##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3149648
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.479406905152576	33.0	31.0	34.0	30.0	34.0
2	31.61313930953554	34.0	31.0	34.0	29.0	34.0
3	31.687894329779073	34.0	31.0	34.0	29.0	34.0
4	35.18437774633864	37.0	35.0	37.0	33.0	37.0
5	34.93515370606493	37.0	35.0	37.0	32.0	37.0
6	34.88907331866926	37.0	35.0	37.0	32.0	37.0
7	34.760494823548534	37.0	35.0	37.0	32.0	37.0
8	34.722860141831724	37.0	35.0	37.0	32.0	37.0
9	36.35312231716052	39.0	37.0	39.0	32.0	39.0
10-11	36.20425584065267	39.0	37.0	39.0	32.0	39.0
12-13	36.13687132657363	39.0	37.0	39.0	32.0	39.0
14-15	37.38838498778276	40.0	38.0	41.0	32.0	41.0
16-17	37.36931841272421	40.0	38.0	41.0	32.0	41.0
18-19	37.33257621169096	40.0	38.0	41.0	31.5	41.0
20-21	37.29752880321865	40.0	38.0	41.0	31.0	41.0
22-23	37.19330604562795	40.0	38.0	41.0	31.0	41.0
24-25	37.12659049519185	40.0	37.5	41.0	31.0	41.0
26-27	36.98934150736844	40.0	37.0	41.0	30.5	41.0
28-29	36.83840543451205	40.0	37.0	41.0	30.0	41.0
30-31	36.70065353334722	40.0	37.0	41.0	30.0	41.0
32-33	36.65073081182405	40.0	37.0	41.0	30.0	41.0
34-35	36.78397268520165	40.0	37.0	41.0	30.0	41.0
36-37	36.75584478011511	40.0	37.0	41.0	30.0	41.0
38-39	36.66955545508577	40.0	37.0	41.0	30.0	41.0
40-41	36.550828219534374	40.0	37.0	41.0	30.0	41.0
42-43	36.45259057520079	40.0	37.0	41.0	30.0	41.0
44-45	36.32186310978243	40.0	36.0	41.0	29.5	41.0
46-47	36.16715105941998	40.0	36.0	41.0	28.5	41.0
48-49	36.0146097278172	40.0	36.0	41.0	28.0	41.0
50-51	35.865439566580136	40.0	35.0	41.0	28.0	41.0
52-53	35.70186223984395	39.5	35.0	41.0	28.0	41.0
54-55	35.49857857131971	39.0	35.0	41.0	27.0	41.0
56-57	35.241905920915606	39.0	35.0	41.0	26.5	41.0
58-59	35.02608005720005	39.0	35.0	41.0	26.0	41.0
60-61	34.75442795512387	38.0	35.0	40.0	26.0	41.0
62-63	34.46310270227022	37.5	34.0	40.0	26.0	41.0
64-65	34.15657130574591	37.0	34.0	40.0	26.0	41.0
66-67	33.82175008127892	36.5	34.0	39.5	25.0	41.0
68-69	33.455014655605964	36.0	34.0	39.0	24.0	41.0
70-71	33.07335914997485	35.5	33.5	39.0	23.5	40.0
72-73	32.67505194231228	35.0	33.0	38.0	22.0	40.0
74-75	32.26292207891167	35.0	33.0	37.0	21.5	39.0
76-77	29.487775776848714	33.0	29.0	34.5	15.0	36.5
78-79	31.144930798616226	34.5	31.5	36.0	19.0	38.0
80-81	31.336795572076625	35.0	32.0	36.0	20.0	37.0
82-83	31.16699104153861	35.0	32.0	35.0	19.5	37.0
84-85	30.934515857010055	35.0	32.0	35.0	18.0	36.5
86-87	30.684951302494753	35.0	32.0	35.0	16.0	36.0
88-89	30.402603719526752	35.0	32.0	35.0	10.0	36.0
90-91	30.216182728990667	35.0	32.0	35.0	4.5	35.5
92-93	30.068540357525666	34.0	32.0	35.0	2.0	35.0
94-95	29.972401995397583	34.0	31.5	35.0	2.0	35.0
96-97	29.787513715818402	34.0	31.0	35.0	2.0	35.0
98-99	29.53336626823061	34.0	31.0	35.0	2.0	35.0
100-101	28.449097803945076	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53206.0
3	18350.0
4	10328.0
5	7068.0
6	6230.0
7	6091.0
8	6721.0
9	7228.0
10	8074.0
11	8626.0
12	9455.0
13	10259.0
14	11312.0
15	12307.0
16	13496.0
17	14664.0
18	13510.0
19	11978.0
20	11546.0
21	11447.0
22	12079.0
23	13656.0
24	16107.0
25	19193.0
26	22918.0
27	27404.0
28	33005.0
29	40081.0
30	49000.0
31	60042.0
32	76821.0
33	101842.0
34	147792.0
35	218953.0
36	377001.0
37	742655.0
38	827792.0
39	111409.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59917769276999	19.757091843386405	13.71761728053523	25.92611318330837
2	15.072476652056507	21.109747637424654	37.75704168895761	26.060734021561228
3	17.719957122427154	24.794851897103985	29.4918258276413	27.99336515282756
4	13.125191895965477	16.982003135107778	35.6126525761448	34.28015239278195
5	14.08425252765205	37.55711750485587	34.278950651238006	14.079679316254081
6	33.36372858269979	35.614448115087086	17.033319945453652	13.988503356759477
7	28.546024364000154	32.1032483957726	21.080267090248526	18.270460149978714
8	26.580459085847853	35.144448180360286	19.87508282779407	18.4000099059978
9	26.717715757443372	15.544530690413657	19.343304394649817	38.394449157493156
10-11	25.520791535436167	25.594607449511585	27.888287996801363	20.996313018250888
12-13	26.03848244436514	22.897444917584338	27.692447146407655	23.371625491642863
14-15	23.194174888294608	24.611589101967237	24.966283982927273	27.227952026810883
16-17	23.479113830007456	27.660427654680973	25.76768993575147	23.0927685795601
18-19	23.455660070195357	27.07474206960577	27.07229734460002	22.397300515598854
20-21	23.919245727036422	26.15425499320714	27.081173687440646	22.845325592315792
22-23	23.837173953179143	25.859990186190913	26.712943861741813	23.58989199888813
24-25	23.63887275370606	26.145060435365775	26.6941293740214	23.52193743690676
26-27	23.354736821551043	26.41088950551735	26.748523486342318	23.48585018658929
28-29	23.408202656787246	26.77927493845737	26.514577339548612	23.29794506520677
30-31	23.9473850502718	26.272227604574017	26.411263753972857	23.369123591181324
32-33	23.641437405491093	26.292688177915856	26.265538201445835	23.80033621514721
34-35	23.86141867797934	26.47854899706916	26.402070956126337	23.257961368825157
36-37	23.870974706398098	26.308276478780606	26.5193329820752	23.301415832746095
38-39	23.75258005594923	26.429202566260727	26.42912319213523	23.38909418565481
40-41	23.96915502073906	26.217486219600843	26.406738992627858	23.406619767032232
42-43	23.696073304330902	26.263950713010864	26.199811328602895	23.84016465405534
44-45	23.61653323866947	26.25353679713748	26.39454827587192	23.73538168832113
46-47	23.76600246780455	26.19879988238873	26.215136545289546	23.820061104517173
48-49	23.5114730694684	26.401602520950767	26.316091129169518	23.770833280411317
50-51	23.676735572549624	26.33772720128657	26.348141668505605	23.637395557658206
52-53	23.95113285002336	26.171144562619926	26.22143665522024	23.656285932136473
54-55	23.294947178489824	26.052695621832058	26.62817838298726	24.02417881669086
56-57	23.42933119540239	26.14763630301996	26.382771663418758	24.040260838158893
58-59	23.501568722956954	26.19313280055071	26.43318027660709	23.872118199885247
60-61	23.659104013940325	26.246751786993432	26.48881192243407	23.605332276632172
62-63	23.57945902964737	26.19973058815236	26.457123059990177	23.763687322210092
64-65	23.289243150374396	25.967653083472907	26.513930307412743	24.22917345873995
66-67	23.333198348098335	26.22221586997587	26.26082164718512	24.183764134740674
68-69	23.381481683201567	26.10309908920558	26.39349132673906	24.12192790085379
70-71	23.315087589575104	25.905303494102018	26.349178973292993	24.430429943029893
72-73	23.23230574973419	26.18791594206949	26.191441009426857	24.388337298769468
74-75	23.25329442008838	26.20780053892092	26.16616675426381	24.37273828672689
76-77	23.195282505299417	25.847735497199874	26.33841185083248	24.61857014666823
78-79	23.100414330280426	25.862298486190106	26.42298352750399	24.614303656025484
80-81	23.322165346351603	25.97903866154424	26.16004327846439	24.53875271363977
82-83	23.192819865977437	26.05437497737393	26.271855198217096	24.480949958431538
84-85	23.146449457254363	26.031845544772686	26.187419340854294	24.634285657118657
86-87	22.968446800147817	26.165566362826688	26.143610066342333	24.722376770683166
88-89	22.843954369040826	25.977692578029497	26.227137407301772	24.951215645627904
90-91	22.921516870707674	25.791738827510702	26.33555091071962	24.95119339106201
92-93	23.017535801182035	25.63738357657156	26.392502903785857	24.95257771846055
94-95	22.84947632072385	25.606982326846417	26.41063510350629	25.132906248923437
96-97	22.639470073178813	25.46860207716504	26.48370349284239	25.40822435681375
98-99	22.356965193874302	25.453023192018843	26.44422275971245	25.74578885439441
100-101	22.476903067097428	25.482262625505758	26.40818781647803	25.632646490918788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	74.0
2	81.5
3	106.0
4	102.5
5	106.5
6	181.0
7	387.5
8	583.5
9	619.5
10	529.0
11	439.5
12	443.5
13	469.0
14	506.0
15	567.5
16	622.0
17	696.5
18	802.0
19	864.0
20	983.0
21	1153.0
22	1301.5
23	1637.0
24	2126.0
25	2701.5
26	3686.0
27	5364.5
28	7608.0
29	10888.5
30	15631.5
31	22056.5
32	30563.5
33	37127.0
34	42220.0
35	49300.5
36	58560.5
37	67764.0
38	75878.0
39	83815.5
40	90316.5
41	97268.0
42	104174.5
43	110415.0
44	116719.5
45	124793.5
46	131264.0
47	135105.5
48	139432.0
49	141195.0
50	140565.5
51	138957.0
52	136279.5
53	133250.0
54	128804.0
55	120349.0
56	109790.5
57	98555.0
58	87656.0
59	75179.5
60	60714.0
61	49546.5
62	40684.5
63	30780.0
64	21516.5
65	15198.5
66	11319.0
67	8719.5
68	6461.0
69	4731.0
70	3486.5
71	2468.5
72	1663.5
73	1162.0
74	879.5
75	631.0
76	423.5
77	252.0
78	141.0
79	75.0
80	42.0
81	26.0
82	19.5
83	10.0
84	6.0
85	7.0
86	6.5
87	6.5
88	6.0
89	1.5
90	0.5
91	2.5
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1536044662768665
2	4.1274453526235315E-4
3	0.0068579092012821755
4	0.004921184843512672
5	0.027812631760755484
6	0.0010477361279736656
7	3.174957963556562E-5
8	0.0013017327650581905
9	0.0
10-11	0.10501173464463331
12-13	1.269983185422625E-4
14-15	0.0057307991242195955
16-17	1.269983185422625E-4
18-19	3.0162100653787345E-4
20-21	6.032420130757469E-4
22-23	1.4287310836004531E-4
24-25	0.0013811067141471047
26-27	0.0026510898995697296
28-29	0.006508663825290953
30-31	0.014827053689809145
32-33	0.015049300747258106
34-35	0.012001341102243806
36-37	5.556176436223984E-4
38-39	2.2224705744895937E-4
40-41	0.004968809212966021
42-43	0.008159641966340366
44-45	0.008223141125611497
46-47	0.009207378094314031
48-49	0.011429848668803625
50-51	0.0058101730733085095
52-53	0.0015081050326893673
54-55	0.0010001117585203172
56-57	0.0058895470223974235
58-59	0.008604136081238285
60-61	0.0071119058383667
62-63	0.017970262073730144
64-65	0.03376567794242404
66-67	0.03668663926889608
68-69	0.02551078723717698
70-71	0.039448852697190284
72-73	0.024510675478656662
74-75	0.024224929261936572
76-77	0.23991569851615166
78-79	0.06854734243318619
80-81	0.05276780135431007
82-83	0.019875236851864084
84-85	0.031162212412307662
86-87	0.07896120455365171
88-89	0.26263252274539883
90-91	0.31624168796005137
92-93	0.05087870136599392
94-95	0.004873560474059324
96-97	0.00976299573793643
98-99	9.524873890669688E-4
100-101	0.0013176075548759734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3149648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.899998188954754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.59844893665743	46.481469507714785
2	12.56096690980171	15.04803790297286
3	4.115402137833229	7.395377418090927
4	1.8473039762495738	4.4261401932719355
5	1.0345602565582266	3.0985078747101165
6	0.6341044737672856	2.2789714098161107
7	0.4393282000711731	1.8421030872034017
8	0.29683766558973773	1.4224460504991077
9	0.22763333842819675	1.227171290363524
>10	1.1802937430888398	12.22407704965302
>50	0.0481258750015861	1.9349370510455703
>100	0.015240836893877778	1.6245336933409393
>500	0.0011159591288906194	0.495965115997749
>1k	6.376909302622914E-4	0.5002623553199366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.174957963556562E-5
2	0.0	0.0	0.0	0.0	3.174957963556562E-5
3	0.0	0.0	0.0	0.0	3.174957963556562E-5
4	0.0	0.0	0.0	3.174957963556562E-5	3.174957963556562E-5
5	0.0	3.174957963556562E-5	0.0	3.174957963556562E-5	3.174957963556562E-5
6	0.0	3.174957963556562E-5	0.0	3.174957963556562E-5	6.349915927113125E-5
7	0.0	3.174957963556562E-5	0.0	3.174957963556562E-5	6.349915927113125E-5
8	0.0	3.174957963556562E-5	0.0	3.174957963556562E-5	6.349915927113125E-5
9	0.0	3.174957963556562E-5	0.0	3.174957963556562E-5	6.349915927113125E-5
10-11	0.0	3.174957963556562E-5	0.0	6.349915927113125E-5	7.937394908891405E-5
12-13	0.0	3.174957963556562E-5	0.0	9.524873890669688E-5	1.1112352872447968E-4
14-15	0.0	3.174957963556562E-5	0.0	9.524873890669688E-5	1.5874789817782814E-4
16-17	0.0	3.174957963556562E-5	0.0	1.1112352872447968E-4	1.5874789817782814E-4
18-19	0.0	3.174957963556562E-5	0.0	1.4287310836004531E-4	1.5874789817782814E-4
20-21	0.0	6.349915927113125E-5	0.0	2.222470574489594E-4	1.5874789817782814E-4
22-23	0.0	6.349915927113125E-5	0.0	3.4924537599122186E-4	2.222470574489594E-4
24-25	0.0	6.349915927113125E-5	0.0	5.873672232579641E-4	2.8574621672009063E-4
26-27	0.0	6.349915927113125E-5	0.0	0.0010477361279736656	2.8574621672009063E-4
28-29	0.0	6.349915927113125E-5	0.0	0.0035877024988189158	2.8574621672009063E-4
30-31	0.0	6.349915927113125E-5	0.0	0.013747567982199914	2.8574621672009063E-4
32-33	0.0	6.349915927113125E-5	0.0	0.037464503969967436	2.8574621672009063E-4
34-35	0.0	6.349915927113125E-5	0.0	0.06981732561860882	2.8574621672009063E-4
36-37	0.0	6.349915927113125E-5	0.0	0.119505417748269	3.1749579635565627E-4
38-39	0.0	6.349915927113125E-5	0.0	0.19648227357469789	3.1749579635565627E-4
40-41	0.0	7.937394908891405E-5	0.0	0.28130127557111145	3.1749579635565627E-4
42-43	0.0	9.524873890669688E-5	0.0	0.37364492794115406	3.1749579635565627E-4
44-45	0.0	9.524873890669688E-5	0.0	0.4885942810117194	3.1749579635565627E-4
46-47	0.0	9.524873890669688E-5	0.0	0.6241649860555846	3.1749579635565627E-4
48-49	0.0	9.524873890669688E-5	0.0	0.7601166860550765	3.1749579635565627E-4
50-51	0.0	9.524873890669688E-5	0.0	0.9030056692049397	3.1749579635565627E-4
52-53	0.0	9.524873890669688E-5	0.0	1.0539590455822365	3.3337058617343904E-4
54-55	0.0	1.269983185422625E-4	0.0	1.2341855343835246	3.809949556267875E-4
56-57	0.0	1.269983185422625E-4	0.0	1.4418912843593952	3.809949556267875E-4
58-59	0.0	1.269983185422625E-4	0.0	1.6558834511031075	3.968697454445703E-4
60-61	0.0	1.269983185422625E-4	0.0	1.8776383900677156	4.1274453526235315E-4
62-63	0.0	1.269983185422625E-4	0.0	2.110235810477869	4.1274453526235315E-4
64-65	0.0	1.5874789817782814E-4	0.0	2.3695187525717163	4.1274453526235315E-4
66-67	0.0	1.5874789817782814E-4	0.0	2.677680172514516	4.1274453526235315E-4
68-69	0.0	1.5874789817782814E-4	0.0	3.008336169629114	4.4449411489791874E-4
70-71	0.0	1.5874789817782814E-4	0.0	3.3487234129020136	5.238680639868328E-4
72-73	0.0	1.5874789817782814E-4	0.0	3.7038424611258147	5.397428538046156E-4
74-75	0.0	1.5874789817782814E-4	0.0	4.082218076432667	5.714924334401813E-4
76-77	0.0	1.5874789817782814E-4	0.0	4.522378373710332	6.032420130757469E-4
78-79	0.0	1.5874789817782814E-4	0.0	5.007353202643597	6.032420130757469E-4
80-81	1.587478981778281E-5	1.5874789817782814E-4	3.174957963556562E-5	5.505551731495075	6.191168028935297E-4
82-83	3.174957963556562E-5	1.5874789817782814E-4	3.174957963556562E-5	6.023736620727142	6.349915927113125E-4
84-85	3.174957963556562E-5	1.5874789817782814E-4	3.174957963556562E-5	6.577258792093593	6.349915927113125E-4
86-87	3.174957963556562E-5	1.9049747781339375E-4	3.174957963556562E-5	7.174944628733115	6.349915927113125E-4
88-89	3.174957963556562E-5	1.9049747781339375E-4	3.174957963556562E-5	7.823906036484077	6.349915927113125E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2630	0.0	48.619587	1
GTATCAA	4640	0.0	43.94951	1
TCAACGC	5915	0.0	33.379807	4
CAACGCA	6125	0.0	32.295876	5
TATCAAC	6185	0.0	32.22959	2
ATCAACG	6130	0.0	32.20906	3
AACGCAG	6500	0.0	30.503864	6
GTACATG	8495	0.0	24.84473	1
ACGCAGA	8000	0.0	24.19004	7
CGCAGAG	8200	0.0	23.542055	8
TACATGG	8990	0.0	22.754263	2
ACATGGG	8915	0.0	22.36007	3
CATGGGG	7590	0.0	20.760677	4
GCAGAGT	9245	0.0	19.749517	9
AGAGTAC	7840	0.0	18.497625	10-11
ATGGGGG	5015	0.0	18.204977	5
TAAGGTG	1415	0.0	17.474579	5
CAGAGTA	8770	0.0	16.752941	10-11
AGTACAC	3020	0.0	16.501657	3
GAGTACT	4205	0.0	16.473814	12-13
>>END_MODULE