FastQCFastQC Report
Mon 15 Aug 2016
SRR2921410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515499
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.057.0324971
GTATCAA45400.042.0834471
TCAACGC57900.032.5442354
CAACGCA60200.031.1946495
ATCAACG61100.030.7621083
TATCAAC61150.030.7369582
GTACATG86500.030.6041411
AACGCAG63800.029.506036
TACATGG90400.028.772282
ACATGGG93300.027.1657523
ACGCAGA76300.023.7371237
CATGGGG82300.023.7030454
CGCAGAG79800.022.5173118
ATGGGGG52150.021.8794525
TACCGTA2056.78554E-720.8697037
TCTATAC4950.019.1772773
ATAGGAC5200.019.1680563
GCAGAGT88300.019.0576869
GAGTACA65700.018.6636031
GTAAGTA20250.018.5167262
Produced by FastQC (version 0.11.5)