##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921410_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2515499
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.602687975626306	31.0	31.0	34.0	27.0	34.0
2	30.65572039583399	33.0	31.0	34.0	27.0	34.0
3	30.705072432944714	33.0	31.0	34.0	27.0	34.0
4	34.17776035689142	37.0	35.0	37.0	30.0	37.0
5	34.17385337859407	37.0	35.0	37.0	30.0	37.0
6	34.224981206512105	37.0	35.0	37.0	30.0	37.0
7	34.151730928932984	37.0	35.0	37.0	30.0	37.0
8	34.11847629436545	37.0	35.0	37.0	30.0	37.0
9	35.695932298124546	39.0	37.0	39.0	30.0	39.0
10-11	35.58697717629782	39.0	37.0	39.0	30.0	39.0
12-13	35.47390696637129	39.0	37.0	39.0	30.0	39.0
14-15	36.61719185736111	40.0	37.0	41.0	30.0	41.0
16-17	36.56384200510515	40.0	37.0	41.0	30.0	41.0
18-19	36.521508257407376	40.0	37.0	41.0	29.5	41.0
20-21	36.46597613435744	40.0	37.0	41.0	29.0	41.0
22-23	36.35551256430632	40.0	37.0	41.0	28.5	41.0
24-25	36.25515017099987	40.0	36.0	41.0	28.0	41.0
26-27	36.10635186100252	40.0	36.0	41.0	27.0	41.0
28-29	35.96373622092476	40.0	36.0	41.0	27.0	41.0
30-31	35.81305876090589	40.0	36.0	41.0	27.0	41.0
32-33	35.62591935039529	40.0	35.5	41.0	26.5	41.0
34-35	35.464269117181125	39.0	35.0	41.0	25.5	41.0
36-37	35.292502402107885	39.0	35.0	41.0	25.0	41.0
38-39	35.5247127905835	39.5	35.0	41.0	26.0	41.0
40-41	35.64229761172634	40.0	35.0	41.0	26.0	41.0
42-43	35.47884515159815	40.0	35.0	41.0	25.0	41.0
44-45	35.353183404167524	40.0	35.0	41.0	24.5	41.0
46-47	35.186677275562424	40.0	35.0	41.0	24.0	41.0
48-49	35.041648396600436	40.0	35.0	41.0	23.0	41.0
50-51	33.3186196853984	37.0	32.5	39.5	20.0	40.0
52-53	33.480214462418786	37.5	33.5	39.5	21.0	40.0
54-55	34.35351117213722	39.0	34.0	40.5	21.5	41.0
56-57	34.37605779211202	39.0	34.0	41.0	22.0	41.0
58-59	34.19978441653127	38.5	34.0	41.0	20.5	41.0
60-61	33.95018006367722	38.0	34.0	40.5	20.0	41.0
62-63	33.65803981635453	37.5	34.0	40.0	18.5	41.0
64-65	33.371765601974005	37.0	34.0	40.0	18.0	41.0
66-67	33.03195648259053	36.5	33.0	39.5	14.0	41.0
68-69	32.66195534166383	36.0	33.0	39.0	12.0	41.0
70-71	32.27316170668325	35.5	33.0	39.0	8.0	40.5
72-73	31.905976110505314	35.0	32.5	37.5	6.5	40.0
74-75	31.49984893653307	35.0	32.0	37.0	2.0	39.0
76-77	31.122221078203566	35.0	32.0	37.0	2.0	39.0
78-79	30.770585279501205	35.0	32.0	36.0	2.0	38.5
80-81	30.416648148140787	35.0	31.5	36.0	2.0	37.0
82-83	30.106856134707268	35.0	31.0	35.0	2.0	37.0
84-85	29.863203086147124	35.0	31.0	35.0	2.0	36.5
86-87	29.636043385427705	35.0	31.0	35.0	2.0	36.0
88-89	29.431350002524347	35.0	31.0	35.0	2.0	36.0
90-91	29.17121970630877	35.0	31.0	35.0	2.0	35.5
92-93	28.974769618274543	34.0	30.5	35.0	2.0	35.0
94-95	28.757242002481416	34.0	30.0	35.0	2.0	35.0
96-97	28.494766644709458	34.0	30.0	35.0	2.0	35.0
98-99	28.26484864434452	34.0	30.0	35.0	2.0	35.0
100-101	27.21189056326399	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75153.0
3	13484.0
4	9532.0
5	7201.0
6	6651.0
7	6876.0
8	7347.0
9	7703.0
10	8301.0
11	9126.0
12	9312.0
13	9620.0
14	10442.0
15	11571.0
16	11932.0
17	12834.0
18	11811.0
19	10317.0
20	10516.0
21	11233.0
22	12183.0
23	13959.0
24	15865.0
25	18884.0
26	22198.0
27	26415.0
28	31403.0
29	36769.0
30	44105.0
31	53583.0
32	67233.0
33	87275.0
34	124742.0
35	179172.0
36	291416.0
37	537728.0
38	583431.0
39	108159.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32943356978509	17.956253442868764	12.766405953254543	24.94790703409161
2	16.147379432828636	19.750960102304845	39.24011208983097	24.86154837503555
3	19.529463329035217	23.60307810035326	29.264106833055052	27.60335173755647
4	13.54424458800591	15.304688923201512	36.29208102339649	34.85898546539609
5	14.302276790419544	37.508953837770974	33.937680095931846	14.251089275877634
6	33.80758368498209	34.531702462409406	17.160048460134302	14.500665392474207
7	29.32990291208094	32.01181801591401	20.528216487094372	18.13006258491068
8	27.16989279463186	34.57287635000751	19.890647502610353	18.366583352750272
9	27.737218731374174	15.361498598999837	19.00875113171469	37.8925315379113
10-11	26.08916764879433	25.107218916653544	27.49516093374495	21.308452500807174
12-13	26.416632386661753	22.523981257568856	27.465809298725542	23.59357705704385
14-15	23.670758557495137	24.069759672669942	24.79738737291223	27.462094396922694
16-17	23.747686903284674	27.208220756164664	25.689763601366572	23.35432873918409
18-19	23.811750522283432	26.72918162739302	27.006705586424683	22.452362263898863
20-21	24.25056987010043	25.758963586965706	27.060404739490085	22.930061803443774
22-23	24.107357875560364	25.543173809056483	26.691277699592277	23.658190615790872
24-25	23.810970680940393	25.793009344166425	26.67697770870166	23.71904226619152
26-27	23.57020590673305	26.037591265620858	26.789368858751512	23.602833968894583
28-29	23.58368880279159	26.558876716511914	26.391062527634368	23.466371953062126
30-31	24.27566670311211	25.97377916049579	26.37796574791019	23.372588388481915
32-33	23.86228957848706	25.981980312883536	26.352003320545997	23.803726788083406
34-35	24.087212385827	26.14228656895385	26.55882369311825	23.211677352100896
36-37	23.992292469534423	26.19492959941355	26.532680676551664	23.280097254500365
38-39	24.00555396530064	26.23284512507355	26.424152791692507	23.337448117933306
40-41	24.101174104463833	25.974767895368007	26.432864827215802	23.491193172952364
42-43	23.78262304531618	25.99731225683782	26.27389445299925	23.946170244846748
44-45	23.627669185748623	26.05524350517135	26.52338213984101	23.793705169239015
46-47	23.81714344433363	25.950674381776878	26.248753357570504	23.983428816318987
48-49	23.5854345083584	26.173337355959607	26.345294672310292	23.895933463371698
50-51	23.76560382309846	26.110672789975318	26.424770485450505	23.69895290147572
52-53	23.964085963474584	26.00445888476103	26.33414551816363	23.697309633600756
54-55	23.26597394176507	25.78895402628924	26.73287102993873	24.21220100200696
56-57	23.501444721766585	25.925366796812447	26.434942908196636	24.13824557322433
58-59	23.66883656162134	25.995422547974222	26.544900016609795	23.790840873794643
60-61	23.93847631976444	25.888705920218225	26.59113034804943	23.581687411967902
62-63	23.79598468375491	25.812644864425422	26.731492107067133	23.659878344752535
64-65	23.420464528428223	25.52944613764972	26.828756301721015	24.221333032201038
66-67	23.4638243264557	25.947088278074897	26.483820757798078	24.105266637671324
68-69	23.445746346440995	25.80418524827633	26.56891050597605	24.181157899306626
70-71	23.443773221247465	25.597948366697594	26.646861821382068	24.311416590672874
72-73	23.28825333370769	25.957708552495728	26.430372517827198	24.32366559596938
74-75	23.205722333720363	25.893074223114382	26.626267147775778	24.274936295389473
76-77	23.20591659586263	25.459625569261483	26.6784439983696	24.65601383650629
78-79	23.15880736483029	25.42919119555304	26.846600160413292	24.565401279203375
80-81	23.32658356336066	25.68351878269897	26.480922504859155	24.50897514908121
82-83	23.33987852944621	25.75064595468637	26.582399756517113	24.327075759350308
84-85	23.298667392198908	25.73698154951857	26.527073413532264	24.437277644750253
86-87	22.951985194953693	25.930146157998852	26.576452565882658	24.5414160811648
88-89	22.976969981892324	25.5119452905097	26.75824755924836	24.75283716834961
90-91	23.19977306800032	25.24983037868879	26.884908105687522	24.665488447623368
92-93	23.341390013368922	25.237900487484588	26.823306110081752	24.597403389064734
94-95	23.19245249487927	25.161949841222913	26.83126594719065	24.814331716707166
96-97	22.943919070496538	25.033759808673828	26.977862570782037	25.0444585500476
98-99	22.736002693331713	24.863336324817396	27.080075819678257	25.32058516217263
100-101	22.933189835079943	24.880457414628733	27.108219983459982	25.07813276683134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	128.0
2	166.5
3	221.5
4	208.5
5	237.5
6	356.5
7	617.5
8	934.5
9	1034.5
10	870.5
11	723.0
12	717.0
13	794.5
14	896.5
15	1016.5
16	1143.0
17	1236.5
18	1395.0
19	1533.5
20	1633.0
21	1757.5
22	1923.5
23	2178.5
24	2457.0
25	2925.5
26	3638.5
27	4631.0
28	6080.5
29	8343.0
30	11624.0
31	16207.0
32	22429.0
33	27734.5
34	32302.5
35	38148.5
36	45645.5
37	52489.5
38	58354.5
39	64212.5
40	68624.0
41	72355.0
42	76814.0
43	83214.0
44	89866.5
45	96845.5
46	101560.5
47	105245.0
48	110989.0
49	114119.0
50	113625.5
51	111395.0
52	108537.0
53	105715.5
54	103288.5
55	98133.5
56	89654.5
57	80686.0
58	72598.5
59	63475.5
60	51716.5
61	41593.0
62	33802.5
63	25986.0
64	18913.5
65	13878.5
66	10477.0
67	7899.5
68	6073.5
69	4647.5
70	3514.0
71	2638.0
72	1927.5
73	1544.0
74	1261.5
75	988.5
76	766.5
77	533.0
78	342.5
79	266.5
80	214.0
81	140.0
82	102.0
83	80.5
84	68.5
85	65.5
86	48.0
87	38.5
88	36.0
89	30.0
90	27.5
91	25.0
92	22.0
93	16.0
94	12.5
95	10.5
96	9.5
97	8.5
98	7.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04945340864774743
2	0.05740411743355891
3	0.04853907713737911
4	0.05410457328744714
5	0.0482608023298757
6	0.017571066416643376
7	0.014152261638744442
8	0.020393568035606453
9	0.020274307403819282
10-11	0.021904202704910634
12-13	0.026654751204432996
14-15	0.04353013060231787
16-17	0.029517006367325134
18-19	0.02309680902278236
20-21	0.026694504748362054
22-23	0.046889305064323226
24-25	0.041443069546042356
26-27	0.031504683563778
28-29	0.02047307512346457
30-31	0.012880148233014602
32-33	0.009938385982264354
34-35	0.009421589911186608
36-37	0.0014708811253751244
38-39	0.007115884363301277
40-41	0.0036772028134378107
42-43	0.2072948548180699
44-45	0.18276691821384147
46-47	0.189028101382668
48-49	0.18690128678246343
50-51	0.18509250053369133
52-53	0.19008157029678802
54-55	0.18835229113587404
56-57	0.198012402310635
58-59	0.19606447865811116
60-61	0.20079515038566897
62-63	0.19725708497598288
64-65	0.1977540042750961
66-67	0.18886908720695178
68-69	0.18795475569658346
70-71	0.17209309166888953
72-73	0.17962638824344593
74-75	0.17871205673307763
76-77	0.17732068269556062
78-79	0.2030809791615898
80-81	0.21029624738471372
82-83	0.20914339461077106
84-85	0.20504877958607814
86-87	0.2203936475426943
88-89	0.2192010412248226
90-91	0.21848547743409955
92-93	0.22156637708860152
94-95	0.23249860166909228
96-97	0.23287626033641837
98-99	0.22248070859896982
100-101	0.23170353079051115
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2515499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.05245904080642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98154299816932	48.83069877916229
2	11.226358674311648	13.707936062816273
3	3.61231389753123	6.6162193881468365
4	1.639946186962354	4.00490989634583
5	0.9046850855679874	2.7616624565734
6	0.5449845448998206	1.9963587963221292
7	0.39995702102614816	1.7092851750997304
8	0.269993958467868	1.3187036072499758
9	0.19784308497110248	1.0870926157534515
>10	1.1391880770303964	12.276748481783489
>50	0.05784750413852613	2.4143547066068654
>100	0.023836921940517064	2.5231041012031263
>500	0.0012408197686672826	0.49045445356309697
>1k	2.6122521445310227E-4	0.26247147937359677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.975354392905742E-5
2	0.0	0.0	0.0	0.0	3.975354392905742E-5
3	0.0	0.0	0.0	0.0	3.975354392905742E-5
4	0.0	0.0	0.0	0.0	3.975354392905742E-5
5	0.0	0.0	0.0	3.975354392905742E-5	3.975354392905742E-5
6	0.0	0.0	0.0	3.975354392905742E-5	3.975354392905742E-5
7	0.0	0.0	0.0	3.975354392905742E-5	3.975354392905742E-5
8	0.0	0.0	0.0	3.975354392905742E-5	7.950708785811484E-5
9	0.0	0.0	0.0	3.975354392905742E-5	1.5901417571622967E-4
10-11	0.0	0.0	0.0	3.975354392905742E-5	1.5901417571622967E-4
12-13	0.0	0.0	0.0	3.975354392905742E-5	1.5901417571622967E-4
14-15	0.0	0.0	0.0	3.975354392905742E-5	1.5901417571622967E-4
16-17	0.0	0.0	0.0	5.963031589358613E-5	1.7889094768075837E-4
18-19	0.0	0.0	0.0	9.938385982264354E-5	1.9876771964528708E-4
20-21	0.0	0.0	0.0	1.7889094768075837E-4	1.9876771964528708E-4
22-23	0.0	0.0	0.0	3.3790512339698805E-4	1.9876771964528708E-4
24-25	1.987677196452871E-5	0.0	0.0	9.540850542973779E-4	2.1864449160981578E-4
26-27	3.975354392905742E-5	0.0	0.0	0.0016696488450204114	2.3852126357434448E-4
28-29	3.975354392905742E-5	0.0	0.0	0.006122045765074842	2.3852126357434448E-4
30-31	3.975354392905742E-5	0.0	0.0	0.021943956248839692	2.3852126357434448E-4
32-33	7.950708785811484E-5	0.0	0.0	0.05239517089849767	2.3852126357434448E-4
34-35	1.1926063178717224E-4	3.975354392905742E-5	0.0	0.0940966384800789	2.3852126357434448E-4
36-37	1.1926063178717224E-4	7.950708785811484E-5	0.0	0.15589352251779864	2.3852126357434448E-4
38-39	1.3913740375170097E-4	1.3913740375170097E-4	0.0	0.2591334761015608	2.3852126357434448E-4
40-41	1.5901417571622967E-4	1.5901417571622967E-4	0.0	0.37537283855012465	2.3852126357434448E-4
42-43	1.5901417571622967E-4	1.5901417571622967E-4	0.0	0.49841005701055735	2.3852126357434448E-4
44-45	1.5901417571622967E-4	1.5901417571622967E-4	0.0	0.6485989459745363	2.3852126357434448E-4
46-47	1.5901417571622967E-4	1.5901417571622967E-4	0.0	0.8193602939218023	2.3852126357434448E-4
48-49	1.5901417571622967E-4	1.9876771964528708E-4	0.0	0.9916123997664081	2.3852126357434448E-4
50-51	1.5901417571622967E-4	2.1864449160981578E-4	0.0	1.1762079810009864	2.3852126357434448E-4
52-53	1.5901417571622967E-4	2.3852126357434448E-4	0.0	1.3699667541112122	2.3852126357434448E-4
54-55	1.5901417571622967E-4	2.3852126357434448E-4	0.0	1.5945345237664574	2.3852126357434448E-4
56-57	1.5901417571622967E-4	2.3852126357434448E-4	0.0	1.847009281259901	2.3852126357434448E-4
58-59	1.5901417571622967E-4	2.3852126357434448E-4	0.0	2.111211334212417	2.3852126357434448E-4
60-61	1.5901417571622967E-4	2.782748075034019E-4	0.0	2.386842531044536	2.3852126357434448E-4
62-63	1.5901417571622967E-4	2.782748075034019E-4	0.0	2.6765464824275425	2.3852126357434448E-4
64-65	1.5901417571622967E-4	2.782748075034019E-4	0.0	2.9917125786971095	2.3852126357434448E-4
66-67	1.5901417571622967E-4	2.782748075034019E-4	0.0	3.3573259222126506	2.782748075034019E-4
68-69	1.5901417571622967E-4	2.782748075034019E-4	0.0	3.7475268326483135	2.782748075034019E-4
70-71	1.5901417571622967E-4	2.782748075034019E-4	0.0	4.155259055956691	2.782748075034019E-4
72-73	1.7889094768075837E-4	2.782748075034019E-4	0.0	4.574181901881097	3.1802835143245934E-4
74-75	1.9876771964528708E-4	2.782748075034019E-4	0.0	5.016777983215259	3.1802835143245934E-4
76-77	1.9876771964528708E-4	3.1802835143245934E-4	0.0	5.511928249623633	3.1802835143245934E-4
78-79	1.9876771964528708E-4	3.1802835143245934E-4	0.0	6.059235165666932	3.1802835143245934E-4
80-81	1.9876771964528708E-4	3.5778189536151675E-4	0.0	6.625603906024212	3.1802835143245934E-4
82-83	1.9876771964528708E-4	3.5778189536151675E-4	0.0	7.207019362758642	3.5778189536151675E-4
84-85	1.9876771964528708E-4	3.5778189536151675E-4	0.0	7.819522090845593	3.5778189536151675E-4
86-87	1.9876771964528708E-4	3.5778189536151675E-4	0.0	8.482273298458875	3.5778189536151675E-4
88-89	1.9876771964528708E-4	3.5778189536151675E-4	0.0	9.198890558096029	3.5778189536151675E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2310	0.0	54.449295	1
GTATCAA	4420	0.0	45.637836	1
TCAACGC	5415	0.0	36.723835	4
CAACGCA	5555	0.0	35.71073	5
ATCAACG	5725	0.0	34.486595	3
TATCAAC	5735	0.0	34.4292	2
AACGCAG	5895	0.0	33.559227	6
GTACATG	9295	0.0	30.53586	1
TACATGG	9575	0.0	28.999071	2
ACATGGG	9885	0.0	27.175167	3
ACGCAGA	7180	0.0	26.830067	7
GTATACG	110	9.530898E-5	25.8891	1
CGCAGAG	7430	0.0	25.67595	8
CATGGGG	8225	0.0	24.235157	4
ATGGGGG	5490	0.0	22.561848	5
GCAGAGT	8170	0.0	22.015251	9
GAGTACA	6705	0.0	20.386942	1
GTACACT	2740	0.0	19.226706	4
AGTACAC	2680	0.0	18.948786	3
GTATAGG	685	0.0	18.70818	1
>>END_MODULE