##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921408_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3149648
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.579335532097556	31.0	31.0	34.0	27.0	34.0
2	30.63924508389509	33.0	31.0	34.0	27.0	34.0
3	30.68810292451728	33.0	31.0	34.0	27.0	34.0
4	34.157443942942194	37.0	35.0	37.0	30.0	37.0
5	34.1571731190279	37.0	35.0	37.0	30.0	37.0
6	34.202418174983364	37.0	35.0	37.0	30.0	37.0
7	34.12791937384749	37.0	35.0	37.0	30.0	37.0
8	34.09753470864046	37.0	35.0	37.0	30.0	37.0
9	35.673302857970164	39.0	37.0	39.0	30.0	39.0
10-11	35.570070846012	39.0	37.0	39.0	30.0	39.0
12-13	35.46401439144945	39.0	37.0	39.0	30.0	39.0
14-15	36.61026073389789	40.0	37.0	41.0	30.0	41.0
16-17	36.560423736239734	40.0	37.0	41.0	30.0	41.0
18-19	36.514391925700906	40.0	37.0	41.0	29.0	41.0
20-21	36.455841890903365	40.0	37.0	41.0	29.0	41.0
22-23	36.34643125200022	40.0	37.0	41.0	28.5	41.0
24-25	36.2491808608454	40.0	36.0	41.0	28.0	41.0
26-27	36.10457692415152	40.0	36.0	41.0	27.0	41.0
28-29	35.96558012196918	40.0	36.0	41.0	27.0	41.0
30-31	35.82446006664871	40.0	36.0	41.0	27.0	41.0
32-33	35.64255291384942	40.0	35.5	41.0	26.5	41.0
34-35	35.47961168994122	39.0	35.0	41.0	25.5	41.0
36-37	35.31596657785251	39.0	35.0	41.0	25.0	41.0
38-39	35.544355115238275	39.5	35.0	41.0	26.0	41.0
40-41	35.66789622205402	40.0	35.5	41.0	26.0	41.0
42-43	35.50853539824133	40.0	35.0	41.0	25.0	41.0
44-45	35.39601457051708	40.0	35.0	41.0	25.0	41.0
46-47	35.249726636119334	40.0	35.0	41.0	24.0	41.0
48-49	35.10810604867592	40.0	35.0	41.0	24.0	41.0
50-51	33.39309995910654	37.0	32.5	39.5	20.5	40.0
52-53	33.55629152845016	37.5	33.5	39.5	21.5	40.0
54-55	34.43115278278715	39.0	34.0	40.5	22.0	41.0
56-57	34.4552465862852	39.0	34.0	41.0	22.0	41.0
58-59	34.286580595672916	38.5	34.0	41.0	22.0	41.0
60-61	34.04668188318187	38.0	34.0	40.5	20.5	41.0
62-63	33.76513010342743	37.5	34.0	40.0	20.0	41.0
64-65	33.47620892874379	37.0	34.0	40.0	19.0	41.0
66-67	33.14141437392369	36.5	33.5	39.5	17.5	41.0
68-69	32.777400522217086	36.0	33.0	39.0	14.0	41.0
70-71	32.39429898198148	35.5	33.0	39.0	12.0	41.0
72-73	32.03431859687178	35.0	33.0	38.5	8.0	40.0
74-75	31.62813511224111	35.0	32.0	37.0	6.5	39.5
76-77	31.24266600585208	35.0	32.0	37.0	2.0	39.0
78-79	30.88936795476828	35.0	32.0	36.0	2.0	39.0
80-81	30.537424658247524	35.0	31.5	36.0	2.0	37.0
82-83	30.229934900661917	35.0	31.0	35.0	2.0	37.0
84-85	29.992917145027	35.0	31.0	35.0	2.0	36.5
86-87	29.765595076021192	35.0	31.0	35.0	2.0	36.0
88-89	29.563267228591894	35.0	31.0	35.0	2.0	36.0
90-91	29.310047027477356	35.0	31.0	35.0	2.0	36.0
92-93	29.118250356865275	34.5	31.0	35.0	2.0	35.0
94-95	28.914918428980002	34.0	30.0	35.0	2.0	35.0
96-97	28.666975008000893	34.0	30.0	35.0	2.0	35.0
98-99	28.44756668046715	34.0	30.0	35.0	2.0	35.0
100-101	27.400903053293575	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94437.0
3	16553.0
4	11559.0
5	8950.0
6	8207.0
7	8381.0
8	8953.0
9	9383.0
10	10555.0
11	11391.0
12	11455.0
13	11872.0
14	12829.0
15	13840.0
16	13982.0
17	14603.0
18	13759.0
19	12751.0
20	13073.0
21	13721.0
22	14893.0
23	17176.0
24	19755.0
25	23179.0
26	27902.0
27	32655.0
28	38888.0
29	45821.0
30	54986.0
31	67547.0
32	83624.0
33	108568.0
34	154059.0
35	222252.0
36	364656.0
37	668526.0
38	745863.0
39	139028.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03601121848375	18.17260315137869	13.02415900285227	24.767226627285286
2	16.160297607512618	19.758718076606613	39.206612935506726	24.87437138037404
3	19.181753524166872	23.727902583473128	29.60350998348501	27.486833908874992
4	13.56690532570319	15.84657669672089	36.5613402857682	34.02517769180772
5	14.374361916306292	37.217329320751766	34.27742719572262	14.130881567219317
6	33.436696888201276	34.95882446899554	17.37288902564845	14.23158961715473
7	28.833455798997964	31.95286903075148	21.06235841561396	18.1513167546366
8	26.79465312407609	34.536241746928134	20.109756209869932	18.559348919125853
9	27.012117880598275	15.570042772539578	19.423890189308736	37.99394915755341
10-11	25.664637198088496	25.24503514838028	27.926639347385912	21.163688306145314
12-13	26.21834768420927	22.72824641426592	27.657039868837362	23.396366032687443
14-15	23.4310275987438	24.387233687067276	24.990034060484277	27.191704653704647
16-17	23.607522332997956	27.341709477931236	25.91440795959226	23.13636022947854
18-19	23.5672550091254	26.810066870907107	27.1236999043794	22.498978215588096
20-21	24.058452266528285	26.01747368792122	27.090869624108173	22.83320442144232
22-23	23.982163410691822	25.796443641005922	26.713970856718205	23.50742209158405
24-25	23.790138469769996	25.940073458586994	26.702880868099005	23.566907203544
26-27	23.515135000286627	26.20270694515175	26.75044467067692	23.5317133838847
28-29	23.546974626586742	26.623228876117032	26.493299750446525	23.3364967468497
30-31	24.108667881560258	26.132564387613925	26.399723489456367	23.359044241369446
32-33	23.780061854229775	26.133951875631084	26.31884775857799	23.767138511561154
34-35	24.07360513478118	26.30681517794729	26.372003325770386	23.247576361501142
36-37	24.059184508040367	26.158794999250702	26.47688220248361	23.305138290225326
38-39	23.83937885774968	26.26566417631671	26.48189422603851	23.413062739895096
40-41	24.063535768636175	26.09656733236324	26.427537034855643	23.41235986414494
42-43	23.79639667560694	26.085631854556052	26.274418025976953	23.843553443860056
44-45	23.703197783631794	26.110943747472977	26.487257441575863	23.698601027319366
46-47	23.923831381227878	26.015635401537008	26.24184559265527	23.818687624579844
48-49	23.615674685957035	26.230105336594985	26.343867523043123	23.810352454404857
50-51	23.80989949838266	26.22044949596492	26.438745931198763	23.530905074453653
52-53	24.03674604810734	26.023811250836697	26.36122691282803	23.578215788227926
54-55	23.396014285505228	25.903103909680382	26.711690101503947	23.989191703310443
56-57	23.53912420152662	26.02570641250844	26.484754038222363	23.950415347742577
58-59	23.69032749050782	26.00834713599316	26.52474584122625	23.77657953227277
60-61	23.772271217114575	26.035919029143884	26.665357920786725	23.526451832954816
62-63	23.734643734643733	25.95837015390269	26.68767593338652	23.619310178067057
64-65	23.427527030470944	25.785158702104695	26.69838661901409	24.088927648410273
66-67	23.501901642682054	25.94729801309755	26.48828394046451	24.062516403755886
68-69	23.54122376913258	25.889839304248234	26.661131193109288	23.907805733509896
70-71	23.44235268821035	25.69811913701431	26.604691151941108	24.254837022834234
72-73	23.343532736489582	25.97467973513574	26.510569885686948	24.171217642687733
74-75	23.382961687957852	26.025286666732406	26.54466981355648	24.04708183175326
76-77	23.35580580577396	25.665124321738286	26.61332290824373	24.36574696424402
78-79	23.262159399596708	25.64759317665682	26.764575569440524	24.325671854305952
80-81	23.452675082410767	25.812918845499876	26.530547630074892	24.203858442014464
82-83	23.4207542781066	25.873098402736254	26.626116441121884	24.08003087803526
84-85	23.369146044077443	25.869120589328347	26.578878540119227	24.182854826474976
86-87	23.203649340372625	25.976854604789253	26.56218051524274	24.257315539595385
88-89	23.07984841090656	25.76059876959963	26.688960241198316	24.470592578295495
90-91	23.203968738135906	25.61978118986405	26.768488259934653	24.407761812065395
92-93	23.349505448342583	25.431364249707215	26.853629894597486	24.365500407352716
94-95	23.20700588136159	25.377525423119224	26.902299884989038	24.513168810530146
96-97	23.109839607901428	25.196260944541503	27.00892750575057	24.684971941806502
98-99	22.830886453899566	25.17938469533841	27.00038619444755	24.98934265631447
100-101	23.054772012046897	25.115921559890353	27.098933364100404	24.730373063962347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	90.0
1	66.0
2	82.5
3	124.0
4	128.5
5	162.5
6	229.5
7	430.5
8	686.0
9	786.0
10	697.5
11	585.0
12	622.5
13	688.5
14	765.5
15	860.0
16	959.0
17	1126.5
18	1270.0
19	1389.5
20	1545.5
21	1696.5
22	1870.0
23	2119.5
24	2455.0
25	3001.0
26	3889.5
27	5293.5
28	7316.0
29	10293.0
30	14731.0
31	21015.5
32	29500.0
33	35980.0
34	41261.0
35	48602.0
36	57978.5
37	67745.5
38	76249.5
39	84421.5
40	91168.0
41	97617.0
42	104254.0
43	111080.5
44	117631.0
45	125488.5
46	131410.5
47	134814.5
48	139031.5
49	140549.5
50	139762.0
51	137798.0
52	135084.0
53	131706.5
54	126854.0
55	119241.0
56	108905.5
57	97567.0
58	87256.0
59	75030.5
60	60719.5
61	49592.0
62	40855.5
63	31374.0
64	22334.0
65	16160.5
66	12175.0
67	9240.5
68	6870.5
69	5147.5
70	3856.0
71	2816.5
72	1940.0
73	1418.0
74	1101.5
75	810.0
76	588.0
77	393.0
78	270.5
79	197.5
80	141.5
81	105.0
82	90.0
83	77.5
84	64.0
85	57.0
86	49.0
87	42.5
88	37.0
89	32.5
90	29.0
91	27.0
92	20.5
93	21.0
94	18.5
95	16.5
96	13.0
97	7.5
98	9.0
99	6.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05172006522633641
2	0.05962571055559224
3	0.05076757783726944
4	0.05657775091057794
5	0.049211848435126715
6	0.01806551081263684
7	0.014795304110173583
8	0.021240468776193403
9	0.021113470457651138
10-11	0.022605700700522726
12-13	0.028114252767293364
14-15	0.04524315098068102
16-17	0.029971603175973947
18-19	0.02365343682849639
20-21	0.02719351495786196
22-23	0.048021239198793
24-25	0.04265556024038242
26-27	0.030781217456680873
28-29	0.019795862902775168
30-31	0.012731581433861815
32-33	0.009778870527754214
34-35	0.009604247839758601
36-37	0.001269983185422625
38-39	0.007127780628184483
40-41	0.003651201658090047
42-43	0.22031033309118986
44-45	0.19452967442711058
46-47	0.20105421304221932
48-49	0.19838724835283178
50-51	0.19735538701467592
52-53	0.20141933320802835
54-55	0.20072084245604588
56-57	0.21015046760780887
58-59	0.2074517533387858
60-61	0.21240468776193402
62-63	0.20924560458819527
64-65	0.21021396676707998
66-67	0.2004350962393258
68-69	0.19956198279934773
70-71	0.1832585736564848
72-73	0.19049747781339374
74-75	0.19097372150792724
76-77	0.18890999883161547
78-79	0.21464303312624142
80-81	0.22245342971659054
82-83	0.2210088238431723
84-85	0.2164051347960153
86-87	0.2343595220799277
88-89	0.23296254057596277
90-91	0.23142268596363785
92-93	0.23520088594027014
94-95	0.2468053572970694
96-97	0.24717047746287837
98-99	0.23648674391551056
100-101	0.24639261276180702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3149648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.3997152666332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9946291426935	47.108533925453386
2	12.362170269453987	14.933431287053182
3	3.988342444423089	7.226842440869471
4	1.848371699529325	4.465644974338964
5	1.0097272521631218	3.049361926380625
6	0.6489203384972411	2.3516762205576356
7	0.4172213132434194	1.7640033966151318
8	0.29493252858981406	1.425107259975434
9	0.22967351742227832	1.2484993550932557
>10	1.1421625996666789	11.902979279895598
>50	0.04813777128764213	1.950090343945439
>100	0.014026023745664942	1.5677708708939395
>500	8.952089950411765E-4	0.3883524694838359
>1k	7.898902891922746E-4	0.617706249444083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.174957963556562E-5	0.0	0.0	0.0
6	0.0	3.174957963556562E-5	0.0	0.0	0.0
7	0.0	3.174957963556562E-5	0.0	0.0	0.0
8	0.0	3.174957963556562E-5	0.0	0.0	0.0
9	0.0	3.174957963556562E-5	0.0	0.0	0.0
10-11	0.0	3.174957963556562E-5	0.0	3.174957963556562E-5	0.0
12-13	0.0	3.174957963556562E-5	0.0	6.349915927113125E-5	0.0
14-15	0.0	3.174957963556562E-5	0.0	6.349915927113125E-5	3.174957963556562E-5
16-17	0.0	3.174957963556562E-5	0.0	7.937394908891405E-5	9.524873890669688E-5
18-19	0.0	3.174957963556562E-5	0.0	1.1112352872447968E-4	9.524873890669688E-5
20-21	0.0	3.174957963556562E-5	0.0	1.9049747781339375E-4	1.5874789817782814E-4
22-23	0.0	3.174957963556562E-5	0.0	3.174957963556562E-4	1.7462268799561093E-4
24-25	0.0	4.762436945334844E-5	0.0	5.556176436223984E-4	2.698714269023078E-4
26-27	0.0	6.349915927113125E-5	0.0	0.0010159865483381	2.8574621672009063E-4
28-29	0.0	6.349915927113125E-5	0.0	0.0035559529191833503	2.8574621672009063E-4
30-31	0.0	6.349915927113125E-5	0.0	0.013620569663657654	3.0162100653787345E-4
32-33	0.0	6.349915927113125E-5	0.0	0.036543766160536034	3.1749579635565627E-4
34-35	0.0	6.349915927113125E-5	0.0	0.06849971806373284	3.4924537599122186E-4
36-37	0.0	6.349915927113125E-5	0.0	0.11774331607849511	3.4924537599122186E-4
38-39	0.0	6.349915927113125E-5	0.0	0.19383118367512814	3.651201658090047E-4
40-41	0.0	6.349915927113125E-5	0.0	0.27822156634646156	3.809949556267875E-4
42-43	0.0	6.349915927113125E-5	0.0	0.37000960107288183	3.968697454445703E-4
44-45	0.0	7.937394908891405E-5	0.0	0.483942967595109	4.2861932508013597E-4
46-47	1.587478981778281E-5	9.524873890669688E-5	0.0	0.618656433988814	4.6036890471570156E-4
48-49	3.174957963556562E-5	9.524873890669688E-5	0.0	0.7538620188668702	5.397428538046156E-4
50-51	3.174957963556562E-5	9.524873890669688E-5	0.0	0.8965446297491021	5.714924334401813E-4
52-53	3.174957963556562E-5	9.524873890669688E-5	0.0	1.047117011170772	6.032420130757469E-4
54-55	3.174957963556562E-5	9.524873890669688E-5	0.0	1.2272641260229715	6.826159621646609E-4
56-57	3.174957963556562E-5	9.524873890669688E-5	0.0	1.434080887769046	6.984907519824437E-4
58-59	3.174957963556562E-5	9.524873890669688E-5	0.0	1.6471046923338735	6.984907519824437E-4
60-61	3.174957963556562E-5	1.1112352872447968E-4	0.0	1.8679706430686858	7.302403316180094E-4
62-63	3.174957963556562E-5	1.269983185422625E-4	0.0	2.099536202140684	7.302403316180094E-4
64-65	3.174957963556562E-5	1.269983185422625E-4	1.587478981778281E-5	2.3591842644003393	7.302403316180094E-4
66-67	3.174957963556562E-5	1.269983185422625E-4	3.174957963556562E-5	2.6668059414893346	7.302403316180094E-4
68-69	3.174957963556562E-5	1.269983185422625E-4	3.174957963556562E-5	2.9974936881835683	7.302403316180094E-4
70-71	3.174957963556562E-5	1.269983185422625E-4	3.174957963556562E-5	3.336769696169223	7.302403316180094E-4
72-73	3.174957963556562E-5	1.269983185422625E-4	3.174957963556562E-5	3.688428040212748	7.937394908891407E-4
74-75	3.174957963556562E-5	1.269983185422625E-4	3.174957963556562E-5	4.063358826129142	7.937394908891407E-4
76-77	3.174957963556562E-5	1.5874789817782814E-4	3.174957963556562E-5	4.5000266696468945	7.937394908891407E-4
78-79	3.174957963556562E-5	1.9049747781339375E-4	3.174957963556562E-5	4.9796993187810195	7.937394908891407E-4
80-81	3.174957963556562E-5	1.9049747781339375E-4	3.174957963556562E-5	5.471563806495203	8.254890705247063E-4
82-83	3.174957963556562E-5	1.9049747781339375E-4	3.174957963556562E-5	5.982747913417627	8.889882297958375E-4
84-85	3.174957963556562E-5	1.9049747781339375E-4	3.174957963556562E-5	6.529062930206804	8.889882297958375E-4
86-87	3.174957963556562E-5	2.3812184726674216E-4	3.174957963556562E-5	7.120811595454477	9.683621788847516E-4
88-89	3.174957963556562E-5	2.53996637084525E-4	3.174957963556562E-5	7.76266109736707	0.0010159865483381
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2545	0.0	49.78306	1
GTATCAA	4660	0.0	41.13898	1
TCAACGC	5415	0.0	34.442413	4
CAACGCA	5635	0.0	32.84246	5
ATCAACG	5695	0.0	32.665688	3
TATCAAC	5975	0.0	31.452614	2
AACGCAG	5950	0.0	31.254808	6
GTACATG	8915	0.0	28.689667	1
TACATGG	9055	0.0	26.571676	2
ACATGGG	9240	0.0	25.474707	3
ACGCAGA	7270	0.0	24.995813	7
CGCAGAG	7375	0.0	24.255093	8
CATGGGG	8085	0.0	22.657227	4
GTATAGC	970	0.0	20.057196	1
GCAGAGT	8600	0.0	19.365965	9
ATGGGGG	5695	0.0	18.997923	5
GAGTACA	6735	0.0	18.811861	1
AGAGTAC	7340	0.0	17.90707	10-11
GTATTAC	665	0.0	17.839243	1
GTATATA	1040	0.0	17.794645	1
>>END_MODULE