FastQCFastQC Report
Mon 15 Aug 2016
SRR2921409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089380
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.054.977921
GTATCAA53250.041.7694131
TCAACGC70250.031.2130894
CAACGCA70850.030.8061455
TATCAAC71900.030.6948242
ATCAACG72100.030.5438543
AACGCAG76350.028.6473926
GTACATG113850.026.9669361
TACATGG119750.025.2467232
ACATGGG123750.023.9320813
ACGCAGA91850.023.3471417
TATACCG2452.9467628E-1023.2905485
CGCAGAG96300.022.02148
CATGGGG102300.021.990944
ATGGGGG65550.020.8922065
GTATAGG8500.018.4513071
GCAGAGT110100.018.2247459
GTGGTAT17600.018.092321
GAGTACA90850.018.0478841
AGAGTAC95500.017.3016610-11
Produced by FastQC (version 0.11.5)