##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3089380
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4560283940467	33.0	31.0	34.0	30.0	34.0
2	31.585127436573035	34.0	31.0	34.0	29.0	34.0
3	31.658504619049776	34.0	31.0	34.0	28.0	34.0
4	35.156606827259836	37.0	35.0	37.0	33.0	37.0
5	34.90790093805229	37.0	35.0	37.0	32.0	37.0
6	34.86352957551353	37.0	35.0	37.0	32.0	37.0
7	34.73685464397387	37.0	35.0	37.0	32.0	37.0
8	34.69931474923771	37.0	35.0	37.0	32.0	37.0
9	36.31690436268766	39.0	37.0	39.0	32.0	39.0
10-11	36.16969310994439	39.0	37.0	39.0	32.0	39.0
12-13	36.09358414957046	39.0	37.0	39.0	32.0	39.0
14-15	37.33115430927889	40.0	38.0	41.0	32.0	41.0
16-17	37.310552117253295	40.0	38.0	41.0	32.0	41.0
18-19	37.27639477824029	40.0	38.0	41.0	31.0	41.0
20-21	37.23634871721834	40.0	38.0	41.0	31.0	41.0
22-23	37.13225614848287	40.0	38.0	41.0	31.0	41.0
24-25	37.06182227501958	40.0	37.0	41.0	31.0	41.0
26-27	36.92544879555121	40.0	37.0	41.0	30.5	41.0
28-29	36.77718765577559	40.0	37.0	41.0	30.0	41.0
30-31	36.633590396778644	40.0	37.0	41.0	30.0	41.0
32-33	36.59072678013064	40.0	36.5	41.0	30.0	41.0
34-35	36.72866416562547	40.0	37.0	41.0	30.0	41.0
36-37	36.71128074241433	40.0	37.0	41.0	30.0	41.0
38-39	36.633938686726786	40.0	37.0	41.0	30.0	41.0
40-41	36.51346872187947	40.0	37.0	41.0	30.0	41.0
42-43	36.41883290498417	40.0	36.5	41.0	30.0	41.0
44-45	36.286988165910316	40.0	36.0	41.0	29.0	41.0
46-47	36.13173565569791	40.0	36.0	41.0	28.5	41.0
48-49	35.979669221656124	40.0	35.5	41.0	28.0	41.0
50-51	35.82923531582389	40.0	35.0	41.0	28.0	41.0
52-53	35.66172986165509	39.0	35.0	41.0	28.0	41.0
54-55	35.45420424162777	39.0	35.0	41.0	27.0	41.0
56-57	35.197734982423654	39.0	35.0	41.0	26.5	41.0
58-59	34.97010484304294	38.5	35.0	40.5	26.0	41.0
60-61	34.68863558383883	38.0	34.5	40.0	26.0	41.0
62-63	34.384992296188884	37.5	34.0	40.0	26.0	41.0
64-65	34.07107413137911	37.0	34.0	40.0	26.0	41.0
66-67	33.73500038842745	36.5	34.0	39.0	25.0	41.0
68-69	33.36470003690061	36.0	34.0	39.0	24.0	41.0
70-71	32.98163045659647	35.5	33.0	38.5	23.5	40.0
72-73	32.58144368773022	35.0	33.0	37.5	22.5	39.5
74-75	32.173228447131784	35.0	33.0	37.0	21.5	39.0
76-77	29.425267529407193	33.0	29.0	34.5	16.5	36.5
78-79	31.086817743365984	34.5	31.5	36.0	19.5	37.0
80-81	31.286116955505634	35.0	32.0	35.5	20.0	37.0
82-83	31.126332144313746	35.0	32.0	35.0	19.5	37.0
84-85	30.908201160103324	35.0	32.0	35.0	18.5	36.0
86-87	30.67077682253397	35.0	32.0	35.0	17.5	36.0
88-89	30.40758841579864	35.0	32.0	35.0	10.5	36.0
90-91	30.22950268338631	35.0	32.0	35.0	6.0	35.0
92-93	30.087837041736528	34.0	32.0	35.0	4.5	35.0
94-95	29.99582909839515	34.0	31.0	35.0	2.0	35.0
96-97	29.817700315273616	34.0	31.0	35.0	2.0	35.0
98-99	29.564092957162927	34.0	31.0	35.0	2.0	35.0
100-101	28.48614689678835	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52770.0
3	18160.0
4	10261.0
5	7210.0
6	6349.0
7	6309.0
8	6590.0
9	6903.0
10	7995.0
11	8604.0
12	9271.0
13	10120.0
14	10579.0
15	11737.0
16	12919.0
17	13962.0
18	12947.0
19	11433.0
20	11058.0
21	10902.0
22	11663.0
23	13287.0
24	15571.0
25	18636.0
26	22532.0
27	27238.0
28	32788.0
29	39529.0
30	48193.0
31	60477.0
32	77297.0
33	101902.0
34	150554.0
35	221393.0
36	378825.0
37	745767.0
38	783337.0
39	94312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42117491755166	19.225334145524677	13.438568088077158	25.9149228488465
2	15.261846774934906	20.821923217853254	37.75715648916735	26.15907351804449
3	18.425296613626035	24.707853544149234	28.74089895002078	28.125950892203942
4	13.453219920802862	16.456729183398057	35.058281079600796	35.031769816198285
5	14.369446111865496	37.732475728501115	33.66778885722408	14.230289302409311
6	33.91619709556463	34.70578219842142	17.026818638520023	14.35120206749394
7	29.307254703621282	32.04217681569897	20.338935868905523	18.311632611774225
8	27.401375641200353	34.91221215780933	19.34734568059086	18.339066520399452
9	27.499854339705703	15.652104953097384	18.863493645974273	37.98454706122264
10-11	26.184043076289974	25.36937173389844	27.135502863192922	21.31108232661866
12-13	26.581793969777678	22.513394307969385	27.253742831876508	23.651068890376425
14-15	23.808012404465867	24.104188449474137	24.53222668578698	27.555572460273016
16-17	24.047777270304014	27.249426016960705	25.213789058441233	23.489007654294053
18-19	24.01718278297058	26.786160227088263	26.602368798961212	22.594288190979945
20-21	24.469154320809825	25.728928584954247	26.692250149950002	23.109666944285927
22-23	24.347168572745993	25.431143798111556	26.27560413217001	23.946083496972438
24-25	24.12709783152997	25.71202539328221	26.247515230693086	23.91336154449473
26-27	23.716457000836115	26.07559817316715	26.384974367822156	23.822970458174574
28-29	23.794945301925054	26.4913032901391	26.020952772178944	23.6927986357569
30-31	24.59553746073863	25.91065986298097	25.91951410754718	23.574288568733216
32-33	24.222905343621917	25.87765551483841	25.88223639579524	24.017202745744434
34-35	24.353264713832726	26.058338985081004	26.10646112110171	23.48193517998456
36-37	24.21217322850448	26.139567087348066	26.234149998397722	23.414109685749736
38-39	24.334606298881262	26.146381111513207	25.96103611929148	23.557976470314053
40-41	24.453317579651852	25.87383567075885	25.961268602966765	23.711578146622536
42-43	24.097041750347348	25.897438885586983	25.796730723661465	24.208788640404205
44-45	23.9482726555989	25.9221354427579	26.063729142323844	24.06586275931936
46-47	24.071345549271285	25.815976237789144	25.871639582513584	24.241038630425983
48-49	23.795339270013635	26.048868193525248	26.091583674431313	24.06420886202981
50-51	24.056224028579447	25.933223121876026	26.100790071775563	23.909762777768968
52-53	24.320200276500174	25.77422152189035	25.94433936948768	23.961238832121797
54-55	23.539793733276213	25.624326415641868	26.44252455088037	24.39335530020155
56-57	23.786875807955028	25.731357613702045	26.064145660847526	24.4176209174954
58-59	23.98311733359274	25.81658876065425	26.145084038625672	24.055209867127342
60-61	24.23775294058426	25.853283738076577	26.184104019905867	23.724859301433295
62-63	24.073500317113133	25.775551241047097	26.2169843787383	23.933964063101467
64-65	23.698869440905096	25.528001696729103	26.24733064684559	24.525798215520215
66-67	23.772750513482332	25.86453327427032	25.982756448926843	24.379959763320507
68-69	23.686754743030022	25.80614350118438	26.082002711968016	24.425099043817582
70-71	23.699071559928928	25.56987619827576	26.098974671341413	24.6320775704539
72-73	23.629318839784343	25.990138079514104	25.82157982727474	24.558963253426814
74-75	23.52872803470964	25.95151289688562	25.963897170703376	24.555861897701366
76-77	23.450581699079184	25.475853014795735	26.09994568169473	24.97361960443035
78-79	23.3506446475129	25.518618705483398	26.286727967268625	24.844008679735072
80-81	23.712798433529617	25.741391829403447	25.831944492124574	24.71386524494236
82-83	23.63320524446692	25.77637619988209	26.018805073005474	24.571613482645517
84-85	23.568690178606122	25.76354440544496	25.889792640754315	24.777972775194602
86-87	23.202985711418698	26.02934299543983	25.88215342216973	24.885517870971743
88-89	23.087273676134394	25.703296503574574	26.068381061429236	25.141048758861796
90-91	23.368562087513133	25.432912311511117	26.1632762806333	25.035249320342444
92-93	23.474094473691753	25.411687512762132	26.06068790900554	25.053530104540574
94-95	23.306327586357586	25.44455410898353	26.045045689541595	25.204072615117283
96-97	22.949715302756072	25.34894795231317	26.19229211630293	25.50904462862783
98-99	22.696560837783444	25.24483769604697	26.15737814761555	25.901223318554027
100-101	22.768219348354375	25.438650582285977	26.157106753643383	25.636023315716262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	79.0
2	86.0
3	115.0
4	113.0
5	117.0
6	201.0
7	438.5
8	664.0
9	698.5
10	592.5
11	480.5
12	466.0
13	493.0
14	526.5
15	575.0
16	659.5
17	739.0
18	808.5
19	900.0
20	974.5
21	1087.5
22	1302.5
23	1519.0
24	1714.5
25	2093.0
26	2774.5
27	3719.5
28	4959.5
29	6873.5
30	10230.0
31	15074.0
32	22025.0
33	28687.0
34	34685.5
35	42330.5
36	51770.5
37	61055.5
38	69534.5
39	78065.0
40	84701.0
41	90776.5
42	97628.5
43	104960.5
44	111554.5
45	118202.0
46	124066.5
47	129807.5
48	136802.5
49	141076.5
50	141577.5
51	141065.5
52	139371.0
53	137497.5
54	134713.5
55	127405.0
56	117328.5
57	105187.5
58	93033.5
59	79754.5
60	65416.5
61	53763.5
62	43810.5
63	33378.0
64	23576.0
65	16820.5
66	12513.5
67	9356.0
68	6880.5
69	5097.0
70	3762.0
71	2723.0
72	1955.0
73	1463.5
74	1093.0
75	757.0
76	506.5
77	291.0
78	161.5
79	93.0
80	59.0
81	40.0
82	23.5
83	19.0
84	14.5
85	6.0
86	5.0
87	5.0
88	2.5
89	1.5
90	1.0
91	1.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15281383319630476
2	5.17903268617004E-4
3	0.005696935954787045
4	0.00411085719464747
5	0.02518304643650182
6	0.001165282354388259
7	1.6184477144281378E-4
8	0.0018450303944480772
9	0.0
10-11	0.10447079996633629
12-13	2.58951634308502E-4
14-15	0.005502722229055668
16-17	9.710686286568826E-5
18-19	2.751361114527834E-4
20-21	5.502722229055668E-4
22-23	3.3987402002990893E-4
24-25	0.0019097683030252024
26-27	0.0032045264745677125
28-29	0.006295761609125455
30-31	0.015083932698470245
32-33	0.014711689724151772
34-35	0.011814668315325405
36-37	8.739617657911944E-4
38-39	1.9421372573137652E-4
40-41	0.0050171879147272265
42-43	0.008286452297872064
44-45	0.008399743637882035
46-47	0.008852908997921913
48-49	0.010875968640957086
50-51	0.00540561536618998
52-53	0.0016508166687167003
54-55	9.87253105801164E-4
56-57	0.005713120431931326
58-59	0.00953265703798173
60-61	0.007784733506399343
62-63	0.01867688662450071
64-65	0.03512031540309059
66-67	0.037321404294712854
68-69	0.027448873236701216
70-71	0.041982533712265895
72-73	0.026979523399517053
74-75	0.025425813593666042
76-77	0.24425612906149455
78-79	0.07085564093766386
80-81	0.054185629479054045
82-83	0.020683761790391598
84-85	0.03301633337433401
86-87	0.08236280418724792
88-89	0.2646485702632891
90-91	0.32006422000530854
92-93	0.05280994892179013
94-95	0.005211401640458603
96-97	0.010002006875165892
98-99	0.0012138357858211032
100-101	0.001537525328706731
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3089380.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.48467662925059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82725851063297	42.83078176655694
2	13.04756599669488	14.73975092244232
3	4.553617103794964	7.716287688038497
4	2.1106651619903403	4.768809565905968
5	1.1326967438306712	3.199000464714028
6	0.7348481082324897	2.4904594659077235
7	0.49240499352401107	1.9469335780882402
8	0.3573668341686549	1.6148600052828397
9	0.2615287852975325	1.3295131980094648
>10	1.3909805154399588	13.928456499462541
>50	0.06655499427295875	2.5362013826279783
>100	0.022903243329442494	2.1279541210565105
>500	0.001206756593717844	0.44393754840935634
>1k	4.0225219745362146E-4	0.3270537934975318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2368954288562755E-5
2	0.0	0.0	0.0	0.0	6.473790857712551E-5
3	0.0	0.0	0.0	0.0	6.473790857712551E-5
4	0.0	0.0	0.0	0.0	6.473790857712551E-5
5	0.0	0.0	0.0	0.0	6.473790857712551E-5
6	0.0	0.0	0.0	0.0	6.473790857712551E-5
7	0.0	0.0	0.0	0.0	6.473790857712551E-5
8	0.0	0.0	0.0	0.0	6.473790857712551E-5
9	0.0	0.0	0.0	0.0	6.473790857712551E-5
10-11	0.0	0.0	0.0	3.2368954288562755E-5	6.473790857712551E-5
12-13	0.0	0.0	0.0	6.473790857712551E-5	1.4566029429853241E-4
14-15	0.0	0.0	0.0	8.092238572140689E-5	2.2658268001993928E-4
16-17	0.0	0.0	0.0	9.710686286568826E-5	3.560584971741903E-4
18-19	0.0	0.0	0.0	9.710686286568826E-5	4.207964057513158E-4
20-21	0.0	0.0	0.0	1.1329134000996964E-4	4.369808828955972E-4
22-23	0.0	3.2368954288562755E-5	0.0	2.751361114527834E-4	4.5316536003987856E-4
24-25	0.0	6.473790857712551E-5	0.0	4.369808828955972E-4	4.5316536003987856E-4
26-27	1.6184477144281377E-5	6.473790857712551E-5	0.0	9.87253105801164E-4	4.5316536003987856E-4
28-29	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.0037547986974732794	4.693498371841599E-4
30-31	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.013190348872589321	4.855343143284413E-4
32-33	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.03376081932297095	5.502722229055668E-4
34-35	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.06235879043691614	6.473790857712551E-4
36-37	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.10395289669771929	6.473790857712551E-4
38-39	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.16969424285779022	7.121169943483806E-4
40-41	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.2454214114158828	7.444859486369433E-4
42-43	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.3277032932174093	7.444859486369433E-4
44-45	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.43147816066654154	7.444859486369433E-4
46-47	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.5474237549281733	7.768549029255061E-4
48-49	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.6700697227275376	8.739617657911944E-4
50-51	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.8021997941334507	8.739617657911944E-4
52-53	3.2368954288562755E-5	6.473790857712551E-5	0.0	0.941871831888599	8.901462429354757E-4
54-55	3.2368954288562755E-5	6.473790857712551E-5	0.0	1.1081673345460903	9.063307200797571E-4
56-57	3.2368954288562755E-5	6.473790857712551E-5	0.0	1.292913141148062	9.225151972240385E-4
58-59	3.2368954288562755E-5	6.473790857712551E-5	0.0	1.4917070739112703	9.386996743683198E-4
60-61	3.2368954288562755E-5	6.473790857712551E-5	0.0	1.7010694702496942	9.548841515126012E-4
62-63	3.2368954288562755E-5	6.473790857712551E-5	0.0	1.9231690501006675	0.001068175491522571
64-65	3.2368954288562755E-5	6.473790857712551E-5	0.0	2.169577714622352	0.0011005444458111336
66-67	3.2368954288562755E-5	6.473790857712551E-5	0.0	2.455525056807515	0.0011329134000996964
68-69	3.2368954288562755E-5	6.473790857712551E-5	0.0	2.7662184645462844	0.001165282354388259
70-71	3.2368954288562755E-5	6.473790857712551E-5	0.0	3.093403854495077	0.0012138357858211032
72-73	6.473790857712551E-5	6.473790857712551E-5	0.0	3.439525082702678	0.0013918650344081985
74-75	6.473790857712551E-5	6.473790857712551E-5	0.0	3.811120677935379	0.0013918650344081985
76-77	6.473790857712551E-5	6.473790857712551E-5	0.0	4.235348192841282	0.0014242339886967612
78-79	6.473790857712551E-5	6.473790857712551E-5	0.0	4.706251739831293	0.0014242339886967612
80-81	6.473790857712551E-5	6.473790857712551E-5	0.0	5.191899345499745	0.0014727874201296053
82-83	6.473790857712551E-5	6.473790857712551E-5	0.0	5.6995578400844185	0.0015860787601395749
84-85	6.473790857712551E-5	6.473790857712551E-5	0.0	6.240054638794839	0.0015860787601395749
86-87	6.473790857712551E-5	6.473790857712551E-5	0.0	6.81861732774861	0.0016184477144281376
88-89	6.473790857712551E-5	6.473790857712551E-5	0.0	7.44435776757796	0.0016508166687167003
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2870	0.0	54.97792	1
GTATCAA	5325	0.0	41.769413	1
TCAACGC	7025	0.0	31.213089	4
CAACGCA	7085	0.0	30.806145	5
TATCAAC	7190	0.0	30.694824	2
ATCAACG	7210	0.0	30.543854	3
AACGCAG	7635	0.0	28.647392	6
GTACATG	11385	0.0	26.966936	1
TACATGG	11975	0.0	25.246723	2
ACATGGG	12375	0.0	23.932081	3
ACGCAGA	9185	0.0	23.347141	7
TATACCG	245	2.9467628E-10	23.290548	5
CGCAGAG	9630	0.0	22.0214	8
CATGGGG	10230	0.0	21.99094	4
ATGGGGG	6555	0.0	20.892206	5
GTATAGG	850	0.0	18.451307	1
GCAGAGT	11010	0.0	18.224745	9
GTGGTAT	1760	0.0	18.09232	1
GAGTACA	9085	0.0	18.047884	1
AGAGTAC	9550	0.0	17.30166	10-11
>>END_MODULE