FastQCFastQC Report
Mon 15 Aug 2016
SRR2921408_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921408_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3149648
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.049.783061
GTATCAA46600.041.138981
TCAACGC54150.034.4424134
CAACGCA56350.032.842465
ATCAACG56950.032.6656883
TATCAAC59750.031.4526142
AACGCAG59500.031.2548086
GTACATG89150.028.6896671
TACATGG90550.026.5716762
ACATGGG92400.025.4747073
ACGCAGA72700.024.9958137
CGCAGAG73750.024.2550938
CATGGGG80850.022.6572274
GTATAGC9700.020.0571961
GCAGAGT86000.019.3659659
ATGGGGG56950.018.9979235
GAGTACA67350.018.8118611
AGAGTAC73400.017.9070710-11
GTATTAC6650.017.8392431
GTATATA10400.017.7946451
Produced by FastQC (version 0.11.5)