##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921407_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3054828
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.58243017282806	31.0	31.0	34.0	27.0	34.0
2	30.637615603889973	33.0	31.0	34.0	27.0	34.0
3	30.68889279527358	33.0	31.0	34.0	27.0	34.0
4	34.165152997157286	37.0	35.0	37.0	30.0	37.0
5	34.163210498267006	37.0	35.0	37.0	30.0	37.0
6	34.20814756182672	37.0	35.0	37.0	30.0	37.0
7	34.13299766795381	37.0	35.0	37.0	30.0	37.0
8	34.105375818213005	37.0	35.0	37.0	30.0	37.0
9	35.6747014889218	39.0	37.0	39.0	30.0	39.0
10-11	35.57462171356292	39.0	37.0	39.0	30.0	39.0
12-13	35.46795809780453	39.0	36.0	39.0	30.0	39.0
14-15	36.61486244070043	40.0	37.0	41.0	30.0	41.0
16-17	36.56097593710677	40.0	37.0	41.0	30.0	41.0
18-19	36.51564670744146	40.0	37.0	41.0	29.0	41.0
20-21	36.456836358708244	40.0	37.0	41.0	29.0	41.0
22-23	36.34176064904473	40.0	37.0	41.0	28.5	41.0
24-25	36.24886163803657	40.0	36.0	41.0	28.0	41.0
26-27	36.10491589051823	40.0	36.0	41.0	27.0	41.0
28-29	35.969960829218536	40.0	36.0	41.0	27.0	41.0
30-31	35.82980203795435	40.0	36.0	41.0	27.0	41.0
32-33	35.65792247550435	39.5	35.5	41.0	26.5	41.0
34-35	35.49879060294066	39.0	35.0	41.0	25.5	41.0
36-37	35.33484176523196	39.0	35.0	41.0	25.0	41.0
38-39	35.569864980941645	39.5	35.0	41.0	26.5	41.0
40-41	35.696080597663766	40.0	35.5	41.0	26.5	41.0
42-43	35.54446878842279	40.0	35.0	41.0	25.5	41.0
44-45	35.43645354173786	40.0	35.0	41.0	25.0	41.0
46-47	35.29546164301231	40.0	35.0	41.0	24.5	41.0
48-49	35.160458133813094	40.0	35.0	41.0	24.0	41.0
50-51	33.44615114173368	37.0	32.5	39.5	21.5	40.0
52-53	33.61128744400666	37.5	33.5	39.5	22.0	40.0
54-55	34.48935930271688	39.0	34.0	40.5	22.5	41.0
56-57	34.51488496242669	39.0	34.0	41.0	22.0	41.0
58-59	34.34793137289563	38.5	34.0	41.0	22.0	41.0
60-61	34.113311289539055	38.0	34.0	40.5	22.0	41.0
62-63	33.832416751450495	37.5	34.0	40.0	20.5	41.0
64-65	33.54831875968139	37.0	34.0	40.0	20.0	41.0
66-67	33.213066333030866	36.5	33.5	39.5	18.5	41.0
68-69	32.84620754425454	36.0	33.0	39.0	17.5	41.0
70-71	32.463751314312944	35.5	33.0	39.0	14.0	41.0
72-73	32.09841879804689	35.0	33.0	38.5	11.5	40.0
74-75	31.68758535668784	35.0	32.0	37.0	7.5	39.5
76-77	31.302617856062597	35.0	32.0	37.0	4.5	39.0
78-79	30.946595192920846	35.0	32.0	36.0	2.0	39.0
80-81	30.586988367266503	35.0	31.5	36.0	2.0	37.0
82-83	30.28120814003276	35.0	31.0	35.5	2.0	37.0
84-85	30.038095598180977	35.0	31.0	35.0	2.0	36.5
86-87	29.80611707107569	35.0	31.0	35.0	2.0	36.0
88-89	29.596622952257867	35.0	31.0	35.0	2.0	36.0
90-91	29.337116525054768	35.0	31.0	35.0	2.0	36.0
92-93	29.14166280392873	34.5	31.0	35.0	2.0	35.0
94-95	28.930810016144935	34.0	30.0	35.0	2.0	35.0
96-97	28.678027862779835	34.0	30.0	35.0	2.0	35.0
98-99	28.44952956434863	34.0	30.0	35.0	2.0	35.0
100-101	27.395660737691287	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90221.0
3	15628.0
4	11056.0
5	8417.0
6	7748.0
7	7971.0
8	8582.0
9	9103.0
10	9983.0
11	10693.0
12	11174.0
13	11425.0
14	11978.0
15	13003.0
16	13178.0
17	13662.0
18	13046.0
19	12081.0
20	12671.0
21	13094.0
22	14837.0
23	16433.0
24	19450.0
25	22869.0
26	27341.0
27	32453.0
28	38593.0
29	45189.0
30	54443.0
31	66189.0
32	82211.0
33	106928.0
34	150139.0
35	216680.0
36	353472.0
37	645961.0
38	722733.0
39	134178.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32514985788802	18.089096581779405	12.733711818253873	24.852041742078708
2	16.059854490855564	19.60537883343749	39.10430513684227	25.230461538864674
3	19.33301018252562	23.592541766696034	29.42579594991599	27.648652100862353
4	13.71704343941142	15.548008964197582	36.35776738811294	34.37718020827806
5	14.42637758823354	37.20614065684578	34.20652286763553	14.160958887285155
6	33.726876256458276	34.815926816011945	17.18546797545691	14.27172895207287
7	29.148375538256833	31.811273534003163	20.77900562615902	18.261345301580985
8	26.770076231101847	34.837731415844885	19.916676871414285	18.475515481638986
9	27.047467246777884	15.6531146216251	19.418216727726122	37.8812014038709
10-11	25.774154531637084	25.19516349465277	27.807990292412903	21.222691681297242
12-13	26.118282080341125	22.650424718794277	27.621702457478186	23.609590743386413
14-15	23.45583086779358	24.350155333917158	24.78751708738589	27.406496710903372
16-17	23.682847290282293	27.407078014551328	25.66742166274433	23.242653032422044
18-19	23.676552470572364	26.917582974398503	26.88285870003515	22.523005854993986
20-21	24.09876660442523	26.07457348514678	26.872961156266395	22.953698754161593
22-23	24.007673651993496	25.858897162255328	26.523996128785036	23.60943305696614
24-25	23.782511407188103	25.960574072421416	26.56562786817196	23.691286652218515
26-27	23.581234454059988	26.274668844462667	26.553398789463273	23.590697912014072
28-29	23.69443875108783	26.56032167719519	26.339628621438642	23.405610950278337
30-31	24.127816373434257	26.13028162097392	26.288280088788195	23.453621916803623
32-33	23.766968970184113	26.137975987266195	26.29498786399026	23.800067178559434
34-35	24.01875224561251	26.234745180782546	26.35967285057541	23.38682972302953
36-37	23.97115475267646	26.18622134985649	26.416466761643377	23.42615713582368
38-39	23.956620092711418	26.28347366629128	26.349291569546658	23.410614671450645
40-41	24.01302048892142	26.065104408778012	26.373888442949344	23.54798665935122
42-43	23.77369670773292	26.076574254742457	26.226467342927233	23.923261694597386
44-45	23.74532375752586	26.1059057190794	26.38306072677952	23.765709796615226
46-47	23.784780568101542	26.05914839058453	26.207650015097826	23.948421026216096
48-49	23.66125003628782	26.20100385643177	26.290029976610143	23.847716130670268
50-51	23.728827736462733	26.127343642240263	26.43112307364035	23.712705547656647
52-53	23.865672608762328	26.015668631327916	26.379509570035236	23.739149189874517
54-55	23.434014142790552	25.90332752825261	26.63710328987013	24.025555039086704
56-57	23.553927169111297	26.057369018223714	26.360619176885862	24.028084635779123
58-59	23.664934759887384	26.024824507476023	26.533521548887602	23.77671918374899
60-61	23.774376121706357	25.923131219365043	26.62337289526016	23.679119763668435
62-63	23.68118326442604	25.952294444795292	26.6344725659841	23.73204972479457
64-65	23.436252566938894	25.753407535002147	26.650644845232385	24.159695052826574
66-67	23.47503059730599	25.932475657468938	26.439172293257833	24.153321451967233
68-69	23.466567863863382	25.890992494168845	26.546659968282725	24.095779673685044
70-71	23.420329411286254	25.695303661424536	26.60263679239946	24.281730134889752
72-73	23.39274325105095	25.883174289336804	26.45641819482782	24.267664264784433
74-75	23.32072619975774	25.928634942959583	26.52357334993438	24.227065507348293
76-77	23.326547724727433	25.56568376746086	26.637348551726557	24.470419956085145
78-79	23.220264414015247	25.580285975532334	26.760017887004135	24.439431723448283
80-81	23.306016101603433	25.73270760828159	26.53022487581908	24.43105141429589
82-83	23.34760315407059	25.79242619489584	26.63093059519015	24.229040055843424
84-85	23.341251761222733	25.714221033883884	26.59643593644063	24.34809126845275
86-87	23.119865091597724	25.84336619959106	26.640931075365533	24.39583763344568
88-89	23.074812877567293	25.505058294257203	26.79680193734475	24.623326890830754
90-91	23.23303337467356	25.335035507258386	26.861922269760658	24.570008848307396
92-93	23.244731906850365	25.297265989686135	26.934669574106067	24.523332529357425
94-95	23.15323203674665	25.25081833893114	26.90259288893498	24.693356735387226
96-97	23.040009294336066	25.10087734988023	26.988658087565643	24.870455268218063
98-99	22.866471402738497	25.03161375611458	27.005030799928527	25.0968840412184
100-101	23.011621570309245	25.01409970530508	27.053006523195634	24.921272201190035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	64.5
2	87.0
3	136.0
4	132.5
5	155.5
6	240.0
7	393.5
8	602.5
9	713.5
10	642.0
11	557.5
12	597.0
13	667.5
14	735.5
15	863.0
16	949.0
17	1019.0
18	1167.5
19	1270.5
20	1369.5
21	1529.5
22	1641.0
23	1830.5
24	2134.0
25	2572.0
26	3369.5
27	4529.0
28	6375.5
29	9285.5
30	13354.0
31	19111.5
32	26755.5
33	33818.0
34	40403.5
35	47843.0
36	56897.5
37	66257.0
38	74606.5
39	82054.5
40	88578.5
41	95444.5
42	102481.5
43	109507.5
44	116054.5
45	123174.5
46	127566.0
47	130284.0
48	133824.0
49	135282.5
50	134786.0
51	132312.0
52	129346.5
53	125586.5
54	120235.5
55	113309.5
56	104556.0
57	94092.0
58	83372.0
59	72109.0
60	59544.0
61	48485.5
62	39430.5
63	30712.0
64	22754.0
65	16851.5
66	12928.0
67	10338.5
68	8221.5
69	6445.5
70	4983.0
71	3814.5
72	2849.5
73	2128.5
74	1667.5
75	1333.0
76	1039.5
77	713.0
78	474.5
79	337.0
80	225.5
81	165.5
82	139.5
83	99.0
84	69.5
85	59.0
86	55.5
87	43.5
88	36.0
89	33.0
90	32.5
91	32.0
92	23.0
93	16.0
94	9.5
95	11.5
96	12.0
97	10.5
98	11.5
99	11.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052441577725488964
2	0.05970876265374024
3	0.05100123476673645
4	0.05872671063640899
5	0.05037926848909333
6	0.019902920884580084
7	0.01715317523605257
8	0.022718136667596343
9	0.022947282138306966
10-11	0.023634718550438846
12-13	0.030279937201047
14-15	0.04602550454559144
16-17	0.031065578814912002
18-19	0.02515689917730229
20-21	0.028168525363784804
22-23	0.04985550741318333
24-25	0.04519076033085987
26-27	0.031949425630510134
28-29	0.019182749405203828
30-31	0.01204650474593005
32-33	0.009231288962913788
34-35	0.008527485017159723
36-37	0.0014403429587525058
38-39	0.0061541926419425245
40-41	0.003404446993415014
42-43	0.2287853849709378
44-45	0.2020735700995277
46-47	0.20873515628375802
48-49	0.2057235300972755
50-51	0.20408677673505676
52-53	0.20886609655273555
54-55	0.20783494193453772
56-57	0.21881755699502559
58-59	0.2144146904506571
60-61	0.22055251555897745
62-63	0.21770456470871685
64-65	0.21911217260022495
66-67	0.20809682247249273
68-69	0.20726207825776116
70-71	0.18929052634059923
72-73	0.19767070355515923
74-75	0.1972287801473602
76-77	0.19554292418427488
78-79	0.22276213259797278
80-81	0.23101136954355533
82-83	0.22981653958913562
84-85	0.22503721977145688
86-87	0.2431233444239741
88-89	0.24086462478411222
90-91	0.24068458191426814
92-93	0.24462915751721537
94-95	0.2553989946406148
96-97	0.25569361024581416
98-99	0.24549663679919131
100-101	0.254629720560372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3054828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.35567272662487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3163169312539	50.25138617377777
2	11.906755606526614	15.08721022886012
3	3.7989934362786304	7.220633545183946
4	1.6622027003292041	4.212398811494767
5	0.8922044869320414	2.826310773964633
6	0.5512136501760014	2.0953506973799425
7	0.3788459984953571	1.6801430166124283
8	0.26871170455688953	1.3619528649375845
9	0.20515659490447144	1.1698050676028802
>10	0.9627560351164383	10.415574034508268
>50	0.04310383285669465	1.8533224619147086
>100	0.012806959966917398	1.361964737660201
>500	8.285000950623082E-4	0.36956367967525305
>1k	1.0356251181508436E-4	0.09438390642729381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.273506724437513E-5
5	0.0	6.547013448875026E-5	0.0	0.0	3.273506724437513E-5
6	3.273506724437513E-5	6.547013448875026E-5	0.0	0.0	3.273506724437513E-5
7	3.273506724437513E-5	6.547013448875026E-5	0.0	0.0	3.273506724437513E-5
8	3.273506724437513E-5	6.547013448875026E-5	0.0	0.0	3.273506724437513E-5
9	3.273506724437513E-5	6.547013448875026E-5	0.0	3.273506724437513E-5	3.273506724437513E-5
10-11	6.547013448875026E-5	6.547013448875026E-5	0.0	3.273506724437513E-5	3.273506724437513E-5
12-13	6.547013448875026E-5	6.547013448875026E-5	0.0	6.547013448875026E-5	3.273506724437513E-5
14-15	6.547013448875026E-5	6.547013448875026E-5	0.0	6.547013448875026E-5	3.273506724437513E-5
16-17	6.547013448875026E-5	6.547013448875026E-5	0.0	1.1457273535531298E-4	3.273506724437513E-5
18-19	6.547013448875026E-5	9.82052017331254E-5	0.0	1.6367533622187568E-4	3.273506724437513E-5
20-21	6.547013448875026E-5	1.3094026897750053E-4	0.0	2.1277793708843835E-4	3.273506724437513E-5
22-23	6.547013448875026E-5	1.3094026897750053E-4	0.0	2.6188053795500105E-4	9.82052017331254E-5
24-25	6.547013448875026E-5	1.3094026897750053E-4	0.0	5.728636767765648E-4	1.6367533622187568E-4
26-27	6.547013448875026E-5	1.3094026897750053E-4	0.0	9.493169500868788E-4	1.6367533622187568E-4
28-29	6.547013448875026E-5	1.3094026897750053E-4	0.0	0.003764532733103141	1.6367533622187568E-4
30-31	6.547013448875026E-5	1.3094026897750053E-4	0.0	0.016531208958409444	1.6367533622187568E-4
32-33	6.547013448875026E-5	1.3094026897750053E-4	0.0	0.04334122903155267	1.964104034662508E-4
34-35	6.547013448875026E-5	1.3094026897750053E-4	0.0	0.08170672784196034	1.964104034662508E-4
36-37	6.547013448875026E-5	1.3094026897750053E-4	0.0	0.14064621641545777	1.964104034662508E-4
38-39	6.547013448875026E-5	1.3094026897750053E-4	0.0	0.23156786568670967	1.964104034662508E-4
40-41	9.82052017331254E-5	1.3094026897750053E-4	0.0	0.3307387519035442	1.964104034662508E-4
42-43	9.82052017331254E-5	1.3094026897750053E-4	0.0	0.4395992180247137	1.964104034662508E-4
44-45	9.82052017331254E-5	1.3094026897750053E-4	0.0	0.5757934652949364	1.964104034662508E-4
46-47	9.82052017331254E-5	1.3094026897750053E-4	0.0	0.7299265294150767	2.2914547071062593E-4
48-49	9.82052017331254E-5	1.3094026897750053E-4	0.0	0.8841741662705724	2.2914547071062593E-4
50-51	9.82052017331254E-5	1.3094026897750053E-4	0.0	1.0463436893992069	2.2914547071062593E-4
52-53	9.82052017331254E-5	1.3094026897750053E-4	0.0	1.218792023642575	2.455130043328135E-4
54-55	9.82052017331254E-5	1.3094026897750053E-4	0.0	1.4249574771476496	2.6188053795500105E-4
56-57	9.82052017331254E-5	1.3094026897750053E-4	0.0	1.659471498886353	2.6188053795500105E-4
58-59	9.82052017331254E-5	1.3094026897750053E-4	0.0	1.9014327484231517	2.6188053795500105E-4
60-61	9.82052017331254E-5	1.3094026897750053E-4	0.0	2.1483697281810956	2.6188053795500105E-4
62-63	9.82052017331254E-5	1.3094026897750053E-4	0.0	2.4111832155525614	2.946156051993762E-4
64-65	9.82052017331254E-5	1.6367533622187568E-4	0.0	2.7064862571640695	2.946156051993762E-4
66-67	9.82052017331254E-5	1.6367533622187568E-4	0.0	3.054623697307999	2.946156051993762E-4
68-69	9.82052017331254E-5	1.6367533622187568E-4	0.0	3.4260030351954347	2.946156051993762E-4
70-71	9.82052017331254E-5	1.8004286984406326E-4	0.0	3.803487463123947	2.946156051993762E-4
72-73	9.82052017331254E-5	1.964104034662508E-4	0.0	4.191103394364593	3.2735067244375136E-4
74-75	1.1457273535531296E-4	1.964104034662508E-4	0.0	4.6087864848691975	3.2735067244375136E-4
76-77	1.3094026897750053E-4	1.964104034662508E-4	0.0	5.097062747886296	3.2735067244375136E-4
78-79	1.3094026897750053E-4	1.964104034662508E-4	0.0	5.625472203345	3.6008573968812646E-4
80-81	1.3094026897750053E-4	1.964104034662508E-4	0.0	6.164520555658125	3.6008573968812646E-4
82-83	1.3094026897750053E-4	1.964104034662508E-4	0.0	6.713733146350629	3.6008573968812646E-4
84-85	1.3094026897750053E-4	1.964104034662508E-4	0.0	7.305288546523732	3.6008573968812646E-4
86-87	1.3094026897750053E-4	2.2914547071062593E-4	0.0	7.95882779652406	3.6008573968812646E-4
88-89	1.3094026897750053E-4	2.2914547071062593E-4	0.0	8.66169879286166	3.6008573968812646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2505	0.0	46.219536	1
GTATCAA	4195	0.0	40.15502	1
TCAACGC	4955	0.0	33.04435	4
CAACGCA	5125	0.0	32.039272	5
TATCAAC	5180	0.0	31.973408	2
ATCAACG	5210	0.0	31.515522	3
AACGCAG	5475	0.0	30.24222	6
GTACATG	8760	0.0	26.433777	1
TACATGG	8995	0.0	25.69337	2
ACATGGG	9405	0.0	23.765572	3
ACGCAGA	6855	0.0	23.741535	7
CGCAGAG	7280	0.0	21.965546	8
CATGGGG	7770	0.0	21.500246	4
GAGTACA	6490	0.0	20.179382	1
ATGGGGG	5175	0.0	19.71644	5
AGTACAT	6760	0.0	18.3226	2
GTATAGG	700	0.0	18.30245	1
GCAGAGT	8220	0.0	18.241125	9
GTATAGC	955	0.0	17.88721	1
AGAGTAC	6820	0.0	16.97667	10-11
>>END_MODULE