FastQCFastQC Report
Mon 15 Aug 2016
SRR2921408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3149648
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.048.6195871
GTATCAA46400.043.949511
TCAACGC59150.033.3798074
CAACGCA61250.032.2958765
TATCAAC61850.032.229592
ATCAACG61300.032.209063
AACGCAG65000.030.5038646
GTACATG84950.024.844731
ACGCAGA80000.024.190047
CGCAGAG82000.023.5420558
TACATGG89900.022.7542632
ACATGGG89150.022.360073
CATGGGG75900.020.7606774
GCAGAGT92450.019.7495179
AGAGTAC78400.018.49762510-11
ATGGGGG50150.018.2049775
TAAGGTG14150.017.4745795
CAGAGTA87700.016.75294110-11
AGTACAC30200.016.5016573
GAGTACT42050.016.47381412-13
Produced by FastQC (version 0.11.5)