FastQCFastQC Report
Mon 15 Aug 2016
SRR2921407_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921407_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054828
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.046.2195361
GTATCAA41950.040.155021
TCAACGC49550.033.044354
CAACGCA51250.032.0392725
TATCAAC51800.031.9734082
ATCAACG52100.031.5155223
AACGCAG54750.030.242226
GTACATG87600.026.4337771
TACATGG89950.025.693372
ACATGGG94050.023.7655723
ACGCAGA68550.023.7415357
CGCAGAG72800.021.9655468
CATGGGG77700.021.5002464
GAGTACA64900.020.1793821
ATGGGGG51750.019.716445
AGTACAT67600.018.32262
GTATAGG7000.018.302451
GCAGAGT82200.018.2411259
GTATAGC9550.017.887211
AGAGTAC68200.016.9766710-11
Produced by FastQC (version 0.11.5)