FastQCFastQC Report
Mon 15 Aug 2016
SRR2921407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054828
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.047.434521
GTATCAA43800.040.475621
TCAACGC56700.031.0503434
ATCAACG57250.031.0007113
CAACGCA56850.030.8616095
TATCAAC60700.029.2392062
AACGCAG60650.029.0819156
GTACATG84800.027.407651
TACATGG89050.025.899122
ACATGGG91350.023.8959793
ACGCAGA75200.023.0121027
CATGGGG75650.022.0805194
CGCAGAG77450.021.9752798
ATGGGGG47300.018.8981535
AGAGTAC76550.018.04154410-11
GCAGAGT91850.017.8571749
GAGTACA65950.017.2963811
AGTACAC25600.017.2392563
GTACACT27750.016.5876274
TACACTG36150.016.572385
Produced by FastQC (version 0.11.5)