E-525.88911
CGCAGAG74300.025.675958
CATGGGG82250.024.2351574
ATGGGGG54900.022.5618485
GCAGAGT81700.022.0152519
GAGTACA67050.020.3869421
GTACACT27400.019.2267064
AGTACAC26800.018.9487863
GTATAGG6850.018.708181
Produced by FastQC (version 0.11.5)