##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2515499
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35866044868235	33.0	31.0	34.0	28.0	34.0
2	31.480328157554425	34.0	31.0	34.0	28.0	34.0
3	31.556807217971464	34.0	31.0	34.0	28.0	34.0
4	35.05640908622902	37.0	35.0	37.0	32.0	37.0
5	34.7916202709681	37.0	35.0	37.0	32.0	37.0
6	34.74950019856895	37.0	35.0	37.0	32.0	37.0
7	34.61317217776672	37.0	35.0	37.0	32.0	37.0
8	34.572200187716234	37.0	35.0	37.0	32.0	37.0
9	36.180063279691225	39.0	37.0	39.0	32.0	39.0
10-11	36.02867522507462	39.0	37.0	39.0	31.0	39.0
12-13	35.95582228416708	39.0	37.0	39.0	31.0	39.0
14-15	37.17284502995231	40.0	38.0	41.0	31.0	41.0
16-17	37.15154050945756	40.0	38.0	41.0	31.0	41.0
18-19	37.121402353966346	40.0	38.0	41.0	31.0	41.0
20-21	37.082415457131965	40.0	38.0	41.0	31.0	41.0
22-23	36.976805397259156	40.0	37.0	41.0	31.0	41.0
24-25	36.90342790833946	40.0	37.0	41.0	30.0	41.0
26-27	36.75915633438932	40.0	37.0	41.0	30.0	41.0
28-29	36.60419741768929	40.0	37.0	41.0	30.0	41.0
30-31	36.455548978552564	40.0	36.5	41.0	30.0	41.0
32-33	36.39208681855966	40.0	36.5	41.0	30.0	41.0
34-35	36.51517949321387	40.0	37.0	41.0	30.0	41.0
36-37	36.48545636472127	40.0	37.0	41.0	30.0	41.0
38-39	36.39387771571366	40.0	36.5	41.0	30.0	41.0
40-41	36.26373216606328	40.0	36.0	41.0	29.0	41.0
42-43	36.15820181204604	40.0	36.0	41.0	29.0	41.0
44-45	36.01052832857417	40.0	36.0	41.0	28.0	41.0
46-47	35.84127264610322	40.0	35.5	41.0	27.5	41.0
48-49	35.67526641831303	40.0	35.0	41.0	27.0	41.0
50-51	35.519255821608354	39.5	35.0	41.0	26.5	41.0
52-53	35.35669662361225	39.0	35.0	41.0	26.0	41.0
54-55	35.15117855344009	39.0	35.0	41.0	26.0	41.0
56-57	34.892766405393125	39.0	35.0	41.0	25.0	41.0
58-59	34.666376134516454	38.5	35.0	40.5	25.0	41.0
60-61	34.39229194684633	38.0	34.0	40.0	24.0	41.0
62-63	34.087443087832675	37.5	34.0	40.0	23.0	41.0
64-65	33.77506749157921	37.0	34.0	40.0	23.0	41.0
66-67	33.44472985280456	36.5	34.0	39.0	22.0	41.0
68-69	33.08420536044737	36.0	33.5	39.0	22.0	41.0
70-71	32.70264130496574	35.5	33.0	39.0	20.5	40.0
72-73	32.30513269931731	35.0	33.0	37.5	19.5	40.0
74-75	31.89923490329354	35.0	33.0	37.0	18.0	39.0
76-77	29.154277143421645	33.0	28.5	34.5	10.5	36.5
78-79	30.80563776809293	34.5	31.5	36.0	12.0	37.5
80-81	31.00245378749902	35.0	32.0	36.0	13.0	37.0
82-83	30.83625038213094	35.0	32.0	35.0	10.5	37.0
84-85	30.60747151956729	35.0	32.0	35.0	7.0	36.5
86-87	30.36658909425128	35.0	32.0	35.0	3.5	36.0
88-89	30.091790336629032	35.0	31.5	35.0	2.0	36.0
90-91	29.90835695025122	34.0	31.0	35.0	2.0	35.0
92-93	29.7649293440387	34.0	31.0	35.0	2.0	35.0
94-95	29.666232624222868	34.0	31.0	35.0	2.0	35.0
96-97	29.47701966886093	34.0	31.0	35.0	2.0	35.0
98-99	29.219570947951084	34.0	31.0	35.0	2.0	35.0
100-101	28.142932873358326	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48119.0
3	16484.0
4	9231.0
5	6440.0
6	5525.0
7	5709.0
8	5879.0
9	6203.0
10	6963.0
11	7519.0
12	8159.0
13	8809.0
14	9645.0
15	10852.0
16	12152.0
17	13327.0
18	11854.0
19	10250.0
20	9652.0
21	9531.0
22	10020.0
23	11093.0
24	13110.0
25	15815.0
26	19004.0
27	22377.0
28	27336.0
29	32506.0
30	39674.0
31	49255.0
32	62179.0
33	83112.0
34	121570.0
35	177797.0
36	303055.0
37	593312.0
38	630502.0
39	81475.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.177742247305886	19.55798535854369	13.34177624402215	25.92249615012828
2	15.21063490612167	21.213123486875322	37.669002858290035	25.907238748712974
3	18.083121532014303	24.810511865445832	29.12954925578324	27.976817346756626
4	13.148590976765082	16.494904378835134	35.342481001989746	35.01402364241004
5	14.032081691406576	37.85758878333724	33.98269465530473	14.127634869951446
6	33.69571915105752	35.29534768803626	16.86280714573364	14.146126015172577
7	29.029304757391785	32.25646154873631	20.552725983265326	18.161507710606575
8	27.016189144610646	35.23634529156004	19.606543717210155	18.140921846619154
9	27.445897392882046	15.378982610997902	18.96435616327264	38.21076383284741
10-11	25.934851342406002	25.500988556933695	27.463100473476693	21.101059627183613
12-13	26.321059976597	22.726848989033748	27.58761266942279	23.364478364946464
14-15	23.47683951653877	24.324868547747798	24.82775839024363	27.3705335454698
16-17	23.626954313338516	27.522054817474185	25.62684022041798	23.224150648769317
18-19	23.664792942925633	26.909687575780467	27.082934140068136	22.342585341225764
20-21	23.994102911235377	25.90226869361038	27.188524966522277	22.91510342863197
22-23	23.91966591068557	25.630462394081484	26.818439313137176	23.63143238209577
24-25	23.692643645809234	25.92857841395086	26.750714979140977	23.628062961098934
26-27	23.42520181973189	26.19876835946841	26.897914488025982	23.47811533277372
28-29	23.473889938230844	26.75212387385686	26.51326881967517	23.260717368237128
30-31	24.196061576434982	26.09760257769218	26.404228350319947	23.302107495552892
32-33	23.729033584235527	26.13557544987353	26.414309277456955	23.721081688433987
34-35	23.94031163364142	26.283487933549537	26.663915391120458	23.112285041688583
36-37	23.812877325571804	26.353860003454603	26.6709362235928	23.162326447380792
38-39	23.96837360772874	26.313698223887634	26.482193954746062	23.23573421363756
40-41	24.071628371469508	26.128838994788843	26.414065195923307	23.385467437818342
42-43	23.714395557386148	26.13651543607938	26.298565246915253	23.85052375961922
44-45	23.58092751955698	26.159568413597146	26.50694545355514	23.752558613290738
46-47	23.73450357776419	26.09440065392894	26.256908834004307	23.914186934302563
48-49	23.46814673157854	26.33508998272507	26.371230178057058	23.825533107639338
50-51	23.65988615482721	26.21629390151494	26.427497449655036	23.696322494002814
52-53	23.923485015633357	26.129368245506278	26.260616866531105	23.68652987232926
54-55	23.199250558592645	25.934381737672847	26.72260312024754	24.143764583486966
56-57	23.404141972431244	26.042425549551307	26.375798970122418	24.17763350789503
58-59	23.58629529514526	26.106439391981638	26.44805512606485	23.859210186808248
60-61	23.842644865193233	26.11038268137768	26.4552912888462	23.59168116458289
62-63	23.65761621673526	26.025300279539245	26.552669419846232	23.764414083879267
64-65	23.255447262812943	25.703337801179455	26.64735173242871	24.39386320357889
66-67	23.315453600781684	26.13564735886206	26.279627722970723	24.269271317385535
68-69	23.266812549531714	26.00000874820016	26.34592053532623	24.3872581669419
70-71	23.301453712309485	25.74466316611247	26.38527810554875	24.568605016029295
72-73	23.1709705324549	26.13752740469964	26.134286656687284	24.557215406158175
74-75	22.99397047447099	26.04720434359647	26.276400671285984	24.682424510646555
76-77	22.944700496636987	25.561354003728514	26.4802131193692	25.013732380265296
78-79	22.807785232816872	25.60574872290493	26.55064890531725	25.035817138960955
80-81	23.00452359824338	25.830131786143145	26.19124801274706	24.974096602866418
82-83	22.98393221390315	25.901541569084323	26.243851815329805	24.870674401682724
84-85	22.881382892632875	25.89086206505742	26.18245090510846	25.045304137201246
86-87	22.548854226261568	26.07376254128708	26.189258953060634	25.18812427939071
88-89	22.531224447946098	25.742620033746892	26.330769400927828	25.39538611737917
90-91	22.647608557725246	25.389720728225747	26.4704427967614	25.492227917287607
92-93	22.674832180397	25.429887649610766	26.40000477282829	25.495275397163947
94-95	22.47773048867198	25.373378201332326	26.440414434646716	25.70847687534898
96-97	22.12516991332482	25.283062976169795	26.570686924933597	26.021080185571787
98-99	21.889012678557762	25.14568365522512	26.611117019003427	26.35418664721369
100-101	21.872398585068805	25.271998180848183	26.532007292508958	26.32359594157406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	128.5
2	128.0
3	180.0
4	180.0
5	186.5
6	292.0
7	558.0
8	836.0
9	903.5
10	735.5
11	588.0
12	570.0
13	623.5
14	695.0
15	702.5
16	791.0
17	890.5
18	947.5
19	1070.5
20	1233.0
21	1415.0
22	1630.0
23	1895.0
24	2240.5
25	2758.5
26	3538.0
27	4832.0
28	6526.5
29	8982.5
30	12608.5
31	17503.5
32	23827.5
33	28976.0
34	33197.0
35	38495.0
36	45669.5
37	52411.5
38	58271.0
39	64289.5
40	68667.0
41	72236.5
42	76486.0
43	82958.0
44	90092.5
45	97067.0
46	101589.0
47	105585.5
48	111758.5
49	114810.0
50	114387.0
51	112457.0
52	109897.5
53	106815.0
54	103484.0
55	98214.0
56	90311.5
57	81087.0
58	72106.5
59	62922.5
60	51495.5
61	41097.0
62	33074.0
63	25109.5
64	17987.5
65	13146.5
66	9702.5
67	7369.0
68	5659.0
69	4260.0
70	3219.5
71	2359.0
72	1729.5
73	1385.5
74	1083.0
75	833.5
76	649.5
77	417.5
78	233.0
79	139.0
80	84.5
81	54.5
82	32.0
83	16.5
84	10.5
85	7.5
86	5.5
87	4.5
88	2.5
89	2.5
90	2.5
91	2.0
92	2.0
93	1.5
94	1.5
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.148161458223597
2	3.5778189536151675E-4
3	0.006758102467939761
4	0.004134368568621971
5	0.026595120888539412
6	0.0015901417571622966
7	1.1926063178717224E-4
8	0.0019081701085947558
9	3.975354392905742E-5
10-11	0.0990857082431756
12-13	3.7765866732604545E-4
14-15	0.005446235518280866
16-17	2.1864449160981578E-4
18-19	3.5778189536151675E-4
20-21	8.14947650545677E-4
22-23	1.5901417571622967E-4
24-25	0.001629895301091354
26-27	0.0029218854787857198
28-29	0.0065990882922235304
30-31	0.015146100236970876
32-33	0.014927455745361059
34-35	0.01200557026657534
36-37	6.1617993090039E-4
38-39	1.9876771964528708E-4
40-41	0.005048700078990292
42-43	0.008865040296179802
44-45	0.00876565643635716
46-47	0.009580604086902837
48-49	0.011687541915142879
50-51	0.006082292221145785
52-53	0.0017491559328785262
54-55	0.001013715370190964
56-57	0.006022661905252199
58-59	0.009779371806548124
60-61	0.007751941066166196
62-63	0.018584781786834342
64-65	0.03436693872667013
66-67	0.03657326041473282
68-69	0.027668466574623962
70-71	0.04134368568621971
72-73	0.025740419694064678
74-75	0.024826088183696356
76-77	0.2353211032880554
78-79	0.06712385892421345
80-81	0.05366728430422751
82-83	0.021029624738471375
84-85	0.032915934373259535
86-87	0.07954684140204389
88-89	0.2594912579969223
90-91	0.31286039072168187
92-93	0.050347863386151215
94-95	0.004790302043451418
96-97	0.009779371806548124
98-99	0.0012522366337653086
100-101	0.001629895301091354
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2515499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.57575636571367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35622962513226	48.07063631873641
2	11.74844869611893	14.233423317823735
3	3.6587559059424484	6.648957190799573
4	1.6664676237013856	4.037901470587398
5	0.8999238800734739	2.7256784853509246
6	0.5622097185741123	2.0433767363269126
7	0.3878279861346412	1.6445081519928189
8	0.2733963948114818	1.3248954722691832
9	0.20842461144363386	1.136293063508529
>10	1.1536139322147572	12.43679768803637
>50	0.05992503405810786	2.483408308697141
>100	0.02345830884149753	2.5202551248791174
>500	0.0011205405102233848	0.4594114931779241
>1k	1.977424429793927E-4	0.2344571778140066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.975354392905742E-5	0.0	0.0	0.0	3.975354392905742E-5
7	3.975354392905742E-5	0.0	0.0	0.0	3.975354392905742E-5
8	3.975354392905742E-5	0.0	0.0	0.0	3.975354392905742E-5
9	3.975354392905742E-5	0.0	0.0	0.0	3.975354392905742E-5
10-11	3.975354392905742E-5	0.0	1.987677196452871E-5	3.975354392905742E-5	3.975354392905742E-5
12-13	3.975354392905742E-5	0.0	3.975354392905742E-5	3.975354392905742E-5	3.975354392905742E-5
14-15	3.975354392905742E-5	1.987677196452871E-5	3.975354392905742E-5	3.975354392905742E-5	5.963031589358613E-5
16-17	3.975354392905742E-5	3.975354392905742E-5	3.975354392905742E-5	7.950708785811484E-5	7.950708785811484E-5
18-19	3.975354392905742E-5	5.963031589358613E-5	3.975354392905742E-5	7.950708785811484E-5	1.1926063178717224E-4
20-21	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	1.3913740375170097E-4	1.1926063178717224E-4
22-23	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	3.1802835143245934E-4	1.1926063178717224E-4
24-25	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	9.739618262619066E-4	1.1926063178717224E-4
26-27	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	0.001709402388949469	1.1926063178717224E-4
28-29	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	0.0063605670286491865	1.1926063178717224E-4
30-31	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	0.02244087554795291	1.3913740375170097E-4
32-33	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	0.05336913272475958	1.5901417571622967E-4
34-35	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	0.09586567118492195	1.9876771964528708E-4
36-37	3.975354392905742E-5	7.950708785811484E-5	3.975354392905742E-5	0.15853713318908097	1.9876771964528708E-4
38-39	3.975354392905742E-5	1.1926063178717225E-4	3.975354392905742E-5	0.2625721576514242	1.9876771964528708E-4
40-41	3.975354392905742E-5	1.7889094768075837E-4	3.975354392905742E-5	0.3799047425580372	1.9876771964528708E-4
42-43	3.975354392905742E-5	1.9876771964528708E-4	3.975354392905742E-5	0.5035978944932994	1.9876771964528708E-4
44-45	3.975354392905742E-5	1.9876771964528708E-4	3.975354392905742E-5	0.6538265370012073	1.9876771964528708E-4
46-47	3.975354392905742E-5	1.9876771964528708E-4	3.975354392905742E-5	0.8240313353334667	2.1864449160981578E-4
48-49	3.975354392905742E-5	1.9876771964528708E-4	3.975354392905742E-5	0.9972176494604053	2.3852126357434448E-4
50-51	3.975354392905742E-5	1.9876771964528708E-4	3.975354392905742E-5	1.1807001314649699	2.3852126357434448E-4
52-53	3.975354392905742E-5	1.9876771964528708E-4	3.975354392905742E-5	1.3748564400144863	2.3852126357434448E-4
54-55	3.975354392905742E-5	1.9876771964528708E-4	3.975354392905742E-5	1.6000205128286673	2.3852126357434448E-4
56-57	3.975354392905742E-5	1.9876771964528708E-4	7.950708785811484E-5	1.8533897250605147	2.3852126357434448E-4
58-59	3.975354392905742E-5	1.9876771964528708E-4	7.950708785811484E-5	2.11741288706535	2.3852126357434448E-4
60-61	3.975354392905742E-5	1.9876771964528708E-4	7.950708785811484E-5	2.392328520106746	2.3852126357434448E-4
62-63	3.975354392905742E-5	1.9876771964528708E-4	7.950708785811484E-5	2.6815355521906388	2.3852126357434448E-4
64-65	3.975354392905742E-5	1.9876771964528708E-4	7.950708785811484E-5	2.9972581980752127	2.3852126357434448E-4
66-67	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	3.362652897099144	2.3852126357434448E-4
68-69	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	3.751541940585148	2.3852126357434448E-4
70-71	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	4.160466770211397	2.3852126357434448E-4
72-73	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	4.5803437011901025	2.3852126357434448E-4
74-75	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	5.024609431369283	2.3852126357434448E-4
76-77	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	5.523417023819131	2.3852126357434448E-4
78-79	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	6.075713009625526	2.3852126357434448E-4
80-81	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	6.646176365007499	2.3852126357434448E-4
82-83	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	7.2312491477834016	2.3852126357434448E-4
84-85	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	7.849456509424174	2.3852126357434448E-4
86-87	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	8.519562122664329	2.7827480750340194E-4
88-89	3.975354392905742E-5	2.782748075034019E-4	7.950708785811484E-5	9.242361058382452	3.1802835143245934E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2500	0.0	57.032497	1
GTATCAA	4540	0.0	42.083447	1
TCAACGC	5790	0.0	32.544235	4
CAACGCA	6020	0.0	31.194649	5
ATCAACG	6110	0.0	30.762108	3
TATCAAC	6115	0.0	30.736958	2
GTACATG	8650	0.0	30.604141	1
AACGCAG	6380	0.0	29.50603	6
TACATGG	9040	0.0	28.77228	2
ACATGGG	9330	0.0	27.165752	3
ACGCAGA	7630	0.0	23.737123	7
CATGGGG	8230	0.0	23.703045	4
CGCAGAG	7980	0.0	22.517311	8
ATGGGGG	5215	0.0	21.879452	5
TACCGTA	205	6.78554E-7	20.869703	7
TCTATAC	495	0.0	19.177277	3
ATAGGAC	520	0.0	19.168056	3
GCAGAGT	8830	0.0	19.057686	9
GAGTACA	6570	0.0	18.663603	1
GTAAGTA	2025	0.0	18.516726	2
>>END_MODULE