FastQCFastQC Report
Mon 15 Aug 2016
SRR2921409_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921409_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089380
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.052.6176071
GTATCAA55500.043.776171
TCAACGC65350.036.4529764
CAACGCA67550.035.3308565
ATCAACG69050.034.5002253
TATCAAC70050.034.008822
AACGCAG71900.033.5800176
GTACATG117000.027.6604561
ACGCAGA87250.027.1877927
CGCAGAG87950.026.4349738
TACATGG120800.025.888952
TATACCG2651.8189894E-1225.06655
ACATGGG123750.024.3889883
GCAGAGT98000.022.0778799
CATGGGG105650.021.8743084
GTATAGG8150.020.3784541
GAGTACA88550.020.2028941
GTATATG9850.019.751881
ATGGGGG69100.019.5694395
AGTACAT89100.019.1210922
Produced by FastQC (version 0.11.5)